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All Journal HAYATI Journal of Biosciences METAMORFOSA Journal of Biological Sciences Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS Biogenesis: Jurnal Ilmiah Biologi KUKILA Advances in Tropical Biodiversity and Environmental Sciences Jurnal Pendidikan dan Sosial Humaniora

SANG KETUT SUDIRGA

Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 388150

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Veterinary Other

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PEMBERIAN EKSTRAK BUAH JUWET (Syzygium cumini L.) TERHADAP JUMLAH DAN MORFOLOGI SPERMATOZOA TIKUS PUTIH (Rattus sp.) JANTAN YANG TERPAPAR ASAP ROKOK Fitria Dwijayanti; A.A.S.A. Sukmaningsih; , Ni Made Rai Suarni; Sang Ketut Sudirga; Ni Made Susun Parwanayoni
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

The research to determine the effect of fruit extracts juwet on the number and morphology of spermatozoa were superbly exposed to smoke has been conducted. This study uses rats aged 3 month, 200-210 gram, total 24 rats consisting of 4 treatment, is control, smoke of cigarette, juwet fruit extracts, and smoke of cigarette to juwet fruit extracts. Each treatment consisting 6 replications, each consisting of 1 rat. Before, acclimatization of rat treated 7 days. Way of giving juwet fruit extracts the gavage method 2 ml once daily for 48 days, while the CMC-Na 0,5% is given control. The exposure to cigarette smoke is given from an aerator pump , to given once daily for 48 days. Data were analyzed with ANOVA, if they were 5% significantly different would be followed by a DMRT. The result showed that the number of abnormal sperm morphology were significantly diferent (p<0,05). The averange number of abnormal spermatozoa K1 group was higher than K3 group. The number of spermatozoa was not significant different between treatments. The average number of spermatozoa K1 lower than K3. This is because the smoke of cigarette increased formation of ROS and the resulting stress oxidative and cause cell damage tissues and organs, especially to the reproductive system. Juwet fruits extract have been able to improve the count and morphologyof rats spermatozoa expose to smoke. This case because of antioxidant from juwet fruit can be protect cell from the free radical attack. Keywords: Rattus sp, smoke of cigarette, java plum fruit, spermatozoa, , ROS, antioxidant.

POTENTIAL OF Pseudomonas sp. AND Ochrobactrum sp. ISOLATED FROM VARIOUS SOIL SAMPLE AS DEGRADING BACTERIA OF HIGH DENSITY POLYETHYLENE (HDPE) AND LOW DENSITY POLYETHYLENE (LDPE) PLASTIC Muhammad Inas Riandi; Retno Kawuri; Sang Ketut Sudirga
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

plastics on a large scale has an effect on the increase to the amount of world waste, such as High Density Polyethylene (HDPE)and Low Density Polyethylene (LDPE) plastics. This study, tried to isolate Pseudomonas and Ochrobactrum bacteria from varioussoil in dumped soil area and mangrove forest in Bali to find out the degradation ability of the bacteria against HDPE and LDPEplastics. The methods used in this study were pour plate method, Biochemical and morphology identification by using Bergey’sManual of Determinative Bacteriology 9th Edition book as a reference, screening test and the determination of bacterial species byusing Vitek 2 Compact System. Evaluation of degradation result was done by measuring the weight loss of plastics residues. Thisstudy has isolated 27 bacteria that estimated from genus Pseudomonas and Ochrobactrum with characteristics as basil shape, Gramnegative, positive catalase and classified as non-fermenter bacteria. Isolate 2 bacteria which isolated from Suwung dumped soil hasidentified as Ochrobactrum anthropi with the ability of HDPE degradation by 20% and Isolate 24 which isolated from Karangasemdumped soil has identified as Pseudomonas aeruginosa with the ability of HDPE degradation by 18,75%.Keywords : Plastics waste, Biodegradation, High Density Polyethylene and Low Density Polyetene

UJI VIABILITAS BENIH ANGGREK HITAM (Coelogyne pandurata Lindl.) DENGAN MASA SIMPAN YANG BERBEDA VEMY CLAUDIA; IDA AYU ASTARINI; SANG KETUT SUDIRGA
SIMBIOSIS Vol I, No 2, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.125 KB)

The black orchid (Coelogyne pandurata Lindl.) is an epiphytic orchid spesies that iscurrently endangered. To protect and maintain the genetic resources it is necessary to find theproper way to store seeds in a long time. The purpose this study is to determine the viability ofthe black orchid seeds sowed in organic medium with different storage period. The result showedthat the seeds sown in fresh condition has the highest percentage of growth up to 60%, seedswith storage period of 2 weeks has growth percentage of 20%, while the storage period of 4weeks has growth percentage of 2.5%. The growth phase of sowed seeds in fresh condition andseeds storage in 2 weeks periode is phase 4 (second leaf formed embryo) and seeds storage in 2weeks periode is phase 2 (green embryo). This suggest that the storage condition of black orchidseeds is influence physiology and seed viability.Keywords: Coelogyne pandurata Lindl., seed viability, storage periode

PERBANDINGAN KANDUNGAN MINYAK ATSIRI TANAMAN SEREH WANGI (Cymbopogon nardus L. Rendle) YANG DITANAM DI LOKASI BERBEDA Margareta Dacosta; Sang Ketut Sudirga; I Ketut Muksin
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

It has been conducted a research that aims to determine the ratio of essential oil content of citronella stalks (Cymbopogon nardus Rendle L.) grown in the lowlands of Denpasar and the highlands of Bedugul. The experiment was conducted by using experimental designs. The parameters measured were the volume and the level of citronella oil produced. The sample preparation of powdered citronella stalks was as much as 200 grams, macerated with 96% ethanol and evaporated with Vacuum rotary evaporator to form condensed extract. The research used quantitative methods. The volume of hydro distillation was to obtain a crude extract of citronella stalks and the identification of active compounds was conducted by using Gas Chromatography / Mass Spectrometry (GC-MS). It can be concluded that the levels of citronella essential oil stalks from the highland of Bedugul was higher than the lowland areas of Denpasar, while the quality of essential oil of the lowland of Denpasar was better than that of the Bedugul highland. The content of secondary metabolites of the results of GC-MS analysis obtained from the essential oils contained in the analyzed crude extract of citronella stalks namely the compound of Selina-6-en-4-ol (2287322), the compound of n-hexadecanoic acid (1238019) and the compound of Driman-8,11-diol from the lowland of Denpasar while the Selina compound-6-en-4-ol (1856137) from the highland of Bedugul. Keywords: Citronella, essential oil content, altitude

PENGARUH DOSIS DAN LAMA PERLAKUAN EKSTRAK DAUN KALIANDRA MERAH (Calliandra calothyrsus Meissn.) TERHADAP STRUKTUR HISTOLOGI GINJAL MENCIT (Mus musculus L.) Nur Assiam; Iriani Setyawati; Sang Ketut Sudirga
SIMBIOSIS Vol 2 No 2 (2014)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (714.775 KB)

This research aims to determine the effect of red calliandra (Calliandra calothyrsusMeissn.) leaf extrac on the histological structure of the kidney of mice (Mus musculus L.).Treatment was administered orally with varying doses. This research used a CompletelyRandomized Design in factorial pattern of two factors, doses (0 or control, 2, 4, and 6 mg/kgBW) and length of treatment (7, 14, and 21 days), so there are 12 combination groups with 3replications of each. Organ was collected on days 8, 15, and 22 to observe histologicalstructure of the kidney. Renal histological observation of edema, Bowman’s spaceconstriction, and protein deposition, showed no correlation between both factors, but a veryreal correlation occurs in the damage of fatty degeneration, hemorrhage, and nucleuspyknotic. Histological observation of glomerular congestion and infiltration of inflammatorycells did not show any correlation between dose and duration of treatment.Keywords: red calliandra, histopathology of kidney, male mice

EFEKTIVITAS EKSTRAK BUNGA KENANGA (Cananga odorata) UNTUK MENGHAMBAT PERTUMBUHAN JAMUR Colletotrichum acutatum PENYEBAB PENYAKIT ANTRAKNOSA PADA TANAMAN CABAI BESAR (Capsicum annum) Silviana Febrionita Meiskia Agung; Sang Ketut Sudirga; Anak Agung Ketut Darmadi
SIMBIOSIS Vol 10 No 1 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Jenis Colletotrichum acutatum merupakan jamur patogen penyebab penyakit antraknosa pada tanaman budidaya, salah satunya tanaman cabai. Pengendalian penyakit antraknosa umumnya menggunakan pestisida sintetis. Penggunaan pestisida sintetis secara terus menerus dapat menimbulkan efek samping yang merugikan, sehingga diperlukan senyawa lain untuk mengendalikan jamur patogen tersebut salah satunya melalui penggunaan fungisida nabati. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak bunga kenanga dalam menghambat pertumbuhan Colletotrichum acutatum dan untuk mengetahui golongan senyawa aktif yang terkandung dalam ekstrak bunga kenanga. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan tujuh perlakuan yaitu P0: kontrol negatif, P1: kontrol positif, P2: konsentrasi ekstrak 1% (b/v), P3: konsentrasi ekstrak 2% (b/v), P4: konsentrasi ekstrak 3% (b/v), P5: konsentrasi ekstrak 4% (b/v), P6: konsentrasi ekstrak 5% (b/v) dengan masing-masing perlakuan diulang sebanyak 4 kali. Parameter yang diamati yaitu terbentuknya diameter zona hambat, diameter koloni, kerapatan spora, dan uji fitokimia ekstrak. Hasil penelitian uji sumur difusi menunjukan zona hambat terbesar terdapat pada konsentrasi 5% (P6) yaitu sebesar 10,05 mm dan zona hambat terkecil pada konsentrasi 1% (P2) sebesar 7,53 mm. Hasil uji koloni menunjukan diameter koloni terkecil pada hari ke-10 terdapat pada konsentrasi 3% (P4) sebesar 7,09 cm dan diameter koloni terbesar pada konsentrasi 1% (P2) sebesar 8,46 cm. Hasil uji kerapatan spora menunjukan jumlah kerapatan spora terbesar terdapat pada konsentrasi 1% (P3) yaitu 3,24×104 spora/mL dan jumlah spora terkecil terdapat pada konsentrasi 3% (P4) sebesar 2,36×104 spora/mL. Golongan senyawa aktif yang ditemukan dalam ekstrak bunga kenanga meliputi alkaloid, steroid, flavonoid, fenolik, saponin dan tanin.

PEMANFAATAN Trichoderma spp. SEBAGAI BIOKONTROL Sclerotium rolfsii Sacc. PADA TANAMAN KEDELAI (Glycine max L.) Candra Dewi Oktaviawati; Sang Ketut Sudirga; Junita Hardini
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Sclerotium rolfsii Sacc. on soybeans was one of the diseases in the cultivation that caused a decrease in soybeans (Glycine max L.) production. One of the efforts in increasing the growth and the resistance of the soybean was by using the biological agent named Trichoderma spp. This study aimed to control the intensity of the Sclerotium rolfsii that can cause disease in soybeans. this research was conducted from July 2021 to September 2021 at the Biochemical laboratory and at the Green House owned by the Biology Department FMIPA, Udayana University. The research used an completely randomized design (CRD) with five treatments; a) growing media without the treatment (control); b) growing media + 10 g Trichoderma spp., and without S. rolfsii; c) growing media without Trichoderma spp., + 5 g S. rolfsii; d) growing media + 15 g Trichoderma spp., and 5 g S. rolfsii; e) growing media + 20 g Trichoderma spp., and 5 g S. rolfsii. In this research, the treatment was repeated 5 times and 25 combinations of treatments were obtained. Analysis of Variance (ANOVA) was used in this study to analyze the data, it was found that there was a different result <0,05, then it was continued by using the analysis of Post Hoc Duncan Test. According to the research, it was found that there were two different results. Based on in vitro method, it can be concluded that Trichoderma spp. can inhibit S. rolfsii by about 55,56%. Meanwhile, based on in vivo method, it can be concluded that Trichoderma spp. can inhibit S. rolfsii about 40% which the optimal dose of Trichoderma spp. was about 20 g, the highest plant height was 64.4 cm, the highest average dry weight of shoots was 5.1 g, the highest average dry weight of roots was 0.5 g, and the highest average pod weight was 17.0 g.

EFFECTIVENESS OF CINNAMON (Cinnamomum burmanni Blume) LEAF EXTRACT IN INHIBITING THE GROWTH OF THE MUSHROOMS Colletotrichum magnum Rossman & Allen CAUSES OF ANTHRACHNOSE DISEASE IN PAPAYA (Carica papaya Linnaeus) Wafiatul Fitriyah; Sang Ketut Sudirga; Ni Made Gari
SIMBIOSIS Vol 11 No 1 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i01.p09

Anthracnose is a disease which is often found attacking plants. This disease can cause dieback or leaf tip damage of mature plants then followed by infection in the fruits, Consequently, this can result in the decrease of productivity. One of the causes of anthracnose is the pathogenic fungus Colletotrichum magnum. This fungus can infect several cultivated plants, one of which is papaya plants. This study aimed to determine the effectiveness of cinnamon leaf extract (Cinnamomum burmanni. Blume) in suppressing the growth of the fungus C. magnum and to analyze the class of compounds contained in cinnamon leaf extract. The method used in this study was a Completely Randomized Design (CRD) with 1 negative control, 1 positive control, and 5 treatments with concentrations of 1%, 2%, 3%, 4%, 5%, each treatment was repeated 4 times and then tested phytochemical screening. The results of the invitro study showed that cinnamon leaf extract was able to inhibit the growth of C. magnum as seen from the MIC test results, namely a concentration of 0.6% with an inhibition zone diameter of 8.37 mm. The results showed that as the concentration of cinnamon leaf extract increased, the diameter of the inhibition zone obtained was getting bigger. what you get is bigger. The concentration of 5% that has the largest inhibition zone among the concentration treatments, which is 19.77 mm. The results of phytochemical screening on cinnamon leaf extract showed that the cinnamon leaf extract contains positive groups of alkaloids, steroids, phenolic compounds, saponins, flavonoids, and tannins. Keyword: anthracnose, Cinnamon leaves, Inhibition, Colletotrichum magnum.

Co-Authors A.A.S. A. Sukmaningsih Affrina Fauziah Anak A.G.R Dalem Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Ariyanti Ni Kadek Aulia Nurbaiti Barnadus Nggahu Lindi Djawa Candra Dewi Oktaviawati Deglory Tunmuni Dessy Sukma Wirastuti Dessy Sukma Wirastuti Dewa Ngurah Suprapta Fitria Dwijayanti I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Junitha I KETUT MUKSIN I P. G. Ardhana I.G.N. Bayu Wirayudha IDA AYU ASTARINI Ida Bagus Gede Darmayasa Ida Bagus Made Suaskara IRIANI SETYAWATI Junita Hardini Kadek Mardika Kadek Wegi Kurnilia MADE SUSUN PARWANAYONI Margareta Dacosta Muhammad Inas Riandi Ngurah Intan Wiratmini Ni Desak Made Aprilia Dewi Ni LUH ARPIWI Ni Made Gari Ni Made Rai Suarni Ni Putu Adriani Astiti Ni Wayan Sudatri Nur Assiam Nyoman Darsini Retno Kawuri Shelly Burgin Silviana Febrionita Meiskia Agung Susun Susun Prawanayoni VEMY CLAUDIA Wafiatul Fitriyah Yan Ramona Yusni Khusnita Pangesti

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