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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran TREUBIA Jurnal Sains Teknologi dan Lingkungan (JSTL) Jurnal Penelitian Pendidikan IPA (JPPIPA) Communications in Science and Technology Jurnal Abdi Insani Bioscientist : Jurnal Ilmiah Biologi Samota Journal of Biological Sciences (SJBIOS)
Galuh Tresnani
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
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Fishmeal-Based Media Supports Growth and Endospore Production of Locally-Isolated Lysinnibacillus sphaericus and Induces its Toxicity to 3rd Instar Aedes aegypti Larvae in Laboratory Conditions Suryadi, Bambang Fajar; Mustikasari, Ika; Annisa, Zuriatun; Sarkono; Tresnani, Galuh
Jurnal Penelitian Pendidikan IPA Vol 10 No 7 (2024): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i7.7361

Abstract

The aim of this study was to determine whether fishmeal-based media could be used to grow L. sphaericus and induce its toxicity against Aedes aegypti larvae. Three concentrations (10, 20 and 30%) of fishmeal-based media were used to grow L. sphaericus isolate Bs2-1-2. Cell growth and endospore production were observed every 12 hours for 72 hours. The lethal concentration was measured every 24 hours for 72 hours of fermentation. The highest cell concentration was found in L. sphaericus grown on the media with 30% fishmeal concentration (3.03x1008 cells/mL), followed by 20% concentration (2.43x1008 cells/mL) and the lowest at 10% concentration (2.20x1008 cells/mL). At the end of fermentation, the highest concentration was found in L. sphaericus grown on 30% fishmeal-based media (1.51x1008 cells/mL), followed by 20% media (6.95x1007 cells/mL) and 10% media (3.21x1007 cells/mL). After 72-hour incubation, the highest endospore concentration was achieved by L. sphaericus grown on 20% (2.51x1008 cells/mL) and 10% (2.19x1008 cells/mL) fishmeal-based media. Initial larval toxicity of L. sphaericus showed the highest mortality on 20 and 30% fishmeal-based media (both reaching 53.33%), while 10% fishmeal-based media gave only 26.67% larval mortality. The LC50 value at 72 hours was achieved by L. sphaericus cultured on 30% fishmeal-based media (2.47 x 1008 cells/mL), followed by 20% concentration (4.82 x 1008 cells/mL) and 10% concentration (9.01 x 1009 cells/mL). The conclusion of this study was all concentrations of fishmeal-based media could support cell growth, endospore production and larval toxicity induction of L. sphaericus.
Jenis dan Kelimpahan Nyamuk di Resort Kembang Kuning, Taman Nasional Gunung Rinjani Tresnani, Galuh; Hadi, Islamul; Zamroni, Yuliadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18519

Abstract

This study aims to identify mosquito species as disease vectors and count their abundance in the Kembang Kuning Resort area, Mount Rinjani National Park. Larval samples were collected by traversing a 1.5 km trail. Samples were randomly collected from containers or water bodies containing larvae. The larval samples obtained from each 100 ml scoop were counted and identified by species. Some larval samples were reared to adulthood. Successfully hatched adult mosquitoes are identified by their species. Relative abundance is calculated from the number of larvae per scoop, and the container index (CI) value is calculated from the number of containers found positive for larvae. The research findings revealed 10 species belonging to family Culicidae and according to Rikhus Vektora (B2P2VRP, 2016), 9 of these species are vectors. The highest relative abundance values were found for the species Aedes albopictus (36.9%) and Culex quinquefasciatus (29.4%). The CI value for mosquitoes at Kembang Kuning Resort was 43.2%. The conclusion of this study is that Kembang Kuning Resort has 10 mosquito species, with the highest relative abundance for Aedes albopictus and Culex quinquefasciatus. A CI value greater than 5 indicates a relatively high risk of vector-borne disease transmission in this area.
Co-Authors Annisa, Zuriatun Atikah, Tika Dewi Budhy Kurniawan Damiyati, Siska Yunita Faturrahman Fitriana, Yuli S. Gito Hadiprayitno Hidayat, Hilal Aisyah I WAYAN SUANA Ika Mustikasari, Ika ISLAMUL HADI Kartika Dewi Kusuma, AA Ngurah Nara Maharadatunkamsi Maharadatunkamsi, Maharadatunkamsi Martini, Rima Muhammad Rijal Alfian Nana Supriatna Nurlaila Mubarokah Nurul Inayah Permatasari, Baiq Dewi Pita, Baiq Nuraini Ayu Pratama, Iman Surya Pratama, Iman Surya Putri, Vita Septiana Sulaiman Sadewo, Imran Saputri, Ferza Ayu Sigit Wiantoro, Sigit Solehah, Kurnia Supardiono, Supardiono Suryadi, Bambang Fajar Taqwim, Ahsani Yuliadi Zamroni Yuni Apriyanti, Yuni