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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Gizi dan Pangan Jurnal Teknologi Dan Industri Pangan Jurnal Teknologi Industri Pertanian Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Veteriner Perspektif : Review Penelitian Tanaman Industri agriTECH Indonesian Journal of Biotechnology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Indonesian Journal of Chemistry ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education JURNAL SELULOSA Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Jurnal Mutu Pangan : Indonesian Journal of Food Quality Perspektif, Review Penelitian Tanaman Industri Annales Bogorienses

Maggy Thenawidjaja Suhartono

Departemen Ilmu Dan Teknologi Pangan, Fakultas Teknologi Pertanian, IPB University, Kampus IPB Dramaga, Bogor 16680

Author-ID : 389473

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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Angiotensin Converting Enzyme (ACE) Inhibitory Activity of Crude and Fractionated Snakehead Fish (Channa striata) Fillet Extract Setyani Budiari; Ekowati Chasanah; Maggy Thenawidjaja Suhartono; Nurheni Sri Palupi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 2 (2018): August 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i2.345

The existence of endogenous bioactive protein or peptide with angiotensin-converting enzyme (ACE) inhibitory activity in snakehead fish fillet is promising to be investigated. The purposes of this research were to extract ACE inhibitory endogenous protein or peptide from snakehead fish fillet and to fractionate the active compounds using ultrafiltration. The extraction employed two solvents, i.e. aquadest and 50% ethanol. Fractionation was conducted using ultrafiltration membranes of 10,000; 5,000 and 3,000 Molecular Weight Cut Off (MWCO) to separate the protein or peptide into the sizes of 10 kDa, 5-10 kDa, 3 -5 kDa and 3 kDa. The parameters observed were protein and peptide content, ACE inhibitory activity (in vitro) and also protein and peptide profiles. The result revealed that the snakehead fish fillet contained ACE inhibitory endogenous bioactive protein or peptide. The 50% ethanol was more effective in extracting peptide of 10 kDa than the aquadest. Yet, the aquadest was better in extracting higher molecular weight protein of 10 kDa than the 50% ethanol. The fraction of 3 kDa by aquadest had the highest ACE inhibitor activity per g protein (7.85% inhibition of ACE per g protein). Thus, the fraction of 3 kDa aquadest is the most promising option for further research and development of natural anti-hypertension compound. From the result, snakehead fish fillet was potential to be utilized as a functional food as well as functional ingredient to fight hypertension.

PRODUCTION AND CHARACTERIZATION OF CHITINASE ENZYMES FROM SULILI HOT SPRING IN SOUTH SULAWESI, Bacillus sp. HSA,3-1a Hasnah Natsir; Abd. Rauf Patong; Maggy Thenawidjaja Suhartono; Ahyar Ahmad
Indonesian Journal of Chemistry Vol 10, No 2 (2010)
Publisher : Universitas Gadjah Mada

Chitinase is an extracellular enzyme which is capable in hydrolyzing insoluble chitin to its oligomeric and monomeric components. The enzyme produced by thermophilic bacteria was screened and isolated from Sulili hot spring in Pinrang, South Sulawesi, Indonesia. The gram positive spore forming rod shape bacteria was identified as Bacillus sp. HSA,3-1a through morphological and physiological analysis. The production of chitinase enzyme was conducted at various concentration of colloidal chitin at a pH of 7.0 and a temperature of 55 °C. The bacteria optimally was produced the enzyme at a colloidal chitin concentration of 0.5% after 72 h of incubation. The optimum temperature, pH and substrate concentration of chitinase were 60 °C, 7.0 and 0.3%, respectively. The enzyme was stable at a pH of 7.0 and a temperature of 60 °C after 2 h of incubation. The chitinase activities was increased by addition of 1 mM Mg2+, Ca2+ and Mn2+ ions, whereas the activities were decreased by 1 mM Co2+, Fe2+ andZn2 ions. The molecular weight of the crude enzyme was determined using SDS-PAGE analysis. Five protein fractions were obtained from SDS-PAGE, with MWs of 79, 71, 48, 43 and 22 kDa.

Enterococcus faecium 1.15 Isolated from Bakasam Showed Milk Clotting Activity Wendry Setiyadi Putranto; Kusmajadi Suradi; Hartati Chairunnisa; Apon Zaenal Mustopa; Puspo Edi Giriwono; Harsi Dewantari Kusumaningrum; Maggy Thenawidjaja Suhartono
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The Lactic Acid Bacteria with Milk Clotting Activity (MCA) were isolated from Bakasam, an Indonesian traditional fermented meat. The isolate screening was carried out using modified method of Skim Milk Agar and Milk Clotting Activity Test, and the isolate was then identified using 16S rRNA. We found 4 isolates that showed MCA of 18-20 SU/ml. Identification using 16S rRNA indicated that the isolate ALG.1.15 was 99% (FR3-F primer) and 99% (FR3-R primer) identic with Enterococcus faecium. The isolate potentially produced renin-like protease to subtitute renin from veal.

ANALISIS KOMPARATIF MINERAL MIKRO DAN ANTI NUTRISI PADA BERAS ANTARA PADI REKAYASA GENETIK DAN TETUANYA Enny Rimita Sembiring; Puspo Edi Giriwono; Satya Nugroho; Maggy Thenawidjaja Suhartono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Comparative analysis is important aspect in food safety of transgenic crops to determine the effect of transgene on nutritional and anti-nutritional contents. This research was aimed to determine the concentration of Fe, Zn, phytic acid, and anti-trypsin activity, then assess the equivalence between transgenic rice and non-transgenic rice. Fe and Zn concentration was carried out using energy-dispersive x-ray fluorescence spectrometry. Anti-nutritional phytic acid and anti-trypsin activity were performed using visible light spectrophotometry. The data obtained were statistically tested using Independent sample t-test. These results indicated that the concentration of Fe, Zn, phytic acid, and anti-trypsin activity in 6 transgenic rice events were equivalent to non-transgenic rice. In conclusion, the transgene did not affect Fe, Zn, phytic acid, and anti-trypsin activities in brown rice of 6 transgenic rice events carrying cry1B::cry1Aa fusion genes. Analisis komparatif adalah salah satu aspek penting dalam keamanan pangan tanaman produk rekayasa genetik (PRG) untuk mengetahui pengaruh transgen terhadap kandungan nutrisi dan anti nutrisi. Pada penelitian ini dilakukan analisis komparatif konsentrasi mineral mikro Fe, Zn, asam fitat, dan aktivitas anti tripsin pada beras pecah kulit dari 6 galur padi PRG pembawa fusi gen cry1B::cry1Aa terhadap padi non-PRG Rojolele tetuanya. Analisis konsentrasi mineral mikro Fe dan Zn menggunakan metode energy-dispersive x-ray fluorescence spectrometry (ED-XRF) dengan 3 ulangan. Analisis konsentrasi anti nutrisi asam fitat dan aktivitas anti tripsin dilakukan menggunakan metode spektrofotometri sinar tampak dengan 8 ulangan. Data yang diperoleh diuji secara statistik menggunakan Independent sample t-test. Hasil yang diperoleh menunjukkan konsentrasi mineral mikro Fe, Zn, asam fitat, dan aktivitas anti tripsin pada beras dari 6 galur padi PRG pembawa fusi gen cry1B::cry1Aa setara dengan padi non-PRG Rojolele tetuanya. Kesimpulannya bahwa transgen tidak mempengaruhi kandungan mineral mikro Fe, Zn, asam fitat, dan aktivitas anti tripsin pada beras dari 6 galur padi PRG pembawa fusi gen cry1B::cry1Aa.

Produksi β-Glukosidase Aspergillus niger BIO 2173 dengan Fermentasi Padat Menggunakan Substrat Dedak Sri Sugiwati; Maggy Thenawidjaja Suhartono; Muhammad Hanafi; Hanifah Nuryani Lioe
JURNAL SELULOSA Vol 8, No 01 (2018): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Production of β-Glucosidase Aspergillus niger BIO 2173 on Solid State Fermentation Using Rice Bran as SubstrateAbstractβ-Glucosidase (EC 3.2.1.21) is a part of the cellulase enzyme complex which acts synergistically with exoglucanase and endoglucanase to hydrolyze cellulose into glucose. The purpose of this study was to obtain the maximum fermentation conditions for production of b-glucosidase Aspergillus niger BIO 2173 with solid state fermentation using rice bran as fermentation substrate. The factors that affect the production of b-glucosidase which consist of initial pH of the fermentation medium, incubation period, ratio of water content to fermentation substrate, incubation temperature and addition of the Mandel’s mineral salts solution were examined in the study. The results showed that maximum fermentation conditions for β-glucosidase production were at initial of fermentation pH of 2,0, incubation period of 7 days, ratio of water content to substrate of 1:1, and incubation temperature of 32oC. Addition of Mandel’s mineral salts solution to the fermentation substrate at maximum fermentation conditions increased the activity and specific activity of β-glucosidase crude extract up to 5,24 ± 0,57 U/mL and 2,46 ± 0,04 U/mg, respectively.Abstrakβ-Glukosidase (EC 3.2.1.21) merupakan bagian dari enzim multi kompleks selulase, yang bekerja secara sinergis dengan eksoglukanase dan endoglukanase menghidrolisis selulosa menjadi glukosa. Tujuan dari penelitian ini adalah mendapatkan kondisi fermentasi maksimum untuk produksi β-glukosidaseAspergillus niger BIO 2173 dengan fermentasi media padat menggunakan substrat dedak. Pengujian dilakukan terhadap faktor-faktor yang mempengaruhi produksi b-glukosidase, yaitu pH awal medium fermentasi, waktu inkubasi, perbandingan kandungan air terhadap substrat medium fermentasi, suhu inkubasi dan penambahan larutan garam mineral Mandels. Hasil penelitian menunjukkan bahwa kondisi fermentasi maksimum untuk produksi b-glukosidase adalah pada pH awal medium fermentasi 2,0; waktu inkubasi 7 hari, perbandingan kandungan air terhadap substrat medium fermentasi 1:1, dan suhu inkubasi 32oC. Penambahan larutan garam mineral Mandels ke dalam substrat fermentasi pada kondisi fermentasi maksimum menyebabkan peningkatan aktivitas dan aktivitas spesifk ekstrak kasar b-glukosidase masing-masing sebesar 5,24 ± 0,57 U/mL dan 2,46 ± 0,04 U/mg protein. Kata kunci: β-glukosidase, Aspergillus niger, dedak padi, fermentasi padat, ekstrak kasar

Karakterisasi Fisiko-Kimia Biji dan Kulit Ari Kacang Bogor Asal Jampang-Sukabumi Jawa Barat Rizki Maryam Astuti; Nurheni Sri Palupi; Maggy Thenawidjaja Suhartono; Hanifah Nuryani Lioe; Eni Kusumaningtyas; Laras Cempaka
Jurnal Teknologi dan Industri Pangan Vol. 33 No. 2 (2022): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.6066/jtip.2022.33.2.178

The traditional cultivation and limited use of bambara groundnut (Vigna subterranea) seed and coat have encouraged the development of this commodity. The aim of this research was to characterize the seed and coat of bambara groundnut from Jampang, Kab. Sukabumi, West Java. Analysis on bambara groundnut seed including proximate analysis, in vitro protein digestibility, starch content, and dietary fiber, as well as analysis on its coat including anthocyanin, total phenolic, antioxidant activity, phytic acid and tannin, were examined in this study. The results showed that bambara groundnut seed from Jampang-Sukabumi contained 16.53% proteins, 3.04% ash, 7.83% fats and 55.22% carbohydrates in dry basis (db). The carbohydrates consisted of starch 52.71% and dietary fiber 7.47% (db). The protein had an in vitro protein digestibility of 41.65% db. The purple seed coat contained of 1.51% anthocyanin, 25.85 mg/g total phenolic content (as gallic acid equivalent), antioxidant activity at 82.75% inhibition of free radical DPPH, 6.37 mg/g phytic acid, and 96.79 mg/g tannin (as tannic acid equivalent) in dry basis. The relatively high content of tannin and antioxidant activity but very low phytic acid content, make the bambara seed coat a potential source for tannin, meanwhile the bambara groundnut is potential as a nutrition source.

Exploration of Potential Actinomycetes from CIFOR Forest Origin as Antimicrobial, Antifungus, and Producing Extracellular Xylanase Sipriyadi Sipriyadi; Yulin Lestari; Aris Tri Wahyudi; Anja Meryandini; Maggy Thenawidjaja Suhartono
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5052

This study aimed to isolate and explore the actinomycetes of CIFOR forest origin as an antimicrobial and antifungal agent, to produce an extracellular xylanase, and to identify isolates based on 16S rRNA gene sequences. Actinomycetes were isolated using Humic-acid Vitamin-B agar (HV) media. Actinomycetes colonies that grow on the medium HV was subsequently purified by growing them on yeast malt agar (YMA) media), then an antagonistic test of selected bacteria against Bacillus sp., Escherichia coli, Fusarium oxysporum, and Sclerotium sp was performed. Xylanase activity test was detected by observing a clear zone, followed by identification. Total of 35 isolates of actinomycetes isolated based on their colony morphology characteristics and diverse types of spore chains showed Streptomyces spp. of isolates CFR-06, CFR-15, CFR-17, CFR-18, and CFR-19 were able to inhibit the growth of Bacillus sp.. The highest inhibition zone has a diameter of 10.1 mm (isolate CFR-17). Isolates CFR-01 and CFR-15 were able to inhibit the growth of E. coli with the highest inhibition zone diameter of 5.1 mm (isolate CFR-15). Isolates CFR-29 and CFR-12 were able to inhibit the growth of F. oxysporum while isolate CFR-35 were able to inhibit the growth of Sclerotium sp.. Xylanase activity test showed that isolates CFR-12, CFR-20, CFR-22, CFR-24, CFR-25, CFR-30, CFR-33, CFR-34 have an ability to produce extracellular xylanase enzyme. Actinomycetes isolate (Xyl_22) as a potential xylanase enzyme producer was closely related with Streptomyces drozdowicii by the maximum similarity of 99%.How to CiteSipriyadi, S., Lestari, Y., Wahyudi, A., Meryandini, A., Suhartono, M. T. (2016). Exploration Potential CIFOR Forest actinomycetes origin as Antimicrobial, Anti Fungus and Producing Enzymes Extracellular Xylanase. Biosaintifika: Journal of Biology Biology Education, 8(1), 94-102.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v40i2.6382

Black pod disease (BPD) of cacao caused by Phytophthora palmivora(Butl.) is one of the major diseases on cacao plantation worldwide. Endophytic fungi (EF), fungi that live asymptomatically inside healthy plants, were examined to study their potentials as biocontrol agent of the disease. Six of EF selected from 37 species (from 2843 isolates), isolated from healthy pods of cacao from Marena in Central Sulawesi were tested for their abilities to control BPD. Pods on living trees in the field were sprayed with each EF propagules. The inoculated pods were harvested two weeks later and subsequently inoculated with P. palmivora. Scoring of disease development was performed and quantified as area under disease progress curve (AUDPC). Latent period, infection rate and effectiveness were also recorded. In vitro growth inhibition of pathogen and induced plant defense mechanisms due to EF were also investigated. Xylariaceaeand Calocybe gambosatreatment generated the highest effectiveness control level, i.e. 38.8% and 33.8% respectively, followed by Resinicium friabileand Aschersoniatreatment, i.e. 17.4% dan 12.7% respectively. Pestalotiopsisand Fusariumwere not effective to control BPD. There was a strong connection between disease severity of BPD with the latent period of pathogen. Growth inhibition of pathogen and induced resistance of plant were partially responsible for disease suppression by Xylariaceae, C. gambosa, R. friabileand Aschersonia. Keywords: Calocybe gambosa, induced resistance, peroxidases, salicylic acid, Xylariaceae

Fecal Lipid Content, Serum Lipid Profile, and Intra-Abdominal Fat Accumulation in Normal Rats Supplemented with Rice Bran Oil Navratilova, Hana Fitria; Damayanthi, Evy; Ardiansyah, Romadhony; Fitriyana, Intan Nur; Soejoedono, Retno Damayanti; Thenawidjaja, Maggy
Jurnal Gizi dan Pangan Vol. 15 No. 2 (2020)
Publisher : The Food and Nutrition Society of Indonesia in collaboration with the Department of Community Nutrition, IPB University

This study aimed to investigate the mechanism of rice bran oil on altering lipid absorption and blood lipid level in normal rat. Male Sprague Dawley rat age 3 months old, weighted 250–300 g were grouped into three groups: control (aquabidest 1 ml), orlistat (2.16 mg / 200 g body weight), and rice bran oil (1.04 mg γ-oryzanol / 200 g body weight). The intervention was given through oral gavage, daily for 28 days. Indicators observed were growth performance, total cholesterol and serum triglyceride levels, lipid levels in feces, and accumulation of intra-abdominal fat. The results showed that the treatment did not significantly affect body weight gain. Fecal lipid levels of orlistat, rice bran oil and control group respectively were 0.19g; 0.17g and 0.13 g (p<0.05), while the percentage of indigestible lipids for orlistat, control and rice bran oil group were 26%, 17% and 13% respectively (p<0.05). Total cholesterol and serum triglyceride levels in rice bran oil group were significantly lower than controls. Rice bran oil did not significantly affect the percentage of total intra-abdominal fat and the weight of the heart and kidney (p>0.05). The intervention of rice bran oil was shown to reduce blood cholesterol and triglyceride levels in normal mice and did not accumulate intra-abdominal fat. The results suggest that rice bran oil might have an effect on blood lipid regulation but not by preventing lipid absorption.

Optimization of Culture Conditions for Production of β-Mannanase by Strain Nonomuraea sp. ID06-379 using Submerged Substrate Fermentation Ratnakomala, Shanti; Yopi, Yopi; Suhartono, Maggy Thenawidjaja; Meryandini, Anja; Prasetya, Bambang
Annales Bogorienses Vol. 18 No. 2 (2014): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

The objective of this study was to investigate the effect of media compositions on the production of β-mannanase by Nonomuraea sp. ID06-379. The study was focused on the influence of carbon, nitrogen, phosphorus and detergents on β-mannanase synthesis through manipulating media compositions on production medium. The results indicated that for carbon sources, locus bean gum (0.745 ± 0.036 U/ml) showed maximum mannanase activity. Malt extract was the best nitrogen source for producing β-mannanase (1.075 ± 0.006 U/ml), (NH4)2HPO4 as phosphate source (1.733 ± 0.026 U/ml) and Tween 80 (1.145 ± 0.003 U/ml) as surfactants effect on increasing permeability of bacterial cell membrane, enhancing membrane transport and excretion of extracellular enzymes into the production media. The results showed that 1% malt extract, 0.5% locus bean gum and 0.05% (NH4)2HPO4 were good substances for nitrogen source, carbon source and phosphate respectively. The highest production of β-mannanase by Nonomuraea sp. ID06-379 (5.33 U/mg) was reached in the medium optimization (Vogel’s minimal medium) contained the following ingredients: 0.5% locus bean gum, 1% malt extract and 0.05% (NH4)2HPO4, under submerged fermentation with shaking at 120 rpm and 28C for 2 days incubation.

Co-Authors , Widodo Abd. Rauf Patong Agnes Imelda Manurung Ahyar Ahmad ALBERTA RIKA PRATIWI Anja Meryandini Anja Meryandini Apon Zaenal Mustopa Apon Zaenal Mustopa Ardiansyah, Romadhony Aris Tri Wahyudi Bambang Prasetya DAHRUL SYAH Dedi Fardiaz Diana Lestari, Diana Diana Nur Afifah Efi Toding Tondok Ekowati Chasanah Elvira Syamsir Endang Yuli Purwani Eni Kusumaningtyas Eni Kusumaningtyas Enny Rimita Sembiring Evy Damayanthi Fitriyana, Intan Nur Fransisca Rungkat Zakaria Fransiska Rungkat Zakaria Fransiska Rungkat Zakaria Fransiska Rungkat Zakaria Haliza, Winda Hana Fitria Navratilova Hanifah Nuryani Lioe Harsi D. Kusumaningrum Hartati Chairunnisa Hasnah Natsir I Gusti Ngurah Kade Mahardika I wayan Teguh Wibawan Joshua Evan Katarina Aninda Karisma Palupi Kusmajadi Suradi Kusmajadi Suradi Laras Cempaka Laurentia Stephani Lilis Nuraida LILY MARIA GORETTI PANGGABEAN LINAWATI HARDJITO Meda Canti Meity Suradji Sinaga Muhammad Hanafi Palupi, Nurheni Sri Purwani, Endang Yuli Puspo Edi Giriwono R Susanti Raphaella Widiastuti Ratih Dewanti -Hariyadi Raymond R. Tjandrawinata Retno Damajanti Soejoedono Retno Damayanti Soejoedono Rinto . Rizki Maryam Astuti Rosmawati Perangiangin Rosmawaty Peranginangin Rosmawaty Peranginangin Rosmawaty Peranginangin Satya Nugroho SEDARNAWATI YASNI Setyani Budiari SHANTI RATNAKOMALA Sherly Valentina Sipriyadi Sipriyadi Sipriyadi Sri Budiarti Poerwanto Sri Sugiwati Subaryono Subaryono Subaryono Subaryono Subaryono Subaryono TATI NURHAYATI Wangsa Tirta Ismaya Wendry Setiyadi Putranto Winda Haliza Yanti Lim YOPI YOPI YULIN LESTARI Yuwanita Ardilasari

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