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All Journal Media Veteriner Jurnal Veteriner Indonesia Medicus Veterinus Majalah Kedokteran Bandung ARSHI Veterinary Letters Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences
Iwan Harjono Utama
Fakultas Kedokteran Hewan, Universitas Udayana, Denpasar Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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IDENTIFIKASI Escherichia coli O157:H7 DARI FESES AYAM DAN UJI PROFIL HEMOLISISNYA PADA MEDIA AGAR DARAH I Wayan Suardana; Iwan Harjono Utama; Michael Haryadi Wibowo
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.278 KB) | DOI: 10.21157/j.ked.hewan.v8i1.1236

Abstract

Penelitian ini bertujuan melakukan isolasi dan identifikasi serotipe lokal Escherichia coli (E. coli) O157:H7 dan uji profil hemolisisnya pada media agar darah. Isolasi bakteri dilakukan dengan media eosin methylene blue agar (EMBA), dilanjutkan dengan identifikasi pada media selektif sorbitol MacConkey agar (SMAC) dan uji konfirmasi menggunakan uji aglutinasi lateks O157 serta uji antiserum H7 sebagai konfirmasi akhir dari E. coli O157:H7. Gambaran hemolisis diuji dengan menumbuhkan isolat pada media agar darah domba. Hasil penelitian menunjukkan bahwa 7 isolat (8,54%) dari 82 sampel feses ayam teridentifikasi E. coli O157:H7 dan memperlihatkan profil enterohemolisis seperti halnya isolat kontrol ATCC 43894. Dari hasil penelitian disimpulkan bahwa isolat lokal E. coli O157:H7 hasil isolasi dari feses ayam diketahui memiliki patogenitas yang tinggi terkait dengan dihasilkannya enterohemolisin yang merupakan marka/penanda kemampuan dari isolat untuk menghasilkan faktor virulensi Shiga like toxin.
Antimicrobial Susceptibility Patterns And DNA Plasmid Profiles Of Escherichia Coli O157:H7 Isolated From Feces Of Chicken I Wayan Suardana; Iwan Harjono Utama; Michael Haryadi Wibowo
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p01

Abstract

Escherichia coli O157:H7 has been identified as one of the most devastating microorganism causing diseases in human. The infection by this bacteria causes a wide spectrum of clinical manifestations ranging from asymptomatic, diarrhea or bloody diarrhea, up to serious clinical conditions such as hemorrhagic colitis, and hemolytic uremic syndrome. Poultry is known as one of its reservoir besides cattle and pig. Moreover, the use of antibiotic in animal feed as growth promoters is common worldwide. Due to its potential threat to public health, sensitivity test against various antibiotics and studying the plasmid DNA profiles are necessary. The study was initiated by cultivation of isolates, followed by test of antimicrobial susceptibility against various antibiotics and then by analyzing the plasmid DNA profiles. Results of study showed that among of 7 E. coli O157:H7 local isolates originated from chicken feces, as many as 42.9%; 14.3%; 14.3%; and 14.3% were resistant to 2; 3; 4; and 5 various of antibiotics, respectively. Base on the type of antibiotic, 85.7%; 71.4%; and 42.9% showed resistance to Methicillin, Penicillin G, Doxycycline and Streptomycin. Plasmid DNA of each isolates showed characters variation too, ranging from 2 to 4 bands with molecular weight ranging from 19.457 bp; 15.137 bp, 5.749 bp, 4.118 bp, 3.500 bp, 2.957 bp and 2.024 bp. Results of study indicated that local isolates of E. coli O157:H7 isolated from chicken showed multiple antibiotic resistance against various antibiotics, as well as its plasmid DNA profiles.
Molecular Analysis of Lactic Acid Bacteria SR4 Strain Isolated from Gastric’s Juice of Bali Cattle Iwan Harjono Utama; I Wayan Suardana
Journal of Veterinary and Animal Sciences Vol 4 No 2 (2021)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2021.v04.i02.p03

Abstract

Lactic acid bacteria SR4 strain is known to have potential as a probiotic source according to the previous study. The isolate derived from the gastric’s juice of bali cattle has characteristics such as its resistance to growth at low pH, namely pH 2, 3, and 4, its tolerance to bile concentration of 0.6 mM, and its inhibition against Gram-positive and negative bacteria. On the other hand, a complete molecular analysis of the isolate has never been carried out. Based on these considerations, the molecular analysis of the LAB SR4 strain becomes interesting to present. The study was initiated by cultivating LAB SR4 isolate on an MRS broth medium. The isolate was further confirmed molecularly through analysis of the 16S rRNA gene which was initiated by isolating genomic DNA followed by PCR reaction using universal primers 27F and 1492R. The PCR product of the 16S rRNA gene was sequenced and further analyzed using the MEGA 5.2 software. The data were then analyzed descriptively and presented in the form of tables or figures. The results showed that the LAB strain SR4 was identified as a Pediococcus pentosaceus. This strain potentially to develops as a source of bacteriocin and it can be applied as a bio preservative, improvement of the meat taste, inhibition of inflammation, and also as antagonism of cancer.
IDENTIFIKASI Escherichia coli O157:H7 DARI FESES AYAM DAN UJI PROFIL HEMOLISISNYA PADA MEDIA AGAR DARAH Suardana, I Wayan; Utama, Iwan Harjono; Wibowo, Michael Haryadi
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1236

Abstract

Penelitian ini bertujuan melakukan isolasi dan identifikasi serotipe lokal Escherichia coli (E. coli) O157:H7 dan uji profil hemolisisnya pada media agar darah. Isolasi bakteri dilakukan dengan media eosin methylene blue agar (EMBA), dilanjutkan dengan identifikasi pada media selektif sorbitol MacConkey agar (SMAC) dan uji konfirmasi menggunakan uji aglutinasi lateks O157 serta uji antiserum H7 sebagai konfirmasi akhir dari E. coli O157:H7. Gambaran hemolisis diuji dengan menumbuhkan isolat pada media agar darah domba. Hasil penelitian menunjukkan bahwa 7 isolat (8,54%) dari 82 sampel feses ayam teridentifikasi E. coli O157:H7 dan memperlihatkan profil enterohemolisis seperti halnya isolat kontrol ATCC 43894. Dari hasil penelitian disimpulkan bahwa isolat lokal E. coli O157:H7 hasil isolasi dari feses ayam diketahui memiliki patogenitas yang tinggi terkait dengan dihasilkannya enterohemolisin yang merupakan marka/penanda kemampuan dari isolat untuk menghasilkan faktor virulensi Shiga like toxin.
Co-Authors Achoiro Wati Rasid Agnes Endang Tri H Ahmad Rohmadhon Holifatullah Aida Lousie Tenden Rompis Aisjah Girindra Anak Agung Gde Oka Dharmayudha Anak Agung Sagung Kendran Anggi Windo Marta, Anggi Windo Azmil Umur, Azmil Baiq Yunita Arisandi Bambang Pontjo Priosoeryanto Brigitta Cynthia Sida Pello, Brigitta Cynthia Candrayani, Putu Prema Chanda Preanger, Chanda Dewa Ayu Dwita Karmi Drevani Angelika Sachio DWI SURYANTO Ekklesia Prasetya El vira Elfani Sarah Faradina Endhie D. Setiawan ERMITA TINTING BUNTU Eva Candra Ardia, Eva Candra Evi Marieti Hutagalung Fachriyan Hasmi Pasaribu Fransiska Septanila Pratiwi, Fransiska Septanila Gatut Ashadi Gusti Agung Rama Wiratama Putra Heparandita, Ananda Agung Dextra I Gusti Agung I Gusti Agung Ayu Suartini I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Badiwangsa I Ketut Berata I Made Kardena I Made Merdana I Nyoman Suarsana I Nyoman Suartha I Nyoman Sulabda I Wayan Puspa Ari Laxmi I Wayan Suardana I wayan Teguh Wibawan I Wayan Wirata Ida Bagus Komang Ardana Ida Bagus Oka Winaya Iga Prassetyo Adji, Iga Prassetyo Juli Yanti, Juli Karami, Citra Kartikasari, Citra Dewi Ketut Berata Ketut Suartini Luh Made Sudimartini MARIA BINTANG Michael Haryadi Wibowo N iMade Sutari Dewi Ni Luh Eka Setiasih Ni Luh Watiniasih Nindya Kusumawati Nuria Fitrianti Putri Perayadhista, Komang Tri Mywisti Permana, Rizky Pratama, Dimas Andi PRATIWI DEVI GM Pratiwi, Zulva Hanif Putra, Komang Darma Yudha Putri Virgania Putu Ayu Sisyawati Putriningsih Reggy Raisa Tangalayuk Reni, Ida Yuni Erdia Ridwan, Isabella Anjari S Siswanto Samsuri Samsuri Setiyowati, Asri Sherliyanti Maria Sene Sri Kayati Widyastuti Suharsono, Hamong Tjokorda Sari Nindhia Tyas Pandieka Yoga Widarta Dwi Kusuma Yanne Yanse Rumlaklak Zaidany Alfanandyah, Zaidany