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VALIDASI E-MODUL BERBASIS PENELITIAN HEMATOLOGI Rattus novergicus Crescentia Mishi Juniorita Ngangi; Jantje Ngangi; Emma Mauren Moko; Herry Maurits Sumampouw; Meity Neltje Tanor; Dino Rahardiyan; Livana Dethris Rawung
Jambura Edu Biosfer Journal Vol 5, No 1 (2023): Jambura Edu Biosfer Journal
Publisher : Fakultas MIPA Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34312/jebj.v5i1.17866

Abstract

Pengembangan dan pemanfaatan media sebagai sumber belajar dalam pembelajaran memiliki korelasi yang sangat erat dengan teknologi sehingga perlu untuk selalu meningkatkan kualitas pembelajaran. Kehadiran konsep Merdeka Belajar Kampus Merdeka memunculkan paradigma baru dalam dunia pendidikan. Salah satu perubahan berdasarkan kebijakan kurikulum merdeka di sekolah adalah perbaikan RPP yang dikemas dalam bentuk RPP Plus yang dikembangkan dalam bentuk modul pembelajaran yang berisi desain dan materi pembelajaran. Penelitian ini bertujuan untuk mengembangkan media pembelajaran biologi modul digital (e-modul) berupa RPP plus berbasis penelitian hematologi pada hewan coba Rattus novergicus dalam menunjang implementasi kurikulum merdeka di SMA. Dalam penelitian ini, jenis penelitian RD model Kemp Dayton yang sebelumnya memiliki 9 langkah dirampingkan ke dalam 4 langkah utama, yaitu 1) persiapan, 2) desain, 3) pengembangan dan 4) uji validitas. Pengembangan modul digitak atau e-modul dibatasi pada uji validitas yang melibatkan validator ahli media dan ahli materi. Hasil validasi oleh ahli media dan ahli materi pembelajaran menunjukkan persentase keseluruhan sebesar 89,3% dan 100%. Media pembelajaran yang dikembangkan sangat berkualitas, layak dan tidak perlu direvisi
Uji Efektivitas Ekstrak Daun Pepaya (Carica papaya L.) Sebagai Insektisida Alami Lalat Rumah (Musca domestica) Melany Piri; Herry M Sumampouw; Emma Mauren Moko; Decky W Kamagi; Helen Lawalata
JURNAL BIOS LOGOS Vol. 12 No. 2 (2022): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v12i2.41034

Abstract

The house fly (Musca domestica) is a vector of mechanical transmission of infectious diseases such as diarrhea, dysentery, cholera, typhoid. One way to control house flies is to use plant-based insecticides. Papaya leaves (Carica papaya L.) can be used as insecticides because of the enzymes saponins, flavonoids, and papain which are toxic to insects. This research aims to determine the effectiveness of papaya leaf extract as a natural insecticide for house flies. The concentration of papaya leaf extract used was 0%, 30%, 40%, 50%, and 60% with 3 replications. Each treatment unit contained 10 house flies. Extract application by spraying directly on house flies. The number of house fly deaths was calculated at 1, 2, 3, 4, 5, 6 and 24 hours. Data were analyzed using One Way Anova test. The result of this research was all the sig values are 0.00 < 0.05, which means that there was a significant difference in the number of house flies deaths at each concentration. In conclusion, the most effective concentration of papaya leaf extract to kill 100% of house flies was at a concentration of 60% after 6 hours of treatment.Keywords: house fly; natural insecticide; papaya leaf ABSTRAKSalah satu vektor penularan penyakit menular mekanis seperti diare, disentri, kolera, tipes adalah lalat rumah (Musca domestica). Salah satu cara pengendalian lalat rumah adalah menggunakan insektisida nabati. Daun pepaya (Carica papaya L.) mengandung enzim saponin, flavonoid, dan papain yang bersifat racun bagi serangga sehingga dapat digunakan sebagai insektisida alami atau insektisida nabati. Tujuan dari penelitian ini adalah untuk mengetahui efektivitas ekstrak daun pepaya sebagai insektisida nabati lalat rumah menggunakan 5 perlakuan konsentrasi ekstrak kasar daun pepaya yakni 0%, 30%, 40%, 50%, dan 60% serta 3 ulangan, setiap unit perlakuan menggunakan 10 ekor lalat rumah, aplikasi ekstrak kasar daun pepaya dengan cara disemprotkan pada lalat rumah, jumlah kematian lalat rumah dihitung pada jam ke-1,2,3,4,5,6 dan 24 jam setelah aplikasi. Data dianalisis menggunakan uji One Way Anova. Hasil penelitian diperoleh semua nilai sig 0,00 < 0,05 yang berarti terdapat perbedaan bermakna jumlah kematian lalat pada setiap pemberian konsentrasi ekstrak daun pepaya. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak daun pepaya yang paling efektif untuk membunuh 100% lalat rumah adalah konsentrasi 60% setelah 6 jam perlakuan.Kata kunci: daun pepaya; insektisida alami; lalat rumah
Penerapan Pendekatan Multi Representasi Terhadap Kemampuan Metakognitif Materi Sistem Reproduksi Manusia SMP Negeri 2 Halut Sri Kevin S. Alaratu; Herry M. Sumampouw; Jovialine Albertine Rungkat
SCIENING : Science Learning Journal Vol. 4 No. 2: Desember 2023
Publisher : Jurusan Pendidikan IPA FMIPA Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/slj.v4i2.2842

Abstract

Tujuan dalam penelitian ini adalah untuk meningkatkan kemampuan Metakognitif peserta didik materi sistem reproduksi pada manusia dengan penerapan pendekatan multi representasi di SMP Negeri 2 Halmahera Utara (HALUT). Subjek penelitian ini berjumlah 21 siswa yang terdiri dari 11 siswa laki-laki dan 10 siswa perempuan. Penelitian ini merupakan jenis penelitian tindakan kelas (PTK). Metode pengumpulan data dalam penelitian ini menggunakan Perencanaan, Pelaksanaan, Observasi, dan Refleksi. Hasil penelitian menunjukan bahwa hasil belajar peserta didik rata-rata pada siklus II adalah 86,67 dengan ketuntasan belajar 88%. Hasil observasi aktivitas pesrta didik dan guru pada siklus II berada pada kategori sangat baik dengn rata-rata skor masing-masing sebesar 3,8 dan 4,0. Hasil tersebut telah mencapai indikator keberhasilan (KKM ≥ 70) sehingga penelitian tindakan dihentikan, sampai pada siklus II. Dari hasil penelitian membuktikan bahwa dengan penerapan pendekatan multi representasi terhadap kemampuan metakongnitif peserta didik kelas VIII SMP Negeri 2 Halmahera Utara.
THE BIOACTIVE CONTENT AND ANTIOXIDANT ACTIVITY OF HONEY BEEHIVES (Apis dorsata BINGHAMI) FROM THE BOGANI NANI WARTABONE BOLAANG MONGONDOW NATIONAL PLANT Yermia S Mokosuli; Herry Sumampouw; Hendra Pratama Maliangkay; Jimmy Langi
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v2i1.4930

Abstract

Bee Honey (Apis dorsata Binghami) Is a subspecies of A. dorsata which is only found in sulawesidan island and surrounding islands and until now has not been successfully cultivated. Various studies have been conducted species of trees used for nesting places, nesting behavior, biotic conditions, physical surrounding nesting trees and nesting characteristics. This study aims to obtain secondary metabolite components and scientific data of antioxidant activity of honeybee extract (Apis dorsata Binghami) derived from Bogani Nani Wartabone Bolaang Mongondow National Park, North Sulawesi Province. The method of extraction in this research was conducted using maceration with methanol solvent, phytochemical test and antioxidant activity analysis using method (1,1-definil-2-picrilhidrazil) by measuring absorbance absorption at maximum wavelength using UV-Vis spectrophotometer. The results of the phytochemical test show that the honeycomb (Apis dorsata Binghami) contains alkaloids, flavonoids, saponins and tannins. From the analysis results showed that with the increasing concentration of extracts, the greater the antioxidant activity is marked by decreasing the value of IC50. The honeycomb (Apis dorsata Binghami) has IC50 of 212.42 ppm in comparison with vitamin C IC50 of 59.34 ppm
ANTIBACTERIAL ACTIVITY OF CHLOROFORM EXTRACT AND RHIZOPHORA SPP LEAVES METHANOL EXTRACT ON MOUTH BACTERIA Carolin Manuahe; Herry Sumampouw; Friska Montolalu
Indonesian Biodiversity Journal Vol. 2 No. 3 (2021): Desember 2021
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v2i3.4987

Abstract

The purpose of this study was to obtain the phytochemical profile of the methanol and chloroform extracts of the leaves of Rhizophora apiculata and to find the antibacterial activity of the methanol and chloroform extracts against human oral bacteria. This research was carried out in 3 stages, namely Rhizopora spp. Leaf Sampling, Extraction and Analysis of Secondary Metabolite Content and Antibacterial Activity Test using the agar diffusion method. Based on the results of the phytochemical screening examination of the leaf extract of Rhizopora spp., the results showed that it was rich in chemical compounds of alkaloids, flavonoids, tannins, and saponins in both methanol extract and chloroform extract. The results of the antibacterial activity test of methanol extract obtained that the smallest concentration in bacterial isolate bacteria 2 was 30 mg/ml, while the smallest concentration in bacterial isolate 1 was 50 mg/ml. The results of the antibacterial activity test of the chloroform extract obtained that the smallest concentration in the bacterial isolate bacteria 2 was 20 mg/ml while the smallest concentration in the bacterial isolate 1 was 30 mg/ml. Both methanol extract and chloroform extract had better activity on gram-positive bacteria.
Ration Formulation of Native Chicken from Fruit Fly (Drosophila melanogaster L.) and Water Hyacinth Flour (Eichornia crasipess (Mart.) Solms) Febiola Najoan; Herry Sumampouw; Yermia Mokosuli
Indonesian Biodiversity Journal Vol. 3 No. 3 (2022): December 2022
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v3i3.5723

Abstract

Fruit flies and water hyacinths are believed to be able to increase body weight in native chickens because they have a relatively high protein content. This study aims to determine the effect and dose of fruit fly (Drosophila melanogaster L.) with a combination of water hyacinth flour (Eichornia crassipes (Mart.) Solms) for consumption and body weight gain in native chickens (Gallus domesticus L.). The method used in this study used a Completely Randomized Design (CRD) method. Where this study was an experimental study using five treatments and four replications. The results showed that the average consumption of free-range chicken rations was 30.41 g per head per week, where P1 was the highest ration consumption at 6.75 g per head per week, and the lowest was at P4 at 5.72 g per head per week. On body weight gain, native chickens had an average of 11.42 g per head per week, where P2 had the highest average of 2.39 g per head per week and P1 with the lowest average of 2.13 grams per week. The consumption of free-range chicken rations tends to increase along with the composition of water hyacinth in the ration to 7.5%.
THE EFFECT OF INTEGRATION OF PROBLEM BASED LEARNING (PBL) LEARNING MODELS AND ORIENTATION, IDENTIFY, DISCUSSION, DECISSION AND ENGAGE IN BEHAVIOR (OIDDE) TO SOCIO-SCIENTIFIC DECISION MAKING SKILLS STUDENTS AT SMP NEGERI 2 BITUNG Bellatrix H.Ch Kaliboto; Herry Sumampouw; Ferny M. Tumbel
Journal of Higher Education and Academic Advancement Vol. 1 No. 7 (2024): Journal of Higher Education and Academic Advancement
Publisher : PT ANTIS INTERNATIONAL PUBLISHER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61796/ejheaa.v1i7.709

Abstract

This research uses a hypothesis test of one-way analysis of covariance or one-way ANCOVA to measure the respective effects of using the integration of the PBL model with OIDDE on socioscientific decision making skills. Before testing the hypothesis, prerequisite tests must be carried out, namely the normality test and homogeneity test. The use of this test is assisted by SPSS 23 for Windows software. The conclusion of this research is that the integration of the Problem Based Learning (PBL) learning model with the OIDDE (Orientation, Identify, Discussion, Decision, and Engage In Behavior) learning model has a real influence on improving students' Socioscientific Decision Making skills with a sig. 0.001 > 0.005 and the highest corrected mean value was 86.21
Bioactive Contents, BSLT Toxicity and antioxidants from Apis dorsata Binghami and Apis mellifera nest extracts Tuegeh, Reinny Silvana; Sumampouw, Herry Maurits; Tanor, Meity Neltje; Mokosuli, Yermia Semuel
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4514

Abstract

Beenest is rich in secondary metabolites because honeycombs, among others, are formed from plant resins (propolis). This study aimed to analyze differences in flavonoid content and in vitro antioxidant activity of Apis dorsata Binghami and Apis mellifera nest extracts. The samples used were A. dorsata from North Sulawesi and A. mellifera from South Sulawesi. The honeycomb was extracted using 95% ethanol solvent. Honeycomb extract was analyzed for its flavonoid content by the HPLC method, toxicity was tested by the BSLT method, and in vitro antioxidant activity was tested by the DPPH method. The results of the analysis of flavonoids showed that A. dorsata nest extract produced 21 types of compounds while A. mellifera produced 26 types of compounds. The toxicity test results showed that the A. dorsata nest extract had a better LC50 of 245,691 mg/l than the A. mellifera nest extract with an LC50 of 443,701 mg/l. The in vitro antioxidant test results of A. dorsata nest extract were more robust, namely IC 50 1.161 mg/l, compared to A. mellifera nest extract IC 50 2.404 mg/l. However, both were included in the category of powerful antioxidants. In vitro, anticancer test results on MCF-7 cells, A. mellifera nest extract was active with IC 50 100.02mg/l. Compared to A. dorsata extract, it was active with IC 50 102.217mg/l, but the two were not significantly different. Based on the analysis of flavonoid content, toxicity test, and antioxidant test, A.dorsata and A.mellifera honeycomb extracts have potential as in vitro antioxidants
Isolasi dan Identifikasi Bakteri Escherichia Coli Jajanan Pasar Girian Kota Bitung Marsanda Tandiapa; Helen J. Lawalata; Anita C. Tengker; Herry M. Sumampouw; Wiesye M. S. Nangoy
J-CEKI : Jurnal Cendekia Ilmiah Vol. 3 No. 4: Juni 2024
Publisher : CV. ULIL ALBAB CORP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56799/jceki.v3i4.3746

Abstract

Jajanan (street food) merupakan makanan dan minuman yang dibuat oleh pedagang kaki lima dan dijual di sekolah, pasar dan tempat-tempat umum lainnya. Makanan dan minuman menjadi aspek penting dalam kehidupan manusia. Bakteri Escherichia coli merupakan salah satu bakteri yang ada dalam makanan dan minuman. Adapun tujuan dari penelitian ini yaitu untuk mengisolasi dan mengidentifikasi bakteri Escherichia coli jajanan Pasar Girian Kota Bitung. Metode yang digunakan dalam penelitian ini yaitu metode deskriptif kualitatif. Sampel yang digunakan sebanyak 3 sampel jajanan (minuman boba, kue pukis dan molen), masing-masing sampel diambil dari tempat dan penjual yang berbeda di Pasar Girian. Pengujian dilakukan menggunakan metode Most Probable Number (MPN) seri 5-5-5 dengan 3 pengenceran (). Sampel di ujikan ke dalam media Lauryl Tryptose Broth (LTB) kemudian dilanjutkan ke media Ec Broth, dan kemudian dilakukan penanaman bakteri pada media Eosin Metyle Blue Agar (EMBA), dilakukan pewarnaan gram pada setiap sampel positif dan uji biokimia. Hasil dalam penelitian ini menunjukan bahwa ke tiga sampel yang diperdagangkan di Pasar Girian Kota Bitung positif terkontaminasi oleh bakteri Escherichia coli. Jumlah cemaran terbesar terdapat dalam sampel minuman boba yaitu sebesar >160 MPN/gr.
Studi Deskriptif tentang Tingkat Pemahaman Konsep Keanekaragaman Hayati dan Implikasinya dalam Pembelajaran Biologi di SMA Negeri 1 Pineleng Akulina Kelitubun; M. Herry Sumampouw; Nova L. I. M. Ogi
Jurnal Pendidikan Kimia, Fisika dan Biologi Vol. 1 No. 4 (2025): Juli: Jurnal Pendidikan Kimia, Fisika dan Biologi
Publisher : Asosiasi Riset Ilmu Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61132/jupenkifb.v1i4.419

Abstract

Conceptual understanding is an important aspect of learning biology because it reflects the extent to which students can internalize knowledge and relate it to real life. This study aims to analyse the level of understanding of the concept of biodiversity of students and identify supporting and inhibiting factors in the biology learning process. The research was conducted in class X of SMA Negeri 1 Pineleng with a descriptive qualitative approach. Data was collected through observation, written tests, questionnaires, teacher interviews, and documentation. Conceptual comprehension is measured using three main indicators: the ability to restate concepts, classify objects based on certain properties, and provide examples rather than definitions. The results showed that 87.5% of students had achieved a good level of understanding of classification indicators and giving examples, but only 50% were able to restate concepts completely. The supporting factors identified include learning motivation, parental mentoring, teacher learning strategies, and student absorption. On the other hand, barriers to understanding include lack of enthusiasm for learning, an unsupportive environment, and a lack of parental involvement. These findings confirm that the success of conceptual understanding is determined not only by cognitive aspects but also by comprehensive pedagogical and social support. This research makes an important contribution to the development of more contextual and participatory biology learning strategies, as well as opens opportunities for further research that explores the use of innovative media in strengthening conceptual understanding.