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Molecular Characterization of a Rigid Rod-Shaped Virus Isolated from Frangipani (Plumeria sp.) Showing Mosaic Symptom in Taiwan Fery Abdul Choliq; Tsang-Hai Chen; Liliek Sulistyowati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1118.483 KB) | DOI: 10.21776/ub.jels.2016.007.01.01

Abstract

Frangipani is an important succulent plant around the worlds and also in Taiwan, for example, Plumeria rubra is widely grown as a popular ornamental tree in parks and landscaped establishments in Taiwan. Recently, a new disease in frangipani with mosaic and distortion symptoms was found in Taiwan. No viruses caused frangipani disease has been reported in Taiwan and the references about frangipani disease are still limited and only Frangipani mosaic virus (FrMV) was found. In this study, the molecular properties of a virus isolated from symptomatic frangipani in south Taiwan, such as Pingtung, Kauhsiung and Tainan were investigated. The virus with rod-shaped particles of 300 nm long and 18 nm in diameter was examined inside diseased leaves by electron microscopy. The purified virus particles showed the typical UV spectrum of tobamoviruses with A260/A280 value of 1.29 and maximum and minimum absorption at 260 nm and 249 nm, respectively. The molecular weight of 19.5 kDa as the size of coat protein of tobamoviruses was estimated by sodium dedocyl sulfate-polyacrylamide gel (SDS-PAGE). Furthermore, the degenerate primers for tobamoviruses were used to amplify 568 bp and 400 bp of the DNA fragments in RT-PCR and nested PCR, respectively. Based on these results, it was confirmed that the rigid rod-shaped virus isolated from mosaic symptom of frangipani leaves is an isolate of FrMV, belonging to the genus Tobamovirus. This is the first report thatFrMV infecting Plumeria sp. in Taiwan.Keywords: Frangipani plant, FrMV, mosaic disease, Tobamovirus.
Effect of Calcium Polysulfide Applications on Severity of Cocoa Pod Rot (Phytophthora palmivora Butl.) and Fungal Diversity Wahyu Widiyasmoro; Mintarto Martosudiro; Liliek Sulistyowati
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1305.655 KB) | DOI: 10.21776/ub.jels.2017.007.02.02

Abstract

Indonesian government gives a high priority for the development and revitalization of cocoa production. In 2012/2013, production of cocoa in Indonesia reached 410,000 ton from 1,774,500 ha (about 231 kg ha-1), lower than Ghana which is 835,000 ton from 1,600,300 ha (about 521 kg ha-1). One of the constraints of cocoa production in Indonesia is pod rot disease caused by Phytophthora palmivora, which can reduce about 90% of production during wet season. Calcium polysulfide was suggested as one of the potential, cheap and save substance to control P. palmivora. The purpose of this study was to evaluate the calcium polysulfide potential to control the disease and its effect on the phyllosphere-fungal diversity. A positive correlation was found between concentration of calcium polysulfide and inhibition of fungal growth in the media. Otherwise, a negative correlation was found between concentration of calcium polysulfide and fungal biomass. Disease severity of cocoa treated by calcium polysulfide 80 mL L-1 every week was 11.67% significantly lower than control, i.e. 68.33%. Furthermore, it was also found that the index value of fungal diversity on the phylosphere decreased by increasing concentration and interval of  calcium polysulfide application.Keywords: Calcium polysulfide, Cocoa, Phytophthora palmivora.
Potency Microbial Unicellular Filoplan Chili and Its Inhibitory to Collectotrichum capsici Causal Antraknose Disease on Chili (Capsicum annuum L.) Tanzil, Ahmad Ilham; Djauhari, Syamsuddin; Sulistyowati, Liliek
Jurnal Online Pertanian Tropik Vol. 7 No. 2 (2020): JURNAL ONLINE PERTANIAN TROPIK
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.572 KB) | DOI: 10.32734/jopt.v7i2.4329

Abstract

This study was conducted to isolate filoplan unicellular microbes which have antibiosis ability and characterize the secondary metabolite produced to inhibit Collectotrichum capsici in-vitro. This research was conducted at the Departement Plant Pest and Diseases, University of Brawijaya. Microbe isolated from the surface of chilies in a different age. Isolates microbes were tested for antagonistic potential against C. capsici. The persistence of the potential antagonist under different conditions of pH, temperature, and the light was also examined. An antagonist test was conducted to confirm the antibiosis mechanism. Furthermore, extracts were characterized using pH, light exposure, and temperature. The most potential antagonist was identified as isolate C3C with 36.20% inhibition. pH 5, 350C temperature, and light exposure of 0% is the best media optimization with the percentage of inhibition 37.5%, 38.2%, and 42.3%.
Association Of Nitrogen-Fixing Bacteria In Rice Muhammad, Muhammad; Sulistyowati, Liliek; Widayati, Wiwik E; Parwito, Parwito
SINTA Journal (Science, Technology, and Agricultural) Vol. 3 No. 2 (2022)
Publisher : Perkumpulan Dosen Muda (PDM) Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37638/sinta.3.2.49-56

Abstract

The research was aimed first: to understandability of GFP marker (green fluorescent protein) gave identities nitrogen-fixing bacteria that clear as endophytic when was the application to plant tissue. The research was aimed second: to understandability of nitrogen-fixing bacteria make special colonization as endophytic use Seed dressing method and Dipping method. The results of the research are GFP markers that show its ability to provide a clear identity as an endophytic bacterium when applied in natural microbial communion. Besides that, GFP is also stable in the applications on rice plant tissue and is able to provide a fairly clear expression. The bacteria Azospirillum lipoferum-1224, Azospirillum lipoferum-1247, Azorhizophilus paspali-2283, Azotobacter chroococum-2286 were able to colonize the rice plant tissue using either the inoculation method of Dipping or Seed dressing.
Study of Expression of Sugarcane Sucrose Transporter cDNA in Yeast Slameto, Slameto; Sugiharto, Bambang; Basuki, Nur; Sulistyowati, Liliek
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.72

Abstract

Studies in transgenic sugarcane (Saccharum officinarum L.) demonstrated that sucrose transporter (SUT) genes were essential in sucrose translocations. Sucrose transporter gene isolated from sugarcane were designated as SoSUT1 and SoSOSUT2 respectively as encode protein of 518 and 747 amino acids. The genes were constructed into plasmid pYES2 for SoSUT1, and pYX112 for SoSOSUT2. cDNA SoSOSUT2 had also constructed into plasmid pBIN-At-GFP which was possible to trace the gene inserted. The constructed plasmid was transformed into yeast (Saccharomyces cerevisiae) and grown in minimal medium (SD–urasil) as selection medium. The transformed colony was confirmed using PCR. The functional expression was studied by growing yeast in YPD medium with 2% Sucrose, then the sucrose uptake was measured in number interval time using resorcinol method. The result showed that yeast INVSc1-pYES2-SoSUT1 and BF264- pYX112-SoSOSUT2 had higher ability in sucrose transport compared to the control-INVSc1 for SoSUT1 and control-BF264 for SoUT2. Moreover, the result showed that SoSUT1 had higher ability to transport sucrose than SoSOSUT2. Confocal microscope observation showed that transformation gene SoSOSUT2 was successful, which was indicated by green exposure of GFP protein.Keyword: sugarcane, sucrose transporter, yeast
𝗘𝗸𝘀𝗽𝗹𝗼𝗿𝗮𝘀𝗶 𝗷𝗮𝗺𝘂𝗿 𝗿𝗶𝘇𝗼𝘀𝗳𝗲𝗿 𝗽𝗮𝗱𝗮 𝘁𝗮𝗻𝗮𝗺𝗮𝗻 𝗽𝗮𝗱𝗶 𝗱𝗮𝗻 𝘂𝗷𝗶 𝗮𝗻𝘁𝗮𝗴𝗼𝗻𝗶𝘀 𝘁𝗲𝗿𝗵𝗮𝗱𝗮𝗽 𝗥𝗵𝗶𝘇𝗼𝗰𝘁𝗼𝗻𝗶𝗮 𝘀𝗼𝗹𝗮𝗻𝗶 𝗽𝗲𝗻𝘆𝗲𝗯𝗮𝗯 𝗽𝗲𝗻𝘆𝗮𝗸𝗶𝘁 𝗵𝗮𝘄𝗮𝗿 𝗽𝗲𝗹𝗲𝗽𝗮𝗵 𝘀𝗲𝗰𝗮𝗿𝗮 𝗶𝗻 𝘃𝗶𝘁𝗿o Rahayu, Esti Dwi; Martosudiro, Mintarto; Muhibbudin, Anton; Sulistyowati, Liliek
AGROMIX Vol 14 No 2 (2023)
Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v14i2.3128

Abstract

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