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UJI EFEKTIVITAS MINYAK ATSIRI SERAI WANGI (Cymbopogon nardus L.) DALAM MENGENDALIKAN JAMUR (Alternaria porri) PENYEBAB PENYAKIT BERCAK UNGU PADA TANAMAN BAWANG PREI (Allium Fistulosum L.) Abifah, Serlia Nur; Prasetyawati, Endang Triwahyu; Suryaminarsih, Penta
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.8781

Abstract

The growth and production of oil palm is greatly influenced by the presence of Plant Pest Organisms (OPT), one of which is purple spot disease caused by the fungus Alternia porri. Control efforts using vegetable fungicides, namely citronella essential oil. The aim of this research is to determine the concentration of citronella essential oil as a vegetable fungicide that is effective in suppressing the growth of the Alternaria porri fungus in vitro. This research was carried out from October-December 2023. The location of this research was carried out at the Plant Health Laboratory, Faculty of Agriculture, National Development University "Veteran" East Java. The research design used was a Completely Randomized Design (CRD) using a single factor of citronella essential oil concentration, namely 0%, 10%, 20%, 30% and 40%. Observation variables include the inhibition test. The data from the research was carried out using Analysis of Varience (ANOVA) and continued with a multiple comparison test using the BNJ advanced test at a real level of 5% using SPPS software version 21. The results of the research showed that the largest percentage of inhibitory power on the last day of observation was obtained at a concentration of 40%, namely amounting to 32.92% and 30% concentration, namely 20.29%, while the lowest inhibitory power was obtained from the 20% concentration treatment, namely 7.74% and 10% concentration, namely 8.89%. The higher the concentration of citronella essential oil used, the stronger the inhibition of fungal growth will be.Keywords : essential oils, citronella, concentrate, Alternia porri
Bio-Priming with Trichoderma spp. to Suppress Aspergillus flavus, the Causal Agent of Damping-off Disease in Peanuts Anita, Tri Yuli; Suryaminarsih, Penta; Rahmadhini, Noni
BIOEDUSCIENCE Vol 9 No 2 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/14937

Abstract

Background: Aspergillus flavus is a fungus that produces aflatoxin, a toxic compound that can contaminate food products, especially nuts. The impact caused by A. flavus causes significant losses for peanut-producing countries in cases of international trade. A. flavus infection in peanuts causes physical changes and quality, as well as a decrease in seed germination. Environmentally friendly control efforts for A. flavus can be carried out by utilizing biological agents such as Trichoderma spp. This study aims to evaluate the ability of T. harzianum and T. asperellum to inhibit the growth of A. flavus. This study also evaluates bio-priming methods enriched with Trichoderma spp. to optimize peanut seed germination. Methods: The research used a factorial Completely Randomized Design (CRD) of 2 factors. The first factor is the isolate species Trichoderma spp. namely T. harzianum (T1), T. asperellum (T2), and Control (T0), namely A. flavus without biological control treatment. The second factor is the application method, which includes the bio-priming (B1) and bio-matrixpriming (B2) methods. Each treatment combination was repeated 4 times, and the number of seeds used was five in each unit. Results: T. harzianum showed the highest inhibitory ability against A. flavus in the in vitro test, namely 49%. Bio-priming treatment containing the active ingredient T. harzianum is the best treatment for increasing peanut germination by 95%. Conclusions: The research results showed that bio-priming treatment with the active ingredient T. harzianum increased the germination rate significantly compared to other treatments.
POTENSI METABOLIT SEKUNDER STREPTOMYCES SP. SEBAGAI BIOPESTISIDA PADA BERBAGAI KOSENTRASI TERHADAP PENYAKIT MOLER BAWANG MERAH Agustin, Indah Sari Dwi; Suryaminarsih, Penta; Wiyatiningsih, Sri
Agros Journal of Agriculture Science Vol 25, No 1 (2023): edisi JANUARI
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i1.2597

Abstract

Penyakit moler atau layu pada tanaman bawang merah disebabkan oleh jamur Fusarium sp. Penyakit ini tergolong penyakit penting pada tanaman bawang merah karena dapat menimbulkan kerusakan hingga 50%. Salah satu upaya untuk mengendalikan penyakit moler adalah dengan menggunakan metabolit sekunder Streptomyces sp yang berasal dari lahan bawang merah di Pare-Kediri. Tujuan dari penelitian ini adalah untuk mengetahui potensi metabolit sekunder streptomyces sp. yang mengandung antibiosis penghambat pertumbuhan Fusarium sp. pada berbagai kosentrasi terhadap penyakit moler bawang merah .Penelitian menggunakan Rancangan acak lengkap dengan faktor perlakuan konsentrasi metabolit sekunder 5%, 10%, 15% dan 20%. Masing–masing perlakuan diulang sebanyak 5 kali dan diberikan 5 unit tanaman pada masing–masing ulangan. Pengamatan dilakukan terhadap masa inkubasi, keparahan penyakit serta pertumbuhan vegetative. Parameter pengamatan dilakukan terhadap masa inkubasi, keparahan penyakit,tinggi tanaman dan jumlah daun. Aplikasi metabolit sekunder Streptomyces sp. konsentrasi 20% pada semua parameter pengamatan menunjukkan hasil terbaik dibandingkan dengan perlakuan yang lain.
EFIKASI Streptomyces sp. TERHADAP PENYAKIT LAYU FUSARIUM PADA TANAMAN CABAI MERAH (Capsicum annuum L.) DENGAN WAKTU APLIKASI YANG BERBEDA Ekayanti, Savira Aprilia; Suryaminarsih, Penta; Mujoko, Tri
Agros Journal of Agriculture Science Vol 25, No 4 (2023): edisi Oktober
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i4.3547

Abstract

Fusarium wilt disease in red pepper plants is caused by the pathogenic fungus Fusarium sp. This disease is classified as an important disease of red chili plants because it can cause damage up to 50%. One of the efforts to overcome fusarium wilt disease is to use biological agents Streptomyces sp. which comes from the field of shallots and tomatoes in Pare, Kediri. The purpose of this study was to determine the effect of the application of Streptomyces sp. Against Fusarium wilt disease in red pepper plants with a wide range of application times. This study used a Factorial Complete Randomized Design with SP and BMP isolate type treatment, as well as application time treatment before planting, at the same time planting and after planting. Each treatment was repeated 5 times and given 5 plant units on each repetition. Observation parameters were carried out on the incubation period, disease severity, plant height, number of leaves, flowering age and number of flowers. Application Streptomyces sp. SP isolate with application time before planting showed the best results compared to other treatments. Keywords: Application Time, Fusarium sp., Streptomyces sp.  INTISARI                                                 Penyakit layu fusarium pada tanaman cabai merah disebabkan oleh jamur patogen Fusarium sp. Penyakit ini tergolong ke dalam penyakit penting tanaman cabai merah karena dapat menyebabkan kerusakan hingga 50%. Salah satu Upaya dalam mengatasi penyakit layu fusarium adalah menggunakan agensia hayati Streptomyces sp. yang berasal dari lahan bawang merah dan tomat di Pare, Kediri. Tujuan dari penelitian ini adalah untuk mengetahui mengetahui pengaruh apikasi Streptomyces sp. terhadap penyakit layu fusarium pada tanaman cabai merah dengan berbagai macam waktu aplikasi. Penelitian ini menggunakan Rancangan Acak Lengkap Faktorial dengan perlakuan jenis isolate SP dan BMP, serta perlakuan waktu aplikasi sebelum tanam, bersamaan tanam dan setelah tanam. Masing-masing perlakuan diulang sebanyak 5 kali dan diberikan 5 unit tanaman pada masing-masing ulangan. Parameter pengamatan dilakukan terhadap masa inkubasi, keparahan penyakit, tinggi tanaman, jumlah daun, umur berbunga dan jumlah bunga. Aplikasi Streptomyces sp. isolat SP dengan waktu aplikasi sebelum tanam menunjukkan hasil terbaik dibandingkan dengan perlakuan yang lain. Kata kunci: Waktu Aplikasi, Fusarium sp., Streptomyces sp. 
POTENSI FORMULASI BIOFUNGISIDA Bacillus sp. (Bth-22) DAN Streptomyces sp. (TMP) TERHADAP PERKECAMBAHAN BENIH JAGUNG Maharani, Salsabilla Diva; Purnawati, Arika; Suryaminarsih, Penta
Agrika Vol. 19 No. 1 (2025): MEI 2025
Publisher : Badan Penerbitan Universitas Widyagama Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31328/ja.v19i1.6754

Abstract

Jagung (Zea mays) adalah salah satu tanaman pangan utama yang memiliki peran penting dalam ketahanan pangan global. Fusarium sp. merupakan patogen tanah yang sering ditemukan pada lahan jagung dan dapat mengganggu perkecambahan dan pertumbuhan tanaman jagung. Penggunaan bakteri Bacillus sp. dan Streptomyces sp. sebagai bahan aktif biofungisida dapat meningkatkan persentase perkecambahan benih dan pertumbuhan tanaman jagung yang terserang patogen Fusarium sp.. Penelitian ini bertujuan untuk mengetahui pengaruh jenis media dan potensi formulasi biofungisida dengan bahan aktif Bacillus sp. dan Streptomyces sp. dalam menunjang perkecambahan dan pertumbuhan tanaman jagung. Penelitian dilakukan dengan Rancangan Acak Lengkap (RAL) dengan dua faktor. Faktor pertama yakni media produksi bakteri: K: Air rebusan kedelai, E: Estrak Kentang Gula (EKG), dan A: Air kelapa. Faktor kedua adalah perbandingan formulasi Bacillus sp. dan Streptomyces sp. yang terdiri atas 4 taraf yaitu BS0 (0:0), BS1 (3:3), BS2 (2:4), dan BS3 (1:5). Hasil penelitian menunjukkan tidak terdapat interaksi antara kedua faktor perlakuan. Perlakuan BS2 merupakan perlakuan terbaik yang memberikan presentase perkecambahan sebesar 82%, hari berkecambah 3.64 HST, jumlah daun pada 35 HST sebanyak 5.7 helai dan 62.8 cm.
Study of Streptomyces spp. to control purple blotch disease caused by Alternaria porri in shallot plant Risdiyanti, Rateh Lailatul; Rahmadhini, Noni; Suryaminarsih, Penta; Mujoko, Tri
CROPSAVER Vol 6, No 1 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i1.43647

Abstract

Alternaria porri is a fungal pathogen that causes purple blotch on shallots, this fungus can cause crop yield loss of 3 – 57%. A. porri was obtained from the Sumber Brantas onion farm and then isolated by planting infected tissue. Streptomyces spp. is a filamentous bacterium that is abundant in the soil and can be used as a biological agent, decomposer and plant growth promote. Streptomyces spp. was obtained one isolate from shallot land location in Pare, Kediri, East Java (BMP: Bawang Merah Pare) and three isolates from Sidera, Palu, Central Sulawesi (BMS: Bawang Merah Sidera) (BMS1, BMS2, BMS3). The purpose of this study was to determine the efficacy of Streptomyces spp. to control A porri consist of in vitro and In vivo antagonist test. Antagonist test was carried out by dual culture method and the Streptomyces spp. which can inhibit the development of A porri would be used antagonist test   in vivo. The study was designed using a Factorial Complete Randomized Design with two factors, candidate isolates of biological control agents and concentrations of Streptomyces spp. be diluted into 5%, 10%, and 15%, it will applied on shallot plant Bauji variety. The results of the In vitro test shown the highest inhibition zone from BMP 17.75% while BMS1 13.75%, BMS2 8,75%, and 8.50%. in vivo test shown lowest disease severity value in BMP 15% concentration was 1.13% while diseases severity of BMS1 was 1.27%, and BMS2 was 1.80%. Therefore, Streptomyces spp. has potency as an alternative pesticide for sustainable agriculture.
Effect of Fobio on Intensity of Moler Disease (Fusarium oxysporum) on Various Shallot Cultivars Elvira, Nolla Dwi; Wiyatiningsih, Sri; Suryaminarsih, Penta
CROPSAVER Vol 6, No 2 (2023)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cropsaver.v6i2.45747

Abstract

Fusarium oxysporum is the pathogen that causes moler disease which can reduce the productivity of shallot plants. The use of cultivars greatly affects the production. Control method that is more environmentally friendly is currently being pursued towards organic farming. Fobio is a biopesticide containing plant root microorganisms as biological control agents. This study aims to determine the effect of Fobio biopesticide on Fusarium wilt (moler) disease incidence and production of three shallot cultivars. The experiment was conducted in the shallot planting area of Sukorejo Village, Rejoso District, Nganjuk Regency, East Java. This experiment was arranged in the split plot design with two factors. The main plot of the concentration of the biopesticide formula (Fobio) consisted of 3 levels namely control (chemical fungicide), 5 ml/liter, and 10 ml/liter, and the subplot of shallot cultivar consisted of 3 types namely Tajuk, Biru Lanchor, and Super Philip. The observation in this study were the incubation period, disease symptoms, disease intensity, and shallot bulb yield. The results showed that Fobio biopesticide at the concentration of 10 ml/liter caused the same disease suppression as chemical fungicide (control) in the three shallot cultivars with the disease incidence of 18.78%. Fobio biopesticide concentration of 10 ml/liter had also the same effect as chemical fungicide on the production of those shallot cultivars, with the average wet weight of 2.25 kg/treatment and dry weight of 1.49 kg/treatment.
Biopestisida (FOBIO) Sebagai Penghambat Perkembangan Penyakit Layu Fusarium Pada Tanaman Pisang Di Provinsi Bali: Biopesticide (FOBIO) as an Inhibitor of Fusarium Wilt Disease Development in Banana Plants in Bali Province Rosyiidah Sholihin, Alyaa Arwaa; Wiyatiningsih, Sri; Suryaminarsih, Penta
Biofarm : Jurnal Ilmiah Pertanian Vol. 21 No. 1 (2025): BIOFARM JURNAL ILMIAH PERTANIAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pisang (Musa paradisiaca L.) merupakan salah satu kebutuhan pokok bagi masyarakat Bali, terutama umat Hindu, yang mendorong pengembangan budidaya secara intensif di berbagai jenis lahan. Namun, pengembangan ini dihadapkan pada kendala penyakit layu fusarium yang disebabkan oleh Fusarium oxysporum f.sp. cubense. Penelitian ini mengevaluasi efektivitas biopestisida FOBIO dalam menekan intensitas serangan fusarium serta meningkatkan pertumbuhan tunas pisang. Penelitian dilakukan menggunakan Rancangan Acak Kelompok Faktorial (RAKF) dengan dua faktor: ketinggian tempat (650-950 mdpl dan 25-40 mdpl) serta konsentrasi biopestisida (kontrol dan FOBIO 7,5 ml/liter). Hasil penelitian menunjukkan bahwa aplikasi FOBIO 7,5 ml/liter mampu menurunkan persentase serangan dari kategori berat menjadi sedang sebesar 18,9%. Serangan tertinggi terjadi di dataran tinggi (650-950 mdpl) dengan intensitas 34,1% (kategori berat), di mana FOBIO tidak efektif dalam menurunkan intensitas serangan. Meski tidak signifikan, aplikasi FOBIO meningkatkan jumlah tunas baru sebesar 8,4 tunas di dataran tinggi.
The Potency of Streptomyces spp. from Shallot Land as Entomopathogen of Onion Caterpillar Pest Spodoptera exigua Pratista, Ariq Dies; Windriyanti, Wiwin; Suryaminarsih, Penta; Mujoko, Tri; Indrawan, Arga Dwi; Harsiyanti, Harsiyanti
International Journal on Food, Agriculture and Natural Resources Vol 6, No 4 (2025): IJ-FANRES
Publisher : Food, Agriculture and Natural Resources - NETWORKS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46676/ij-fanres.v6i4.569

Abstract

Spodoptera exigua is a major pest of shallots (Allium ascalonicum L.), capable of causing up to 100% yield loss if uncontrolled. Streptomyces sp. is a soil-dwelling Actinomycetes bacterium that can be used to control S. exigua. This study aimed to determine the potential of Streptomyces spp. isolates from shallot fields as an entomopathogen against S. exigua. The research employed a Factorial Complete Randomized Design (CRD). The first factor consisted of one Streptomyces spp. isolate obtained from the Pare-Kediri region (BMP) and three isolates from Sidera-Palu (BMS1, BMS2, and BMS3). The second factor was the concentration of the Streptomyces sp. suspension consist of 0%; 5%; 10%; and 15% with three replication per treatment (48 units total). Data on larval mortality and damage intensity were analyzed using RStudio via ANOVA and DMRT (α=5%). The BMP (15%) treatment demonstrated the highest efficacy, achieving 76.67% larval mortality while maintaining the lowest damage intensity of 0.20% on shallot plants. These results highlight BMP isolate at 15% concentration as the most promising biocontrol agent and support its further field evaluation as a sustainable alternative to chemical insecticides.
POTENSI JAMUR ENDOFIT SEBAGAI AGENSIA HAYATI JAMUR Colletrothichum sp. PENYEBAB PENYAKIT ANTRAKNOSA PADA TANAMAN CABAI RAWIT Ika Noviyanti, Rizky; Purnawati, Arika; Suryaminarsih, Penta
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 7, No 2 (2022): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v7i2.6021

Abstract

Tanaman cabai rawit (Capsicum frutencens L) merupakan salah satu komoditas utama tanaman hortikultura yang dibudidayakan secara komersial di daerah tropis yang bernilai ekonomi tinggi di Indonesia. Satu diantara penyakit penting pada cabai rawit adalah penyakit antraknosa. Antraknosa merupakan penyakit utama tanaman cabai yang disebabkan oleh jamur Colletotrichum sp. Penggunaan pestisida sintetik yang kurang bijaksana ternyata banyak merugikan manusia dan agroekosistem. Oleh karena itu, perlu dilakukan upaya pengendalian penyakit yang ramah lingkungan dengan penggunaan agensia hayati. Beberapa penelitian menunjukkan jamur endofit dapat digunakan untuk meningkatkan ketahanan inang dari serangan patogen serta dapat memacu pertumbuhan tanaman dengan menghasilkan metabolit sekunder dan enzim tertentu. Penelitian ini dilaksanakan dari bulan April hingga Juni 2021. Tempat pelaksanaan penelitian di Laboratorium Kesehatan Tanaman Fakultas Pertanian Universitas Pembangunan Nasional “Veteran” Jawa Timur untuk In Vitro. Sedangkan, untuk In Vivo dilaksanakan di Screenhouse Kebun Bibit Wonorejo Surabaya. Penelitian ini bertujuan untuk mengetahui potensi jamur endofit dari tanaman cabai rawit sehat yang mampu menghambat pertumbuhan jamur Colletotrichum sp. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) non faktorial. Berdasarkan pengamatan, jamur endofit asal tanaman cabai rawit berpotensi menekan pertumbuhan jamur Colletotrichum sp. penyebab penyakit antraknosa.
Co-Authors Abifah, Serlia Nur Agustin, Indah Sari Dwi Aisyah Lulu Hariyanto Alifta Wiranthi Anita, Tri Yuli Ardhia Pramesti Cahyani Arga Dwi Indrawan Arika Purnawati Chairunnisa Faza Nabillah Diah Budi Kusumawati Didik Utomo Pribadi Didin Julia Trisnawati Difa Eka Pranoto Dinda Rizka Rahayu Dita Megasari Dwi Lestari Ekayanti, Savira Aprilia Elly Syafriani Elvira, Nolla Dwi Erlangga Dwi Fachruddin Fachruddin, Erlangga Dwi Fadila Suryandika Ferdianto Eko Wicaksono Firdi Ihza Rochman Guniarti, Guniarti Gusdino Hasyidan Gusdino Hasyidan Harsiyanti, Harsiyanti Haryani, Wiwik Sri Hery Nirwanto Hery Nirwanto, Hery Ida Retno Moeljani Iis Purnamawati Ika Noviyanti, Rizky Ika Nur Fitriana Ika Nur Fitriana Indah Sari Dwi Agustin Indrawan, Arga Dwi Jati Laksono, Roy Juniawan Laksamana Agadhia Raharjo Lestari, Safira Rizka Maemunah Maemunah Maharani, Salsabilla Diva Mahfud, Nur Aini Maroeto Maroeto Moch Sodiq MOCH SODIQ, MOCH Nawaal, Najvania Nolla Dwi Elvira Noni Rahmadhini Nugraha Iqbal Avrianto Nur Syiami, Aning Pangesti Nugrahani Prasetyawati, Endang Triwahyu Prasetyawati, Endang Triwahyu Pratista, Ariq Dies Putranto Sasikirono Ramdan Hidayat Raniah, Nabilla Nur Rateh Lailatul Risdiyanti Risdiyanti, Rateh Lailatul Rizky Ika Noviyanti Rosyiidah Sholihin, Alyaa Arwaa Sa'adah, Fatimah Lailatus Safira Rizka Lestari Salfi Mauludhiea Firli Serlia Nur Abifah Setyo Budi Santoso Siti Fatimatus Syahrok Siti Fatimatus Syahrok Sri Wiyatiningsih Sri Wiyatiningsih Sri Wiyatiningsih Syahrok, Siti Fatimatus Tri MUJOKO Ummah, Robithotul Wanti Mindari Wiludjeng Widajati Wiludjeng Widayati Wiludjeng Widiyati Windriyanti, Wiwin Wiwik Sri Harijani Wiwik Sri Harijani Yenny WURYANDARI