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Faktor Efektivitas Penggunaan Nematoda Entomopatogen Syahrok, Siti Fatimatus; Suryaminarsih, Penta
Exact Papers in Compilation (EPiC) Vol. 5 No. 1 (2023): Februari 2023
Publisher : Lembaga Penelitian Pengabdian Masyarakat Universitas KH. A. Wahab Hasbullah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32764/epic.v5i1.895

Pengendalian secara hayati menggunakan nematoda entomopatogen merupakan salah satu upaya dalam pengendalian hama ramah lingkungan serta mampu menjaga ekosistem lingkungan tanaman. Mekanisme patogenesitas NEP melalui bakteri yang berasosiasi dalam fase juvenil infektif sehingga mampu membunuh serangga hama. Keberadaan nematoda entomopatogen dipengaruhi oleh faktor biotik dan abiotik. Faktor biotik meliputi pemilihan jenis spesies, jumlah spesies dan fase hidup. Sedangkan factor abiotik meliputi kondisi lingkungan, kelembaban tanah, suhu tanah, pH tanah, dan kandungan pupuk dan pestisida kimia dalam tanah. Perbanyakan secara konvensional yang efisien tenaga, waktu dan biaya yaitu secara in vivo dengan teknik baiting menggunakan larva Tenebrio molitor. Teknik aplikasi NEP dapat dilakukan dengan berbagai cara penyemprotan dan irigasi, baik menggunakan irigasi tetes, penyemprotan tekanan, penyemprotan kabut dan elektrostatis. Faktor yang perlu diperhatikan mengenai tekanan, jenis nosel dan hama sasaran. Salah satu faktor efektifitas NEP adalah pengembangan formulasi dosis yang efektif. Dosis terbaik pada konsentrasi Heterorhabditis sp. 400 JI/ml tercapai setelah 37,96 jam setelah aplikasi. Pengaplikasian Heterorhabditis sp. pada larva Spodoptera litura menimbulkan mortalitas sebesar 43% setelah 24 jam aplikasi. Pengembangan formulasi dosis harus terus dilakukan dikarenakan setiap spesies NEP memiliki kemampuan patogenesitas berbeda dan tergantung pada hama sasaran.

Potential of Streptomyces spp. From Peanut Plant Soil as an Entomopathogen of Pests Spodoptera Litura Ferdianto Eko Wicaksono; Wiwin Windriyanti; Penta Suryaminarsih
Demeter: Journal of Farming and Agriculture Vol. 2 No. 1 (2024): March 2024
Publisher : CV. Media Digital Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58905/demeter.v2i1.290

Peanuts are agricultural commodities that have high economic value and play an important role in meeting human needs, namely as a source of vegetable protein, oil and other nutrients, but domestic peanut production has not been able to meet the needs of the Indonesian people. The national demand for peanuts reaches 856.1 thousand tons per year, and the average consumption of peeled peanuts is 0.32 kg per capita every year. [1] In 2013 peanut production was 701,680 tons, then there was a decrease in production in 2014 to 638,896 tons, and continued until 2015 to 605,449 tons, thus showing a 13.7% decrease that occurred from 2013 to 2015. Low productivity is caused by various factors, one of which is the attack of pests and plant diseases. This study aims to determine the type of Streptomyces spp. Microbes with high chitinolytic content that can control armyworm pests (S.litura) on peanut plants. and influence of biological control agency Streptomyces spp as an entomopathogen of insect pests S. litura. This study was able to improve understanding of the influence of the biological control agency Streptomyces spp as an entomopathogen of S. litura insect pests to protect plants in the future. The results of the exploration of Streptomyces sp. acquired Colonies are irregular and branched coccus-shaped. The colony is tightly attached to the GNA medium, wavy white with a notched surface and a starchy texture. In addition, it has the largest chitinase enzyme content with a chitin zone diameter of 3 cm. PCR test Based on BLAST® result tabulation data that isolates belong to the genus Streptomyces sp. with an identity percentage of 99.86% with Streptomyces sp. VEL gene 17 to 16 rRNA, partial sequence with access code AB914463.2 The highest mortality was in the treatment (S0 RP K3) and (S1 RP K3) there was 60% this showed that with the administration of Streptomyces sp. With a concentration of 75% can cause very high mortality with abdominal contact poison in S. Litura pests

Potensi Streptomyces sp. Terhadap Penurunan Virulensi Bakteri Ralstonia solanacearum dalam Skala In vitro Sa'adah, Fatimah Lailatus; Suryaminarsih, Penta; Prasetyawati, Endang Triwahyu
Jurnal Agroekoteknologi Vol 16, No 1 (2024)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v16i1.23238

The demand for chili peppers in Indonesia continues to increase, but in 2021 there was a decrease in chili production. One of the main causes is pathogen attack, especially Ralstonia solanacearum which causes bacterial wilt disease in chili plants. This study aims to evaluate the potential of Streptomyces sp. isolates BMP and TMP to reduce the virulence of R. solanacearum. The research was conducted through a series of laboratory experiments, including an inhibition test with double-layer method and virulence reduction test on Triphenyl Tetrazolium Chloride (TZC) media. The results showed that the TMP isolate was able to produce an inhibition zone of 1.5 cm2 while the BMP isolate showed no inhibition zone. In addition, this study also evaluated the virulence reduction of R. solanacearum due to the treatment of Streptomyces spp. The virulence reduction test showed that TMP isolates were able to reduce virulence by 70% while BMP isolates were able to reduce the virulence of R. solanacearum by 4%. These findings provide new insights into the development of R. solanacearum control strategies. In conclusion, isolates of Streptomyces sp. especially BMP showed potential in reducing the virulence of R. solanacearum, thus providing new hope in efforts to increase chili production sustainably.

Application of Streptomyces sp. and Trichoderma sp. for Promoting Generative Plants Growth of Cherry Tomato (Lycopersicum cerasiformae Mill.) Nawaal, Najvania; Guniarti, Guniarti; Moeljani, Ida Retno; Suryaminarsih, Penta
PLANTA TROPIKA Vol 10, No 2 (2022)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v10i2.11706

Production of cherry tomatoes in Indonesia is still low, which might be due to the inappropriate planting and maintenance processes. This research applied biological agent microorganisms Streptomyces sp. and Trichoderma sp. as Plant Growth Promoting Microorganisms (PGPM) in sustainable agricultural systems. This study aimed to determine the effect of the concentration of microorganisms Streptomyces sp. and Trichoderma sp. on the growth and production of cherry tomato plants on the polybag scale. The study was arranged with different concentrations of microorganisms Streptomyces sp. and Trichoderma sp. These concentration applied consisted of 1 : 0 ; 0 : 1 ; 2: 2 ; 3 ; 1 and without PGPM, each repeated four times. The results showed that the treatment of PGPM Streptomyces sp. and without Trichoderma sp. (1:0) resulted in the shortest flowering period (33.99 days after planting). Meanwhile, the treatment without Streptomyces sp. and Trichoderma sp. (0 : 1) produced the highest solid weight fruit (69.82 grams/plant).

Carrier Materials Differences on Bioencapsulation Formulation Affect Bacillus sp. Isolates Wuryandari, Yenny; Suryaminarsih, Penta; Lestari, Safira Rizka
Nusantara Science and Technology Proceedings 8th International Seminar of Research Month 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4162

Biological agent Bacillus sp. Based on the results of previous research, direct use of isolated microorganisms BCZ14, BCZ16, and BCZ20 was able to suppress Fusarium wilt disease. However, Bacillus sp. formulations in suspension form are less effective when applied in the field. This research focuses on analyzing the viability of three isolates of Bacillus sp. against several combinations of bioencapsulation coating materials. The experimental design used was a completely randomized design with a combination of treatment of three isolates and three types of coating materials, namely sodium alginate (B1), carrageenan (B2), and chitosan (B3) each 2%. Each treatment combination was repeated five times. Based on the experiments that have been carried out, the bioencapsulation formulation using 2% chitosan coating material was able to maintain the viability of the three isolates of Bacillus sp. BCZ14, BCZ16, and BCZ20. The population density of the three isolates of Bacillus sp. persisted consistently from the first week to the last week of observation. Therefore, the 2% chitosan coating material can be said to be able to maintain the viability of Bacillus sp. within six weeks.

Carrier Materials Differences on Bioencapsulation Formulation Affect Bacillus sp. Isolates Yenny Wuryandari; Penta Suryaminarsih; Safira Rizka Lestari
Nusantara Science and Technology Proceedings 8th International Seminar of Research Month 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4162

Biological agent Bacillus sp. Based on the results of previous research, direct use of isolated microorganisms BCZ14, BCZ16, and BCZ20 was able to suppress Fusarium wilt disease. However, Bacillus sp. formulations in suspension form are less effective when applied in the field. This research focuses on analyzing the viability of three isolates of Bacillus sp. against several combinations of bioencapsulation coating materials. The experimental design used was a completely randomized design with a combination of treatment of three isolates and three types of coating materials, namely sodium alginate (B1), carrageenan (B2), and chitosan (B3) each 2%. Each treatment combination was repeated five times. Based on the experiments that have been carried out, the bioencapsulation formulation using 2% chitosan coating material was able to maintain the viability of the three isolates of Bacillus sp. BCZ14, BCZ16, and BCZ20. The population density of the three isolates of Bacillus sp. persisted consistently from the first week to the last week of observation. Therefore, the 2% chitosan coating material can be said to be able to maintain the viability of Bacillus sp. within six weeks.

Efikasi Formulasi Granular Kombinasi Trichoderma sp. dan Ekstrak Daun Bandotan terhadap Sclerotium rolfsii pada Porang (Amorphophallus oncophyllus) Raniah, Nabilla Nur; Nirwanto, Hery; Suryaminarsih, Penta
BIOEDUSCIENCE Vol 8 No 3 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/14880

Background: Amorphophallus oncophyllus is a tuber plant that is beneficial for various industrial sectors, but in its cultivation, it is often affected by the pathogen Sclerotium rolfsii, requiring control measures. Control efforts can be carried out using a combination of the biological agent Trichoderma sp. and the plant pesticide made from bandotan leaf extract. The combined control application can be formulated in a solid granular form. Method: This research was conducted both in vitro and in vivo. In vitro, a Completely Randomized Design (RAL) was used with the combination of Trichoderma sp. and bandotan leaf extract, divided into four levels: 0% (TB0), 5% (TB5), 10% (TB10), and 15% (TB15). In vivo, a 2-factor RAL was applied. The first factor was the concentration of the rice flour carrier material in four levels: 0% (B1), 25% (B2), 50% (B3), and 75% (B4). The second factor was the storage duration of the granular formulation, consisting of 0 weeks (M1), 3 weeks (M2), and 5 weeks (M3). Result: The study's results showed that the granular combination of Trichoderma sp. and 10% bandotan leaf extract reduced S. rolfsii infection by 64.25% and stimulated the growth of porang plant height. Conclusion: The treatment with the granular combination of Trichoderma sp. and 10% bandotan leaf extract, adding 25% rice flour and a storage period of 0-3 weeks, showed the best effect on the porang plant seedlings.

Application of Streptomyces sp. and Trichoderma sp. for Promoting Generative Plants Growth of Cherry Tomato (Lycopersicum cerasiformae Mill.) Nawaal, Najvania; Guniarti, Guniarti; Moeljani, Ida Retno; Suryaminarsih, Penta
PLANTA TROPIKA Vol. 10 No. 2 (2022)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v10i2.11706

Production of cherry tomatoes in Indonesia is still low, which might be due to the inappropriate planting and maintenance processes. This research applied biological agent microorganisms Streptomyces sp. and Trichoderma sp. as Plant Growth Promoting Microorganisms (PGPM) in sustainable agricultural systems. This study aimed to determine the effect of the concentration of microorganisms Streptomyces sp. and Trichoderma sp. on the growth and production of cherry tomato plants on the polybag scale. The study was arranged with different concentrations of microorganisms Streptomyces sp. and Trichoderma sp. These concentration applied consisted of 1 : 0 ; 0 : 1 ; 2: 2 ; 3 ; 1 and without PGPM, each repeated four times. The results showed that the treatment of PGPM Streptomyces sp. and without Trichoderma sp. (1:0) resulted in the shortest flowering period (33.99 days after planting). Meanwhile, the treatment without Streptomyces sp. and Trichoderma sp. (0 : 1) produced the highest solid weight fruit (69.82 grams/plant).

Potensi Paenibacillus polymyxa pada Media Molase terhadap Penyakit Layu Akibat Fusarium sp. pada Tanaman Cabai Rawit (Capsicum frutescens L.) Nur Syiami, Aning; Suryaminarsih, Penta; Mujoko, Tri
Plumula : Berkala Ilmiah Agroteknologi Vol. 13 No. 1: Januari 2025
Publisher : Program Studi Agroteknologi, Fakultas Pertanian, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33005/plumula.v13i1.221

Tanaman cabai rawit (Capsicum frutescens L.) adalah salah satu tanaman yang bernilai ekonomis tinggi. Produktivitas tanaman cabai rawit seringkali terhambat oleh penyakit layu Fusarium sehingga memerlukan upaya pengendalian. Paenibacillus polymyxa merupakan bakteri antagonis yang dapat mengendalikan patogen penyebab Layu Fusarium (Fusarium sp.). Penelitian bertujuan untuk mengetahui potensi P. polymyxa pada media pembawa molase terhadap penyakit layu (Fusarium sp.) tanaman cabai rawit dan mengetahui konsentrasi media pembawa molase yang efektif bagi P. polymyxa dalam mengendalikan penyakit layu (Fusarium sp.). Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan menggunakan 4 taraf perlakuan dan menggunakan 4 ulangan. Data dianalisis menggunakan analisis sidik ragam dan uji lanjut Beda Nyata Terkecil (BNT) pada taraf 5%. Hasil uji percobaan dilakukan uji lanjutan dengan BNJ 5%. Hasil penelitian menunjukkan bahwa penggunaan P. polymyxa + molase 30% efektif dalam menghambat dan menekan infeksi Fusarium sp. pada tanaman cabai rawit.

Effectiveness Soaking Duration of Rice Seed with Trichoderma sp. for Controlling Seed-Borne Pathogenic Fungi Mahfud, Nur Aini; Purnawati, Arika; Suryaminarsih, Penta
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.6847

Low rice productivity due to the use of low-grade seeds. The quality of the seeds can suffer a decrease caused by the attack of pathogenic fungi carried by the seeds both at the time of planting and storage, which will have an impact on the disruption of plant growth. Biopriming using Trichoderma sp. be an effort to control because it is antagonistic to pathogens. The success of biopriming is also influenced by the duration of seed priming. This study aims to examine the duration of soaking rice seeds with Trichoderma sp. the most effective for lowering seed-borne pathogenic infections. The study was conducted by providing seed soaking treatment using Trichoderma sp. suspension for 6 hours, 12 hours, and 24 hours with PDA medium incubation test method and Growing on Test. The parameters tested are the percentage of infection rate and the percentage of germination. Identification results showed that pathogenic fungi carried by seeds included Rhizopus sp., Fusarium sp., Aspergillus sp., and Mucor sp. The results showed the duration of soaking rice seeds with Trichoderma sp. more effective in decreasing the infection rate is at a 24-hour duration. However, the duration of soaking rice seeds with Trichoderma sp. there was no noticeable difference for the percentage parameter of seed germination.

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