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The Effect of Incubation Time on the Quality of Post-Thawed Ram Sexed Sperm Solihati, Nurcholidah; Rasad, Siti Darodjah; Setiawan, Rangga
Livestock and Animal Research Vol 22, No 2 (2024): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v22i2.90802

Abstract

Objective: The sperm sexing using an albumin column requires an incubation process at a certain temperature for sperm to move through the albumin layer. It is thought that the incubation time can influence the quality of the semen resulting from post-thawing sexing. The aim of this research was to determine the effect of incubation time on the quality of sexed sperm post-thawing and to find out the optimum incubation time that provide the best sperm quality.Methods: This research was carried out in a completely randomized design, using three incubation times (T1= 45 minutes, T2= 60 minutes, T3= 75 minutes) with six replications. The parameters consisted of motility, abnormality and acrosome integrity of sexed sperm in the upper and bottom fractions. Data were analyzed using analysis of variance (Anova) and continued with the Duncan test.Results: The results showed that the incubation time had a significant effect on motility, intact acrosome cap and recovery rate, but had no significant effect on abnormality of post thawed sexed sperm. For both the upper and bottom fraction, incubation times of 45 and 60 minutes produced the best motility and recovery rate compared to 75 minutes, but the best intact acrosome cup was obtained from an incubation time of 45 minutes.Conclusions: Based on the research results, it can be concluded that the length of incubation time influences the quality of post thawed ram sexed sperm, and 45 minutes is the optimal incubation time to provide the best quality.
Cases of cow reproductive disorders at The Northern Bandung Dairy Farmer Cooperative by using Geographic Information System (GIS) approach Triyatjaya, Yoga; Solihati, Nurcholidah; Septiyani, Septiyani
Livestock and Animal Research Vol 21, No 1 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i1.63993

Abstract

Objective: This study aimed to determine the percentage of reproductive disorders in dairy cows at The Northern Bandung Dairy Farmer Cooperative (KPSBU Lembang) based on body condition score (BCS) and season factor and determined the distribution map.Methods: The object of this study was secondary data on dairy cows from 2019 to 2021 registered on the cooperative database. The parameters of this study were type, case percentage, risk factor, and distribution of reproductive disorders. The data were analyzed descriptively based on BCS and season factors.Results: The percentage results were presented in tables and figures after being processed using Microsoft Excel, while the distribution maps were processed using the QGIS application. The percentages of reproductive disorders in KPSBU Lembang in 2019–2021 respectively as follows: Retained placenta were 11.5%, 14.9%, and 14.4%; Dystocia were 11.5%, 12.6%, and 14.2%. Ovarian function disorders (follicular cyst and corpus luteum cyst) were 10.2%, 8.8%, and 9.9%; Endometritis was 9.3%, 9.5%, and 10.4%. Based on the distribution maps of reproductive disorders, it was known that all villages in Lembang District in 2021 had cows with reproductive disorders.Conclusions: The reproductive disorders in dairy cows at KPSBU Lembang had retained placenta, dystocia, ovarian function disorder (follicular cyst and corpus luteum cyst), and endometritis. Dystocia and retained placenta are the highest cases in KPSBU Lembang during the dry and rainy seasons. Areas that had dairy cows with unideal BCS became the highest area of each type of reproductive disorder cases.Objective: This study aimed to determine the percentage of reproductive disorders in dairy cows at The Northern Bandung Dairy Farmer Cooperative (KPSBU Lembang) based on body condition score (BCS) and season factor and determined the distribution map.Methods: The object of this study was secondary data on dairy cows from 2019 to 2021 registered on the cooperative database. The parameters of this study were type, case percentage, risk factor, and distribution of reproductive disorders. The data were analyzed descriptively based on BCS and season factors.Results: The percentage results were presented in tables and figures after being processed using Microsoft Excel, while the distribution maps were processed using the QGIS application. The percentages of reproductive disorders in KPSBU Lembang in 2019–2021 respectively as follows: Retained placenta were 11.5%, 14.9%, and 14.4%; Dystocia were 11.5%, 12.6%, and 14.2%. Ovarian function disorders (follicular cyst and corpus luteum cyst) were 10.2%, 8.8%, and 9.9%; Endometritis was 9.3%, 9.5%, and 10.4%. Based on the distribution maps of reproductive disorders, it was known that all villages in Lembang District in 2021 had cows with reproductive disorders.Conclusions: The reproductive disorders in dairy cows at KPSBU Lembang had retained placenta, dystocia, ovarian function disorder (follicular cyst and corpus luteum cyst), and endometritis. Dystocia and retained placenta are the highest cases in KPSBU Lembang during the dry and rainy seasons. Areas that had dairy cows with unideal BCS became the highest area of each type of reproductive disorder cases.