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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Gizi dan Pangan Jurnal Teknologi Dan Industri Pangan Jurnal Teknologi Industri Pertanian Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Veteriner Perspektif : Review Penelitian Tanaman Industri agriTECH Indonesian Journal of Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Indonesian Journal of Chemistry ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education JURNAL SELULOSA Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Mutu Pangan : Indonesian Journal of Food Quality Perspektif, Review Penelitian Tanaman Industri Annales Bogorienses

Maggy Thenawidjaja Suhartono

Departemen Ilmu Dan Teknologi Pangan, Fakultas Teknologi Pertanian, IPB University, Kampus IPB Dramaga, Bogor 16680

Author-ID : 389473

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Imunomodulator Activity of Alginate Oligosaccharides from Alginate Sargassum crassifolium Subaryono Subaryono; Rosmawati Perangiangin; Maggy Thenawidjaja Suhartono; Fransiska Rungkat Zakaria
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20 No 1 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Alginate oligosaccharides (AOS) are oligosaccharides produced from depolimerization of the alginate polymer, and is reported to have various biological activities. The study aims is to determine the effect of AOSproduction conditions and their effects on products and its activities as an immunomodulatory compound. Production of alginate oligosaccharides (AOS) enzymatically carried out with the help of alginate lyase enzyme produced from the bacterium Bacillus megaterium S245. Variation of incubation time is 2, 4, 6 and 8 hours at concentrations of alginate lyase enzyme addition of 25, 50, 75 and 100U. Treatment of enzyme concentration and the duration of incubation in the production of AOS produces a degree of polymerization (DP) 2-7. In vitro activity test showed AOS is have ability to induce cell proliferation of human lymphocytes.This type of cell lymphocytes proliferation induced by AOS is a CD 8 cells or cytotoxic T cell and non cell CD4 / CD8. AOS production conditions with the addition of alginate lyase enzyme 50 U and incubation period 2 hours has produce AOS with the highest index of lymphocyte proliferation 117.6+3.6% or an increase of 43.24% compared to the native alginat polymer.

Isolasi dan Identifikasi Bakteri Asam Laktat Penghasil Inhibitor Enzim HMG-KoA Reduktase dari Bekasam Sebagai Agen Pereduksi Kolesterol Rinto Rinto; Ratih Dewanti; Sedarnawati Yasni; Maggy Thenawidjaja Suhartono
agriTECH Vol 35, No 3 (2015)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

The purpose of this research was to obtain statins producer bacteria as a HMG-CoA reductase (HMGR) enzyme inhibitor to reduced cholesterol biosynthesis. Stages of this research were the isolation of compactin and lovastatin resistant bacteria, statin production, analysis of culture extracts to inhibition of HMG-CoA reductase and identification of bacteria. The results showed that the 20 isolates of compactin and lovastatin resistant bacteria, there are 5 bacterial isolates produced statins. They were L3.3.4; C3.4.2; C3.3.5; C3.4.4 and L3.3.3; with the statins content were 9.491; 1.536; 0.065; 0.060; and 0.040 ppm. Selection of the 5 bacterial isolates resulted 2 bacteria which had inhibition ability to HMGR enzyme activity. They were Lactobacillus acidophilus and Lactobacillus delbruckii sp. delbruckii with inhibitory ability were 66.67% and 58.33%, respectively.ABSTRAKPenelitian ini bertujuan memperoleh bakteri penghasil statin sebagai inhibitor enzim HMG-KoA reduktase (HMGR), penghambat sintesis kolesterol. Tahapan penelitian yang dilakukan adalah isolasi bakteri yang resisten terhadap compactin dan lovastatin, produksi statin, uji penghambatan ekstrak dari kultur bakteri terhadap HMG-KoA reduktase dan identifikasi bakteri. Hasil penelitian menunjukan bahwa dari 20 isolat bakteri yang resisten terhadap compactin maupun lovastatin, terdapat 5 isolat bakteri yang potensial menghasilkan statin, yaitu isolat L3.3.4; C3.4.2; C3.3.5; C3.4.4 dan L3.3.3; dengan kandungan statin berturut-turut adalah 9.491; 1,536; 0,065, 0,060, dan 0,040 ppm. Seleksi terhadap 5 isolat menghasilkan 2 bakteri yang mempunyai kemampuan penghambatan terhadap aktivitas enzim ¸Â•ÝWtu Lactobacillus acidophilus dan Lactobacillus delbruckii sp. delbruckii dengan kemampuan penghambatan berturut-turut adalah 66,67% dan 58,33%.

Sequences Analysis of a Gene Encoding Extracellular Xylanase in Streptomyces costaricanus 45I-3 S. Sipriyadi; Aris Tri Wahyudi; Maggy Thenawidjaja Suhartono; Anja Meryandini
Indonesian Journal of Biotechnology Vol 20, No 1 (2015)
Publisher : Universitas Gadjah Mada

Streptomyces costaricanus 45I-3 is a bacterial strain belongs to actinomycetes group isolated from peat soil. Thebacterium is known to produce extracellular xylanase. The aims of this study were to analyze DNA sequence andsub-clone gene involved in the synthesis of extracellular xylanase. Complete DNA sequence predicted to encodexylanase genes was isolated from bacterial genome using Inverse Polymerase Chain Reaction (I-PCR). Total DNAsequence of 1664 bp in size obtained from I-PCR consisted of two open reading frames (ORF) in opposite direction.ORF1 was 1029 bp and ORF2 (partial sequence) was 309 bp. Analysis sequence using BlastX indicated that ORF1was homologous with xylanase bacterium enrichment culture clone Xyl8B8 (GenBank accession No. AFH35005.1),i.e. 95% in identity and 99% in similarity. In addition, ORF2 was homologous with glyoxalase bacterium enrichmentculture clone Xyl8B8 (GenBank accession No. AFH35007.1), i.e. 95% in identity and 98% in similarity. Analysis ofamino acid sequence revealed that ORF1 consisted of 2 domains, i.e. glyco-hydrolase 11 (GH11) and CarbohydrateBinding Type 2 (CBM2). Active site was found at 130th amino acid on GH11 domain. Visualization of 3-dimensionstructure showed that 1029 bp fragment is of 19 areas.

PHYLOGENETIC AND ANTIGENIC STRUCTURE OF AVIAN INFLUENZA VIRUS OF H5N1 SUBTYPE ISOLATED FROM WATERFOWLS R Susanti; Retno Damajanti Soejoedono; I Gusti Ngurah Kade Mahardika; I Wayan Teguh Wibawan; Maggy Thenawidjaja Suhartono
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (542.593 KB)

A study was carried (1) to analyze the phylogenetic relationship of fragment hemaglutinin (HA) geneof avian influenza viruses (AIV) subtype H5N1 isolated from apparently healthy backyard waterfowls inWest Java with representative of animal and human isolates from Indonesia and some countries in Asia;(2) to find out cross-reactivity of those viruses with a standard Indonesian strain. Nucleotide sequences ofHA gene of AIV H5N1 from backyard waterfowls along with other H5N1 isolates of Indonesian and Asianorigin were aligned using with ClustalW of MEGA 3.1 program. Estimation of genetic distance and theconstruction phylogenetic tree were conducted by Neighbor Joining method and calculation of distancematrix using Kimura 2-parameter. Antigenic analysis was conducted using hemagglutination inhibition(HI) test. Result of phylogenetic analysis indicated that all viruses from backyard waterfowls form threedistinct sublineages. One lineage was located in Indonesia cluster and two lineages in Asia cluster. In thephylogenetic analysis, it was concluded that multiple introductions of AIV H5N1 to Indonesia have occurred.Six AI H5N1 viruses from backyard waterfowls (IPB1-RS to IPB6-RS) appeared to be different ancestorsthose isolated previously in Indonesia. Cross-antigenic analysis showed that nine viruses isolates used inthis study were antigenically different to Legok 2003 chicken strain of AIV H5N1. The HI titer of anti-Legok 2003 antibody with all newly isolated viruses is up to 6 log lower then the HI titer using homologstrain.

KAKAO FERMENTASI : PELEPASAN PEPTIDA BIOAKTIF DAN MANFAATNYA BAGI KESEHATAN Fermented Cocoa: The Release of Bioactive Peptides and Their Health Benefits Winda Haliza; Endang Yuli Purwani; Dedi Fardiaz; Maggy Thenawidjaja Suhartono
Perspektif Vol 18, No 2 (2019): Desember 2019
Publisher : Puslitbang Perkebunan

ABSTRAKProses fermentasi diperlukan untuk mendapatkan biji kakao berkualitasi tinggi. Fermentasi biji kakao melibatkan beragam mikrobia dan enzim endogen yang mampu merombak komponen di dalamnya menjadi prekursor citarasa dan aroma bahkan komponen bioaktif. Protein termasuk salah satu komponen yang mengalami perombakan yang memicu pelepasan bioaktif peptida. Proses proteolitik selama fermentasi kakao menyediakan asam amino dan peptida yang melimpah dimana lebih dari 800 peptida dapat diidentifikasi secara jelas. Peptida tersebut memiliki manfaat kesehatan karena mampu berfungsi sebagai antioksidan, antihipertensi, antitumor dan sebagainya. Hal ini mengindikasikan bahwa biji kakao terfermentasi memiliki keunggulan sebagai sumber bioaktif peptida. Ketentuan fermentasi biji kakao di Indonesia secara jelas telah diatur oleh Peraturan Menteri Pertanian Republik Indonesia No.67/Permentan/Ot.140/5/2014 tentang Persyaratan Mutu dan Pemasaran Biji Kakao. Fermentasi spontan biji kakao bersifat unik dan berkaitan erat dengan keragamanan jenis mikroba dan enzim serta metabolit yang dihasilkannya. Pemahaman yang baik terhadap fermentasi spontan telah mendorong dikembang-kannya beragam teknologi fermentasi biji kakao yang sifatnya terkendali untuk menghasilkan produk dengan standar tertentu yang dikehendaki. Selanjutnya, proses fermentasi seharusnya menjadi strategi dalam meningkatkan daya saing biji kakao. ABSTRACTThe fermentation process is needed to get high-quality cocoa beans. Fermentation of cocoa beans involves a variety of microbes and endogenous enzymes that are able to remodel the components inside to become the precursors for flavor and aroma and even bioactive components. Protein is one component that has undergoes a change that triggers the release of bioactive peptides. Proteolytic processes during cocoa fermentation provide abundant amino acids and peptides from which more than 800 peptides can be clearly identified. The peptide has health benefits because it is able to function as an antioxidant, antihypertensive, antitumor and so on. This indicates that fermented cocoa beans have the advantage of being a source of bioactive peptides. The provisions on the fermentation of cocoa beans in Indonesia have clearly been regulated by Regulation of the Minister of Agriculture of the Republic of Indonesia No.67/Permentan/Ot.140/5/2014 concerning Quality and Marketing Requirements for Cocoa Beans. Spontaneous fermentation of cocoa beans is unique and is closely related to the variety of microbial types and the enzymes and metabolites that they produce. A good understanding of spontaneous fermentation has led to the development of a variety of cocoa bean fermentation technologies that are controlled to produce products with certain desired standards. Furthermore, the fermentation process should become a strategy to improve the competitiveness of cocoa beans.

Nasi Kaleng Sebagai Alternatif Pangan Darurat Elvira Syamsir; Sherly Valentina; Maggy Thenawidjaja Suhartono
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 1 No. 1 (2014): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Canned rice products were meeting the developed as Emergency Food Products (EFP) because of its convenience and stability as well as met eating habits of Indonesian people. The objective of this research was to produce canned-rice products as EFP that contribute the needs of daily energy intake (200 kcal), determine the effect of heat intensity during the thermal process (Fo) and rice variety to thermal characteristics and product quality. The rice formula consists of rice (36.87%), coconut milk (6.16%), block chicken broth (1.47%), salt (0.18%), and water (55.31%). The chicken formula consisted of cooked chicken meat (41.07%), coconut milk (32.86%), oil (8.21%), onion (3.09%), garlic (0.79%), nutmeg (0.55%), galingale (1.07%), coriander (0.03%), sugar (10.95% ) and salt (1.37%). Three types of rices with different amylose content, i.e.Cisadane (19.50%), IR 64 (23.88%) and IR 42 (28.24%) were used to make EFP. Thermal processing was carried out at different time–temperature schedules to achieve 15 and 20 minutes sterilized value (Fo). The product was packed in 307 x 113 silver enamel can and retorting at 121.1ºC (Tr) with CUT = 21 minutes. Amylose content and Fo value affected the color, texture and sensory properties of the products. EFP made of IR 64 and Fo value of 15 minutes was selected. The total energy value was 639.42 kcal per can (product’s weight was 200 g), which was contributed from fat (49.6%), protein (11.3%), and carbohydrate (39.1%).

Isolasi dan Identifikasi Bakteri Penghasil Alginat Lyase dari Rumput Laut Sargassum crassifolium Subaryono Subaryono; Rosmawaty Peranginangin; Maggy Thenawidjaja Suhartono; Fransiska Rungkat Zakaria
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): Juni 2015
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v10i1.239

Penelitian tentang isolasi dan identifikasi bakteri penghasil alginat lyase dari rumput laut coklat Sargassum crassifolium sudah dilakukan. Rumput laut didekomposisi selama 5 hari, dan bakteri dikultur dalam media luria bertani agar yang mengandung alginat 1 g/100 ml. Setelah diinkubasi selama 3 hari, bakteri yang tumbuh diisolasi dan diseleksi untuk mendapatkan isolat yang positif menghasilkan alginat lyase. Aktivitas alginat lyase ditandai dengan adanya zona bening di sekitar koloni setelah ditetesi dengan larutan 10% setil piridinium klorida. Hasil penelitian menghasilkan 4 isolat bakteri dengan indeks alginolitik tertinggi yaitu S245, S235, S155, dan S215. Identifikasi ke-empat isolat dilakukan secara morfologi, biokimia, dan genetik. Pelitian menunjukkan bahwa isolat S245 memiliki kemiripan dengan Bacillus megaterium, S235 memiliki kemiripan dengan Bacillus thuringiensis, S155 memiliki kemiripan dengan Bacillus cereus, dan S215 memiliki kemiripan dengan Bacillus pseudomycoides.

Physicochemical and Sensory Properties of Protein Isolate from Anchovy (Stolephorus insularis) Meda Canti; Katarina Aninda Karisma Palupi; Maggy Thenawidjaja Suhartono
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 17, No 1 (2022): May 2022
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.608

Anchovy is one of the primary fishery commodities in Indonesia; however its development as fishery products is currently suboptimal. Due to its high protein content, anchovy is potential to be developed as a source of value-added fish protein isolate. This study aims to produce anchovy protein isolate (API) and evaluate its physical, chemical, and sensory properties. The API was prepared from defatted anchovy flour. Isolation of anchovy protein was carried out using a pH-shifting method. The API was then analyzed for its physicochemical (bulk density, color, proximate) and sensory properties. The results showed that anchovy protein was more soluble at pH 11 and less at pH 5. Yield and protein recovery of API were 26.39 and 36.86% wb, respectively. The API had 92.20% protein, 3.64% moisture, 2.18% ash, 2.26% lipid, and 3.36% carbohydrate content on a dry basis. The results showed that the API exhibited good physical and sensory properties such as bulk density, color, the best score on sweetness, seaweed, bitterness, off-flavor, aroma, and rancid taste. There was no significant difference in sweet taste, off-flavor, aroma, and rancid taste between API and soy protein isolate (SPI) (p0.05). Overall, API demonstrated satisfactory nutritional properties and potential use as food ingredients.

Alginate Lyases: Sources, Mechanism of Activity and Potencial Application Subaryono Subaryono; Rosmawaty Peranginangin; Maggy Thenawidjaja Suhartono; Fransiska Rungkat Zakaria
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 3 (2013): December 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i3.39

Alginate lyases are group of enzymes which catalyze depolymerization of alginate into oligosaccharides. Alginate lyase have been widely used in many applications such as in production of bioactive oligosaccharides, control of polysaccharide rheological properties, and polysaccharide structure analysis. The products of alginate lyase, polysaccharide structure analysis, alginate oligosaccharides (AOS) have many biological activities including act as prebiotics, immune modulator, anticoagulation, antioxidant, anticancer, growth promoting activities, promote production of antibiotics and ethanol. In relation to the importance of alginate lyases, their potential aplications and prospect in development of new bioactive products, we present review of the enzymes, sources, mechanism of activity and potential applications. This paper also discussed some new biological engineering in alginate lyase production.

Alginate Lyase from Indonesian Bacillus megaterium S245 Shows Activities Toward Polymannuronate and Polyguluronate Subaryono Subaryono; Yuwanita Ardilasari; Rosmawaty Peranginangin; Fransisca Rungkat Zakaria; Maggy Thenawidjaja Suhartono
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 2 (2016): August 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i2.250

Screening of alginate lyase producing bacteria associated with seaweed Sargassum crassifolium was carried out, and isolate S245, identified as Bacillus megaterium S245 was found to produce high alginate lyase activity. This research was conducted to evaluate activity of the alginate lyase enzyme at various pHs, temperatures and substrates. Polymannuronate and polyguluronate were used to evaluate substrate specificities. Alginate lyase activity was assayed by analysis of reducing sugar released using the 3,5 dinitrosalicylic acid (DNS) method. The research showed that the activity of alginate lyase was optimum at pH of 7.0 and temperature of 45 0C. This enzyme was active for both polymannuronate and polyguluronate susbtrates. The Vmax and Km of this enzyme for polymannuronate and polyguluronate were 200 unit/ml/min and 79.8 mg/ml for polymannuronate substrate and 27.78 unit/ml/min and 13.17 mg/ml for polyguluronate substrate. This enzyme showed unique characteristic in working toward the two substrates.

Co-Authors , Widodo Abd. Rauf Patong Agnes Imelda Manurung Ahyar Ahmad ALBERTA RIKA PRATIWI Anja Meryandini Anja Meryandini Apon Zaenal Mustopa Apon Zaenal Mustopa Ardiansyah, Romadhony Aris Tri Wahyudi Bambang Prasetya DAHRUL SYAH Dedi Fardiaz Diana Lestari, Diana Diana Nur Afifah Efi Toding Tondok Ekowati Chasanah Elvira Syamsir Endang Yuli Purwani Eni Kusumaningtyas Eni Kusumaningtyas Enny Rimita Sembiring Evy Damayanthi Fitriyana, Intan Nur Fransisca Rungkat Zakaria Fransiska Rungkat Zakaria Fransiska Rungkat Zakaria Fransiska Rungkat Zakaria Haliza, Winda Hana Fitria Navratilova Hanifah Nuryani Lioe Harsi D. Kusumaningrum Hartati Chairunnisa Hasnah Natsir I Gusti Ngurah Kade Mahardika I wayan Teguh Wibawan Joshua Evan Katarina Aninda Karisma Palupi Kusmajadi Suradi Kusmajadi Suradi Laras Cempaka Laurentia Stephani Lilis Nuraida LILY MARIA GORETTI PANGGABEAN LINAWATI HARDJITO Meda Canti Meity Suradji Sinaga Muhammad Hanafi Palupi, Nurheni Sri Purwani, Endang Yuli Puspo Edi Giriwono R Susanti Raphaella Widiastuti Ratih Dewanti -Hariyadi Raymond R. Tjandrawinata Retno Damajanti Soejoedono Retno Damayanti Soejoedono Rinto . Rizki Maryam Astuti Rosmawati Perangiangin Rosmawaty Peranginangin Rosmawaty Peranginangin Rosmawaty Peranginangin Satya Nugroho SEDARNAWATI YASNI Setyani Budiari SHANTI RATNAKOMALA Sherly Valentina Sipriyadi Sipriyadi Sipriyadi Sri Budiarti Poerwanto Sri Sugiwati Subaryono Subaryono Subaryono Subaryono Subaryono Subaryono TATI NURHAYATI Wangsa Tirta Ismaya Wendry Setiyadi Putranto Winda Haliza Yanti Lim YOPI YOPI YULIN LESTARI Yuwanita Ardilasari

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