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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Bumi Lestari Ecotrophic, Journal of Environmental Science Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Jurnal Ilmiah Sains Jurnal Wilayah, Kota dan Lingkungan Berkelanjutan
I Made Sara Wijana
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Physics Public Health Transportation

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IDENTIFIKASI ALEL DAN KEKUATAN PEMBEDA EMPAT LOKUS DNA MIKROSATELIT KROMOSOM-Y PADA MASYARAKAT KLAN PANDE DI BALI UNTUK KEPENTINGAN FORENSIK I Ketut Junitha; I Made Sara Wijana
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 7 (2017): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2017.v07.i01.p01

Abstract

This research was conducted to know alleles variation and power of discrimination of four pair of primer: DYS19, DYS390, DYS393 and DYS395 of male Pande clan.  Epithelial cell mucus was collected  from  59 male of Gainer, Klungkung, Badung regencies and Denpasar city. Phenol chloroform method was used to extraction DNA from epithel cells sample.  Four primer pair was used to amplify DNA samples in PCR machine on 52-55oC annealing temperature. Amplicon  were running on PAGE 10% and visualized  with silver nitrate staining.  The DNA typing were conducted  to determinate of alleles size of amplicon  with plotting migration distance of amplicon on semi-log pepper.  Genetic diversity and power of discrimination was calculated used Microsoft Exell program.  The result of this research showed that 29 alleles were found range of 6-9 with mean 7.25 per locus. The genetic diversity is high category (0.739 ± 0.003, the highest diversity on DYS 390 locus  is 0.809 ±  0,004 followed by DYS395, DYS393 are 0,793 ±  0.004 and 0,720 ± 0.005 and the small one is 0.633 ±  0.003 on DYS19 locus. The power of discrimination (PD) of all loci are high category with average value is 0.892, because of / for this reason  all of loci usfulnes for  forensic purpose on pande clan in Bali.
Kajian Struktur Histologi Hati, Insang dan Lambung Ikan Nila (Oreochromis niloticus) di Danau Batur, Bangli Ida Ayu Putu Sugiantari; A. A. S. A. Sukmaningsih; I Made Sara Wijana
Buletin Anatomi dan Fisiologi Volume 7, Nomor 1, Tahun 2022
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.7.1.2022.51-59

Abstract

Danau Batur saat ini sudah mulai tercemar akibat tingginya beban pencemaran yang masuk ke perairan disebabkan oleh aktivitas masyarakat di sekitar danau. Penelitian ini bertujuan untuk melihat kerusaka struktur histologi hati, insang, dan lambung, ikan nila serta mengetahui jenis logam berat yang terdapat di perairan Danau Batur. Penelitian ini menggunakan 5 titik lokasi pengambilan sampel ikan nila. Sampel dibuat sayatan histologi organ dengan metode embedding dan analisis logam berat dengan Atomic Absorption Spectrophotometer. Hasil skoring pada insang luar, diperoleh kejadian patologi berturut-turut hiperplasia, edema lamela sekunder, dan fusi lamela paling berat 25% pada 4 lokasi, 18,7% pada lokasi 5, dan 25,00% pada lokasi 2. Pada insang dalam menunjukkan berturut-turut hiperplasia, edema lamela sekunder dan fusi lamela paling berat dengan frekuensi masing-masing 25,00% pada 4 lokasi, 25,00% pada lokasi 5, dan 25,00% pada lokasi 4. Lambung ditemukan kerusakan berupasel kariolisis, infiltrasi sel radang, dan desquamasi epitel. Histologi hati menunjukkan adanya kariolisis, karioreksis, dan degenerasi melemak. Konsentrasi logam berat di organ hati terdeteksi Fe dengan konsentrasi paling besar 3,96 ppm pada lokasi 2 dan Cu dengan konsentrasi paling besar 0,7 ppm pada lokasi 5. Ditemukannya kerusakan organ serta adanya logam berat, menandakan bahwa perairan Danau Batur mengalami pencemaran oleh aktivitas manusia di sekitar danau. Lake Batur is currently starting to be polluted due to the high pollution load enters into waters which caused of the people activities around the lake. This study aims to observe the histological structure damage of the liver, gills, and stomach, on the tilapia fishes, as well as to determine heavy metal types found in the Batur Lake water. This study was carried out 5 points of Batur Lake. The fish samples were prepared histological section by using the embedding method. Heavy metals were determined by using the Atomic Absorption Spectrophotometer. The scoring on the external gills showed there were pathological occurrences of hyperplasia, secondary lamellae edema, and lamellae fusion with most severe frequency of 25% at 4 locations, 18.7% at location 5, and 20% at location 2 respectively. Internal gills showed hyperplasia, secondary lamellae edema and most severe lamella fusion with most severe frequency of 25% at 4 sites, 25% at location 5, 25% at location 4 respectively. The stomach damaged were karyolytic cells, inflammatory cell infiltration, and epithelial desquamation. Liver histology showed karyolysis, karyorrhexis, and fatty degeneration. The heavy metals Fe and Cu were detected in the liver with Fe with the highest concentration of 3.96 ppm (Fe) at location 2 and 0.7 ppm (Cu) at location 5. The discovery of organ damages and the presence of heavy metals on the tilapia fish body indicated that Batur Lake water has been polluted by human activity around the lake. 
PROFIL BEBERAPA KOMUNITAS PERAIRAN TELUK BANYUWEDANG I Made Sara Wijana; Ni Made Ernawati
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 16 No 1 (2022)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/EJES.2022.v16.i01.p10

Abstract

Banyuwedang Bay is located in the West Bali National Park (TNBB), which is part of the conservation area. There were a lot of tourism activities developed in this area, which potentially caused disruption of the surrounding water conditions. This study aims to determine the profile of various communities in the waters of Banyuwedang Bay and as initial data to see the development of these communities due to the environmental impacts of various activities in the waters and coastal area of Banyuwedang Bay. Data was collected from five observation stations including: Community structure of Plankton, Macrozoobenthos, and seagrass, and coral reef cover. Result shows that the phytoplankton community consist of 9 genera where Nitzschia sp. (Bacillariophyceae) as the dominant species found. Phytoplankton have low diversity index ranging between 0.348 - 1.411. Zooplankton diversity index also has low value 0.693 with three genera found namely Copepoda sp., Nauplius sp. and Daphnia sp. Macrozoobenthos community is composed of 17 macrozoobenthos genera, with low diversity index ranging ??between 0.562-1.809. There are only two species of seagrass, Halophila ovalis and Cymodocea rotundata, which were found in Banyuwedang Bay. Coral reef cover seems to be ranged from poor to good conditions, with 25% - 73% coverage. Based on the result, we concluded that the Banyuwedang Bay has a moderate condition to the growth of plankton and seagrass, but good for coral reefs. Periodic monitoring is needed to find out changes in conditions that can occur in Banyuwedang Bay. Keywords: Banyuwedang Bay; community structure; macrozoobenthos
Struktur dan Komposisi Vegetasi pada Suksesi di Muara Sungai Unda, Kabupaten Klungkung, Bali I Made Saka Wijaya; Gede Surya Indrawan; Putu Angga Wiradana; I Made Sara Wijana; Abd. Rahman As-syakur; Adi Ariyanto Wibisono; Viryanando Evan Rahardja
JURNAL ILMIAH SAINS Volume 21 Nomor 1, April 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1094.862 KB) | DOI: 10.35799/jis.21.1.2021.31744

Abstract

 Letusan Gunung Agung pada tahun 1963 memberikan dampak ekologis di sekitar muara Sungai Unda, Kabupaten Klungkung, Bali. Lahar yang mengalir melalui sungai tersebut menyebabkan terjadinya suksesi primer akibat tutupan pasir dan batu. Selama 57 tahun, muara Sungai Unda tidak hanya mengalami suksesi alam, tetapi juga mengalami suksesi antropogenik melalui pemanfaatan sebagai tambang pasir, lahan pastura, dan permukiman sementara.  Penelitian ini bertujuan untuk mempelajari struktur dan komposisi vegetasi pada suksesi di muara Sungai Unda. Penentuan struktur vegetasi dilakukan pada 8 stasiun penelitian dengan menggunakan metode plot dengan jumlah 24 plot pohon (20 x 20 m) dan 48 plot vegetasi lantai (1 x 1 m). Data dianalisis menggunakan parameter ekologis seperti densitas, densitas relatif, frekuensi, frekuensi relatif, dominansi, dominansi relatif, dan nilai penting, serta dilengkapi dengan indeks keragaman Shannon-Weiner (H’), indeks dominansi (C), dan indeks keseragaman (E). Hasil penelitian menunjukkan bahwa semak dan rumput merupakan bentuk hidup yang paling menentukan struktur vegetasi di Muara Sungai Unda. Beberapa spesies penyusun vegetasi tersebut merupakan tumbuhan invasif seperti yang harus dikontrol populasinya adalah Typha angustifolia, Ipomoea carnea, Chromolaena odorata, Lantana camara, dan Vachellia farnesiana. Berdasarkan indeks yang digunakan, keanekaragaman vegetasi termasuk dalam kategori tinggi, tanpa ada jenis yang mendominasi, dengan persebaran yang merata.Kata kunci: Gunung Agung; spesies invasive; suksesi antropogenik; vegetasi suksesiStructure and Composition of Successional Vegetation in Unda River, Klungkung Regency, BaliABSTRACTMount Agung eruption in 1963 caused major ecological impact around Unda River, Klungkung Regency, Bali. The volcanic mudflow that surpass the Unda River watershed initiate the primary succession in those areas. This research aimed to study the structure and composition of successional vegetation in Unda River. Twenty-four tree plots (20 x 20 m) and 48 understorey plots (1 x 1 m) derived from 8 research stations were used to determine the structure of vegetation. The data analyzed by ecological parameters, such as density, relative density, frequency, relative frequency, dominancy, relative dominancy, and importance value, then completed with Shannon-Weiner diversity index (H’), dominancy index (C), and evenness index (E). The results shown that the vegetation structure in Unda River determined by two major growth form: shrubs and grasses. Some notable invasive species that should be controlled are Typha angustifolia, Ipomoea carnea, Chromolaena odorata, Lantana camara, and Vachellia farnesiana. Based on the three indexes, the vegetation diversity was categorized as high diversity, without any species domination, and the distribution of the species are uniformKeywords: Anthropogenic succession; invasive species; Mount Agung; successional vegetation
Potensi Bacillus sp. Sebagai Agen Antagonis Terhadap Athelia rolfsii Penyebab Busuk Pangkal Batang Kedelai (Glycine max L.) Khotima Dwi Cahya; Retno Kawuri; I Made Sara Wijana
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p12

Abstract

Athelia rolfsii dapat menyebabkan busuk batang pada tanaman kedelai dengan gejala infeksi pada pangkal batang yang berbatasan dengan permukaan tanah yang dapat menurunkan hasil produksi panen kedelai hingga 75%. Pengendalian penyakit dengan pestisida kimia diketahui dapat membawa dampak negatif tehadap lingkungan. Bakteri Bacillus sp. sebagai agen antagonis diketahui dapat menghasilkan enzim, antibiotik, dan siderophore yang mampu menekan pertumbuhan cendawan patogen. Penelitian ini bertujuan untuk mengetahui kemampuan Bacillus sp. yang diisolasi dari rhizosfer tanaman kacang tanah sehat dalam menekan pertumbuhan cendawan A. rolfsii secara in vitro dan untuk mengetahui konsentrasi kultur Bacillus sp. terbaik dalam menekan pertumbuhan cendawan A. rolfsii dalam skala green house. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan parameter yang diamati adalah persentase daya hambat secara in vitro, persentase kejadian penyakit, dan persentase intensitas penyakit. Data dianalisis dengan analisis sidik ragam (ANOVA). Hasil penelitian diperoleh 6 isolat Bacillus sp. Isolat Bacillus sp. 1 sebagai isolat dengan persentase daya hambat terbaik dalam menekan pertumbuhan A. rolfsii (79,44%) selanjutnya diuji pada skala green house. Pemberian kultur Bacillus sp.1 sebanyak 10 mL dengan kerapatan 1x108 sel/mL dapat menekan kejadian penyakit busuk pangkal batang hingga 53,38 ± 18,23% dan menekan persentase intensitas penyakit hingga 67,78 ± 19,40% selama 7 HST. Kontrol positif dengan Amistar top menekan kejadian penyakit busuk pangkal batang dan intensitas penyakit hingga 86,68 ± 18,25%. Kultur Bacillus sp. 1 dapat digunakan sebagai agen antagonis pada penyakit busuk pangkal batang yang disebabkan oleh A. rolfsii. Kata kunci: Antagonis, Athelia rolfsii, Bacillus sp., Tanaman kedelai.
INDEKS STRUKTUR KOMUNITAS DAN SAPROBITAS PLANKTON SERTA CHECKLIST KEBERADAAN NEKTON DI PERAIRAN DANAU BERATAN, BALI Gede Surya Indrawan; I Made Sara Wijana; I Made Saka Wijaya; Abd. Rahman As-syakur; I Putu Sugiana; I Made Yunarta
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 16 No 2 (2022)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/EJES.2022.v16.i02.p10

Abstract

Beratan Lake was mainly popular for tourism and aquaculture activities that affecting water's environmental characteristics. The water healthiness indicator can be seen through the organism's existence, namely plankton to nekton biota that can be uses as an essential parameter in monitoring water conditions. This study aims to determine the plankton community structure index, saprobity index and the presence of nektons in the Beratan Lake. Plankton were sampled using the pouring method while the nekton sample was taken randomly through direct sampling with fishing nets and conducted interviews with the surrounding local community and fishermen. The results of the study found that the abundance of phytoplankton was dominated by Staurastrum with abundance level 1,836.92 ind/L. Meanwhile, the zooplankton was dominated by Rotifera with abundance level 8.17 ind/L. The diversity index, uniformity and dominance of phytoplankton are 1.31, 0.60 and 0.33 respectively, while the saprobity index is 1.08 which classifies the waters of Beratan Lake as being in a lightly polluted condition. There were 18 species of nektons in Beratan Lake were found, with one endemic species Rasbora balinensis and 13 foreign fish. There also an invasive species, namely zebrafish (Amatitlania nigrofasciata) was found in this Lake. From the results of the study, we hope that the government in cooperation with the surrounding community, can control the ecological conditions of the waters of Beratan Lake, especially the presence of invasive species which are increasing in number, as well as protect endemic species. Keywords: Abundance; beratan lake; community structure; plankton; saprobity
EVALUASI SISTEM DAN DAMPAK LINGKUNGAN PENGELOLAAN AIR TERPRODUKSI PADA SUMUR X1 PT MGBI LAPANGAN WUNUT Dhana J, Ida Bagus; Budiarsa Suyasa, I Wayan; Sara Wijana, I Made
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 18 No 1 (2024)
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/EJES.2024.v18.i01.p02

Abstract

PT Minarak Gas Brantas Inc.'s operations in the Wunut Field involve the production of waste in various forms, primarily liquid waste, with produced water being the most significant type. Proper management of this produced water waste is crucial to prevent environmental pollution and ensure economic and environmental sustainability. The company manages this waste by reinjecting it into the reservoir to prevent groundwater contamination. To ensure the effectiveness of this reinjection process, they have created a groundwater flow pattern map (flow net) at three locations near the injection well: LP1 in Keduung Boto Village, LP2 in Candi Pari Village, and LP3 in Candi Pari Village, all in Sidoarjo Regency. This helps in understanding the potential spread of pollutants in the soil and underground water, aiding in environmental pollution monitoring and managment.The groundwater from this area is used for various human activities like bathing, cooking, and washing. However, based on water quality standards outlined in Ministry of Health Regulation No. 2 of 2023, the produced water from Well X-1 in the Wunut Field does not meet the required TDS (Total Dissolved Solids) content standards due to a decline in hydrocarbon fluid production, resulting in a separator efficiency of 74,86%. Additionally, lab tests and flow net results indicate that the TDS levels in LP1, LP2, and LP3 do not meet the sanitation and hygiene water quality standards, suggesting potential seepage in these locations that could contaminate groundwater. Keywords: Oil; Gas Produced Water; Gas Separator Eficiency; Groundwater Flow
Studi Kualitas Air, Status Mutu Air dan Beban Pencemaran Sungai Badung Bernard A.R Mendes; I Wayan Suarna; I Made Sara Wijana
JURNAL WILAYAH, KOTA DAN LINGKUNGAN BERKELANJUTAN Vol. 3 No. 2 (2024): JURNAL WILAYAH, KOTA DAN LINGKUNGAN BERKELANJUTAN
Publisher : Fakultas Teknik Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58169/jwikal.v3i2.529

Abstract

This study aims to analyze the water quality of the Badung River based on Class II River water quality standards according to Government Regulation No. 22 of 2021, determine the water quality status, and calculate the potential pollution load of the Badung River. Water quality measurements were conducted at five sampling points. The parameters measured and observed include Temperature, Total Suspended Solids (TSS), pH, Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Dissolved Oxygen (DO), Sulfate (SO42), Nitrate (NO3), Total Phosphate (P), Oil & Grease, Total Detergent, and Total Coliform. Samples were analyzed in the laboratory for physical, chemical, and microbiological parameters. The water quality status of the Badung River was determined using the pollution index method, and the potential pollution load was calculated using the wastewater emission coefficient approach. The results showed that the water quality and status of the Badung River are classified as lightly polluted, with an average pollution index value of 3.65. The potential pollution load entering the Badung River in the domestic sector is 0.78 tons/day for BOD, 1.07 tons/day for COD, and 0.74 tons/day for TSS. Factors contributing to the lightly polluted water quality status include population density, poorly managed domestic activities (household waste), which contribute to high pollution parameter values such as BOD, COD, and TSS, and a lack of waste management infrastructure
Co-Authors A.A.S. A. Sukmaningsih Abd. Rahman As-syakur Adi Ariyanto Wibisono Bernard A.R Mendes Bram Setyadji Dewa Ayu Intan Tirta Sari Dhana J, Ida Bagus Galih Anindita Kusumatjahja Gede Surya Indrawan Gussasta Levi Arnenda I Gusti Ayu Kunti Sri Panca Dewi I Gusti Ngurah Kade Mahardika I Ketut Junitha I Made Saka Wijaya I Made Yunarta I Putu Sugiana I W BUDIARSA SUYASA I Wayan Suarna Ida Ayu Putu Sugiantari Ida Bagus Gede Darmayasa Inna Narayani IRIANI SETYAWATI Khotima Dwi Cahya Komang Kartika Indi Swari Lalu Muhammad Sakti Surya Jagat M. Rheza Rizki Syahputra M. Sudiana Mahendra Made Ayu Pratiwi Ngurah Intan Wiratmini Ni Ketut Febri Antini Ni Made Ernawati, Ni Made Putu Angga Wiradana Retno Kawuri Suranjaya I .Gd Viryanando Evan Rahardja