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All Journal VALENSI Jurnal Penelitian Saintek Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA JURNAL KIMIA SAINS DAN APLIKASI Alchemy Jurnal Penelitian Kimia INDONESIAN JOURNAL OF PHARMACY Alotrop Jurnal Pendidikan dan Ilmu Kimia Molekul: Jurnal Ilmiah Kimia Greensphere: Journal of Environmental Chemistry

Purbowatiningrum Ria Sarjono

Chemistry Department, Faculty Of Sciences And Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH., Tembalang, Semarang|Diponegoro University|Indonesia

Author-ID : 364991

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Energy Engineering Environmental Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology

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Encapsulation of Cinnamaldehyde using Chitosan: Stability, Mucoadhesive and Cinnamaldehyde Release Purbowatiningrum Ria Sarjono; Ngadiwiyana Ngadiwiyana; Enny Fachriyah; Ismiyarto Ismiyarto; Nor Basid Adiwibawa Prasetya; Khikmah Khikmah
Jurnal Kimia Sains dan Aplikasi Vol 21, No 4 (2018): volume 21 Issue 4 Year 2018
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Cinnamaldehyde contained in cinnamon oil is useful as an antidiabetic; however, it has very low oral bioavailability. One effort to increase oral bioavailability of cinnamaldehyde is by encapsulation using chitosan. Encapsulation of cinnamaldehyde in chitosan has been successfully made in the form of powder. The aim of this study was to obtain data of stability, mucoadhesive and cinnamaldehyde release from cinnamaldehyde encapsulated chitosan. Stability tests were performed physically and chemically. Mucoadhesive tests were carried out in vitro in two steps, which were granule fabrication and then mucoadhesive test. The results showed that the levels of cinnamaldehyde decreased within 4 weeks, from the first to the fourth week 100%; 99.87%; 98.85%; 97.33%, respectively. Cinnamaldehyde powder did not change significantly in specific gravity, pH, centrifugation and organoleptic after a month. The release precentage of cinnamaldehyde in acidic media pH 1.2 for 180 minutes was 83.4%, whereas in alkaline media pH 7.4 for 360 minutes was 61%. The cinnamaldehyde encapsulated chitosan nanoparticle powder performed mucoadhesive capacity in the gastric mucosa and in the intestinal mucosa of 91.5% and 84.61%, respectively.

Penentuan Kandungan Total Flavonoid dan Fenolik Ekstrak Daun Jambu Biji (Psidium Guajava L.) dan Uji Aktivitas Antioksidan Menggunakan Metode DPPH Enny Fachriyah; Lolyta Sari Br Tampubolon; Ngadiwiyana Ngadiwiyana; Ismiyarto Ismiyarto; Purbowatiningrum Ria Sarjono
Jurnal Penelitian Saintek Vol 28, No 1 (2023)
Publisher : Directorate of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.58488

Daun jambu biji (Psidium guajava L.) merupakan salah satu tanaman yang mengandung flavonoid, tanin, saponin, minyak atsiri, triterpenoid dan alkaloid. Flavonoid dan fenolik pada daun jambu biji dipengaruhi oleh lokasi dan lingkungan tumbuhnya. Penelitian ini bertujuan untuk memperoleh informasi tentang kandungan metabolit sekunder, kadar total flavonoid, kadar total fenolik, dan aktivitas antioksidan daun jambu biji yang berasal dari daerah kabupaten Asahan, Sumatera Utara menggunakan pelarut air, etil asetat, dan n-heksana. Kadar total flavonoid ditentukan dengan Kolorimetri AlCl3, kadar total fenolik ditentukan dengan Folin-Ciocalteu, aktivitas antioksidan ditentukan dengan metode DPPH. Hasil uji skrining fitokimia menunjukkan daun jambu biji mengandung saponin, tanin, kuinon, steroid, triterpenoid, dan flavonoid. Kadar total flavonoid ekstrak etil asetat dan air masing-masing 28,7055 mg QE/g dan 10,6624 mg QE/g. Kadar total fenolik ekstrak etil asetat dan air masing-masing 160,4550 mg GAE/g dan 179,8201 mg GAE/g. Ekstrak etil asetat daun jambu biji memiliki nilai IC50 sebesar 66,23 ppm dan ekstrak air memiliki nilai IC50 sebesar 46,81 ppm. Ekstrak air daun jambu biji memiliki aktivitas antioksidan yang sangat kuat.

Isolasi Bakteri Termofilik Sumber Air Panas Gedongsongo dengan Media Pengaya Minimal YT (Yeast Tripton) serta Identifikasi Genotipik dan Fenotipik Annisa Rachma; Purbowatiningrum Ria Sarjono; Agustina L. N. Aminin
Jurnal Kimia Sains dan Aplikasi Vol 12, No 3 (2009): Volume 12 Issue 3 Year 2009
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Eksplorasi mikroorganisme baru merupakan suatu hal yang penting, karena mikroorganisme mempunyai potensi yang sangat luas sebagai sumber biomolekul baru seperti enzim, antimikroba, antikanker, agen bioremediasi, dan penghasil bahan bakar hayati. Hingga saat ini, kurang dari 1% bakteri di alam yang berhasil dikultivasi. Kebutuhan industri terhadap biomolekul yang tahan terhadap kondisi ekstrem sangatlah tinggi. Salah satu sumber biomolekul yang potensial adalah bakteri termofilik. Indonesia kaya akan habitat termofilik, khususnya sumber air panas, namun eksplorasinya masih sangat terbatas. Penelitian ini bertujuan untuk mendapatkan isolat tunggal bakteri termofilik dari sumber air panas Gedong Songo dengan media minimal YT (Yeast Tripton) dalam kondisi anaerob serta melakukan identifikasi bakteri termofilik tersebut secara genotipik dan fenotipik. Pada penelitian ini dilakukan isolasi bakteri termofilik sumber air panas Gedongsongo dengan media pengaya minimal YT pada kondisi anaerob serta identifikasi bakteri secara genotipik dan fenotipik. Identifikasi genotipik dilakukan dengan menggunakan analisis urutan nukleotida gen 16S rRNA. Identifikasi fenotipik dilakukan melalui uji morfologi meliputi uji pewarnaan gram dan uji mikroskop. Selain itu dilakukan uji potensi enzim ekstraseluler termostabil, meliputi amilase, protease, β-galaktosidase, dan selulase. Hasil penelitian diperoleh isolat tunggal bakteri termofilik anaerob yang diberi nama isolat GS Ytan, dengan karakteristik bentuk batang, gram positif, dan memiliki kemiripan dengan bakteri kelompok Anoxybacillus sp. dengan tingkat kekerabatan sebesar 97-99 %. Isolat GS YTan memiliki hubungan terdekat dengan Anoxybacillus sp. AF/04 dan Anoxybacillus sp. HT14. Isolat GS YTan memiliki potensi enzim ekstraseluler termostabil amilase, protease dan β-galaktosidase.

Antibacterial Activity of Cinnamic Acid - Chitosan Encapsulation Nur Amaliyah; Purbowatiningrum Ria Sarjono; Ngadiwiyana Ngadiwiyana; Ismiyarto Ismiyarto
Jurnal Kimia Sains dan Aplikasi Vol 21, No 1 (2018): Volume 21 Issue 1 Year 2018
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

The biggest cinnamon bark is cinnamic acid. The inhibitory activity of bacterial growth of S. aureus and E. coli and its abundance in nature becomes the superiority of cinnamic acid as antibacterial. However, the use of cinnamic acid as an antibacterial is still less than optimal because of low solubility in water. One of the latest technologies to address the problem is encapsulation in nanometer size. Encapsulation of cinnamic acid using chitosan nanoparticles as an encapsulation material was shown to increase the activity of cinnamic acid as antibacterial by protecting the active compound, as well as increasing its solubility in water through chitosan-TPP modification. Emulsion formation was performed using Corning LSE Compact at 6,000 rpm for 30 minutes. The formation of chitosan nanoparticles as encapsulation material was done using freeze dryer with freeze drying method. Chitosan was obtained from shrimp chitin with Deacetylation Degree (% DD) of 85.98%. The synthesis of chitosan nanoparticles yielded an average particle size of 330 nm with a percentage volume of 29.9% and 79.1% with an average size of 5570 nm. The morphological form of nanometer-sized chitosan was smaller and uniform than that of chitosan morphology. Encapsulation efficiency value of 67.304% makes cinnamic acid has higher activity inhibition of bacterial growth than pure cinnamic acid and chitosan nanoparticles.

Synthesis of Nano Chitosan as Carrier Material of Cinnamon’s Active Component Ngadiwiyana Ngadiwiyana; Enny Fachriyah; Purbowatiningrum Ria Sarjono; Nor Basid Adiwibawa Prasetya; Ismiyarto Ismiyarto; Agus Subagio
Jurnal Kimia Sains dan Aplikasi Vol 21, No 2 (2018): Volume 21 Issue 2 Year 2018
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Development and innovation to improve the efficacy of active ingredients of a plant can be done by using nanoparticle encapsulation of chitosan, which has dual function of protecting natural extracts degradation and delivering natural extracts to the target site. Chitosan is a natural polymer that is nontoxic, mucoadhesive, biodegradable, and biocompatible. This polymer also has a low level of immunogenicity and can be prepared into nanoparticles in mild conditions that make it suitable for natural extracts delivery systems. This paper reported synthesis of chitosan nanoparticles for cinnamon’s natural extract delivery. Chitosan synthesis was carried out by chitin deacetylation isolated from shrimp shells. Chitosan characterization was done by measuring deacetylation degree by FTIR. Chitosan nanoparticles were prepared by ionic gelation method using tripolyphosphate as crosslinker. Morphology and particle size of nano chitosan were characterized using a scanning electron microscope (SEM) and a transmission electron microscope (TEM). The result found that the yield of deproteinated chitin was 62.60%. Further process of demineralization resulted a yield of 52.60%, then depigmentation with a yield of 75.56%, and deacetylation with a yield of 79.02%. FTIR analysis showed that deacetylation degree of chitin into chitosan was found of 87.78%. Characterization by SEM found that nano chitosan has a particle size of 87 nm. While TEM images showed that the nano chitosan has a uniform shape and a lower physical aggregation.

Antioxidant from Turmeric Fermentation Products (Curcuma longa) by Aspergillus Oryzae Sulasiyah Sulasiyah; Purbowatiningrum Ria Sarjono; Agustina L. N. Aminin
Jurnal Kimia Sains dan Aplikasi Vol 21, No 1 (2018): Volume 21 Issue 1 Year 2018
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

The antioxidant capacity of natural materials can be improved by fermentation. In this study, turmeric rhizome was fermented by Aspergillus oryzae. Fermentation products were obtained with fermentation time of 14, 21 and 28 hours. Furthermore, the fermentation product was extracted using ethanol and determined its antioxidant capacity by DPPH damping method, total phenolate determination and phytochemical screening. The results showed that the antioxidant capacity of fermentation products was higher than without fermentation. Antioxidant activity increased with length of fermentation with the value of antioxidant capacity without fermentation and with incubation for 14, 21, and 28 days respectively of 17.0; 27.3; 33.3; and 34.1 mg quercetin/gram extract. The total phenolate of fermentation products was 261; 324,3; 361; 374.3 mg of gallic acid/gram extract. Ethanol extracts of fermented and non-fermented products all contain entirely positive alkaloids, saponins, flavonoids, tannins, quinones, and steroids.

Uji Aktivitas Trichoderma Viride dalam Hidrolisis Selulosa Eceng Gondok (Eichhornia Crassipes) dengan Variasi Temperatur dan Waktu Inkubasi Wirnia Sinar Setyani; Purbowatiningrum Ria Sarjono; Nies Suci Mulyani
Jurnal Kimia Sains dan Aplikasi Vol 14, No 1 (2011): Volume 14 issue 1 Year 2011
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Telah dilakukan pengujian aktivitas Trichoderma viride dalam menghidrolisis selulosa eceng gondok (Eichhornia crassipes) dengan variasi temperatur dan waktu inkubasi untuk memperoleh temperatur dan waktu inkubasi optimum. Aktivitas Trichoderma viride didasarkan pada kemampuan enzim selulase yang dihasilkan untuk memecah ikatan β-1,4-glikosida pada rantai selulosa dalam eceng gondok dan diamati berdasarkan banyaknya glukosa yang dihasilkan sebagai hasil reaksi hidrolisis. Hasil pengujian aktivitas Trichoderma viride menunjukkan bahwa kadar glukosa paling tinggi diperoleh pada temperatur 35oC dan waktu inkubasi optimum dihasilkannya glukosa pada jam ke-96 sebesar 1,3864 mg/L.

Studi Filogeni dan Uji Potensi Antibakteri serta Enzim Ekstraseluler Isolat Geobacillus sp. dari Sumber Air Panas Gedongsongo Ifsantin Nihaya; Purbowatiningrum Ria Sarjono; Agustina L. N. Aminin
Jurnal Kimia Sains dan Aplikasi Vol 16, No 2 (2013): Volume 16 Issue 2 Year 2013
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Penelitian tentang studi filogeni dan uji potensi antibakteri serta enzim ekstraseluler isolat Geobacillus sp. dari sumber air panas gedongsongo telah dilakukan. Penelitian ini bertujuan untuk menelusuri potensi Geobacillus sp. melalui konstruksi pohon filogeni serta mengetahui potensi-potensi molekulernya meliputi potensi antibakteri dan enzim ekstraselular xilanase dan selulase. Konstruksi pohon filogeni dilakukan menggunakan program Phylip 3.68 ed, metode Distance matriks (Neighbour joining) dengan parameter F48. Selain itu juga diuji potensi antibakteri dari metabolit sekunder Geobacillus sp. dYTae-14 dan dGae-3 dengan metode difusi perforasi terhadap Escherichia coli dan Bacillus subtilis. Uji enzim ekstraseluler xilanase dan selulase dilakukan secara kualitatif. Hasil konstruksi pohon filogeni menunjukkan bahwa kedelapan strain ini merupakan Geobacillus sp. khas Gedongsongo yang hubungan kekerabatannya sangat dekat satu sama lain dan mempunyai hubungan kekerabatan dengan bakteri Bacillus caldotenax strain BCRC 11956, Geobacillus kaustophilus subsp. Vulgivagus strain CDZ 1 dan Geobacillus thermoleovorans sp. Isolat Geobacillus sp. dYTae-14 dan dGae-3 yang diuji potensi antibakterinya tidak menunjukkan adanya potensi antibakteri terhadap Escherichia coli dan Bacillus subtilis. Kedelapan isolat Geobacillus sp. tidak menunjukkan potensi adanya enzim ekstraselluler xilanase dan selulase.

Synthesis of 4-Hydroxy-2-Methylchalcone from meta-Cresol Formilation Product and Its Activities as an Antibacteria Ismiyarto Ismiyarto; Suyanti Suyanti; Ngadiwiyana Ngadiwiyana; Purbowatiningrum Ria Sarjono; Nor Basid Adiwibawa Prasetya
Jurnal Kimia Sains dan Aplikasi Vol 21, No 4 (2018): volume 21 Issue 4 Year 2018
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Research on the synthesis of 4-hydroxy-2-methylchalcone from 4-hidroksi-2-metilbenzaldehida as formilated meta-cresol product and its antibacterial activity test has been conducted. As the first step, synthesis of 4-hydroxy-2-methylbenzaldehyde was carried out by treatment meta-cresol with chloroform through the Reimer-Tiemann formylation. Product is a brown solid with 23.94% yields. Furthermore, the 4-hydroxy-2-methylchalcone was synthesized using the 4-hydroxy-2-methylbenzaldehyde and acetophenone through the Claisen-Schmidt reaction. The product is a brownish yellow solid with 29.74% yields. Antibacterial test against Escherichia coli and Staphylococcus aureus bacteria was carried out by comparing the activity of 4-hydroxy-2-methylchalcone with 4-hydroxy-3-methoxychalcone, and chalcone compounds. The antibacterial activity test showed that the 4-hydroxy-2-methylchalcone compound, 4-hydroxy-3-methoxychalcone, and chalcone compounds gave a better antibacterial activity against Escherichia coli than the Staphylococcus aureus.

Isolasi dan Karakterisasi Enzim Xilanase dari Bacillus Subtilis pada Media Nutrient Broth dengan Penambahan Xilan Hasil Isolasi Jerami Padi Yanidya Tanjihah Ardiansyah; Nies Suci Mulyani; Purbowatiningrum Ria Sarjono
Jurnal Kimia Sains dan Aplikasi Vol 17, No 3 (2014): Volume 17 Issue 3 Year 2014
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Jerami padi merupakan limbah lignoselulosa yang mengandung xilan. Xilan pada jerami padi dapat dimanfaatkan sebagai sumber karbon, substrat dan inducer pada media pertumbuhan Bacillus subtilis untuk menghasilkan xilanase yang memiliki kemampuan menghidrolisis xilan menjadi xilosa atau xilooligosakarida. Pada penelitian ini dilakukan beberapa tahap, yaitu isolasi xilan dari jerami padi, penentuan kurva pertumbuhan Bacillus subtilis, produksi xilanase, pemurnian xilanase dengan fraksinasi bertingkat amonium sulfat dan dialisis. Pengujian aktivitas xilanase dilakukan dengan menggunakan metode DNS, sedangkan kadar protein diuji dengan menggunakan metode Lowry. Aktivitas spesifik xilanase dihitung berdasarkan unit aktivitas xilanase per mg protein. Selanjutnya dilakukan karakterisasi pada fraksi dengan aktivitas spesifik tertinggi meliputi suhu, pH dan waktu inkubasi. Hasil menunjukkan bahwa aktivitas spesifik tertinggi diperoleh pada fraksi F2 yaitu sebesar 5,178 Unit/mg protein. Kondisi optimum xilanase yang diperoleh dari hasil karakterisasi yaitu pada suhu 40°C, pH 8, waktu inkubasi 30 menit dan aktivitas spesifik fraksi F2 pada kondisi optimum sebesar 6,088 Unit/mg protein.

Co-Authors Afiina Putri Monita Agus Subagio Agustina L. N. Aminin Agustina L.N. Aminin Annisa Nur Aini Annisa Rachma Asy'ari, Mukhammad Asy’ari, Mukhammad Aulia Anggraeni Aulia Anggraeni Budi Putri Ayu Choirunnisa, Nur Fadilla Damar Nurwahyu Bima Desy Fitrya Syamsumir Dewi Kusrini Dewi Nuritasari Dini Kurnia Wisatya Dwi Hudiyanti Dwi Susilo Dyah Iswantini Dzahabiyyah, Hana Putri Enny Fachriyah Enny Fachriyah Enny Fachriyah Enny Fachriyah Enny Fachriyah Enny Fachriyah Hafizdah Fadillah Hanum, Rahma Farida Hasim - Hendra Dwipa Rifky Mahardika Huda, Muhammad Badrul Ifan Bagus Haryanto Ifsantin Nihaya Ina Noprastika Ismiyarta Ismiyarta Ismiyarto Ismitarto Ismiyarto Ismiyarto Ismiyarto Ismiyarto Ismiyarto Ismiyarto Ismiyarto Ismiyarto Ismiyarto Ismiyarto Ismiyarto Ismiyarto Khikmah Khikmah Kunti Nadiyatan S. Muthia Laksmitasari, Nuraini Dwi Liswinda Zafirah Rahmatia Lolyta Sari Br Tampubolon Mahreta Suhartanti Marcelinus Christwardana Maulida Indriyaning Ratri Melly Wahyuningsih Moch Syaiful Alam Moh. Mulyadi Mohamad Syahmie Samsudin Mukhammad Asy’ari Mukhammad Asy’ari Mumtazati, Qonita Ngadiwiyana M.Si. S.Si. Ngadiwiyana Ngadiwiyana Ngadiwiyana Ngadiwiyana Ngadiwiyana Ngadiwiyana Nies S Mulyani Nies S. Mulyani Nies Suci Mulyani Nies Suci Mulyani Niken Windi Saputri Noor Basid Adiwibawa Prasetya Nor Basid Adiwibawa Prasetya Nor Basid Adiwibawa Prasetya Nor Basid Adiwibawa Prasetya Nor Basid Adiwibawa Prasetya Nor Basid AP Nor Basyid A. Prasetya Nur Amaliyah Okky Triana Pandelaki, Elmi Christi Julia Rahmad Budiharjo Rinaryadi, Kemilau Permata Hati Salsabila, Salsabila Sandi Sutopo Aribowo Sesika Novari Sofiana Nur Aziiza Sulasiyah Sulasiyah Sumardjo, Damin Sumariyah Sumariyah Suyanti Suyanti Syahnindita Dyah Ajeng Wartari Triwijayanti, Yunita Wati, Mei Riska Wirnia S Setyani Wirnia Sinar Setyani Wuryanti Wuryanti Wuryanti Wuryanti Yanidya Tanjihah Ardiansyah Yosie Andriani Yosie Andriani Yuga Pratama

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