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Analisis Kandungan Ion Flourida pada Sampel Air Tanah dan Air PAM Secara Spektrofotometri Astriningrum, Yodifta; Suryadi, Herman; Azizahwati, Azizahwati
Majalah Ilmu Kefarmasian Vol. 7, No. 3
Publisher : UI Scholars Hub

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Abstract

Fluoride ion is one of the compounds that are known to have benefits in the prevention of dental caries when used in certain concentrations, but also hasnegative effects that may cause the occurrence of dental and bone fluorosis when the intake was in excessive concentration. One of the fluoride intakes comes fromwater that is consumed. The aim of this research was to identify and measurefluoride ion levels in groundwater and piped water that used as drinking water consumption in the community. Measurement of fluoride ion concentration isdone by using visible spectrophotometry at the maximum wavelength of 586 nmusing the sodium 2-parasulfophenylazo 1,8-dihydroxy-naphthalene-3,6disulfonate (SPADNS)-zirconil acid reagent. This method was optimized by thesearch of range of absorption which stable for 10 minutes after reagent addition.The limit of detection, limit of quantitation, and coefficient of variation forfluoride ion were 0.0452 mg/L, 0.1506 mg/L, and 0,63%, respectively. While therecovery of fluoride ion in sample were in the range of 90,50-102,04%. The measurement results of the samples showed levels of fluoride ions in groundwaterand piped water varied between 0.05 to 0.78 mg/L. This range was still within allowed levels according the rules of Indonesian health ministers No.492/MENKES/PER/IV/2010 where the maximum allowable fluoride concentration is 1.5 mg/L.
Inhibitory and Anti-Biofilm Effects of Orthoshipon aristatus Against Candida albicans Rahmasari, Ratika; Chairunissa, Ananda Hanny; Irianti, Marina Ika; Forestrania, Roshamur Cahyan; Arifianti, Ayun Erwina; Suryadi, Herman; Makau, Juliann Nzembi; Jantan, Ibrahim; Elya, Berna
Pharmaceutical Sciences and Research Vol. 7, No. 3
Publisher : UI Scholars Hub

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Abstract

There are increasing number of reports on Candida albicans developing resistance to available anti-fungal drugs. Thus, there is an urgent need to discover new agents for treatment of candidiasis. The alcoholic extracts of Orthoshipon aristatus have been shown to exhibit antifungal activity against C. albicans by using the agar diffusion and broth microdilution methods. However, the underlying mechanisms of anti-C. albicans effect of O. aristatus have not been well understood. This study was aimed to evaluate the cytotoxic and anti-biofilm effects of the n-hexane and ethanol extracts of purple and white varieties of O. aristatus leaves and branches against C. albicans. The effect of n-hexane and ethanol extract against C. albicans growth was carried out by crystal violet viability assay. IC50 values of the most active extract, and nystatin and fluconazole as positive controls were also determined by the crystal violet assay. Evaluation of the anti-biofilm effect was performed by treating C. albicans with the extracts at adhesion, development, and biofilm maturation stages using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The n-hexane extract of the purple variety of O. aristatus leaves demonstrated the strongest cytotoxic activity against C. albicans amongst the tested extracts, with an IC50 value of 0.67 µg/mL. The extract also showed strong anti-biofilm effect as fluconazole, with pronounced inhibition at the adhesion stage and less activity at the biofilm development and maturation stages. These results suggested that the n-hexane extract of the purple variety of O. aristatus leaves could be explored for discovery and development of anti-C. albicans agent.
Uji Aktivitas Antibakteri 1-[(Kuinazolin-4-on-2-il)metil]piridin-1-ium Bromida dan 2-Bromometilkuinazolin-4-on Hayun, Hayun; Arrahman, Arif; Suryadi, Herman; Yanuar, Arry
Pharmaceutical Sciences and Research Vol. 1, No. 1
Publisher : UI Scholars Hub

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Abstract

Antibacterial activity tests of quinazolin-4-one derivates: 1-[(6-nitroquinazolin-4one-2-yl)methyl]piridin-1-ium bromide (1), 1-[(6-aminoquinazolin-4-on-2-yl)methyl] piridin-1-um bromide (2), 2-bromomethylquinazolin-4-one (3) and 2-bromomethyl-6nitroquinazolin-4-one(4) with trimethoprim (5) as a positive standard were conducted. The antibacterial activity tests were carried out using disc diffusion method againts E.coli, S.aureus and S.thyposa, and determination of minimum inhibitory concentration (MIC). The results showed that compounds 1 and 2 are inactive as antibacterial, whereas compounds 3 and 4 are active.The activities to E.coli were ¼ times the activity of trimethoprim (MIC : 5 x 102 µg/ml compared to 1.25 x 102 µg/ml ). The activity to S. typhosa were ½-1 times the activity of trimethoprim (MIC: 2.5 and 1.25x102 µg/ml compared to 1.25x102 µg/ml); but they are not active to S. aureus. Trimethoprim active to S. aureus with MIC : 0.62 x102 µg/ ml.
Total Phenolic Content of Natural Deep Eutectic Solvent (NADES) Extraction in Comfrey (Symphytum officinale) Andiri Niza Syarifah; Herman Suryadi; Abdul Mun'im
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 7, No 3: September 2022
Publisher : Universitas Aisyah Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (605.899 KB) | DOI: 10.30604/jika.v7i3.789

Abstract

Objective: The most common type of phytochemical is phenolic compounds. These compounds are mostly found in the plant kingdom, and extraction is one of the first and most important stages before any use. The green chemistry trend has encouraged the researcher to discover and generate innovative solvents in the extraction of phenolic compound (PC) as a strategy to toxic and potentially hazardous organic solvents. Natural Deep Eutectic Solvents (NADES) have recently been used as phenolic compound extraction solvents. The objective of this study is to determine the total phenolic contents of the NADES extract from comfrey (Symphytum officinale). Methods: This study used choline chloride-urea, choline chloride-glycerol and choline chloride-sucrose combination of NADES for extraction of phenolic compound in comfrey. The extraction was helped using Ultrasound Assisted Extraction (UAE). The extracted material was examined using a UV-Vis spectrophotometer. Results: The results showed that the total phenol of choline chloride-urea, chloride-glycerol and choline chloride-sucrose NADES comfrey extraction was 2.069, 1.566, and 1.906 mg GAE /gram of dry powder, respectively. Conclusion: Based on the result, it can be concluded type of NADES combination showed a different level of phenolic extraction. The best phenolic extraction was shown in the combination of choline chloride-urea. Abstrak: Latar belakang: Fenol adalah senyawa kimia yang paling umum ditemukan dalam tanaman. Proses ekstraksi berperan penting dalam pemisahan senyawa fenol dari tanaman sebelum dimanfaatkan. Saat ini penelitian banyak difokuskan pada proses ekstraksi yang ramah lingkungan. Salah satunya adalah menggunakan Natural Deep Eutectic Solvent (NADES) dalam proses ekstraksi senyawa fenol. Tujuan penelitian ini adalah menghitung kadar fenol total dalam ekstrak NADES tanaman komfrey. Metode: Penelitian ini menggunakan NADES yang berasal dari kolin klorida-gliserol, kolin klorida-urea dan kolin klorida-sukrosa. Proses ekstraksi menggunakan Ultrasound Assisted Extraction (UAE) untuk mempercepat proses ekstraksi. Penentuan kadar fenol total diukur menggunakan spectrophotometer UV-Vis. Hasil: Kadar fenol total dalam ekstrak NADES masing-masing adalah 1.566, 2.069 and 1.906 mg GAE /gram sampel. Kesimpulan: Berdasarkan hasil yang diperoleh dapat disimpulkan bahwa kombinasi masing-masing NADES memberikan kemampuan ekstraksi senyawa fenol yang berbeda-beda. Kadar fenol total terbaik dihasilkan oleh kombinasi NADES kolin klorida-urea. 
Production of Anti-Recombinant Human Insulin Antibody and Validation by Indirect ELISA Ambarsari, Christy; Suryadi, Herman; Yanuar, Arry
Indonesian Journal of Pharmaceutical Science and Technology Vol 10, No 3 (2023)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v10i3.40641

Abstract

Human insulin potential has become an interest and is important in maintaining the success of therapyin patients with the availability of chemical-based analytical methods, however, only a few have beenusing immunoassays. This study aimed to produce IgG polyclonal antibodies from rabbits immunizedwith 1 mg/mL rhINS subcutaneously and validated by indirect ELISA. Antibody was precipitated andfractioned on a HiTrap® Protein A HP column before being quantified with a UV spectrophotometerat λ 280 nm. The characterization was conducted by Dot Blot test on a BCIP-NBT substrate, as well asSDS-PAGE and Western Blot with polyacrylamide gel concentrations of 7.5% and 17.5%. Validationwas performed using solutions containing glycerol and m-cresol as matrices spiked with rhINS. Thelinearity test in the rhINS concentration range of 80.11-200.28 μg/mL (r = 0.99) showed the linearresult. The accuracy and precision obtained an average of 99.11%±5.01 and 3.91%, while the LODand LOQ were 22.05 μg/mL and 73.51 μg/mL, respectively. Human insulin was stable at 2-8oC for 24hours (α: 0.05, ANOVA). In conclusion, in-house produced IgG polyclonal antibodies and goat anti-IgG peroxidase conjugate can be used for routine testing of human insulin.
Enhancing Kojic Acid Production in Aspergillus oryzae: Leveraging Crude Cellulase from Achatina fulica for Strain Improvement via Protoplasting and UV Mutagenesis Putri Mulia, Ocha; Larosa, Febri LS; Septiarini, Tri Hastuti; Rahmasari, Ratika; Suryadi, Herman
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.693-701

Abstract

This study aims to prove the ability of crude cellulase enzymes from snails for protoplasting Aspergillus oryzae cells and its application for strain improvement with UV mutagenesis. Snail enzyme was obtained from Achatina fulica by dissolving its digestion track and fractionating it with ammonium sulfate. The activity of fractions was measured Spectrophotometrically and used for cell protoplasting for 2 hours, then irradiated with UV for 10, 15, and 20 minutes, respectively, with 5 cm in the distance. Screening of mutants is carried out with 1% FeCl3, and the potential mutant strain was tested for kojic acid production in an aerobic state and determined by Spectrophotometry at 268 nm. The cellulase activity in crude snail enzyme was 11.5807 U/ml and increased to 16.3984 U/ml after fractionation. The best protoplast formation was obtained with a 60% fraction, which showed transparent performance under the microscope. The UV mutagenesis of protoplasts showed that the highest number of potential mutants was obtained from UV treatment for 15 minutes (41.67%). The potential mutants look dark brown (DBC), such as strain 10H3, and produced higher kojic acid concentration than the parent strain. In conclusion, UV mutagenesis of Aspergillus oryzae through protoplasting by crude cellulase of snail enzyme was effective and improved kojic acid concentration.
Antibacterial Synergy Detection of Lignin Extract from Oil Palm Empty Fruit Bunches (Opefb) Combined with Amoxicillin Against Staphylococcus Aureus Using The Azdast Method Rifki Anshory Hendri; Herman Suryadi; Hayun Hayun
Journal of Applied Agricultural Science and Technology Vol. 8 No. 1 (2024): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v8i1.191

Abstract

Oil palm empty fruit bunches (OPEFB) are waste generated by the palm oil industry and are generally considered an environmental problem due to their large quantity and difficult recyclability. This study's objective was to examine the potential of OPEFB lignin extract combined with amoxicillin in inhibiting the growth of Staphylococcus aureus bacteria. The Synergism Test of the OPEFB lignin extract-antibiotic combination was conducted using the AZDAST method (Ameri Ziaei Double Antibiotic Synergism Test). The lignin extraction process was a steam process using a 4% oxalic acid solution under optimal conditions. Then, Calcium ions precipitate 4% oxalate by adding Ca(OH)2 to the extract. The Lignin content was determined by UV-Vis spectrophotometry at 280 nm, and then the lignin extract was combined with antibiotics for antibacterial assay. The results showed a lignin content of 1.06 %. The AZDAST test results revealed clear zones from the combination of OPEFB lignin extract and amoxicillin with a diameter of 20.23 mm for the extract containing oxalate and 15.83 mm for the oxalate-free extract. Meanwhile, on average, the clear zone of single amoxicillin was only 14.13 mm. Based on these results, the combination of OPEFB lignin extract with amoxicillin was stronger than amoxicillin alone in inhibiting the growth of S. aureus, but it was not significantly different (p-value> 0.05). In conclusion, the potential of OPEFB lignin extract synergistically worked with the antibiotic amoxicillin in inhibiting the growth of S. aureus.
POLA MANCARAK DALAM PERMAINAN CENANG TIGO DI KAMPUNG AIA MARUOK KINALI, KABUPATEN PASAMAN BARAT Suryadi, Herman; Jaya, Susandra
EZRA SCIENCE BULLETIN Vol. 2 No. 2 (2024): July-December 2024
Publisher : Kirana Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58526/ezrasciencebulletin.v2i2.192

Abstract

Kesenian cenang tigo adalah tradisi masyarakat kampung Aia Maruok Kinali, Kabupaten Pasaman Barat. Cenang tigo biasanya dimainkan sebagai hiburan masyarat untuk mengisi waktu senggang. Umumnya permainan cenang tigo dimainkan oleh perempuan Minangkabau dari anak-anak samapai orang dewasa. Tidak hanya itu permainan tradisi cenang tigo juga dimainkan pada saat adanya acara pernikahan, dan manitia anak atau disebut dengan istilah babako. Penelitian dalam penulis kajian kali ini bersumber dari kesenian cenang tigo, yaitu apa pengaruh dari pola permainan mancarak dalam kesenian cenang tigo. Metode yang digunakan dalam penelitian pola permainan mancarak dalam kesenian cenang tigo adalah fungsi dari permainan pola mancarak. Manciek adalah permainan yang memiliki pola permainan dasar tetapi terdapat semacam pola paningkah yang disebut pola manigo (pola tiga). Manduo adalah permainan yang dimain sama seperti manciek tetapi tidak memakai pola minigo (pola tiga). Sedangkan mancarak adalah permainan pola peningkah antara permainan pola manciek dengan pola manduo yang mana dalam permainannya bersifat bebas tidak terikat seperti pola manciek dan manduo.
Expression and purification of recombinant human granulocyte colony‐stimulating factor (rG‐CSF) from Pichia pastoris Sembiring, Enny Rimita; Fuad, Asrul Muhammad; Suryadi, Herman
Indonesian Journal of Biotechnology Vol 29, No 4 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.93609

Abstract

Recent advances in biotechnology have sparked global interest in developing biosimilar drugs, particularly those containing physiologically active proteins, such as growth factors and cytokines. The methylotrophic yeast Pichia pastoris can produce and secrete fully active heterologous proteins with strong secretory capacity and low levels of native proteins and has the ability to achieve high cell densities. In this study, a yeast‐based system was used to express and purify recombinant human granulocyte colony‐stimulating factor (rG‐CSF). Cultures were induced every 12 h for 48 h to express rG‐CSF, and parameters such as cell density, media pH, and cell dry weight were observed. Cell density increased along with the corresponding secretion of rG‐CSF during the induction period, as determined by Western blot assay, while the pH of the media remained stable. Ammonium sulfate at different saturation levels was used to precipitate the recombinant protein, with the highest total protein content determined spectrophotometrically at 29.6 µg/mL. Ni‐NTA resin with affinity column purification was used to purify the recombinant protein. The purified protein showed rG‐CSF with a molecular weight of approximately 18 kDa based on SDS‐PAGE analysis and immuno slot blot assay detected in purple. Overall, the study results indicated that the production and purification of rG‐CSF was successful, although optimization was required. The long‐term goal of this research is to discover alternative methods and sources for producing biosimilars of the therapeutic protein rG‐CSF, which can be utilized in the pharmaceutical industry to support health programs, particularly cancer treatment.
Antibacterial effects of Andrographis paniculata extract, Curcuma domestica extract, chloramphenicol and their combinations on the growth of Salmonella typhi bacteria Rahmadi, Agus; Elya, Berna; Suryadi, Herman; Prasetio, Nisrina Putri; Kamaruzzaman, Mohd Amir
JURNAL ILMU KEFARMASIAN INDONESIA Vol 22 No 2 (2024): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v22i2.1677

Abstract

Typhoid fever caused by Salmonella typhi remains a serious health threat. Although standard treatment with antibiotics such as chloramphenicol has helped reduce mortality rates, bacterial resistance to this antibiotic is increasing. New treatment approaches are urgently needed, including combining antibiotics with natural compounds from medicinal plants, such as Andrographis paniculata and Curcuma domestica. This study aimed to compare the antibacterial effects of A. paniculata extract, C. domestica extract, chloramphenicol, and their combinations on the growth of S. typhi. This in vitro experimental study used the disc diffusion method to evaluate antibacterial activity. Antibacterial activity tests were performed against S. typhi using discs soaked in 70% ethanol extract solutions of A. paniculata and C. domestica, chloramphenicol, and their combinations. Inhibition zones were measured after incubation for 24 hours at 37°C. Chloramphenicol showed the strongest antibacterial activity with a mean inhibition zone of 28.33 ± 0.58 mm. Single extracts of A. paniculata and C. domestica had relatively weak antibacterial activity (inhibition zones of 9.67 ± 1.15 mm and 9.83 ± 0.29 mm) and there was no significant difference between them (p>0.05). Combinations of extracts with chloramphenicol showed increased antibacterial activity compared to single extracts (inhibition zones of 23.17 ± 1.26 mm for A. paniculata + chloramphenicol and 21.00 ± 2.65 mm for C. domestica + chloramphenicol) and there were significant differences between combinations and single extracts (p<0.05), but still lower than single chloramphenicol and statistically significant (p<0.05). Although combining medicinal plant extracts with chloramphenicol increased antibacterial activity compared to single extracts, it did not exceed single chloramphenicol.