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Journal : Planta Tropika

Secondary Metabolites Application of Two Pseudomonas fluorescens isolates and Two Trichoderma Harzianum Isolates in Combination Against Postharvest Anthracnose in Papaya Muarifah, Mizar; Soesanto, Loekas; Sastyawan, Murti Wisnu Ragil; Mugiastuti, Endang; Farid, Noor
PLANTA TROPIKA Vol 11, No 2 (2023)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v11i2.14594

Abstract

The occurrence of papaya anthracnose is a significant post-harvest ailment, necessitating the effective disease management. The aim was to determine the ability of secondary metabolites combination of Pseudomonas fluorescens and Trichoderma harzianum isolates against the disease. A completely randomized design was used for in vitro experiments and a randomized block design for in vivo experiments. The treatments consisted of P. fluorescens P60 and T. harzianum T10, P. fluorescens P60 and T. harzianum T213, P. fluorescens P32 and T. harzianum T10, P. fluorescens P32 and T. harzianum T213 secondary metabolites and fungicides (a.i. maneb). The observed variables included the pathosystem component and papaya character and organoleptic test. The in vitro test results showed that P. fluorescens P60 and T. harzianum T10 and P. fluorescens P60 and T. harzianum T213 secondary metabolites inhibited the pathogen growth by 48.1075 and 43.4625%, respectively. The secondary metabolites of P. fluorescens P60 and T. harzianum T10 in vivo test results could delay the germination time by 12.63% and reduce the invasion area by 44.29%. All secondary metabolites had no effect on sugar content, hardness and sensory test. The combined secondary metabolites of P. fluorescens and T. harzianum are safe and does not affect papaya fruit quality.
Application of Biocontrol Product Bio P60 and NASA Liquid Organic Fertilizer on The Development of Fusarium Wilt and Yield of Shallot in Planta Soesanto, Loekas; Yusup, Adi Maulana; Sastyawan, Murti Wisnu Ragil; Mugiastuti, Endang; Suharti, Woro Sri
PLANTA TROPIKA Vol. 13 No. 1 (2025)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v13i1.24558

Abstract

Shallots are indispensable in all aspects of human life, and shallot production is always facing Fusarium wilt disease. Chemical control of the disease has failed, and environmentally friendly control alternatives are needed. One of them is Bio P60 biocontrol product combined with liquid organic fertilizer. Aims of the research was to assess the effectiveness of NASA liquid organic fertilizer and Bio P60 in controlling Fusarium wilt and its impact on shallot growth and yield in planta. This research was conducted in the field using polybag, for five months. Completely randomized block design was used comprising of two components with 16 treatments and 3 replicates. The variables observed were incubation period, disease intensity, AUDPC, plant height, number of leaves, number of tubers, plant wet weight, tuber wet weight, plant dry weight, and tuber dry weight. The results showed that the application of Bio P60 five times was the most excellent treatment in postponing the incubation period by 61.71%, suppressing disease incidence by 66.67%, and reducing AUDPC by 69.84%, increase growth and yield components such as plant height by 30.75%, number of leaves by 40.7%, number of bulbs by 75.6%, bulb fresh weight by 104.53%, blub dry weight by 51.1%, crop fresh weight by 48.24%, and crop dry weight by 49.77% compared to the control. The fertilizer application has no significant effect on all variables. There was no interaction between Bio P60 and NASA on all variables.