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PENGENDALIAN HAMA ULAT KROP (CROCIDOLOMIA BINOTALIS ZELL.) PADA TANAMAN KUBIS (BRASSICA OLERACEA L.) DENGAN APLIKASI AGENSI HAYATI STREPTOMYCES SP. Salfi Mauludhiea Firli; Wiwin Windriyanti; Penta Suryaminarsih
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 7, No 1 (2022): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v7i1.6823

Abstract

Tanaman Kubis merupakan salah satu jenis memiliki nilai ekonomi yang cukup tinggi akan tetapi produksinya mengalami penurunan. Salah satu faktor penyebabnya ialah  serangan hama Ulat Krop (Crocidolomia binotalis). Pengendalian yang dapat dilakukan untuk mengurangi dampak dari serangan hama tersebut ialah dengan memanfaatkan agensi hayati Streptomyces sp.  Penelitian ini bertujuan untuk mengetahui pengaruh aplikasi Streptomyces sp. untuk mengendalikan Crocidolomia binotalis serta menghitung intensitas kerusakannya.Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan satu faktor yaitu aplikasi agensi hayati sebelum infestasi larva, setelah infestasi larva, dan tanaman yang diinfestasikan larva saja sebagai kontrol.Hasil penelitian didapatkan bahwa gejala yang timbul pada larva yaitu perubahan warna tubuh menjadi lebih pucat, tekstur tubuh lunak, dan kebasahan.Gejala diteruskan dengan warna tubuh larva berubah menjadi coklat kehitaman serta ukuran tubuh mulai mengkerut dan mengering. Aplikasi agensi hayati Streptomyces sp. pada tanaman kubis belum mampu untuk mengendalikan serangan hama ulat krop, ditunjukkan dengan presentase kematian yang sama antar perlakuan yaitu sebesar 1,39 %, akan tetapi agensi hayati Streptomyces sp. yang diaplikasikan sebelum infestasi larva mampu menurunkan intensitas kerusakan tanaman kubis menjadi 10,75% jika dibandingkan dengan Streptomyces sp. yang diaplikasikan setelah infestasi larva sebesar 13,82% dan kontrol yaitu sebesar 15,35%.
Potential of Multientomopa Streptomyces sp. and Tripchoderma sp. in Potato Extract Broth and Glucose Nitrate Broth Media on Pests (Spodoptera litura) Eating Behavior by in Vitro Test Ika Nur Fitriana; Penta Suryaminarsih; Tri Mujoko
Nusantara Science and Technology Proceedings 4th International Seminar of Research Month
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2019.0438

Abstract

Streptomyces sp. and Trichoderma sp. are biological agents that have high potential to produce secondary metabolites to reduce losses of agricultural products caused by pests. This study aims to determine the multientomopathogenic potential of Streptomyces sp. and Trichoderma sp on EKG and GN media on feeding behavior of Spodoptera litura pests in vitro. 10 S. litura (2nd instar) were placed in a box then the larvae with each entomopathogenic treatment. Besides, feed as much as 5 grams for each box every day for 7 days. Observations were made by weighing the difference in daily feed weight for 7 days. Effect of treatment on feeding activity of Spodoptera sp. on the 7th day after application showed that EKGST 5: 1 treatment had the highest value due to the activity of the chitinase enzyme from Streptomyces sp. and Trichoderma sp. Calculation of the ability of chitin hydrolysis by the enzyme chitinase is done by calculating the diameter of the clear zone that is formed then reduced by the diameter of the blotting paper containing supernatant secondary metabolites. Chitinase testing was carried out to determine the ability of secondary metabolites produced by Streptomyces sp. and Trichoderma sp. in hydrolyzing chitin. The widest zone is produced by multientomopathogenic Streptomyces sp. and Trichoderma sp. in EKGST 5: 1 treatment 3cm. Damage to the digestive tract due to the chitinase enzyme causes a decrease in the ability to absorb nutrients that have an impact on the reduced activity of Spodoptera litura.
The Effect of Temperature Pressure on Multiantagonists Streptomyces sp., Tricho-derma sp. Biological Control of Fusarium oxysporum Wilt Pathogens Penta Suryaminarsih; Dita Megasari; Tri Mujoko
Nusantara Science and Technology Proceedings Seminar Nasional Magister Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2020.0609

Abstract

Climate change due to global warming can increase soil temperatures. The increase in soil temperature can be influenced by air temperature which can affect the life of soil microorganisms. Streptomyces sp. and Trichoderma sp. is a soil microorganism that acts as a Biological Control Agent (APH) of plant pests and diseases. This study aims to determine the inhibitory ability of Streptomyces sp. and Trichoderma sp. at high temperature against Fusarium sp. causes of wilt disease in chilies. This research used factorial completely randomized design. The first factor is room temperature 29-31 ° C, temperature 50 ° C, and temperature 70 ° C. The second factor is the type of andosol soil (UPN land) and regusol (Pare village land). Observations were made on the number of colonies of Streptomyces sp. and Trichoderma sp. as well as inhibition against the fungus Fusarium sp. The results showed that the multiantagonist inhibition of Streptomyces sp. and Trichoderma sp. against F. ox-ysporum at 70 ° C in 90% andosol soil and the lowest at 70 ° C on 31% regusol soil.
Prospects of Biological Control of Green Ladybugs (Nezara viridula) Using Streptomyces spp. Laksamana Agadhia Raharjo; Penta Suryaminarsih; Dita Megasari
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1504

Abstract

Ecosystem damage due to the use of chemical pesticides increases every year, therefore alternative searches need to be known and developed. APH (Biological Control Agency) is a beneficial microbe that can control pest and disease populations. Streptomyces spp. Is a gram-positive soil bacterium that can be used against green ladybugs because Streptomyces spp. Able to produce chitinase enzymes that can break down chitin compounds in Exo – Skeleton Arthropod animals The purpose of this study was to determine the prospects for the use of Streptomyces spp. as APH of green shieldbugs.The description of this article includes: research results of Streptomyces spp., morphology, bio-ecology and bio-molecular green shieldbugs (Nezara viridula), as well as prospects for the use of Streptomyces spp. the results show that the use of Streptomyces spp. is able to control green ladybugs, because the chitinase enzyme produced can break down the chitin compound.The development of Streptomyces spp. has a lot of space, developments in the production of chitinase enzymes can be implemented and have clear potential.
Prospect of Utilization of Microorganisms Streptomyces sp. and Trichoderma sp. in Supporting Sustainable Agriculture in the Age of Modern Agriculture Arga Dwi Indrawan; Penta Suryaminarsih; Tri Mujoko
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1506

Abstract

The geographical condition of Indonesia's agriculture in the tropics supports agricultural business throughout the year through agricultural intensification. Agricultural intensification brings benefits to agricultural productivity, but also causes farmers to be dependent on pesticides and inorganic fertilizers in excess, causing long-term degradation of agricultural land productivity. Susstainable Agriculture is an approach to increase agricultural productivity while still being friendly to the agricultural ecosystem. Sustainable agriculture can be achieved through the preservation of agricultural resources, one of which is the use of beneficial microorganisms. Stre ptomyces sp. and Trichoderma sp. is a multi-role microorganism as a supporter of plant growth, biological agent in pest control, and helps increase soil fertility. The use of these microorganisms has good prospects for sustainable modern agriculture in order to achieve food security but also has obstacles that need to be overcome through further research.
Utilization of Fobio and Streptomyces sp. in Improving the Growth of Shallots Sri Wiyatiningsih; Penta Suryaminarsih; Gusdino Hasyidan
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1507

Abstract

Shallots (Allium ascalonicum L.) is a spice vegetable commodity that is needed by the community as a cooking spice to increase the taste of eating pleasure and for traditional medicines that are efficacious for health. Control using chemicals is believed to be effective in overcoming pathogen attacks, but the continuous addition of chemical pesticides will have a negative impact on the environment and consumers. To deal with the negative impact of the use of these chemicals, namely by applying a sustainable farming system that was originally a conventional farming system and includes organic agriculture (Fobio biopesticide application) and biological (Application of Streptomyces sp. biological agents). The purpose of this study was to determine the effectiveness of the formulation of the biopesticide Fobio and the biological agent Streptomyces sp. to increase the growth of leeks and minimize the negative impact of using fungicides in Bulugunung Village, Plaosan District, Magetan Regency but still have the potential for maximum harvest success. The study was conducted from March to May 2021. This study used a Completely Randomized Design (CRD) consisting of 4 treatments with 6 replications. Each experimental unit consisted of 100 plants. Observation parameters consisted of leaf height and number of leaves of shallot plants. The results showed that the application of biopesticide Fobio and biological agents Streptomyces sp. did not significantly affect the height of the shallot plant and had a significant effect on the number of leaves of the shallot plant
Exploration and Identification of the Entomopathogenic Fungus Metarhizium anisopliae in Corn Plants in Sebandung Village, Sukorejo, Pasuruan Rateh Lailatul Risdiyanti; Wiludjeng Widayati; Penta Suryaminarsih
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur 2021
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2002

Abstract

Metarhizium anisopliae is a biological control agent (BCA) that have been developed to control plant pest in this disruption era. These fungi penetrate the insect body through its skin between the head and the thoraxs also in between the joint. The purpose of this research is to identify the characteristic of the fungi M. Anisopliae from the exploration in sebandung village, sub-district Sukorejo, district Pasuruan. Data analysis is done by using descriptive data. Step of the research involves (1) sample gathering from the soil in the cornfield that has been predicted to have BCA biodiversity, after that determined 5 sampling points diagonally, each sample contains 100 gr of soil from the depth of 10 -20 cm, (2) fungi baiting by using a mealworm (Tenebrio Molitor) as a bait, (3) M. Anisopliae isolate using PDA (Potato Dextrose Agar), (4) identification is conducted by macroscopic and microscopic. The identification result shows that the isolate has a white mycelium that forms into a mossy green spore, fine texture, easily spread, and insulated mycelium, straight conidiophore, hyalin, and branching, single-cell conidia, hyalin, round cylinder type, also white to the mossy green colony. Parasitism test results show that T.molitor larvae develop a hard body, have a murky color, covered in white hyphea till it transforms into moosy green spore with a fine texture and easily spread, the spore is the final form of the fungi propagule existence in the host as a survival phase in extreme condition.
The Presence of Soybean Plant Insects (Glycines Max L. Merril) in the Vegetative Phase with the Application Treatment of Streptomyces sp. and Trichoderma sp. Nugraha Iqbal Avrianto; Penta Suryaminarsih; Wiludjeng Widajati
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur 2021
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2005

Abstract

Soybean (Glycine max (L.) Merill) is one of the main food crops in the community. In East Java, productivity has declined due to various factors, including pest attacks. This study aims to determine the diversity of insects by administering a mixture of biological control agents (BSAs) between Streptomyces sp. and Trichoderma sp. as an entomopathogen applied to soybean plants in the vegetative phase. The soybean plants were planted in plastic bags on the ground of the Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran Jawa Timur. The aim of this study was to determine the effect of Streptomyces sp. and Trichoderma sp. the type and population of parasites. This study used a randomized two-way block design of 13 treatments repeated 3 times. Based on the observations, the total number of insects found in the experimental field of UPN veterans Jawa Timur was 2,360 individuals, made up of six orders of insects. The four orders are divided into 8 families and 8 species. Hemiptera species became the most dominant order (2202 individuals; 93%), Orthoptera (108 individuals; 5%), Lepidoptera (18 individuals; 1%) and Diptera (32 individuals; 1%) . The use of Streptomyces sp. and Trichoderma sp. the single comparison treatment is more effective than the combined comparison on the pest population. The main insects include Aphid spp, Bemisia tabaci and Nezera viridula.
Pest and Disease Control in Cavendish Banana Seedlings Resulting from Tissue Culture Indah Sari Dwi Agustin; Penta Suryaminarsih; Putranto Sasikirono; Yenny Wuryandari
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur 2021
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2013

Abstract

Cavendis banana cultivation can use tissue culture as one of the developments of cultivation in the era of disruption. However, the results of tissue culture are very susceptible to attack by nuisance organisms during acclimatization to plant culture. The control carried out against the attack of plant-disturbing organisms in addition to using fungicides and insecticides, also applies preventive control. The purpose of this study was to determine an effective and smart way to control pests and diseases in tissue cultured Cavendish banana seedlings. Preventive control of pests and diseases using the method of thinning the seeds and soaking the seeds with fungicides. Data analysis was carried out using descriptive and parametric data. This study was conducted using a completely randomized design (CRD) with four control treatments. Each treatment was repeated 4 times. The control treatments carried out consist of: Control (A), preventive control (B), chemical control (C), and a combination of preventive and chemical control (D). The results of the control carried out showed that the combination of preventive and chemical control treatments gave significant results in inhibiting the attack of pests and diseases of Cavendish banana seedlings from tissue culture.
KEANEKARAGAMAN SERANGGA HAMA PADA TANAMAN PADI DENGAN APLIKASI Streptomyces sp. DAN Trichoderma sp. DI DESA MOJOTENGAH KABUPATEN GRESIK Alifta Wiranthi; Penta Suryaminarsih; Wiwin Windriyanti
Plumula : Berkala Ilmiah Agroteknologi Vol 9 No 2 (2021): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v9i2.59

Abstract

Rice plants are often disturbed by plant-disturbing organisms (OPT) which results in decreased quality and quantity of rice production. efforts to increase the production of materials, in order to obtain production results with high quality and strength is by means of integrated pest control (IPM). The problem of pests and plant diseases is an inseparable part of plant cultivation. The use of biological agents is very cost effective and reduces the negative effects caused by the use of chemical pesticides. Microorganisms are biological agents capable of controlling insect pests. Genus Steptomyces sp. and Trichoderma sp. produce the enzyme chitinase. This study aims to determine the effect of the biological agency application Streptomyces sp. and Trichoderma sp. on the type, composition, and population of insects, the index of pest diversity on rice fields in Mojotengah Village, Gresik Regency. This study used a survey method using direct observation, insect capture was carried out using a sweep net, a yellow sticky trap, and a pitfall trap. The results of research on rice fields with insect populations found on land with biological agency applications were 280 individuals and 775 individuals without biological agency. Application of biological agents to Streptomyces sp. and Trichoderma sp. affect the number of pest populations. Application of biological agents to Streptomyces sp. and Trichoderma sp. effect on the index value of species diversity including the medium category, the evenness index on the two lands is in the medium category, the species richness index shows the high species richness level in both lands, the dominance index shows the value is included in the medium category.