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All Journal International Journal of Public Health Science (IJPHS) Tropical Medicine Journal Jurnal Kedokteran Brawijaya Vektora : Jurnal Vektor dan Reservoir Penyakit Jurnal Vektor Penyakit Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal Kesehatan Masyarakat Majalah Geografi Indonesia Jurnal Kesehatan Lingkungan Medula Indonesian Journal of Biotechnology Majalah Obat Tradisional Jurnal Manajemen Kesehatan Indonesia The Indonesian Biomedical Journal Jurnal Kesehatan Vokasional Jurnal Kedokteran dan Kesehatan Journal of Public Health for Tropical and Coastal Region Unnes Journal of Public Health YARSI Medical Journal Jurnal Kesehatan Terpadu (Integrated Health Journal) Journal of Community Empowerment for Health ASEAN Journal of Community Engagement BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) Jurnal Kesehatan Tambusai Berita Kedokteran Masyarakat Jurnal Manajemen Pelayanan Kesehatan (The Indonesian Journal of Health Service Management) Indonesian Journal of Biomedicine and Clinical Sciences Jurnal Pengabdian, Riset, Kreativitas, Inovasi, dan Teknologi Tepat Guna (Parikesit) ASPIRATOR - Journal of Vector-Borne Diseases Studies Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)

Tri Baskoro Tunggul Satoto

Departemen Parasitologi, Fakultas Kedokteran Kesehatan Masyarakat Dan Keperawatan, Universitas Gadjah Mada, Yogyakarta, Indonesia

Author-ID : 374386

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Penentuan Wilayah Reseptif Malaria di Perbukitan Menoreh dengan Menggunakan Basis Data Nasional Kebijakan Satu Peta Barandi Sapta Widartono; Suharyadi Suharyadi; Tri Baskoro Tunggul Satoto; Mujiyanto Mujiyanto
Jurnal Kesehatan Vokasional Vol 7, No 3 (2022): Agustus
Publisher : Sekolah Vokasi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jkesvo.75453

Latar Belakang: Program eliminasi di Indonesia ditargetkan pada tahun 2030, tetapi hingga saat ini masih mengalami beberapa kendala, seperti terjadinya penyebaran kasus malaria secara impor melalui migrasi. Pendekatan yang umum digunakan saat ini adalah pendekatan wilayah administrasi, sedangkan pendekatan habitat nyamuk belum banyak dilakukan.Tujuan: Membuat pemetaan daerah reseptif malaria berbasis faktor lingkungan habitat nyamuk yang berkaitan dengan wilayah endemis di Perbukitan Menoreh yang meliputi Kabupaten Purworejo, Kabupaten Magelang, dan Kabupaten Kulon Progo.Metode: Penentuan daerah reseptif malaria dilakukan dengan pendekatan habitat nyamuk menggunakan faktor lingkungan fisik dari basis data spasial nasional Kebijakan Satu Peta (KSP).Hasil: Peta daerah reseptif dapat dihasilkan dari ekstraksi KSP. Selain itu, penanganan malaria pada model spasial ini dapat menunjukkan persentase dan luas area yang benar-benar memiliki ancaman terhadap kejadian malaria.Kesimpulan: Wilayah reseptif dengan model ini dapat memberikan gambaran jangka panjang ancaman malaria, menghasilkan sebaran wilayah reseptif dengan lebih baik dan mudah diberlakukan secara nasional.

PELAPORAN SURVEILANS DEMAM BERDARAH BERBASIS SMS DI KOTA YOGYAKARTA Anwar Dwi Cahyono; Tri Baskoro Tunggul Satoto; Lutfan Lazuardi
Journal of Health Service Management Vol 19 No 2 (2016)
Publisher : Departemen Kebijakan dan Manajemen Kesehatan, Fakultas Kedokteran, Kesehatan Masyarakat, dan Keperawatan, Universitas Gadjah Mada, Yogyakarta Jl. Farmako Sekip Utara Yogyakarta 55281 Telp 0274-547490

Background: The need of accurate and accelerate informationlead to decision making in order to dengue treatment now adays is high in Yogyakarta City Government. This was related tothe fact that there is a delay more than 24 hours from patient isdiagnosed with dengue until the case was reported fromhospital. Objective: to describe dengue surveillance report using SMSgateway. Methods: This research is using qualitative approach.Respondent from this research are Head of Disease ControlDepartment, Head of Health Promotion and Health InformationSystem and the others are hospital staffs who is responsible fordengue reporting. Result: SMS based reporting does not show the real amountnumber of dengue cases for a year, during the implementationthere has been 187 cases from the SMS. This was far beyondthe real number of cases which is 411 cases. Conclusion: This SMS based for dengue surveillance reportingcannot be the information that can lead to decision making.

Pengaruh Enzim Taq Polimerase dan Suhu Annealing terhadap Amplifikasi Gen Tropomyosin Sarcoptes scabiei Perdana, Taufik Mulya; Dwiputro, Alfin Harjuno; Wijaya, Yogik Onky Silvana; Satoto, Tri Baskoro Tunggul
Jurnal Kedokteran dan Kesehatan Vol 20, No 2 (2024): JURNAL KEDOKTERAN DAN KESEHATAN
Publisher : Faculty of Public Health, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jkk.20.2.138-145

Skabies adalah penyakit kulit yang menjadi masalah kesehatan masyarakat, terutama di negara berkembang. Hingga saat ini, belum ada alat bantu diagnostik yang dapat digunakan untuk menunjang diagnosis skabies. Salah satu kandidat alat diagnostik yang gencar dikembangkan adalah uji imonologis yang mendeteksi antibodi anti-protein struktural dari Sarcoptes scabiei seperti tropomyosin. Selama ini, pembuatan tropomyosin rekombinan menggunakan sumber berupa copy DNA (cDNA) yang cenderung mahal. Untuk menekan beban produksi, digunakanlah genomic DNA (gDNA) sebagai sumber materi genetik. Akan tetapi, pembuatan tropomyosin rekombinan dari gDNA belum pernah dikerjakan. Oleh karena itu, dilakukanlah studi optimisasi polymerase chain reaction (PCR) ini sebagai langkah awal pengembangan alat bantu diagnostik tersebut. Penelitian ini bertujuan untuk mengetahui kondisi amplifikasi gen tropomyosin yang optimal dengan mempelajari efek penggunaan MyTaqTM HS Red Mix (MeridianBioscience) and GoTaq® Green Master Mix. (Promega) serta suhu annealing yang berbeda pada amplifikasi gen tropomiosin S. scabiei. Hasil PCR dengan MyTaqTM HS Red Mix pada suhu annealing 57.1°C, 60.9°C, 63.4°C, dan 65°C menghasilkan pita yang terlihat jelas. PCR dengan menggunakan GoTaq® Green Master Mix tidak menghasilkan amplifikasi DNA. Oleh karena itu, amplifikasi gen tropomiosin S. scabiei paling baik dilakukan dengan menggunakan MyTaqTM HS Red Mix, dengan suhu annealing 65°C.

Innovation in vector control of dengue hemorrhagic fever using portable devices mechanical electric methods Alvira Pascawati, Nur; Baskoro Tunggul Satoto, Tri; Mustiadi, Ikhwan; Lusiana Utari, Evrita
International Journal of Public Health Science (IJPHS) Vol 13, No 3: September 2024
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v13i3.24218

This innovation was created to control the dengue hemorrhagic fever (DHF) vector population in the larval cycle because several previous control methods were considered less sensitive, effective, and efficient. To test the effectiveness of portable electrical-mechanical methods in controlling Aedes aegypti larvae. The research design was posttest only which was tested on 80-liter and 90-liter volume containers in light and dark colors. Statistical analysis used the independent samples t-test, and Pearson correlation (α=5%). Laboratory test results show that the average time needed to suck all the larvae in a light-colored container with a volume of 80 liters is 38 seconds (28-57 seconds) and a volume of 90 liters is 99.6 seconds (80-119 seconds). In a dark-colored container, the 80-liter volume is 50.8 seconds (3-89 seconds) and the 90-liter volume is 106.8 seconds (88-122 seconds). This tool sucks larvae faster in containers with a volume of 80 liters compared to a volume of 90 liters. The greater the volume of water and the higher the water surface, the longer it takes to suck the larvae. This tool is effective and efficient in speeding up the process of monitoring, collecting, and controlling larvae in large containers.

Determinant factor affected the presence of Aedes sp. in a customary village Pascawati, Nur Alvira; Satoto, Tri Baskoro Tunggul
International Journal of Public Health Science (IJPHS) Vol 12, No 3: September 2023
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijphs.v12i3.22996

Vector surveillance and control are the only reliable means for the prevention and control of dengue fever and dengue hemorrhagic fever, such as in Customary Village environments. Indonesia is an archipelagic country that has many Customary Villages, so the variables that affect the breeding of dengue vectors are numerous and complex. This study identifies potential vector breeding places and environmental factors for the presence of Aedes sp. in Customary Villages. A study with a cross-sectional design was conducted in the Segenter Customary Villages, West Nusa Tenggara, Indonesia. Simple and multiple logistic regression analysis to identify risk factors associated with the presence of Aedes larvae. The results showed that the entomological index was in the high category and at risk as a potential breeding place for the Aedes sp. The determinant factor that affects the presence of dengue vectors is the traditional type of house (p-value=0.005; RP=2.138). This factor is strengthened by the 70-90% humidity (p-value= 0.009; RP=1.764), 25-30 oC house temperatures (p-value=0.011; RP=1.650), and mixed type house (p-value=0.067; RP=1.413). These findings contribute to the development of an early warning system regarding the potential spread of Aedes vector-borne diseases, increase awareness, promote and prevention of dengue fever (DF), and its vectors to healthy tourism in Indonesia.

Peningkatan Pengetahuan dan Persepsi terhadap Penyakit Demam Berdarah Dengue pada Santri di Pondok Pesantren dengan Metode Ceramah Edukasi Tri Baskoro Tunggul Satoto; Dwiputro, Alfin Harjuno
Jurnal Pengabdian, Riset, Kreativitas, Inovasi, dan Teknologi Tepat Guna Vol 1 No 2 (2023): November
Publisher : Direktorat Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/parikesit.v1i2.9662

Dengue hemorrhagic fever (DHF) is a vector-borne disease which posses a great threat for public health sector in many countries, including Indonesia. It is estimated more than 390 million cases are reported annually worldwide. Indonesia is one of the countries with the highest number of dengue cases in the world, with 95,000 cases recorded in 2018. Vector control is the main strategy for preventing the transmission of Dengue infection. Vector control practices are influenced by the level of public knowledge and perception. This study aimed to determine and improve the level of knowledge and perceptions of Islamic boarding school students regarding DHF transmission. This was a quasi-experimental research with one group pretest & posttest. The interventions carried out were in the form of health promotion with educational lectures on the prevention and transmission of DHF. The level of knowledge and perception was measured before and after the intervention using a questionnaire. The number of respondents in this study were 166 respondents, with most of them are female (50.6%). There was an increase in the average posttest scores compared to the pretest with respective averages of 63.84 ± 12.02 and 75.74 ± 11.78 (p = 0.0001). The existence of health promotion in the santri group could increase knowledge and perception of DHF as a vector-borne disease.

Indeks Entomologi dan Faktor Jenis Rumah yang Berkaitan dengan Keberadaan Telur Aedes spp. di Kelurahan Sungai Pinang, Provinsi Jambi: Entomological Index and House Type Factors Related to the Existence of Aedes spp. Eggs in Sungai Pinang Urban Village, Jambi Province Unida, Tazkiah; Satoto, Tri Baskoro Tunggul; Garjito, Triwibowo Ambar; Martono, Edhi
Aspirator Vol 16 No 1 (2025): Jurnal Aspirator Volume 16 Nomor 1 2025
Publisher : Perkumpulan Entomologi Kesehatan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58623/aspirator.v16i1.117

Kelurahan Sungai Pinang memiliki kepadatan penduduk tinggi dan endemis dengue. Keberadaan Aedes spp. sebagai vektor virus dengue diperparah dengan kondisi lingkungan yang kurang terkontrol, seperti banjir yang memperbanyak wadah tergenang sebagai tempat perindukan nyamuk. Variasi tipe rumah juga mempengaruhi populasi Aedes spp., sehingga penelitian mengenai indeks entomologi dan jenis rumah yang mempengaruhi keberadaan telur Aedes spp. perlu dilakukan. Penelitian ini bertujuan untuk mengamati indeks entomologi Aedes spp. di Kelurahan Sungai Pinang (1°59'13.3" S dan 102°02'55.2" E) berupa house index (HI), container index (CI), breteau index (BI), ovitrap index (OI), dan angka bebas jentik (ABJ) di wilayah RT 07 dan RT 18 beserta kaitannya dengan jenis rumah sampling di lokasi penelitian. Penelitian ini menggunakan metode observasional dengan desain cross-sectional. Teknik sampling secara purposive dengan memilih RT dengan kasus dengue tahun 2023 – awal tahun 2024. Data indeks entomologi dianalisis secara univariat, sedangkan analisis korelasi Spearman dilakukan secara bivariat. Hasil penelitian menunjukkan HI sebesar 22,35%, CI 15,22%, dan BI 63,92%. Nilai HI dan CI termasuk dalam kategori densitas sedang, sedangkan BI termasuk kategori tinggi. Angka bebas jentik di RT 07 dan RT 18 adalah 77,65%, masih di bawah target nasional 95%. Ovitrap index mencapai 89,41%, menunjukkan tingkat resiko tinggi. Rumah yang ditemukan telur nyamuk > 10 buah adalah rumah semi-permanen dengan celah di dinding kayu dan atap tanpa plafon. Kelurahan Sungai Pinang masuk dalam kategori berisiko tinggi terjadi penularan dengue.

Genotyping F1534C mutation on dried Aedes aegypti preparation through direct PCR method: a proof of concept Perdana, Taufik Mulya; Wijaya, Yogik Onky Silvana; Dwiputro, Alfin Harjuno; Najla, Aesha; Taftazani, Muhammad Rifqi; Insani, Dini Aura; Pangesti, Rachma Widya; Satoto, Tri Baskoro Tunggul
Indonesian Journal of Biomedicine and Clinical Sciences Vol 57 No 3 (2025)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v57i3.17975

Aedes aegypti (Ae. aegypti) is the primary vector of dengue hemorrhagic fever (DHF). Various control strategies have been utilized to control its population, including the use of pyrethroid-based insecticides. However, the presence of mutations, such as the F1534C mutation, that confers resistance to pyrethroids has been increasingly reported. The increase of resistance-conferring mutation in Ae. aegypti population could potentially hinder DHF control measures. As such, monitoring the genotype of Ae. aegypti population is crucial. Mosquito rearing, DNA extraction, and PCR examination are usually employed to monitor the circulation of F1534C mutations. To simplify this process, we proposed a direct PCR workflow utilizing dried mosquito samples preserved on an in-house filter paper. To demonstrate the utility of our proposed workflow, we performed direct allele-specific PCR (AS-PCR) on 46 dried adult Ae. aegypti. As a comparator, conventional PCR was performed on 8 DNA extract from Ae. aegypti. Our results showed that direct AS-PCR successfully identified both wild-type (F allele) and mutant (C allele) genotypes from dried mosquitos with a success rate of 93.48%. These findings provide preliminary evidence supporting the use of cellulose-based in-house filter paper for genotyping insecticide-resistant mosquitoes. However, field testing must be performed before its implementation in real-world epidemiological and surveillance applications.

Entomological Index and Home Environment Contribution to Dengue Hemorrhagic Fever in Mataram City, Indonesia Satoto, Tri Baskoro Tunggul; Pascawati, Nur Alvira; Wibawa, Tri; Frutos, Roger; Maguin, Sylvie; Mulyawan, I Kadek; Wardana, Ali
Kesmas Vol. 15, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Indonesia is a member of Southeast Asia Regional Office (SEARO) ranked the first in dengue hemorrhagic fever (DHF) problem based on incidence rate (IR) and case fatality rate (CFR). Several provinces in Indonesia experience an outbreak, one of which is the Mataram City in West Nusa Tenggara Province. Mataram City is an endemic area of DHF because the DHF cases are always found in three consecutive years with the number of cases that fluctuate and tend to increase. This study aimed to obtain factors that could be used to improve early warning systems in controlling DHF. This study used a case control design with a ratio of 1:1 to 180 house holds. The results showed that home environmental factors, such as no ceiling, indoor and outdoor temperature that had the potential for breeding places for mosquitoes, no wire net in ventilation, low lighting and high humidity, related to DHF transmission. Vector distibution with entomology index showed that the existence of larvae, eggs and mosquitoes played a role in dengue transmission. The dominant factors affecting the transmission of dengue in Mataram City are the condition of the ceiling and the existence of mosquito eggs in the house.

Controlling Factors that Potentially against Transmission of Dengue Hemorrhagic Fever at State Elementary Schools in Yogyakarta Satoto, Tri Baskoro Tunggul; Alvira, Nur; Wibawa, Tri; Diptyanusa, Ajib
Kesmas Vol. 11, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Sekolah dasar merupakan tempat yang dapat mengakibatkan peningkatan penyakit dengue pada anak karena faktor lingkungan, adanya potensi penularan, belum adanya system manajemen lingkungan yang baik dan beberapa upaya pengendalian yang tidak lagi efektif. Penelitian ini bertujuan untuk mengetahui faktor yang berpotensi terhadap penularan demam berdarah dengue bagi anak, sehingga sistem kewaspadaan dini dapat ditegakkan. Jenis penelitian ini adalah analitik dengan rancangan potong lintang. Penelitian dilaksanakan pada 91 sekolah dasar negeri di Kota Yogyakarta. Variabel yang diobservasi adalah serotype virus DEN, resistensi insektisida, kepadatan vektor,dan kondisi fisik sekolah. Analisis data menggunakan analisis deskriptif dan korelasi-regresi (a = 0.05). Hasil menunjukkan bahwa terdapat delapan sekolah dengan virus dengue serotip 2 and 3, terjadi resistensi insektisida organofosfat tingkat ringan dan sedang, lingkungan sekolah telah rentan terhadap penularan demam berdarah berdasarkan container index, house index, breteau index dan ovitrap index, suhu dan kelembaban di dalam dan luar ruangan berpotensi terhadap tingginya kepadatan telur, ventilasi tidak terpasang kawat kasa, dan jarak antara bangunan sangat dekat dapat menyebabkan penularan menjadi sangat cepat. Elementary school is a place that can result in increase of dengue disease among children because of environmental factors, potential transmission, the absence of good environmental management system and some control efforts which are no longer effective. This study aimed to determine factors that potentially against transmission of dengue hemorrhagic fever in state elementary school, so the early warning system can be enforced. Type of study was analytic with cross-sectional design. The study was conducted in 91 state elementary schools in Yogyakarta City in 2014. Variables in the observation are virus serotype DEN, insecticide resistance, the density of vector and physical condition of schools. Data analysis used descriptive and correlation-regression (a = 5%). Results showed that there were eight schools with dengue virus serotype 2 and 3 mosquitoes declared to have mild and moderate resistance to organophosphate, the school environment was susceptible to transmission of dengue hemorrhagic fever based on the container index, house index, breteau index and ovitrap index, temperature and humidity inside and outside were potential to the high density of eggs, wire netting was not installed on ventilation and the very close distance between the buildings could lead to transmission.

Co-Authors Achmad Fudholi Ajib Diptyanusa Ajib Diptyanusa Ajib Diptyanusa Alamri, Annisa Rizqa Ali Wardana Ali Wardana Alvin Harjono Dwiputro Alvira Pascawati, Nur Angle Maria Hesti Sorisi Anies Mulyani Anis Fuad Anwar Dwi Cahyono Ardhi, Khairil Ariyanto Nugroho Barandi Sapta Widartono Barandi Sapta Widartono Budi Mulyaningsih Budi Mulyaningsih Budi Mulyaningsih Budi Mulyaningsih Damar Tri Boewono Diptyanusa, Ajib Diptyanusa, Ajib Dwi Aris Agung Nugrahaningsih Dwi Sarwani Sri Rejeki Dwiputro, Alfin Harjuno Dyah Mahendrasari Sukendra E. Elsa Herdiana Murhandarwati Edhi Martono Elisabeth Deta Lustiyati Ellen Petra Songjanan Ellen Petra Songjanan Ernaningsih Ernaningsih Ernaningsih Ernaningsih Frutos, Roger Fudholi, Achmad - Hakim, A. Ulil Fadli Hanani Melangwala Laumalay Hardaningsih, Ignatius Hari Kusnanto Hastuti Tongkodu I Kadek I Kadek Mulyawan I Made Swastika Ikhwan Mustiadi Insani, Dini Aura Joy Victor Imanuel Sambuaga Kurnia, Risman Kusumasari, Rizqiani Amalia Kuswati Kuswati Laksono, Rian Kurniawan Lilik Zuhriyah Lusiana Utari, Evrita Lutfan Lazuardi Lutfan Lazuardi Luthfan Lazuardi M Lutfan Lazuardi Mae Sri Hartati Wahyuningsih Maguin, Sylvie Mahardika Agus Wijayanti Mbiliyora, Agustina Mubarak Mubarak muhaimin saranani Mujiyanto Mujiyanto Mujiyanto Mujiyanto Mulyawan, I Kadek Najla, Aesha Pangesti, Rachma Widya Pascawati, Nur Alvira Perdana, Taufik Mulya Purwono Purwono R. Suharyadi Risdwiyanto, Rifa Nadhifa Ristiyanto Roger Frutos Roger Frutos Roger Frutos Ruben Wadu Wila Satrisno, Hary Setiawan, Yohanes Didik Siti Istianah Sitti Rahmah Umniyati Sitti Rahmah Umniyati Sitti Rahmah Umniyati Sitti Rahmah Umniyati Sitti Rahmah Umniyati Sitti Rahmah Umniyati Slamet Riyadi Suhermanto - Sulistyawati Sulistyawati Supriyati Sylvie Maguin Sylvie Maguin Sylvie Maguin Taftazani, Muhammad Rifqi Tri Murini Tri Murini Tri Wahono Tri Wahyuni Sukesi Tri Wibawa Tri Wibawa Tri Wibawa Tri Wibawa Tridjoko Hadianto Tridjoko Hadianto, Tridjoko Trisno Agung Wibowo Triwibowo Ambar Garjito Triwibowo Ambar Garjito Unida, Tazkiah Wardana, Ali Wulandari, Indah Sari Yahiddin Selian Yogik Onky Silvana Wijaya

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