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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Bumi Lestari Ecotrophic, Journal of Environmental Science Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology SIMBIOSIS Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Jurnal Ilmiah Sains Jurnal Wilayah, Kota dan Lingkungan Berkelanjutan

I Made Sara Wijana

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 371977

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Physics Public Health Transportation

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COFFEE DECREASED THE ACTIVITY OF ALT AND AST BLOOD SERUM OF ALCOHOLIC WISTAR RATS I Made Sara Wijana; I Gede Suranjaya; Iriani Setyawati
SIMBIOSIS Vol 6 No 2 (2018)
Publisher : Jurusan Biologi Universitas Udayana

Ethanol can damage the liver, indicated by an increase of alanin amino transaminase (ALT) activity and aspartat amino transminase (AST) activity in the blood of alcohol-treated rats. Coffee is rich in antioxidants which are hepatoprotector agent. This study aimed to determine the effect of coffee to reduce alanin amino transaminase (ALT) activity and aspartat amino transminase (AST) activity in blood serum of alcohol-treated rats. The study used 32 male wistar albino rats (200±20g weight) which were divided into four treatment groups i.e. P0 (negative control which were treated with 1 mL of 30% ethanol/200g body weight (bw)/day), P1 (1 mL of 30% ethanol/200 g bw/day + coffee 0,1g/200g bw in 3,5 mL of water/200 g bw/day), P2 (1 mL of 30% ethanol/200g bw + coffee 0,15 g/200g bw in 3,5 mL of water/200g bw/day), dan P3 (1 mL of 30% ethanol/200g bw + coffee 0,2g/200g bw in 3,5 mL of water/200g bw/day). ALT and AST levels were measured by spectrophotometric method. Data analysis was done by ANOVA followed with LSD test and correlation analysis. The results showed that coffee reduced (P<0.01) the activities of ALT and AST of rats blood serum. ALT and AST activities were significantly different (P<0.01) among treatments and with control. Dose of coffee was negatively correlated with ALT activity with correlation coefficient (R) -0.978 and AST with correlation coefficient (R) -0.985 (P<0,01). The lowest of ALT and AST activities were found at dose of coffee 0.2 g/200g body weight. Keywords: ethanol, ALT, AST, coffee

DETEKSI Escherichia coli DAN ANGKA LEMPENG TOTAL BAKTERI PADA LAWAR BABI DI KECAMATAN ABIANSEMAL, KABUPATEN BADUNG, PROVINSI BALI Ni Ketut Febri Antini; Retno Kawuri; I Made Sara Wijana
SIMBIOSIS Vol 10 No 1 (2022)
Publisher : Jurusan Biologi Universitas Udayana

ABSTRACT Lawar babi is a Balinese food that is much liked, both by the locals and by tourists visiting Bali. Good and healthy food must be free of pathogenic bacteria and must meet the maximum threshold for bacterial contamination according to the Badan Pengawas Obat dan Makanan (BPOM). The purpose of this research was to determine the presence of Escherichia coli and the total plate count (TPC) of bacteria in lawar putih and lawar merah sold in Abiansemal District, Badung Regency, Bali Province. Samples were taken from 10 random traders in the Abiansemal area. This research was conducted at the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, Udayana University. Methods used in this research consists of the plating method. The test results showed that 40% of lawar merah had E. coli contamination exceeding BPOM requirements is < 3 MPN/g, while 30% of lawar putih had E. coli contamination. The highest E. coli contamination occurred in Sangeh, namely lawar merah (6.3 ± 0.76) MPN/g, while lawar putih (4.0 ± 0.49) MPN/g fluctuated. E. coli bacteria contamination in blood was highest in Sangeh at (1.9 ± 0.50) MPN/g. The results of ALT bacteria on lawar merah showed 80% did not meet BPOM requirements is < 1 x 106 CFU/g, while lawar putih did not meet BPOM requirements by 40%. The highest ALT value in pig blood in Sibang Gede II (Banjar Badung) was (18.97 ± 0.42) x 103 CFU/g. The use of fresh blood as a red dye caused an increase in bacterial contamination of lawar merah.

PENGARUH VITAMIN C PADA PROFIL DARAH TIKUS (Rattus norvegicus) JANTAN YANG DIINDUKSI NATRIUM NITRIT (NaNO2) Galih Anindita Kusumatjahja; Inna Narayani; I Made Sara Wijana
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Sodium nitrite (NaNO2) is a chemical compound that is often used in food industry. NaNO2 widely used for food preservatives, mainly in processed meat products. However, NaNO2 could cause anemia by interfering with the process of transporting oxygen in the blood. The effects caused by NaNO2 could be repaired with the use of vitamin C. This study was aimed to determine the effect of vitamin C on the blood profile of rats induced by NaNO2, and the effective dose to improve the blood profile of rats. The study used a completely randomized design (CRD) with 4 groups, namely K- (Aquades); K+ (NaNO2 37,5mg/kgBW); C1 (NaNO2 37,5 mg/kgBW + vitamin C 1,8 mg/animal/day); C2 (NaNO2 37,5 mg/kgBW + vitamin C 3,6 mg/animal/day). The samples used in each treatment group were 6 rats, therefore the total sample used was 24 male white rats (Rattus norvegicus) strain Wistar. NaNO2 and vitamin C solutions were administered orally by the gavage method. NaNO2 solution was given until 35th day, then vitamin C solution was given on 22nd to 35th day. Blood samples were taken three times, the day before the administration of NaNO2, on the 21st day, and on the 36th day, further analysis of blood used a haematology analyzer and blood smears preparations Giemsa staining. Data were statistical analyzed by ANOVA and continued with Duncan's test. The results showed that Vitamin C can improve the blood profile, with the most effective dose of vitamin C is 1,8 mg/animal/day.

Co-Authors A.A.S. A. Sukmaningsih Abd. Rahman As-syakur Adi Ariyanto Wibisono Bernard A.R Mendes Bram Setyadji Dewa Ayu Intan Tirta Sari Dhana J, Ida Bagus Galih Anindita Kusumatjahja Gede Surya Indrawan Gussasta Levi Arnenda I Gusti Ayu Kunti Sri Panca Dewi I Gusti Ngurah Kade Mahardika I Ketut Junitha I Made Saka Wijaya I Made Yunarta I Putu Sugiana I W BUDIARSA SUYASA I Wayan Suarna Ida Ayu Putu Sugiantari Ida Bagus Gede Darmayasa Inna Narayani IRIANI SETYAWATI Khotima Dwi Cahya Komang Kartika Indi Swari Lalu Muhammad Sakti Surya Jagat M. Rheza Rizki Syahputra M. Sudiana Mahendra Made Ayu Pratiwi Ngurah Intan Wiratmini Ni Ketut Febri Antini Ni Made Ernawati, Ni Made Putu Angga Wiradana Retno Kawuri Suranjaya I .Gd Viryanando Evan Rahardja

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