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Pengaruh Formula Biopestisida (Fobio) sebagai PGPR terhadap Pertumbuhan Berbagai Kultivar Bawang Merah Nolla Dwi Elvira; Sri Wiyatiningsih; Penta Suryaminarsih
Biofarm Jurnal Ilmiah Pertanian Vol 19, No 2 (2023): BIOFARM JURNAL ILMIAH PERTANIAN
Publisher : Universitas Pekalongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31941/biofarm.v19i2.3079

Abstract

Bawang merah memiliki nilai ekonomi yang cukup tinggi. Penggunaan kultivar sangat berpengaruh terhadap peningkatan produksi. Formula biopestisida Fobio memiliki kemampuan untuk meningkatkan pertumbuhan tanaman karena adanya peran dari mikroorganisme sebagai Plant Growth Promoting Rhizomicroorganism (PGPR). Tujuan penelitian ini yaitu untuk mengetahui pengaruh biopestisida Fobio dalam meningkatkan pertumbuhan berbagai kultivar bawang merah. Penelitian ini dilaksanakan di lahan Desa Sukorejo, Kecamatan Rejoso, Kabupaten Nganjuk, Jawa Timur. percobaan ini menggunakan rancangan petak terbagi dengan dua faktor, petak utama konsentrasi formula biopestisida (fobio) 3 taraf yaitu kontrol, 5 ml/liter, 10 ml/liter dan anak petak kultivar bawang merah 3 jenis yaitu Tajuk, Biru Lanchor, Super Philip. Parameter pengamatan pada penelitian ini yaitu tinggi tanaman dan jumlah daun. Hasil penelitian menunjukkan Biopestisida fobio konsentrasi 10 ml/liter mampu meningkatkan pertumbuhan masing-masing kultivar. Nilai rata-rata tertinggi parameter tinggi tanaman terletak pada faktor perlakuan konsentrasi biopestisida 10 ml/liter yaitu 34,24 cm dan faktor perlakuan kultivar super philip yaitu 32,02 cm. Nilai rata-rata tertinggi jumlah daun terletak pada perlakuan kombinasi K10BT yaitu 31,67 helai.
EFIKASI Streptomyces sp. TERHADAP PENYAKIT LAYU FUSARIUM PADA TANAMAN CABAI MERAH (Capsicum annuum L.) DENGAN WAKTU APLIKASI YANG BERBEDA Savira Aprilia Ekayanti; Penta Suryaminarsih; Tri Mujoko
Agros Journal of Agriculture Science Vol 25, No 4 (2023): edisi Oktober
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v25i4.3547

Abstract

Fusarium wilt disease in red pepper plants is caused by the pathogenic fungus Fusarium sp. This disease is classified as an important disease of red chili plants because it can cause damage up to 50%. One of the efforts to overcome fusarium wilt disease is to use biological agents Streptomyces sp. which comes from the field of shallots and tomatoes in Pare, Kediri. The purpose of this study was to determine the effect of the application of Streptomyces sp. Against Fusarium wilt disease in red pepper plants with a wide range of application times. This study used a Factorial Complete Randomized Design with SP and BMP isolate type treatment, as well as application time treatment before planting, at the same time planting and after planting. Each treatment was repeated 5 times and given 5 plant units on each repetition. Observation parameters were carried out on the incubation period, disease severity, plant height, number of leaves, flowering age and number of flowers. Application Streptomyces sp. SP isolate with application time before planting showed the best results compared to other treatments. Keywords: Application Time, Fusarium sp., Streptomyces sp.  INTISARI                                                 Penyakit layu fusarium pada tanaman cabai merah disebabkan oleh jamur patogen Fusarium sp. Penyakit ini tergolong ke dalam penyakit penting tanaman cabai merah karena dapat menyebabkan kerusakan hingga 50%. Salah satu Upaya dalam mengatasi penyakit layu fusarium adalah menggunakan agensia hayati Streptomyces sp. yang berasal dari lahan bawang merah dan tomat di Pare, Kediri. Tujuan dari penelitian ini adalah untuk mengetahui mengetahui pengaruh apikasi Streptomyces sp. terhadap penyakit layu fusarium pada tanaman cabai merah dengan berbagai macam waktu aplikasi. Penelitian ini menggunakan Rancangan Acak Lengkap Faktorial dengan perlakuan jenis isolate SP dan BMP, serta perlakuan waktu aplikasi sebelum tanam, bersamaan tanam dan setelah tanam. Masing-masing perlakuan diulang sebanyak 5 kali dan diberikan 5 unit tanaman pada masing-masing ulangan. Parameter pengamatan dilakukan terhadap masa inkubasi, keparahan penyakit, tinggi tanaman, jumlah daun, umur berbunga dan jumlah bunga. Aplikasi Streptomyces sp. isolat SP dengan waktu aplikasi sebelum tanam menunjukkan hasil terbaik dibandingkan dengan perlakuan yang lain. Kata kunci: Waktu Aplikasi, Fusarium sp., Streptomyces sp. 
Studi Literasi Potensi Trichoderma spp. sebagai Jamur Entomopatogen Ummah, Robithotul; Suryaminarsih, Penta
Exact Papers in Compilation (EPiC) Vol 5 No 1 (2023): Februari 2023
Publisher : Lembaga Penelitian Pengabdian Masyarakat Universitas KH. A. Wahab Hasbullah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32764/epic.v5i1.888

Abstract

Pengendalihan hayati ini dapat digunakan untuk mengendalikan serangga hama. Salahsatunya menggunakan jamur entomopatogen berupa Trichoderma spp. Jamur entomopatogen adalah salah satu parasit alami ini. Jamur entomopatogen merupakan salah satu jamur yang bersifat heterotrof. Karena sifat heterotroph jamur entomopatogen hidup sebagai parasit pada serangga hama. Karakterisasinya dapat dikenali salah satunya melalui karakter makroskopis dan juga mikroskopisnya. Beberapa spesiesnya yaitu T. hamantum, T. koningii, T. harzianum, T. polysporum, dan T. Mekanisme kerjanya dengan hifa jamur tumbuh di perut bagian bawah larva dan menutupi ostiola pernapasan aureoviride. Jamur bersifat saprofit menghasilkan aktivitas enzimatik yang lebih luas dibandingkan dengan patogen yang menghasilkan subset enzim yang mencerminkan ceruk ekologisnya. Perbanyakan massal Trichoderma spp. dapat menggunakan media Potato Dextrose Agar (PDA) dan perbanyakan pada media jagung serta beras. Trichoderma spp. sebagai entomopatogen memiliki kelebihan dan kekurangan.
Faktor Efektivitas Penggunaan Nematoda Entomopatogen Syahrok, Siti Fatimatus; Suryaminarsih, Penta
Exact Papers in Compilation (EPiC) Vol 5 No 1 (2023): Februari 2023
Publisher : Lembaga Penelitian Pengabdian Masyarakat Universitas KH. A. Wahab Hasbullah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32764/epic.v5i1.895

Abstract

Pengendalian secara hayati menggunakan nematoda entomopatogen merupakan salah satu upaya dalam pengendalian hama ramah lingkungan serta mampu menjaga ekosistem lingkungan tanaman. Mekanisme patogenesitas NEP melalui bakteri yang berasosiasi dalam fase juvenil infektif sehingga mampu membunuh serangga hama. Keberadaan nematoda entomopatogen dipengaruhi oleh faktor biotik dan abiotik. Faktor biotik meliputi pemilihan jenis spesies, jumlah spesies dan fase hidup. Sedangkan factor abiotik meliputi kondisi lingkungan, kelembaban tanah, suhu tanah, pH tanah, dan kandungan pupuk dan pestisida kimia dalam tanah. Perbanyakan secara konvensional yang efisien tenaga, waktu dan biaya yaitu secara in vivo dengan teknik baiting menggunakan larva Tenebrio molitor. Teknik aplikasi NEP dapat dilakukan dengan berbagai cara penyemprotan dan irigasi, baik menggunakan irigasi tetes, penyemprotan tekanan, penyemprotan kabut dan elektrostatis. Faktor yang perlu diperhatikan mengenai tekanan, jenis nosel dan hama sasaran. Salah satu faktor efektifitas NEP adalah pengembangan formulasi dosis yang efektif. Dosis terbaik pada konsentrasi Heterorhabditis sp. 400 JI/ml tercapai setelah 37,96 jam setelah aplikasi. Pengaplikasian Heterorhabditis sp. pada larva Spodoptera litura menimbulkan mortalitas sebesar 43% setelah 24 jam aplikasi. Pengembangan formulasi dosis harus terus dilakukan dikarenakan setiap spesies NEP memiliki kemampuan patogenesitas berbeda dan tergantung pada hama sasaran.
Potential of Streptomyces spp. From Peanut Plant Soil as an Entomopathogen of Pests Spodoptera Litura Ferdianto Eko Wicaksono; Wiwin Windriyanti; Penta Suryaminarsih
Demeter: Journal of Farming and Agriculture Vol. 2 No. 1 (2024): March 2024
Publisher : CV. Media Digital Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58905/demeter.v2i1.290

Abstract

Peanuts are agricultural commodities that have high economic value and play an important role in meeting human needs, namely as a source of vegetable protein, oil and other nutrients, but domestic peanut production has not been able to meet the needs of the Indonesian people. The national demand for peanuts reaches 856.1 thousand tons per year, and the average consumption of peeled peanuts is 0.32 kg per capita every year. [1] In 2013 peanut production was 701,680 tons, then there was a decrease in production in 2014 to 638,896 tons, and continued until 2015 to 605,449 tons, thus showing a 13.7% decrease that occurred from 2013 to 2015. Low productivity is caused by various factors, one of which is the attack of pests and plant diseases. This study aims to determine the type of Streptomyces spp. Microbes with high chitinolytic content that can control armyworm pests (S.litura) on peanut plants. and influence of biological control agency Streptomyces spp as an entomopathogen of insect pests S. litura. This study was able to improve understanding of the influence of the biological control agency Streptomyces spp as an entomopathogen of S. litura insect pests to protect plants in the future. The results of the exploration of Streptomyces sp. acquired Colonies are irregular and branched coccus-shaped. The colony is tightly attached to the GNA medium, wavy white with a notched surface and a starchy texture. In addition, it has the largest chitinase enzyme content with a chitin zone diameter of 3 cm. PCR test Based on BLAST® result tabulation data that isolates belong to the genus Streptomyces sp. with an identity percentage of 99.86% with Streptomyces sp. VEL gene 17 to 16 rRNA, partial sequence with access code AB914463.2 The highest mortality was in the treatment (S0 RP K3) and (S1 RP K3) there was 60% this showed that with the administration of Streptomyces sp. With a concentration of 75% can cause very high mortality with abdominal contact poison in S. Litura pests
Potensi Streptomyces sp. Terhadap Penurunan Virulensi Bakteri Ralstonia solanacearum dalam Skala In vitro Sa'adah, Fatimah Lailatus; Suryaminarsih, Penta; Prasetyawati, Endang Triwahyu
Jurnal Agroekoteknologi Vol 16, No 1 (2024)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33512/jur.agroekotetek.v16i1.23238

Abstract

The demand for chili peppers in Indonesia continues to increase, but in 2021 there was a decrease in chili production. One of the main causes is pathogen attack, especially Ralstonia solanacearum which causes bacterial wilt disease in chili plants. This study aims to evaluate the potential of Streptomyces sp. isolates BMP and TMP to reduce the virulence of R. solanacearum. The research was conducted through a series of laboratory experiments, including an inhibition test with double-layer method and virulence reduction test on Triphenyl Tetrazolium Chloride (TZC) media. The results showed that the TMP isolate was able to produce an inhibition zone of 1.5 cm2 while the BMP isolate showed no inhibition zone. In addition, this study also evaluated the virulence reduction of R. solanacearum due to the treatment of Streptomyces spp. The virulence reduction test showed that TMP isolates were able to reduce virulence by 70% while BMP isolates were able to reduce the virulence of R. solanacearum by 4%. These findings provide new insights into the development of R. solanacearum control strategies. In conclusion, isolates of Streptomyces sp. especially BMP showed potential in reducing the virulence of R. solanacearum, thus providing new hope in efforts to increase chili production sustainably.
Application of Streptomyces sp. and Trichoderma sp. for Promoting Generative Plants Growth of Cherry Tomato (Lycopersicum cerasiformae Mill.) Nawaal, Najvania; Guniarti, Guniarti; Moeljani, Ida Retno; Suryaminarsih, Penta
PLANTA TROPIKA Vol 10, No 2 (2022)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v10i2.11706

Abstract

Production of cherry tomatoes in Indonesia is still low, which might be due to the inappropriate planting and maintenance processes. This research applied biological agent microorganisms Streptomyces sp. and Trichoderma sp. as Plant Growth Promoting Microorganisms (PGPM) in sustainable agricultural systems. This study aimed to determine the effect of the concentration of microorganisms Streptomyces sp. and Trichoderma sp. on the growth and production of cherry tomato plants on the polybag scale. The study was arranged with different concentrations of microorganisms Streptomyces sp. and Trichoderma sp. These concentration applied consisted of 1 : 0 ; 0 : 1 ; 2: 2 ; 3 ; 1 and without PGPM, each repeated four times. The results showed that the treatment of PGPM Streptomyces sp. and without Trichoderma sp. (1:0) resulted in the shortest flowering period (33.99 days after planting). Meanwhile, the treatment without Streptomyces sp. and Trichoderma sp. (0 : 1) produced the highest solid weight fruit (69.82 grams/plant).
Carrier Materials Differences on Bioencapsulation Formulation Affect Bacillus sp. Isolates Wuryandari, Yenny; Suryaminarsih, Penta; Lestari, Safira Rizka
Nusantara Science and Technology Proceedings 8th International Seminar of Research Month 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4162

Abstract

Biological agent Bacillus sp. Based on the results of previous research, direct use of isolated microorganisms BCZ14, BCZ16, and BCZ20 was able to suppress Fusarium wilt disease. However, Bacillus sp. formulations in suspension form are less effective when applied in the field. This research focuses on analyzing the viability of three isolates of Bacillus sp. against several combinations of bioencapsulation coating materials. The experimental design used was a completely randomized design with a combination of treatment of three isolates and three types of coating materials, namely sodium alginate (B1), carrageenan (B2), and chitosan (B3) each 2%. Each treatment combination was repeated five times. Based on the experiments that have been carried out, the bioencapsulation formulation using 2% chitosan coating material was able to maintain the viability of the three isolates of Bacillus sp. BCZ14, BCZ16, and BCZ20. The population density of the three isolates of Bacillus sp. persisted consistently from the first week to the last week of observation. Therefore, the 2% chitosan coating material can be said to be able to maintain the viability of Bacillus sp. within six weeks.
Carrier Materials Differences on Bioencapsulation Formulation Affect Bacillus sp. Isolates Yenny Wuryandari; Penta Suryaminarsih; Safira Rizka Lestari
Nusantara Science and Technology Proceedings 8th International Seminar of Research Month 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4162

Abstract

Biological agent Bacillus sp. Based on the results of previous research, direct use of isolated microorganisms BCZ14, BCZ16, and BCZ20 was able to suppress Fusarium wilt disease. However, Bacillus sp. formulations in suspension form are less effective when applied in the field. This research focuses on analyzing the viability of three isolates of Bacillus sp. against several combinations of bioencapsulation coating materials. The experimental design used was a completely randomized design with a combination of treatment of three isolates and three types of coating materials, namely sodium alginate (B1), carrageenan (B2), and chitosan (B3) each 2%. Each treatment combination was repeated five times. Based on the experiments that have been carried out, the bioencapsulation formulation using 2% chitosan coating material was able to maintain the viability of the three isolates of Bacillus sp. BCZ14, BCZ16, and BCZ20. The population density of the three isolates of Bacillus sp. persisted consistently from the first week to the last week of observation. Therefore, the 2% chitosan coating material can be said to be able to maintain the viability of Bacillus sp. within six weeks.
Efikasi Formulasi Granular Kombinasi Trichoderma sp. dan Ekstrak Daun Bandotan terhadap Sclerotium rolfsii pada Porang (Amorphophallus oncophyllus) Raniah, Nabilla Nur; Nirwanto, Hery; Suryaminarsih, Penta
BIOEDUSCIENCE Vol 8 No 3 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/14880

Abstract

Background: Amorphophallus oncophyllus is a tuber plant that is beneficial for various industrial sectors, but in its cultivation, it is often affected by the pathogen Sclerotium rolfsii, requiring control measures. Control efforts can be carried out using a combination of the biological agent Trichoderma sp. and the plant pesticide made from bandotan leaf extract. The combined control application can be formulated in a solid granular form. Method: This research was conducted both in vitro and in vivo. In vitro, a Completely Randomized Design (RAL) was used with the combination of Trichoderma sp. and bandotan leaf extract, divided into four levels: 0% (TB0), 5% (TB5), 10% (TB10), and 15% (TB15). In vivo, a 2-factor RAL was applied. The first factor was the concentration of the rice flour carrier material in four levels: 0% (B1), 25% (B2), 50% (B3), and 75% (B4). The second factor was the storage duration of the granular formulation, consisting of 0 weeks (M1), 3 weeks (M2), and 5 weeks (M3). Result: The study's results showed that the granular combination of Trichoderma sp. and 10% bandotan leaf extract reduced S. rolfsii infection by 64.25% and stimulated the growth of porang plant height. Conclusion: The treatment with the granular combination of Trichoderma sp. and 10% bandotan leaf extract, adding 25% rice flour and a storage period of 0-3 weeks, showed the best effect on the porang plant seedlings.
Co-Authors Abifah, Serlia Nur Aisyah Lulu Hariyanto Alifta Wiranthi Anita, Tri Yuli Ardhia Pramesti Cahyani Arga Dwi Indrawan Arika Purnawati Chairunnisa Faza Nabillah Diah Budi Kusumawati Didin Julia Trisnawati Difa Eka Pranoto Dinda Rizka Rahayu Dita Megasari Dwi Lestari Elly Syafriani Erdiansyah, Mochamad Bima Erlangga Dwi Fachruddin Fadila Suryandika Ferdianto Eko Wicaksono Firdi Ihza Rochman Guniarti, Guniarti Gusdino Hasyidan Gusdino Hasyidan Haryani, Wiwik Sri Hery Nirwanto Hery Nirwanto, Hery Ida Retno Moeljani Iis Purnamawati Ika Nur Fitriana Ika Nur Fitriana Indah Sari Dwi Agustin Indah Sari Dwi Agustin Jati Laksono, Roy Juniawan Laksamana Agadhia Raharjo Lestari, Safira Rizka Maemunah Mahfud, Nur Aini Maroeto Maroeto Moch Sodiq Nawaal, Najvania Nolla Dwi Elvira Noni Rahmadhini Nugraha Iqbal Avrianto Nur Syiami, Aning Pangesti Nugrahani Prasetyawati, Endang Triwahyu Prasetyawati, Endang Triwahyu Pribadi, Didik Utomo Putranto Sasikirono Ramdan Hidayat Raniah, Nabilla Nur Rateh Lailatul Risdiyanti Rateh Lailatul Risdiyanti Rizky Ika Noviyanti Sa'adah, Fatimah Lailatus Safira Rizka Lestari Salfi Mauludhiea Firli Savira Aprilia Ekayanti Serlia Nur Abifah Setyo Budi Santoso Siti Fatimatus Syahrok Sri Wiyatiningsih Sri Wiyatiningsih Syahrok, Siti Fatimatus Tri MUJOKO Tri Mujoko Ummah, Robithotul Wanti Mindari Wiludjeng Widajati Wiludjeng Widayati Wiludjeng Widiyati Windriyanti, Wiwin Wiwik Sri Harijani Wiwik Sri Harijani Yenny WURYANDARI