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All Journal Research Journal of Life Science Journal of Tropical Plant Protection AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia AGROMIX Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Agro Bali: Agricultural Journal Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Agroradix : Jurnal Ilmu Pertanian Jurnal HPT (Hama Penyakit Tumbuhan)
Mintarto Martosudiro
Departemen Hama Dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Journal : Research Journal of Life Science

 1 
Analysis of Chitinase Enzyme Trichoderma sp. in Degrading Fusarium oxysporum Rosyida, Risya; Martosudiro, Mintarto; Muhibuddin, Anton
Research Journal of Life Science Vol 9, No 3 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.03.5

Abstract

The chitinase enzyme in Trichoderma sp. play an important role in pathogen control. This is because chitin is the main component of the fungal cell wall which the chitinase enzyme can degrade. One Trichoderma sp. isolate was obtained (UBPK6) with the highest percentage of inhibition against Fusarium oxysporum, 76.71%. In addition, UBPK6 isolates also showed the highest bromocresol purple reagent test results (indicating the presence of chitinase enzymes). Chitinase enzyme activity was measured for Trichoderma asperellum isolates. The results showed that the optimum incubation period for enzyme production was the 4th day, with an enzyme activity value of 4.05 U/mL. It indicates that this time is the right time for harvesting enzymes. Furthermore, the effect of pH on the chitinase enzyme activity of Trichoderma asperellum fungus. The test results showed that the optimum value was produced at pH 5 with a value of 3.4 U/mL and decreased afterward. The pH five treatment was the best in inhibiting the growth of pathogens, with an inhibition value of 60.63%. The higher the content of the chitinase enzyme, the higher its ability to degrade damage to the germination of pathogenic spores, thus causing the growth of the pathogen to be inhibited.
Induction Resistance in Chili (Capsicum frutescens L.) to the Geminivirus Disease by Pseudomonas fluorescens Saidi, Aluna Uthilma; Martosudiro, Mintarto; Abadi, Abdul Latief
Research Journal of Life Science Vol 9, No 2 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.02.1

Abstract

This study included resistance induced by Pseudomonas fluorescens PGPR in chili (Capsicum frutescens L.) infected with Geminivirus. Geminivirus often attacks chili plants, causing disease with a yellowish color to the leaves, lobes, and stunting. Chili plants that have been attacked by the virus will experience crop failure and plant death. Virus prevention can be done with the application of PGPR by Pseudomonas fluorescens. The method used was 6 treatments on chili plants which were repeated 4 times. The analysis of variance was processed using R software. The results showed that cayenne pepper plants that were given the PGPR application had a shorter incubation period against Geminivirus attacks. Each concentration of Pseudomonas fluorescens has a mean incubation period that is not significantly different. The results also showed that there was a significant difference in the intensity of the disease that attacked the cayenne pepper plant. The application of 107 Pseudomonas fluorescens showed the most optimal results for phenol content, plant height, and number of plant leaves. The application of 109 Pseudomonas fluorescens showed optimal results for the content of IAA and the content of the peroxidase enzyme.
Fourier Transform Infrared (FTIR) Spectroscopy Method for Fusarium solani Characterization Hasanah, Ifa Maulidah; Martosudiro, Mintarto; Aini, Luqman Qurata; Prillianti, Kestrilia Rega; Prabowo, Matheus Randy
Research Journal of Life Science Vol 9, No 1 (2022)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2022.009.01.3

Abstract

The detection and identification of microorganisms using spectroscopy techniques promise to be of great value because of their sensitivity, rapidity, low expense, and simplicity.  In this study, we used FTIR spectroscopy for the characterization of Fusarium solani. PCR amplification of DNA extracted from these isolates showed the possibility of amplifying PCR products with sizes 559 bp using the ITS1-ITS4 primers. Based on phylogenetic tree analysis, the isolate of F. solani showed a closely relationship to Fusarium solani isolate MN (MH300495.1) with 99.63% similarity.  The study is focused on the carbohydrate structure which can be analyzed in the range of 900 to 1200 cm-1 of FTIR wavenumber.  The spectra of our samples share similarities with one another, although small differences occur in the absorbance value. The band at 1027 cm-1 is assigned to the C-O stretching of glycogen. Meanwhile, at 1042 cm-1 is interpreted as carbohydrate C-O stretching as well. The band around 1073 cm-1 might arise from both chitin C-C stretching and phosphate stretching of nucleic acids. Other vibrations associated with chitin are also found at 1115 cm-1 and 1151 cm-1 which are assigned to C-O-C symmetric stretching and C-O-C asymmetric stretching, respectively.
Co-Authors Abdul Choliq, Fery Abdul Latief Abadi Ajeng Wulandari Ali Pramono Alifa, Desy Hawania Asha Amanda Yayu Natasya Amanda Yayu Natasya Aminudin Afandhi Aminudin Afandhi Anna Sartika Hutapea Anton Muhibbudin Anton Muhibuddin Anugerah Firmansyah Zakaria Anugerah Firmansyah Zakaria Ari Kristini Aritonang, Awal Josua Athifa Lithiflika K. Yahya Aviva Aviolita Parama Putri Aviva Aviolita Parama Putri Aviolita Parama Putri Aviva Aviolita Parama Putri Aviolita Parama Putri Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Devi Febriana Putri Devi Febriana Putri Devy Intan Arlita Devy Intan Arlita Dian Eka Kusumawati Effendi, Mokhtar Eka Putri Irma Nuviani Ernawati, Feny Esti Yuliastri Sa’idah Esti Yuliastri Sa’idah Fanhash Nijami, Muhammad Ferry Abdul Choliq Fery Abdul Choliq Fiqh Thamrotul Irsyadah Fiqh Thamrotul Irsyadah Firdausi, Wita Hagus Tarno Haniatur Rochifah Hasanah, Ifa Maulidah Hesty Maranticha I Ketut Suada istiqomah istiqomah Istiqomah, Istiqomah Jayanti Elok Cayah Ambarwati Jayanti Elok Cayah Ambarwati Kamila Qurota A’yun Kamila Qurota A’yun Kestrilia Rega Prilianti Liliek Sulistyowati Lilik Koesmihartono Putra Lilik Nur Kholidah Lilik Nur Kholidah Lilya Echa Febriyanti Luqman Qurata Aini Luqman Qurota Aini M. Guruh Arif Zulfahmi M. Guruh Arif Zulfahmi Mahfud, Muhammad Afif Miko Putro Hutomo Miko Putro Hutomo Muhamad Ihsal Mahendra Nadia Agung Triwibawa Nadia Agung Triwibawa Nofia, Nita Nurwijayanti Prabowo, Matheus Randy Qurrota Ayuni Apriliana Rahayu, Esti Dwi Rasyada, Nuris Sa’adah Rois Hanadyo Rois Hanadyo Roswita Nur Kumalasari Rosyida, Risya Safira Candra Jalaweni Safitri, Helvira Agita Saidi, Aluna Uthilma Salsabilla, Alyara Oktavia Samsul Huda Asrori Sinta Asa Karsalina Sofyaningrum, Alaida Nur Laili Sunarto, Bintar Probo Syamsuddin Djauhari Tashya Angelique Martinez Tutung Hadi Astono Tutung Hadiastono Tutung Hadiastono Tutung Hadiastono Tutung Hadiastono Ulil Azmi Ulil Azmi Uswatun Hasanah Uswatun Hasanah Vivi Tri Kristyaningrum Yayang Cahyaning Bulan Yayang Cahyaning Bulan Yayuk Yuliati Yugista, Serdinia Zulverdi, Yudhika Ankenkuala Ramadhana Zulverdi, Yudhika Tsabitah Ramacendani