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Effect of temperature and storage duration of Aedes aegypti mosquito specimens artificially infected with dengue-3 virus on the results of immunohistochemical examination Susilawati Sitti Rohmah Umniyati Tri Baskoro Tunggul Satoto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

To confirm the presence of any dengue viral in a mosquito, mosquito’s head can be squashed ona slide and stained with immunohistochemical staining. The remaining samples then can bestored in the cryo freezer at -80ºC to avoid specimen damage. However, for laboratories withlimited facilities, with only a refrigerator with a temperature range of -20ºC to 4ºC is available,examination to evaluate whether the dengue antigen can still be detected in specimens storedat these temperature is necessary. It was a quasi-experimental study. Three to five-day-old adultfemale Aedes aegypti mosquitoes were injected intrathoracically with dengue-3 (DENV-3) strainH-87 virus and then maintained for about 7 days. The dengue viral antigen on mosquitoes wasidentified using immunohistochemical method after stored at a temperature of 4oC, -20oC, -80oCfor 2, 4 and 8 weeks. Mosquito specimens that were not stored were used as a positive control.Kappa value was counted to analyze level of agreements between two observers. Two-wayAnova was used to analyze mean positive rates. Kappa value showed poor agreement (0.00-0.16) between two observers when the specimens were stored at 4oC for 2-8 weeks, and showedgood agreement (Kappa value of 0.77), when stored at -20oC for 4 weeks. The kappa valueshowed very good agreement (0.90-0.92) when the specimens were stored at -20oC for 2 weeks,and at -80oC for 2-8 weeks. Mean positive rates of the specimens stored at 4oC were significantlylower (p<0.005) than stored at -20oC and -80oC, but there were no significant differencesbetween specimens stored at -20oC and -80oC (p>0.05). In conclusion, availability of thedengue viral antigen on mosquito specimens was influenced by temperature and storage durationof the specimens.Keywords: dengue virus - head squash - immunohistochemistry - kappa agreement - temperature
Risk factors related to malaria incidence at Santu’un village, Tabalong District, South Kalimantan Province Junus Widjaja Supargiyono Tri Baskoro Tunggul Satoto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 43, No 02 (2011)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Santu’un Village is one of malaria endemic areas in Muara Uya Sub district, Tabalong District, South KalimantanProvince, Indonesia. Annual Parasite Incidences (APIs) from 2007 to 2009 were 11.4, 82.9, and 25.8‰ respectively.The majority of Muara Uya population are rubber tappers, gold miners and loggers in the forest. Moreover, most ofthe Muara Uya population do not use mosquito nets when sleeping at night. These occupations and communityhabits may contribute in high malaria incidence in Muara Uya. However, study concerning risk factor of malaria hasnot been performed yet. The study was conducted in order to evaluate risk factors that might correlate with malariaincidence in Santu’un village, Muara Uya subdistrict. Two hundred and ninety one subjects were enrolled in thisstudy. Those consisted of 70 people whom microscopically malaria positive and 221 people whom malaria negative.Household visits were conducted to each subject to perform interviews. Data collection of malaria risk factors wasobtained using questionaire. The results indicated that three main occupations, loggers, gold miners and rubbertapper, were significantly represent risk factors of malaria incidence. The community habits that also significantlycorrelated with malaria incidence were the not using of mosquito repellents and not using bed nets. Two clusteringcases were also observed during the year of 2010. The coordinate of the first cluster was 1.863500 S and 115.606700E with diameter of 0.65 km and the coordinate of the second cluster was 1.838667 S and 115.607200 E withdiameter of 0.67 km. In conclusion, in Santu’un village, the risk factor that correlated with the malaria incidencewere loggers, gold miners, rubber tappers. In addition the not using of mosquito repellents and not using bed netswere also risk factors of malaria incidence.Key words: malaria -risk factors-annual parasite incidence-Muara Uya-South Kalimantan
Organophosphate insecticide susceptible test and transovarial transmission detection of dengue virus on Aedes aegypti in Kendari Muhaimin Saranani; Sitti Rahmah Umniyati; Tri Baskoro Tunggul Satoto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 45, No 04 (2013)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (968.716 KB) | DOI: 10.19106/JMedScie004504201303

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Dengue Fever (DF) is a disease caused by the dengue virus that transmitted by Aedes aegypti(Ae. Aegypti) and Ae. albopictus. Dengue fever is now one of the most important public healthproblems in Indonesia. Vector control using insecticides is the most important strategy to controlthe DF. Massal fogging and selective abatisation have implementedd intensively to control Ae.aegypti. However after its a long time implementation, mosquitoes resistance and transovarialtransmission have been reported. The aim of the study was to evaluate the susceptibility statusof Ae. aegypti to organophosphate and its ability to transovarial transmit degue virus in KendariCity, South East Sulawesi. This was a observational study using cross-sectional design conductedin high endemic areas (Kadia Village) and low endemic areas (Kambu Village). Susceptibilitystatus of Ae. aegypti larvae from F1’s egg generation was evaluated by biochemic assay, whereasthe transovarial transmission of dengue virus of adult female Ae. aegypti was evaluated byimmunohistochemistry method using head squash preparation. The results showed that the Ae.aegypti larvae resistant was higher in high endemic areas (Kadia Village) (83.33%) than in lowendemica areas (Kambu Village) (60.00%). In addition, transovarial transmission index (TTI) ofAe. aegypti dengue virus in the high endemic areas (26%) was significantly higher than in thelow endemic areas (12%) (p<0.05). In conclusion, the Ae. aegypti larvae resitance toorganophosphate as well as the TTI in high endemic areas is higher than in low endemic area inKendari, Sout East Sulawesi.
Insecticide resistance and mechanisms of aedes aegypti (Diptera: Culicidae) in Yogyakarta, Indonesia Budi Mulyaningsih; Sitti Rahmah Umniyati; Tri Baskoro Tunggul Satoto; Ajib Diptyanusa; Dwi Aris Agung Nugrahaningsih; Yahiddin Selian
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 50, No 1 (2018)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.745 KB) | DOI: 10.19106/JMedSci005001201803

Abstract

For several decades, applications of organophosphates and pyrethroids insecticides have been extensively used to control Aedes aegypti as the primary dengue vector. Hence it is important to study dengue vector resistance status and its mechanisms in relation to long term use of insecticides. This study aimed to determine the resistance status and to characterize mechanisms of Ae. aegypti to organophosphates and pyrethroids using biological, biochemical and molecular assays. Larvae and puppae of Ae. aegypti were collected in the field of Plosokuning, Minomartani, Sleman, Yogyakarta, Indonesia. The biological assay was carried out using CDC Bottle Bioassay to test the resistant status to malation and cypermetrin. The biochemical assay was conducted using microplate assay with substrate α-naphthyl acetate to test the presence of esterase elevated activity, and the molecular assay was done using PCR with AaSCF7 and AaSCR7 primer to detect of point mutation at 1534 site, that located in the area of segment 6 of domain III. The biological assay showed Ae. aegypti suggests the possibility of resistance to malathion (mortality 82%) that needs to be confirmed further and already resistant to cypermetrin (mortality 76%). The biochemical assay of Ae. aegypti showed the presence of non-specific esterase elevated activity. The PCR method showed specific DNA bands were formed with the size of 748bp, and with sequencing showed there has been F1354C point mutation of voltage gated sodium chanel gene in the area of segment 6 of domain III. Long term use of insecticides did not successfully eliminate the targeted dengue vector, because Ae. aegypti mosquitoes were resistant to both insecticides. The results demonstrate the importance of designing better health policies regarding insecticide usage
Optimization of Formula Granule of Lempuyang Gajah (Zingiber zerumbet (L) J.E.Smith) Rhizome Purified Extract as a Larvicide Tri Murini; Mae Sri Hartati Wahyuningsih; Achmad Fudholi; Tri Baskoro Tunggul Satoto
Majalah Obat Tradisional Vol 25, No 1 (2020)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.48754

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Lempuyang gajah rhizome (Zingiber zerumbet (L) Smith is considered potential as larvicidal. A previous study has shown that the purified extract of Z. zerumbet rhizome was toxic against Adese aegypti larvae.  The aim of the study it to formulate a purified extract of Z. zerumbet (L) Smith in granule preparations by combining Sodium starch glycolate, PVP K-30 and tween 80.  The granule formulation was optimized by the Simplex Lattice Design (SLD) method with using Design expert program 7.1.5. The results showed that the interaction of the three components can increase the flow rate index, the angle of repose, and reduce absorption. The optimum formula obtained was Sodium starch glycolate 2%, PVP K-30 2%, and Tween 80 5%. The analysis of one sample t-test shows that there is no significant difference between the predicted parameter values and the experimental results of the flow rate index and angle of repose, while the absorption response is significantly different. The optimum formula for granules has larvicidal activity with 100% larval death during 12-hour treatment.
Analisis Faktor Keberadaan Vektor Penular DBD Melalui Identifikasi Tempat Potensial Perkembangbiakan Nyamuk Aedes Sp. di Desa Gergunung, Kabupaten Klaten, Jawa Tengah Nur Alvira Pascawati; Ellen Petra Songjanan; Elisabeth Deta; Tri Baskoro Tunggul Satoto
Jurnal Manajemen Kesehatan Indonesia Vol 6, No 1 (2018): April 2018
Publisher : Magister Kesehatan Masyarakat, Fakultas Kesehatan Masyarakat, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmki.6.1.2018.29-38

Abstract

The presence of potential breeding places can be used to measure the risk of DHF transmission in Klaten. This result can be used by the community to eridication of mosquito breeding place on terget and can improve to early warning system through effective methods for vector control. Type of study was analytic with cross-sectional study. The study was conducted in 104 households with simple random sampling technique. Data analysis used three stages: univariate, bivariate (Chi-square, Kolmogorov Smirnov, Spearman rank) and multivariate test with α=5%. The density of larvae based on the value of CI, HI, and BI amounted to 1.6%, 19.2%, 27.9%. The potential place for larvae breed based on Maya Index of 76%. Containers of open wells with groundwater sources, the volume of water is more than 1 liter and the material from cement can be the preferred place for mosquitoes to breed. Multivariate analysis shows that open wells are strongly related and has a risk of 1,556 (CI: 1,199-2,019) increasing the density of larvae compared with other variables.The density of larvae based on BI is a high category, HI is a moderate category and CI is a low category. This condition is influenced by the presence of open wells made of cement and can hold water in large quantities. Improve to early warning system of DHF transmission through identification of the presence of potential breading places in this area are to close the wells at the household level and conduct regular monitoring on the Controllable Sites.
FLUKTUASI DEMAM BERDARAH DENGUE TERKAIT VARIABILITAS CUACA DI KLATEN, INDONESIA Tri Baskoro Tunggul Satoto; Nur Alvira Pascawati; Ajib Diptyanusa; Luthfan Lazuardi; Alvin Harjono Dwiputro; Ariyanto Nugroho
Vektora : Jurnal Vektor dan Reservoir Penyakit Vol 13 No 1 (2021): Vektora : Jurnal Vektor dan Reservoir Penyakit
Publisher : Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/vk.v13i1.4333

Abstract

Klaten Regency is one of the Dengue Hemorrhagic Fever (DHF) endemic areas in Central Java. Weather conditions can have an impact on vector dynamics, dengue virus development, and interactions between mosquitoes and humans. The purpose of this study was to determine the pattern of dengue transmission in twenty-six sub-districts in Klaten Regency based on wind speed, specific humidity, rainfall, and temperature. This study was conducted using a retrospective cohort design based on Giovanni-National Aeronautics and Space Administration (NASA) data during the last three years (2016-2018). The independent variables in this study were: wind speed (m/s), specific humidity (g/kg), rainfall (mm/month), and temperature (oC), while the dependent variable was the number of dengue cases in 26 sub-districts in 2014-2014. 2016. Data were analyzed based on monthly patterns and regional patterns using correlation and regression tests with =0.05. The results showed that a total of 1,434 dengue cases were reported during this time period. Weather data analysis revealed that DHF fluctuations were correlated with wind speed in four sub-districts, specific humidity in seven sub-districts, rainfall in three sub-districts, and temperature in three sub-districts. Specific humidity variation plays a role of 21.8% as the dominant factor that can explain the case of DHF in the Klaten Regency. The results of this study can be applied to mitigate the transmission of DHF by determining preventive actions according to place and time and increasing the early warning system to deal with the threat of DHF outbreaks. Abstrak Kabupaten Klaten adalah salah satu daerah endemis Demam Berdarah Dengue (DBD) di Jawa Tengah. Kondisi cuaca dapat berdampak pada dinamika vektor, perkembangan virus dengue, dan interaksi antara nyamuk dengan manusia. Tujuan dari penelitian ini adalah untuk mengetahui pola penularan DBD di dua puluh enam kecamatan yang berada di Kabupaten Klaten berdasarkan kecepatan angin, kelembaban spesifik, curah hujan dan suhu. Penelitian ini dilakukan menggunakan desain kohort retrospektif berdasarkan pada data Giovanni-National Aeronautics and Space Administration (NASA) selama 3 tahun terakhir (2016-2018). Variabel bebas dalam penelitian ini adalah: kecepatan angin (m/s), kelembaban spesifik (g/kg), curah hujan (mm/bulan) dan suhu (oC), sedangkan variabel terikat adalah jumlah kasus DBD di 26 kecamatan pada tahun 2014-2016. Data dianalisis berdasarkan pola bulanan dan pola wilayah dengan menggunakan uji korelasi dan regresi dengan α=0,05. Hasil penelitian menunjukkan bahwa total sebanyak 1.434 kasus dengue dilaporkan selama periode waktu tersebut. Analisis data cuaca mengungkapkan bahwa fluktuasi DBD berkorelasi dengan kecepatan angin di empat kecamatan, kelembaban spesifik di tujuh kecamatan, curah hujan di tiga kecamatan dan suhu di tiga kecamatan. Variasi kelembaban spesifik berperan sebesar 21,8% sebagai faktor dominan yang dapat menjelaskan kasus DBD di Kabupaten Klaten. Hasil studi ini dapat diaplikasikan untuk mitigasi penularan DBD dengan menentukan tidakan pencegahan menurut tempat dan waktu serta meningkatkan sistem kewaspadaan dini untuk menghadapi ancaman KLB DBD.
Pemberdayaan Masyarakat Dalam Pengendalian Demam Berdarah Dengue (Literature Review) Tri Yuni Sukesi; Supriyati Supriyati; Tribaskoro Tunggul Satoto
Jurnal Vektor Penyakit Vol 12 No 2 (2018): Edisi Desember
Publisher : Balai Penelitian dan Pengembangan Kesehatan Donggala, Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.928 KB) | DOI: 10.22435/vektorp.v12i2.294

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Abstract Dengue Haemorrhagic Fever (DHF) was still a public health problem that has been more than 20 years with various efforts. Community participation was great for reducing the incidence of DHF. Community empowerment will greatly assist the government in succeeding DHF preventive efforts so that DHF can be controlled. The method used in this study was a literature study that examines the various references closely related to community empowerment in the control of DHF diseases. References examined come from the results of research both from within or abroad, books and official reports issued within a period of not more than 10 years. The number of literature studied was 35 literatures.Community empowerment in the control of DHF was necessary because the government can not run alone in efforts to control DHF. All programs that were rolled out will be useless if the community was not involved in planning, monitoring and evaluation processes. This is because DHF was related to environmental problems in which humans were involved in creating an enabling environment for the spread of DHF Community empowerment in . DHF control was important to support the implementation and sustainability of DHF control program. Abstrak Demam Berdarah Dengue (DBD) masih merupakan masalah kesehatan masyarakatwalaupun sudah dikendalikan lebih dari 20 tahun dengan berbagai upaya. Peran sertamasyarakat sangat besar dalam upaya pengendalian sehingga pemberdayaanmasyarakat penting dilakukan untuk mengurangi kejadian penyakit DBD.Pemberdayaan masyarakat akan sangat membantu pemerintah dalam menyukseskanupaya preventif DBD sehingga DBD dapat dikendalikan. Metode yang digunakan dalamkajian ini adalah studi literatur yang mengkaji berbagai referensi yang erat kaitannyadengan pemberdayaan masyarakat dalam pengendalian penyakit DBD. Referensi yangdikaji berasal dari hasil penelitian baik dari dalam atau luar negeri, buku dan laporanresmi yang dikeluarkan dalam kurun waktu tidak lebih dari 10 tahun. Jumlah literatureyang dikaji sebanyak 35 literatur. Pemberdayaan masyarakat dalam pengendalian DBDdiperlukan karena pemerintah tidak bisa berjalan sendiri dalam upaya pengendalianDBD. Semua program yang digulirkan akan tidak berguna apabila masyarakat tidakdilibatkan dalam perencanaan, proses monitoring dan evaluasi. Hal ini disebabkankarena DBD berhubungan dengan masalah lingkungan dimana manusia terlibat dalammenciptakan lingkungan yang mendukung terhadap penyebaran penyakit DBD.Pemberdayaan masyarakat dalam pengendalian DBD penting untuk menunjangpelaksanaan dan keberlangsungan program pengendalian DBD.
Detection of Polymorphism on Voltage-gated Sodium Channel Gene of Indonesian Aedes aegypti Associated with Resistance to Pyrethroids Budi Mulyaningsih; Sitti Rahmah Umniyati; Tri Baskoro Tunggul Satoto; Ernaningsih Ernaningsih; Dwi Aris Agung Nugrahaningsih
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.425

Abstract

BACKGROUND: Aedes aegypti is a vector of several pathogens including dengue virus. Vector control is an effective way to break the transmission but unfortunately constant use of insecticides ultimately causes vector resistance. Pyrethroids have been used for about 15 years to combat Ae. aegypti in Yogyakarta Province, Indonesia. Single amino acid substitutions in the voltagegated sodium channel associated with pyrethroid resistance constitute one of the main causative factors of knockdown resistance (kdr). The aim of this study is to detect resistant status to cypermetrine and polymorphism on the voltagegated sodium channel gene of Ae. aegypti from 2 dengue endemic areas in Yogyakarta Province (Yogyakarta city and Sleman district).METHODS: Pyrethroid resistance in Ae. aegypti mosquitoes was detected by using CDC Bottle Bioassay. To detect the polymorphism on the voltage-gated sodium channel gene of Ae. aegypti analyses were conducted by using PCR and direct DNA squencing with primers AaSCF1 and AaSCR4 for S989P, I1011M (or V), L1014F sites, and AaSCF7 and AaSCR7 for the F1534C site.RESULTS: According to bioassay, the results for Ae. aegypti from Yogyakarta city (93% mortality) and Sleman district (88% mortality) suggest the possibility of resistance to cypermethrin. We observed polymorphism on voltagegated sodium channel gene on site F1534C (heterozygous).CONCLUSION: The findings provide early evidence that the use of cypermethrin (pyrethroids) in Yogyakarta city and Sleman district, Yogyakarta Province, Indonesia is reducing its effectiveness to control Ae. aegypti. Recommendations include additional tests for confirmation.KEYWORDS: Aedes aegypti, dengue virus, cypermethrin, Bioassay, Yogyakarta
HUBUNGAN TRANSOVARIAL INFECTION RATE DENGAN INCIDENCE RATE DEMAM BERDARAH DENGUE DI KELURAHAN PERKAMIL KOTA MANADO Joy Victor Imanuel Sambuaga; Tri Baskoro Tunggul Satoto
Jurnal Kesehatan Lingkungan Vol 5 No 1 (2015): JURNAL KESEHATAN LINGKUNGAN
Publisher : POLTEKKES KEMENKES MANADO

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47718/jkl.v5i1.594

Abstract

Penelitian ini bertujuan untuk mengetahui seberapa besar hubungan transovarial Infeksi Tingkat Demam Berdarah Insiden Tingkat di desa Perkamil Manado Kota pada tahun 2011. Jenis penelitian observasional (survei). Pengumpulan data dilakukan pada bulan Januari 2011 dengan menggunakan single larva dalam setiap 15 rumah di lokasi kasus DBD di Desa Perkamil pada tahun 2010. Setiap lokasi DBD dalam buffer dengan radius 100 meter dari rumah, dan kasus-kasus yang dipilih dari 15 rumah sampel. Survei menggunakan alat seperti, pipet, lampu senter dan GPS. Dari setiap rumah sampel titik koordinat diambil kemudian diperiksa larva di setiap kontainer yang berisi air di dalam dan di luar rumah. Pada setiap kontainer ditemukan larva diambil satu dengan menggunakan pipet kecil, larva dimasukkan ke dalam botol berlabel atau kode dan penentuan spesies menggunakan mikroskop. Penelitian menunjukkan bahwa ada hubungan yang signifikan antara indeks transovarial infeksi (TIR) dan kejadian kasus DBD di desa Perkamil.
Co-Authors Achmad Fudholi Ajib Diptyanusa Ajib Diptyanusa Ajib Diptyanusa Alamri, Annisa Rizqa Ali Wardana Ali Wardana Alvin Harjono Dwiputro Alvira Pascawati, Nur Angle Maria Hesti Sorisi Anies Mulyani Anis Fuad Anwar Dwi Cahyono Ardhi, Khairil Ariyanto Nugroho Barandi Sapta Widartono Barandi Sapta Widartono Budi Mulyaningsih Budi Mulyaningsih Budi Mulyaningsih Budi Mulyaningsih Damar Tri Boewono Diptyanusa, Ajib Diptyanusa, Ajib Dwi Aris Agung Nugrahaningsih Dwi Sarwani Sri Rejeki Dwiputro, Alfin Harjuno Dyah Mahendrasari Sukendra E. Elsa Herdiana Murhandarwati Edhi Martono Elisabeth Deta Lustiyati Ellen Petra Songjanan Ellen Petra Songjanan Ernaningsih Ernaningsih Ernaningsih Ernaningsih Frutos, Roger Fudholi, Achmad - Hakim, A. Ulil Fadli Hanani Melangwala Laumalay Hardaningsih, Ignatius Hari Kusnanto Hastuti Tongkodu I Kadek I Kadek Mulyawan I Made Swastika Ikhwan Mustiadi Insani, Dini Aura Joy Victor Imanuel Sambuaga Kurnia, Risman Kusumasari, Rizqiani Amalia Kuswati Kuswati Laksono, Rian Kurniawan Lilik Zuhriyah Lusiana Utari, Evrita Lutfan Lazuardi Lutfan Lazuardi Luthfan Lazuardi M Lutfan Lazuardi Mae Sri Hartati Wahyuningsih Maguin, Sylvie Mahardika Agus Wijayanti Mbiliyora, Agustina Mubarak Mubarak muhaimin saranani Mujiyanto Mujiyanto Mujiyanto Mujiyanto Mulyawan, I Kadek Najla, Aesha Pangesti, Rachma Widya Pascawati, Nur Alvira Perdana, Taufik Mulya Purwono Purwono R. Suharyadi Risdwiyanto, Rifa Nadhifa Ristiyanto Roger Frutos Roger Frutos Roger Frutos Ruben Wadu Wila Satrisno, Hary Setiawan, Yohanes Didik Siti Istianah Sitti Rahmah Umniyati Sitti Rahmah Umniyati Sitti Rahmah Umniyati Sitti Rahmah Umniyati Sitti Rahmah Umniyati Sitti Rahmah Umniyati Slamet Riyadi Suhermanto - Sulistyawati Sulistyawati Supriyati Sylvie Maguin Sylvie Maguin Sylvie Maguin Taftazani, Muhammad Rifqi Tri Murini Tri Murini Tri Wahono Tri Wahyuni Sukesi Tri Wibawa Tri Wibawa Tri Wibawa Tri Wibawa Tridjoko Hadianto Tridjoko Hadianto, Tridjoko Trisno Agung Wibowo Triwibowo Ambar Garjito Triwibowo Ambar Garjito Unida, Tazkiah Wardana, Ali Wulandari, Indah Sari Yahiddin Selian Yogik Onky Silvana Wijaya