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All Journal HAYATI Journal of Biosciences Indonesian Journal of Pharmaceutical Science and Technology Indonesian Journal of Biotechnology Warta IHP (Warta Industri Hasil Pertanian) Indonesian Journal of Chemistry JFIOnline Jurnal Ilmu Kefarmasian Indonesia Jurnal Aisyah : Jurnal Ilmu Kesehatan Jurnal Locus Penelitian dan Pengabdian Journal of Applied Agricultural Science and Technology Pharmaceutical Sciences and Research (PSR) Eduvest - Journal of Universal Studies Ezra Science Bulletin Jurnal Kefarmasian Indonesia
Herman Suryadi
Department Of Pharmacy, Faculty Of Pharmacy, Universitas Indonesia, Jakarta, Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Electrical & Electronics Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Antibacterial Synergy Detection of Lignin Extract from Oil Palm Empty Fruit Bunches (Opefb) Combined with Amoxicillin Against Staphylococcus Aureus Using The Azdast Method Hendri, Rifki Anshory; Suryadi, Herman; Hayun, Hayun
Journal of Applied Agricultural Science and Technology Vol. 8 No. 1 (2024): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v8i1.191

Abstract

Oil palm empty fruit bunches (OPEFB) are waste generated by the palm oil industry and are generally considered an environmental problem due to their large quantity and difficult recyclability. This study's objective was to examine the potential of OPEFB lignin extract combined with amoxicillin in inhibiting the growth of Staphylococcus aureus bacteria. The Synergism Test of the OPEFB lignin extract-antibiotic combination was conducted using the AZDAST method (Ameri Ziaei Double Antibiotic Synergism Test). The lignin extraction process was a steam process using a 4% oxalic acid solution under optimal conditions. Then, Calcium ions precipitate 4% oxalate by adding Ca(OH)2 to the extract. The Lignin content was determined by UV-Vis spectrophotometry at 280 nm, and then the lignin extract was combined with antibiotics for antibacterial assay. The results showed a lignin content of 1.06 %. The AZDAST test results revealed clear zones from the combination of OPEFB lignin extract and amoxicillin with a diameter of 20.23 mm for the extract containing oxalate and 15.83 mm for the oxalate-free extract. Meanwhile, on average, the clear zone of single amoxicillin was only 14.13 mm. Based on these results, the combination of OPEFB lignin extract with amoxicillin was stronger than amoxicillin alone in inhibiting the growth of S. aureus, but it was not significantly different (p-value> 0.05). In conclusion, the potential of OPEFB lignin extract synergistically worked with the antibiotic amoxicillin in inhibiting the growth of S. aureus.
Antibacterial Potential of Cinnamomum culilaban Bark Ethanolic Extract Prepared by Ultrasound-Assisted Extraction against Oral Pathogens Kurniatri, Arifayu Addiena; Elya, Berna; Suryadi, Herman
Jurnal Kefarmasian Indonesia VOLUME 14, NUMBER 2, AUGUST 2024
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

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Abstract

Cinnamomum culilaban is one of the native Indonesian plants that has been used as medicinal plant. The local community on Seram Island uses the stem bark to treat toothaches. Eugenol, a chemical compound found in Cinnamomum culilaban, is used in dental practice and has antibacterial activity. Research on Cinnamomum culilaban is very limited, its activity against bacteria that cause oral infections has not been reported. This study aims to investigate the antibacterial activity of Cinnamomum culilaban bark extract against oral pathogens Porphyromonas gingivalis, Streptpcoccus mutans, and Enterococcus faecalis. The extraction was carried out using 96% ethanol with ultrasound-assisted extraction method. Disc diffusion assay was conducted to investigate the antibacterial activity. The concentration of extract used were 50%, 25%, and 12.5% (b/v) in DMSO. Eugenol (50% v/v in DMSO) was used as positive control. The phytochemicals screening was carried out to investigate the chemical compounds contained in Cinnamomum culilaban bark extract. Extraction of Cinnamomum culilaban bark using 96% ethanol with the ultrasound-assisted extraction method obtained a yield of 23.36±0.49%. The extract contains alkaloids, flavonoids, tannins, saponins, phenolics, and steroids/terpenoids. The ethanolic extract of Cinnamomum culilaban bark has inhibitory activity against Streptpcoccus mutans and Enterococcus faecalis. Cinnamomum culilaban bark has the potential as an antibacterial agent.
Co-Authors -, Harmita . Azizahwati . Azizahwati . Harmita . Hayun Abdul Mun'ím Agus Firmansyah Ambarsari, Christy Andi Utama ANDI UTAMA Andiri Niza Syarifah Anja Tamabri Arif Arrahman Arif Arrahman Arif Arrahman, Arif Arifayu Addiena Kurniatri Arifianti, Ayun Erwina Arry Yanuar Berna Elya Callista Andinie Mulyadi Chairunissa, Ananda Hanny Dessy Ariyanti Dessy Ariyanti Esti Mumpuni Forestrania, Roshamur Cahyan Fready SIP Fuad, Asrul Muhammad Hayun . Hayun Hayun Hayun Hayun, Hayun Hendri, Rifki Anshory Heria Oktavia Heria Oktavia, Heria Irianti, Marina Ika Jantan, Ibrahim Junaidi, Lukman Kamaruzzaman, Mohd Amir Larosa, Febri LS Lina Elfita Lina Elfita Luh Putu Ratna Sundari Makau, Juliann Nzembi Maksum Radji Maksum Radji Maksum Radji MARIA BINTANG Maryati Kurniadi Maryati Kurniadi Melanie, Yuanki Nafi’ah, Nadya Ismi Nani Dharmasetiawani Ofa Suzanti Betha Partomuan Simanjuntak Prasetio, Nisrina Putri PUSPITA LISDIYANTI Putri Mulia, Ocha Rahmadi, Agus Rahmasari, Ratika Sembiring, Enny Rimita Septiarini, Tri Hastuti Shanti Ratnakomala SHANTI RATNAKOMALA Siburian, Eva Siswa Setyahadi Siswa Setyahadi Sri Wardatun Subagja ., Subagja Sunarsih Sunarsih Susandra Jaya Vicha Vicha Yahdiana Harahap Yahdiana Harahap Yodifta Astiningrum Yodifta Astriningrum Yodifta Astriningrum, Yodifta