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All Journal The Journal of Experimental Life Sciences (JELS) Jurnal Penelitian Tanaman Industri Journal of Tropical Plant Protection AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia AGROMIX Jurnal Penelitian Tanaman Industri (Littri) SINTA Journal (Science, Technology, and Agricultural) Berkala Penelitian Hayati PERTANIAN TROPIK Jurnal HPT (Hama Penyakit Tumbuhan)

Liliek Sulistyowati

Department Of Plant Pest And Disease, Faculty Of Agriculture, Brawijaya University

Author-ID : 391539

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Veterinary

Published : 26 Documents Claim Missing Document

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Molecular Characterization of a Rigid Rod-Shaped Virus Isolated from Frangipani (Plumeria sp.) Showing Mosaic Symptom in Taiwan Fery Abdul Choliq; Tsang-Hai Chen; Liliek Sulistyowati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Frangipani is an important succulent plant around the worlds and also in Taiwan, for example, Plumeria rubra is widely grown as a popular ornamental tree in parks and landscaped establishments in Taiwan. Recently, a new disease in frangipani with mosaic and distortion symptoms was found in Taiwan. No viruses caused frangipani disease has been reported in Taiwan and the references about frangipani disease are still limited and only Frangipani mosaic virus (FrMV) was found. In this study, the molecular properties of a virus isolated from symptomatic frangipani in south Taiwan, such as Pingtung, Kauhsiung and Tainan were investigated. The virus with rod-shaped particles of 300 nm long and 18 nm in diameter was examined inside diseased leaves by electron microscopy. The purified virus particles showed the typical UV spectrum of tobamoviruses with A260/A280 value of 1.29 and maximum and minimum absorption at 260 nm and 249 nm, respectively. The molecular weight of 19.5 kDa as the size of coat protein of tobamoviruses was estimated by sodium dedocyl sulfate-polyacrylamide gel (SDS-PAGE). Furthermore, the degenerate primers for tobamoviruses were used to amplify 568 bp and 400 bp of the DNA fragments in RT-PCR and nested PCR, respectively. Based on these results, it was confirmed that the rigid rod-shaped virus isolated from mosaic symptom of frangipani leaves is an isolate of FrMV, belonging to the genus Tobamovirus. This is the first report thatFrMV infecting Plumeria sp. in Taiwan.Keywords: Frangipani plant, FrMV, mosaic disease, Tobamovirus.

Effect of Calcium Polysulfide Applications on Severity of Cocoa Pod Rot (Phytophthora palmivora Butl.) and Fungal Diversity Wahyu Widiyasmoro; Mintarto Martosudiro; Liliek Sulistyowati
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Indonesian government gives a high priority for the development and revitalization of cocoa production. In 2012/2013, production of cocoa in Indonesia reached 410,000 ton from 1,774,500 ha (about 231 kg ha-1), lower than Ghana which is 835,000 ton from 1,600,300 ha (about 521 kg ha-1). One of the constraints of cocoa production in Indonesia is pod rot disease caused by Phytophthora palmivora, which can reduce about 90% of production during wet season. Calcium polysulfide was suggested as one of the potential, cheap and save substance to control P. palmivora. The purpose of this study was to evaluate the calcium polysulfide potential to control the disease and its effect on the phyllosphere-fungal diversity. A positive correlation was found between concentration of calcium polysulfide and inhibition of fungal growth in the media. Otherwise, a negative correlation was found between concentration of calcium polysulfide and fungal biomass. Disease severity of cocoa treated by calcium polysulfide 80 mL L-1 every weekÂ was 11.67% significantly lower than control, i.e. 68.33%. Furthermore, it was also found that the index value of fungal diversity on the phylosphere decreased by increasing concentration and interval ofÂ calcium polysulfide application.Keywords: Calcium polysulfide, Cocoa, Phytophthora palmivora.

Potency Microbial Unicellular Filoplan Chili and Its Inhibitory to Collectotrichum capsici Causal Antraknose Disease on Chili (Capsicum annuum L.) Tanzil, Ahmad Ilham; Djauhari, Syamsuddin; Sulistyowati, Liliek
Jurnal Online Pertanian Tropik Vol. 7 No. 2 (2020): JURNAL ONLINE PERTANIAN TROPIK
Publisher : Talenta Publisher

This study was conducted to isolate filoplan unicellular microbes which have antibiosis ability and characterize the secondary metabolite produced to inhibit Collectotrichum capsici in-vitro. This research was conducted at the Departement Plant Pest and Diseases, University of Brawijaya. Microbe isolated from the surface of chilies in a different age. Isolates microbes were tested for antagonistic potential against C. capsici. The persistence of the potential antagonist under different conditions of pH, temperature, and the light was also examined. An antagonist test was conducted to confirm the antibiosis mechanism. Furthermore, extracts were characterized using pH, light exposure, and temperature. The most potential antagonist was identified as isolate C3C with 36.20% inhibition. pH 5, 350C temperature, and light exposure of 0% is the best media optimization with the percentage of inhibition 37.5%, 38.2%, and 42.3%.

Association Of Nitrogen-Fixing Bacteria In Rice Muhammad, Muhammad; Sulistyowati, Liliek; Widayati, Wiwik E; Parwito, Parwito
SINTA Journal (Science, Technology, and Agricultural) Vol. 3 No. 2 (2022)
Publisher : Perkumpulan Dosen Muda (PDM) Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37638/sinta.3.2.49-56

The research was aimed first: to understandability of GFP marker (green fluorescent protein) gave identities nitrogen-fixing bacteria that clear as endophytic when was the application to plant tissue. The research was aimed second: to understandability of nitrogen-fixing bacteria make special colonization as endophytic use Seed dressing method and Dipping method. The results of the research are GFP markers that show its ability to provide a clear identity as an endophytic bacterium when applied in natural microbial communion. Besides that, GFP is also stable in the applications on rice plant tissue and is able to provide a fairly clear expression. The bacteria Azospirillum lipoferum-1224, Azospirillum lipoferum-1247, Azorhizophilus paspali-2283, Azotobacter chroococum-2286 were able to colonize the rice plant tissue using either the inoculation method of Dipping or Seed dressing.

Study of Expression of Sugarcane Sucrose Transporter cDNA in Yeast Slameto, Slameto; Sugiharto, Bambang; Basuki, Nur; Sulistyowati, Liliek
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.72

Studies in transgenic sugarcane (Saccharum officinarum L.) demonstrated that sucrose transporter (SUT) genes were essential in sucrose translocations. Sucrose transporter gene isolated from sugarcane were designated as SoSUT1 and SoSOSUT2 respectively as encode protein of 518 and 747 amino acids. The genes were constructed into plasmid pYES2 for SoSUT1, and pYX112 for SoSOSUT2. cDNA SoSOSUT2 had also constructed into plasmid pBIN-At-GFP which was possible to trace the gene inserted. The constructed plasmid was transformed into yeast (Saccharomyces cerevisiae) and grown in minimal medium (SD–urasil) as selection medium. The transformed colony was confirmed using PCR. The functional expression was studied by growing yeast in YPD medium with 2% Sucrose, then the sucrose uptake was measured in number interval time using resorcinol method. The result showed that yeast INVSc1-pYES2-SoSUT1 and BF264- pYX112-SoSOSUT2 had higher ability in sucrose transport compared to the control-INVSc1 for SoSUT1 and control-BF264 for SoUT2. Moreover, the result showed that SoSUT1 had higher ability to transport sucrose than SoSOSUT2. Confocal microscope observation showed that transformation gene SoSOSUT2 was successful, which was indicated by green exposure of GFP protein.Keyword: sugarcane, sucrose transporter, yeast

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AGROMIX Vol 14 No 2 (2023)
Publisher : Fakultas Pertanian Universitas Yudharta Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/agx.v14i2.3128

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Co-Authors Abdul Cholil Abdul Cholil Abdul Cholil Abdul Cholil Abdul Latief Abadi Ahmad Ilham Tanzil Alfi Inayati Anggraeni Eka Puspitasari Anggraeni Eka Puspitasari Anton Muhibbudin Anton Muhibuddin Anton Muhibuddin Ari Kristini Arif Wibowo Aulanni ‘am Aulanni ‘am Bagyo Yanuwiadi Bambang Sugiharto Birtha Niken Pratiwi C Martasari C Martasari Chiu-Chsiung Cheng Christanti Sumardiyono Dharmawan Putra Dharmawan Putra Dhewyangga Bismi Panglipur Dhewyangga Bismi Panglipur Dian Wulandari Djanggan Sargowo Eriyanto Yusnawan Fery Abdul Choliq Hanis Alifatuz Zakiyah I GEDE PUTU WIRAWAN I Ketut Muliartha Intan Sugiarti Permatasari Kusuma, Restu Rizkyta Luqman Qurata Aini MADE SRITAMIN Mintarto Martosudiro Mintarto Martosudiro Muhammad Akhid Syibli Muhammad Muhammad Muizzuddin Muizzuddin Nilasari Martha Dewi Nilasari Martha Dewi Nur Basuki Nurul Hidayah NURUL HIDAYAH Nurul Umayatul Aliah Pandu Indra Pratama Parwito Parwito, Parwito Peter Taylor Rahayu, Esti Dwi Rizatul Maela Tri Intan Rizatul Maela Tri Intan Rosy Husna Shofiana RULLY DYAH PURWATI Siti Subandiyah Slameto Slameto Soleudin Efendi Sri Karindah Syamsuddin Djauhari Tinny Endang H. Tsang Hai Chen Tsang-Hai Chen Wahyu Widiyasmoro Widayati, Wiwik E Wita Firdausi Yi-Hsien Lin Yo-Chia Chen Yohana Avelia Sandy Yunita Dian Puspita Yuricha Kusumawardani Zevita Yunade Ganda Tirtana Zevita Yunade Ganda Tirtana

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