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All Journal Indonesian Journal of Medicine Jurnal Pengabdian Pada Masyarakat METHABDI Majalah Ilmiah METHODA Jurnal Kedokteran Methodist
Hadyanto Lim
Departement Of Pharmacology, Faculty Of Medicine, Universitas Methodist Indonesia, Medan
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miR-21 Expression in Grade I, II, and III Breast Cancer -, Juwita; Ompusunggu, Henny E.S.; Nauphar, Donny; Pardede, Sumondang M.; Lim, Hadyanto; Siregar, Yahwardiah
Indonesian Journal of Medicine Vol 1, No 1 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.473 KB)

Abstract

Background: Breast cancer is the second largest cancer in the world.The gold standard for breast cancer diagnosis and prognosis is grade assessment (mitotic, nuclear pleiomorphism, tubular formation), based on breast tissue histopathology. However, this assessment has limitation in that it is subjective and semi quantitative. A genetic biomarker known as  microRNA (miRNA) may regulate human gene expression by targetting mRNA. One of the miRNA, i.e. miR-21, is able to suppress tumor suppressor mRNA, resulting in increased cellular proliferation andneoplastic transformation, and decreased apoptosis. This study aimed to investigate the expression of miR-21in breast cancer with histopathologic grade I, II, and III.Subjects and Method: This was a descriptive study with cross-sectional design involving paraffin block (FFPE) samples of ductal-type breast cancer tissue. Sixty four samples were obtained from the department of Anatomical Pathology, Haji Adam Malik Hospital, Medan. Patients history were obtained through medical records followed by sample processing (Total RNA Isolation, cDNA Synthesis, and Examination of miR-21 Expression using Real-Time qPCR). MiR-21 expression from every hystopathologic grade was obtained using Fold Change Calculation.Results: Breast cancer occurred more frequently in age group of 30-49 years old. Based on the histopathologic grade, most breast cancer was found in grade II. Analysis of miR-21 expression showed that miR-21 expression was higher in grade III compared to other grade.Conclusion: Breast cancer occured more frequently in the productive age group.Most breast cancer were found in grade II. Increased miR-21 expression in grade III may suggest that miR-21 is an oncomiR, which plays an important role in the development of malignant breast cancer.Keywords: breast cancer, histopathology grade, miRNA, miR-21.Correspondence: Juwita. Masters Program in Biomedical Sciences, Faculty of Medicine, North SumateraUniversity, Medan. Mobile: 08125262339.Indonesian Journal of Medicine (2016), 1(1): 26-33https://doi.org/10.26911/theijmed.2016.01.01.04
MicroRNA-155 Tissue Expression in Patients with Breast Cancer at Dr Haji Adam Malik Hospital, Medan Ompusunggu, Henny Erina Saurmauli; -, Juwita; Nauphar, Donny; Pardede, Sumondang M.; Lim, Hadyanto; Siregar, Yahwardiah
Indonesian Journal of Medicine Vol 1, No 1 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.615 KB)

Abstract

Background: MicroRNA (miRNA) is noncoding-RNA that comprises 18-22 nucleotides. miRNA binds its gene targets in the 3-untranslated region (3-UTR), causing direct degradation of mRNA and repressed translation of RNA. MicroRNA regulates various biological processes, including cell proliferation, apoptosis, cell growth, cell differentiation, and metabolism. Various changes have been identified miRNA expression in different types of cancers. This study aimed to describe the expression of miR-155 in patients with breast cancer tissue in Haji Adam Malik Hospital in Medan.Subjects and Method: This was a descriptive study. A total sample was 64 paraffin tissue blocks was taken from female patients with breast cancer at the surgical oncology unit, Haji Adam Malik Hospital in 2013-2014. RNA sample were isolated and checked using a spectrophotometer followed by cDNA synthesis. Then this sample was analyzed for miR-155 expression by using Real Time qPCR. Histopathological grade data were obtained from the medical record.Results: The miR-155 expression in the breast cancer patients is higher than that in the UniSp6 RNA Spike-in (internal controls). miR-155 expression was 29.64, 28.44, 29.22, in grade-I, grade-II, grade-III breast cancer, respectively. UniSp6 RNA Spike-in was 22.30, 22.89, 23.10, respectively in the control groups. Thus, increased of miR-155 expression was the highest in grade I breast cancer patients.Conclusion: Increased miR-155 expression can be used as a biomarker for early diagnosis and prognosis of breast cancer.Keywords: miRNA, miR-155, breast cancer.Correspondence: Henny Erina Saurmauli Ompusunggu. Department of Cellular and Molecular Biology, Faculty of Medicine, HKBP Nommensen University, Medan. Email: ompusunggu.henny@gmail.comIndonesian Journal of Medicine (2016), 1(1): 20-25https://doi.org/10.26911/theijmed.2016.01.01.03
PENYULUHAN PEMBERDAYAAN MASYARAKAT DALAM PENCEGAHAN STUNTING DESA AJIJULU, KABUPATEN KARO Siahaan, Jekson Martiar; Silitonga, Hendrika Adriana; Lim, Hadyanto; Pasaribu, Sumihar M. R.; Tarigan, Menang Bastanta; Jamnasi, Juli; Sinatra, Jadeny; Simanjuntak, Salomo Garda Utama; Sembiring, Budi Darmanta; Pakpahan, Edwin Anto; Hutagalung, Sanggam B.; Sinurat, Suryati; Br. T. Tambun, Gita Sradha; Hakim, Enggrek Pelita
Jurnal Pengabdian Pada Masyarakat METHABDI Vol 3 No 1 (2023): Jurnal Pengabdian Pada Masyarakat METHABDI
Publisher : Universitas Methodist Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46880/methabdi.Vol3No1.pp11-14

Abstract

Community Service (PKM) conducted by the Faculty of Medicine at the Indonesian Methodist University provides counseling on stunting prevention. PKM was held on April 14-16 2023 in Ajijulu Village, Tigapanah District, Karo Regency, North Sumatra. Lectures, discussions and motivations are used as orientation methods. Question and answer discussions were held with the aim of gaining insight, understanding and solutions to solve the problems and limitations faced by the local community. The results of the PKM suggestions and research are very effective, considering that there are still many people who are ignorant of stunting prevention. This counseling increases public awareness about stunting prevention. Residents of Ajijulu must be aware and know the importance of preventing stunting, starting from the time of pregnancy and the first 1000 days of life for toddlers.
Pelatihan Penanganan Cardiac Arrest (Henti Jantung) dengan Menggunakan Metode Bantuan Hidup Dasar (BHD) Bagi Pengemudi Ojek Online Se Kota Medan Siahaan, Dwi Lunarta D. S.; Sinatra, Jadeny; Nazma, Diani; Lubis, Andriamuri P.; Tanjung, Qodri F.; Sitepu, John Frans; Hamdi, Tasrif; Hamdani, Irfan; Chalil, M. Jalaluddin A.; Yunafri, Andri; Irina, Sinta; Zainumi, Cut M.; Fadinie, Wulan; Silaen, Ester L. R.; Simbolon, Boyke M.; Siahaan, Jekson M.; Lim, Hadyanto; Anto, Endy Juli; Tobing, Paul S. M. L.; Rimbun, Surjadi; Hutasoit, Eka Samuel P.; Tambunan, Ronald T. H.; Tarigan, Juliyanti; Stephanie S., Kezia; Natalie S., Karen
Jurnal Pengabdian Pada Masyarakat METHABDI Vol 4 No 2 (2024): Jurnal Pengabdian Pada Masyarakat METHABDI
Publisher : Universitas Methodist Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46880/methabdi.Vol4No2.pp188-193

Abstract

Cardiac Arrest is the leading cause of death in the world including Indonesia, which can occur anytime and anywhere. The causes vary, ranging from fatigue, and underlying health conditions, to traffic accidents. Basic Life Support (BLS) is the initial step that can be taken to restore respiratory and circulatory function in individuals experiencing respiratory or cardiac arrest. It is important for online motorcycle taxi drivers to possess these skills, as they often spend time on the road and interact with various people in different situations. Perhimpunan Dokter Spesialis Anesthesiology dan Terapi Intensif (PERDATIN) North Sumatra Branch concluded a series of Kursus Penyegar dan Penambah Ilmu Anestesia (KPPIA) by providing basic life support training to 521 public transport or online motorcycle taxi drivers in the city of Medan on September 29, 2024. Held at the Gedung Serba Guna of the North Sumatra Provincial Government, the participants were divided into 52 groups guided directly by anesthesiologist specialists as instructors. By providing this education and training, they can be better prepared to face emergency situations on the road, thereby enhancing safety and service for passengers
PENYULUHAN KONTROL HIPERTENSI DAN DIABETES SEJAK DINI KEPADA JEMAAT GEREJA HKBP GEDUNG JOHOR RESORT, KECAMATAN MEDAN JOHOR Anto, Endy Juli; Hutasoit, Eka Samuel P.; Siahaan, Jekson M.; Rimbun, Surjadi; Manullang, Frengky S.; Tiji, Widjaya Taufik; Tambunan, Ronald Tunggul Hotmarojahan; Lim, Hadyanto; Pasaribu, Sumihar Maurist Rantos; Tarigan, Menang Bastanta; Jamnasi, Juli; Sinatra, Jadeny; Silitonga, Hendrika Adriana
Jurnal Pengabdian Pada Masyarakat METHABDI Vol 3 No 2 (2023): Jurnal Pengabdian Pada Masyarakat METHABDI
Publisher : Universitas Methodist Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46880/methabdi.Vol3No2.pp128-132

Abstract

Community Service (PKM) in the form of health education conducted by lecturers at the Faculty of Medicine, Indonesian Methodist University provides outreach about the benefits of controlling hypertension and diabetes mellitus from an early age. PKM was held on August 5 2023 at the HKBP church, Medan Johor District, North Sumatra. Lectures, question and answer discussions and motivation are used as orientation methods. A question and answer discussion was held with the aim of gaining insight and understanding to avoid complications from hypertension and DM. The results of PKM's socialization and work are very effective considering that there are still many people who have not received information about the benefits of vaccination through the basic immunization program. This education makes people more aware of the importance of routine control of health services. The HKBP church community must be aware and know the importance of early control in avoiding complications.
Penyuluhan Program SPA (Sekolah Bebas Ispa) Kepada Siswa SD Galilea Hosana Kids School Lumban Raja, Alex Maruli Tua; Siahaan, Dwi Lunarta Doderina; Jamnasi, Juli; Mega, Juli Yosa; Jeffrey, Jeffrey; Lim, Hadyanto; Herlen, Aldo; Gultom, Helmida; Koswara, Caroline; br. Sembiring, Rehulina; Sihite, Fiorella Natalia Kinanti; Dohude, Priscilla Putri Derlian; Brar, Dylan Amrirtraj; Fransella, Kezia; Panggabean, Kristina Dameria
Jurnal Pengabdian Pada Masyarakat METHABDI Vol 4 No 1 (2024): Jurnal Pengabdian Pada Masyarakat METHABDI
Publisher : Universitas Methodist Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46880/methabdi.Vol4No1.pp23-26

Abstract

RTI (Respiratory Tract Infection) is an acute upper and lower respiratory tract inflammation caused by infectious agents, namely infections of microorganisms or bacteria, viruses, without or accompanied by lung parenchyma. RTI disease in Indonesia is on the 10th list of most common diseases in hospitals. PKM FK-UMI aims to bring the SPA (RTI-Free School/Sekolah Bebas ISPA) program, increase knowledge about RTI, and prevent and reduce the occurrence of RTI to Galilea Hosana Kids Elementary School. The method used is to deliver material about the definition, symptoms, how to get RTI, and how to prevent it. The results of the activity show that Galilea Hosana Kids Elementary School students still do not understand the meaning of RTI, the signs and symptoms of decreased body immunity, how to increase body immunity, and how to prevent the spread of RTI. Counseling for elementary school students at Galilea Hosana Kids Elementary School was very effective in educating students about RTI.
Epigenetik dan Penuaan (Aging): Peluang Intervensi Molekuler untuk Hidup Sehat Lebih Lama Siahaan, Dwi Lunarta Docterina Sutanti; Lim, Hadyanto
Majalah Ilmiah METHODA Vol. 15 No. 1 (2025): Majalah Ilmiah METHODA
Publisher : Universitas Methodist Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46880/methoda.Vol15No1.pp62-68

Abstract

Aging is a complex biological process characterized by a decline in cellular function and increased susceptibility to degenerative diseases. In recent decades, research has shown that epigenetic modifications—such as DNA methylation, histone modification, and regulation by non-coding RNAs—play a key role in the aging process. These changes influence gene expression without altering the DNA sequence and contribute to cellular damage, chronic inflammation, and tissue dysfunction. As scientific understanding advances, various epigenetic-based molecular interventions have emerged, including epigenetic reprogramming, senolytic therapies, and pharmacological and lifestyle approaches targeting epigenetic mechanisms. Although challenges remain in terms of safety, specificity, and clinical applicability, these approaches offer promising potential for extending healthspan and improving quality of life in later years.
Anti-inflammatory and Anti-infertility Effects of Ethanol Extract of Turmeric (Curcuma longa) in Male Wistar Rats with Type 2 Diabetes Mellitus Induced by Streptozotocin-Nicotinamide-High Fat Diet Vivian, Vivian; Lim, Hadyanto; Siahaan, Jekson Martiar; Anto, Endy Juli; Ilyas, Syaffrudin; Ardinata, Dedi
Indonesian Journal of Medicine Vol. 10 No. 4 (2025)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/theijmed.2025.10.4.891

Abstract

Background: Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder associated with chronic inflammation and male reproductive dysfunction. The ethanol extract of turmeric (Curcuma longa) has demonstrated potential as an alternative therapeutic agent due to its anti-inflammatory and reproductive restorative properties. This study aims to analyze the anti-inflammatory and anti-infertility effects of the ethanol extract of Curcuma longa in male Wistar rats with T2DM induced by a streptozotocin-nicotinamide-high fat diet (STZ-NA-HFD). Subjects and Method: Thirty male Wistar rats were randomized into six groups (n=5): normal control (K1), diabetic control (K2), positive control (T2DM + metformin 45 mg/kgBW, K3), and three treatment groups receiving Curcuma longa extract at doses of 50 mg/kgBW (K4), 100 mg/kgBW (K5), and 150 mg/kgBW (K6). Diabetes was induced with STZ (55 mg/kgBW) and nicotinamide (120 mg/kgBW). Blood glucose, IL-6, testosterone levels, sperm count, and sperm motility were evaluated after 21 days of treatment. Results: The turmeric extract significantly reduced blood glucose levels (p<0.001) and IL-6 concentrations from 574.06±68.99 to 214.06±78.71 ng/mL at a dose of 150 mg/kgBW (p<0.001). Normal sperm motility increased significantly at 150 mg/kgBW (20.0±2.55%) compared to the diabetic control (8.40±2.07%) (p=0.008). Conclusion: The ethanol extract of Curcuma longa demonstrated significant anti-inflammatory activity by reducing IL-6 levels and improving glycemic control. Its anti-infertility effect was reflected by enhanced sperm motility, particularly at the 150 mg/kgBW dose. These findings indicate that Curcuma longa possesses potential as an adjuvant therapy for the management of T2DM and its associated complications.
PENYULUHAN PEMBERDAYAAN MASYARAKAT DALAM PENCEGAHAN STUNTING DESA AJIJULU, KABUPATEN KARO Siahaan, Jekson Martiar; Silitonga, Hendrika Adriana; Lim, Hadyanto; Pasaribu, Sumihar M. R.; Tarigan, Menang Bastanta; Jamnasi, Juli; Sinatra, Jadeny; Simanjuntak, Salomo Garda Utama; Sembiring, Budi Darmanta; Pakpahan, Edwin Anto; Hutagalung, Sanggam B.; Sinurat, Suryati; Br. T. Tambun, Gita Sradha; Hakim, Enggrek Pelita
Jurnal Pengabdian Pada Masyarakat METHABDI Vol 3 No 1 (2023): Jurnal Pengabdian Pada Masyarakat METHABDI
Publisher : Universitas Methodist Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46880/methabdi.Vol3No1.pp11-14

Abstract

Community Service (PKM) conducted by the Faculty of Medicine at the Indonesian Methodist University provides counseling on stunting prevention. PKM was held on April 14-16 2023 in Ajijulu Village, Tigapanah District, Karo Regency, North Sumatra. Lectures, discussions and motivations are used as orientation methods. Question and answer discussions were held with the aim of gaining insight, understanding and solutions to solve the problems and limitations faced by the local community. The results of the PKM suggestions and research are very effective, considering that there are still many people who are ignorant of stunting prevention. This counseling increases public awareness about stunting prevention. Residents of Ajijulu must be aware and know the importance of preventing stunting, starting from the time of pregnancy and the first 1000 days of life for toddlers.
PENYULUHAN KONTROL HIPERTENSI DAN DIABETES SEJAK DINI KEPADA JEMAAT GEREJA HKBP GEDUNG JOHOR RESORT, KECAMATAN MEDAN JOHOR Anto, Endy Juli; Hutasoit, Eka Samuel P.; Siahaan, Jekson M.; Rimbun, Surjadi; Manullang, Frengky S.; Tiji, Widjaya Taufik; Tambunan, Ronald Tunggul Hotmarojahan; Lim, Hadyanto; Pasaribu, Sumihar Maurist Rantos; Tarigan, Menang Bastanta; Jamnasi, Juli; Sinatra, Jadeny; Silitonga, Hendrika Adriana
Jurnal Pengabdian Pada Masyarakat METHABDI Vol 3 No 2 (2023): Jurnal Pengabdian Pada Masyarakat METHABDI
Publisher : Universitas Methodist Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46880/methabdi.Vol3No2.pp128-132

Abstract

Community Service (PKM) in the form of health education conducted by lecturers at the Faculty of Medicine, Indonesian Methodist University provides outreach about the benefits of controlling hypertension and diabetes mellitus from an early age. PKM was held on August 5 2023 at the HKBP church, Medan Johor District, North Sumatra. Lectures, question and answer discussions and motivation are used as orientation methods. A question and answer discussion was held with the aim of gaining insight and understanding to avoid complications from hypertension and DM. The results of PKM's socialization and work are very effective considering that there are still many people who have not received information about the benefits of vaccination through the basic immunization program. This education makes people more aware of the importance of routine control of health services. The HKBP church community must be aware and know the importance of early control in avoiding complications.
Co-Authors Ardinata, Dedi - Blondina Marpaung br. Sembiring, Rehulina Br. T. Tambun, Gita Sradha Brar, Dylan Amrirtraj Chalil, M. Jalaluddin A. Diani Nazma Dohude, Priscilla Putri Derlian Donny Nauphar Endy Juli Anto Endy Juli Anto Fadinie, Wulan Fransella, Kezia Gultom, Helmida Hakim, Enggrek Pelita Hamdi, Tasrif Hasni, Dita Henny Erina Saurmauli Ompusunggu Herlen, Aldo Hutagalung, Sanggam B. Hutasoit, Eka Samuel P Hutasoit, Eka Samuel P. Ilyas, Syaffrudin Irfan Hamdani, Irfan Irina, Sinta Jaminsen Sinaga Jeffrey ., Jeffrey Juli Jamnasi Juwita -, Juwita Koswara, Caroline Lubis, Andriamuri P. Lumban Raja, Alex Maruli Tua Manullang, Frengky S. Mega, Juli Yosa Natalie S., Karen Ompusunggu, Henny E.S. Pakpahan, Edwin Anto Panggabean, Kristina Dameria Pardede, Sumondang M. Pasaribu, Sumihar M. R. Pasaribu, Sumihar Maurist Rantos Rimbun, Surjadi Sembiring, Budi Darmanta Siahaan, Dwi Lunarta D. S. Siahaan, Dwi Lunarta Docterina Sutanti Siahaan, Dwi Lunarta Doderina Siahaan, Jekson Martiar Sihite, Fiorella Natalia Kinanti Silaen, Ester L. R. Silitonga, Hendrika Adriana Simanjuntak, Salomo Garda Utama Simbolon, Boyke M. Sinatra, Jadeny Sinurat, Suryati Siregar, Kamal B. Sitepu, John Frans Stephanie S., Kezia Suryati Sinurat Tambunan, Ronald T. H. Tambunan, Ronald Tunggul Hotmarojahan Tanjung, Qodri F. Tarigan, Juliyanti Tarigan, Menang Bastanta Tiji, Widjaya Taufik Tobing, Paul S. M. L. Umar Zein Vivian Vivian Yahwardiah Siregar YUNAFRI, ANDRI Zainumi, Cut M.