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Effects of Tapak Dara Leaves Extract on Kidney Histology of Aspartame-Induced Mice Utami, Eva Tyas; Kurnia, Indah Salsabila; Mahriani, Mahriani
Biosfer: Jurnal Tadris Biologi Vol 12 No 2 (2021): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v12i2.10007

Abstract

This study aimed to determine the effect of Catharanthus roseus leaves extract on kidney histology of mice aspartame-induced. Mice were divided into 4 groups, namely the negative control group (K-) without treatment, the positive control (K+) given aspartame 0.02 mg/day, the dose group 1 (D1) was given aspartame 0.02 mg/day and continued with the C. roseus leaves extract at a dose of 0.42 mg/kg. Group 2 (D2) was given aspartame 0.02 mg/day followed by C. roseus leaf extract at a dose of 0.84 mg/kg BW. IP administered aspartame for 14 days, and C. roseus leaves extract was administered by gavage for 12 days. The data obtained were analyzed by One Way ANOVA and DMRT test with a significance level of 0.05. The results showed that the induction of aspartame at a dose of 0.02 mg/day in mice increased the average number of proximal tubular necrosis cells. Catharanthus roseus leaves extract administration at 0.42 mg/kg BW and 0.84 mg/kg BW for 12 days can reduce the number of proximal tubular necrosis cells in aspartame-induced mice. Thus it can be concluded that the administration of C. roseus leaves extract can reduce the proximal kidney tubules necrosis but has not been able to reach normal conditions yet.
Counseling on The Importance of Maintaining Women's Reproductive Health In Arjasa Village, Arjasa Sub-District, Jember District Fajariyah, Susantin; Utami, Eva Tyas; Nihayah, Husnatun
Jurnal Inovasi Sains dan Teknologi untuk Masyarakat Vol. 3 No. 1 (2025): Mei
Publisher : Faculty of Mathematics and Natural Sciences, University of Jember. Jl. Kalimantan No.37, Krajan Timur, Jemberlor, Kec. Sumbersari, Jember Regency, East Java 68121

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/instem.v3i1.5487

Abstract

Women's reproductive health is an important aspect in maintaining community welfare, especially in preventing various diseases related to the reproductive organs. This counseling aims to increase the understanding and awareness of the community, especially mothers in Arjasa Village, about the importance of maintaining reproductive health. The method used in this activity is in the form of interactive counseling and discussion. The stages of service activities carried out: situation analysis, coordination with the village government, and implementation of activities. This activity involved 35 female participants aged 18-35 years, the majority of whom were housewives. The counseling material includes the definition of reproductive health, factors that affect it, common diseases, as well as prevention and early detection measures. The results of the activity showed that the participants had high enthusiasm in participating in the counseling, which could be seen from the active interaction during the question and answer session. The results of the activity showed a fairly good understanding of the counseling participants as seen from the enthusiasm of the participants in participating in the counseling and discussion sessions. The main obstacle in this activity was the limited time which caused some materials to be delivered briefly. Overall, this counseling has a positive impact in increasing awareness of women's reproductive health. It is expected that follow-up in the form of collaboration with village health cadres can continue to be carried out to maintain the reproductive health of the local community.
The Integrated Local Chicken Farming in Enhancing the Value of Domestic Waste as Empowering BSU ASRI Waste Banks Lelono, Asmoro; Nur Asyia Alfiyani; Intani, Rini Nur; Nurdian, Yudha; Utami, Eva Tyas; Wijaya, Rizky Surya; Aurellia, Naurah Septa
Jurnal Inovasi Sains dan Teknologi untuk Masyarakat Vol. 3 No. 1 (2025): Mei
Publisher : Faculty of Mathematics and Natural Sciences, University of Jember. Jl. Kalimantan No.37, Krajan Timur, Jemberlor, Kec. Sumbersari, Jember Regency, East Java 68121

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/instem.v3i1.5653

Abstract

Empowering of the Waste Bank Unit (BSU) through business diversification and optimization of waste utilization has become an urgent need in an effort to increase income and the sustainability of its activities. The Biology Department is collaborating with BSU ASRI, which has been operating since 2021 as a partner and has experience and knowledge in waste management, striving to applicate research results. The Zoological Integrated and Research Application (ZIRA) Research Group, with an interest in native chicken farming research in the field of Avian Immunity, is attempting to implement a model of native chicken farming to utilize organic waste that was previously used as feed for Black Soldier Fly (BSF) maggots. Previously, BSU ASRI utilized organic waste for raising BSF maggots as protein-rich animal feed. The methods used were: incubation of a ready-to-produce chicken farming model by building coops and managing their maintenance, incubation of household-scale farming by providing 1-month-old chicks to BSU members, and independent chick production using a small-scale electric incubator. The results of the activities showed a positive response from the community, as seen from their willingness to raise chickens and the beginning of its adoption in several households. Another benefit is the use of chicken livestock products, starting from ready-to-consume eggs and manure, as well as the utilization of maggot production as part of organic waste utilization.
Using the Benthic Macroinvertebrates Species Diversity Index to Determine the Water Quality of Antirogo River, Jember Regency Wimbaningrum, Retno; Utami, Eva Tyas; Agustina, Rizka; Setiawan, Rendy
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.53698

Abstract

The Antirogo river, as one of the rivers across the Jember regency, East Java, plays an important role and is used for various activities such as agriculture, domestic, and industrial activities. These anthropogenic activities could affect the water quality of the river and the biota within. A group of river biota affected by anthropogenic activities is the benthic macroinvertebrates. Benthic macroinvertebrates can be used as bioindicators of river water quality. This study aimed to assess the water quality of the Antirogo River using the Shannon Wiener species diversity index (H') based on benthic macroinvertebrate data. Benthic macroinvertebrate sampling was conducted using a Surber net at three research stations. This research found 1703 individuals of benthic macroinvertebrates, which were classified into 16 families, 10 orders, and five classes. The most abundant family was the Heptageniidae, while the least abundant was the Simuliidae. The benthic macroinvertebrates' diversity index (H’) value was 1.67, with the evenness index (E) value at 0.60. Based on the diversity index (H’) value, the water quality in this river was lightly polluted with pollutants that can be in the form of solid materials and toxic compounds.
Partial Analysis of Probiotic Character of Lactic Acid Bacteria from the Intestines of Broilers Supplemented with Fish Protein Hydrolysate (FPH) Nashrullah, Shafa; Utami, Eva Tyas; Purniasari, Fina Yunita; Anggitasari, Dhanti Fatma; Utarti, Esti
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.53701

Abstract

This study aims to analyze partial characteristics of probiotics lactic acid bacteria (LAB) from broiler intestines supplemented with fish protein hydrolysate (FPH) 2% (v/w) in feed. A total of 17 LAB isolates were successfully isolated and characterized morphologically and biochemically (catalase test). The isolate showed diverse morphological and biochemical properties, obtained as many as seven isolates that met the partial characteristics of probiotics, namely U10, U11, U13, U16, U31, U33, and U37 which were Gram-positive and no endospores and catalase formations were found. The tolerance test for acid pH and NaCl performed on the seven BAL isolates showed a diverse survival rate with U11 showing the highest tolerance at pH 2 (11.4%) and NaCl concentration up to 8% (32.4%). These findings highlight seven LAB isolates with partial probiotic properties, providing new insights into the development of probiotic candidates from broiler guts to improve gastrointestinal health and productivity.
Moringa oleifera gum effect on blood sugar levels and rat pancreas histology induced by Streptozotocin Utami, Eva Tyas; Fajariyah, Susantin; Lelono, Asmoro; Nihayah, Husnatun
JPBIO (Jurnal Pendidikan Biologi) Vol 9, No 1 (2024): April 2024
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v9i1.3184

Abstract

Moringa gum is a product that people can use as an anti-hyperglycemic agent. This study aimed to determine the effectiveness of Moringa gum in reducing blood sugar levels and improving the histology structure of the rat pancreas induced by streptozotocin (STZ). This study used 21 male rats divided into 3 groups including control, diabetes (STZ), and diabetes (STZ + 3% Moringa gum). STZ treatment at a single dose (45 mg/kg BW) was given intraperitoneally, while Moringa gum was given through drinking water for 2 weeks. Blood sugar levels were measured on days 0, 7th, 14th, 21st, and 28th. The results showed that  STZ administration induced diabetes in rats with blood sugar levels of 487 mg/dL on the 14th day.  Moringa gum administration was able to reduce blood sugar levels on days 21st and 28th respectively to 306 and 234 mg/dl. The histology structure of the pancreas of rats treated with gum also showed improvement. This study concludes that Moringa gum is effective in lowering blood sugar levels in rats even though it still does not show normal blood sugar levels, and can repair STZ-induced damage to the histology structure of the rat pancreas.
Lactic Acid Bacteria Isolated From Digestive Tract of Broilers Treated with Fish Protein Hydrolysate Utarti, Esti; Utami, Eva Tyas; Sjaifullah, Achmad; Handayani, Wuryanti; Belkis, Malika; Medayani, Rani Dian
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.38422

Abstract

The composition of the feed plays a role in stimulating the activity of the microbiota in the gastrointestinal tract; therefore, the addition of fish protein hydrolysate (FPH) is suspected to influence the activity of microbiota, especially probiotics. Therefore, the presence of probiotics in the gastrointestinal tract affects the weight and quality of the broiler. This research aims to investigate the effect of administering fish protein hydrolysate as a dietary supplement on the composition of lactic acid bacteria (LAB), a potential probiotic candidate. This research was conducted in several stages, including the diversification of feeding broilers, the isolation and purification of LAB from the caecum and small intestine, primary characteristics, pathogenicity tests, and biochemical identification of LAB. Feed diversification was conducted by supplementing diets with 1%, 1.5%, and 2% FPH in 20-day-old for 7 days.. LAB from the small intestine and caecum samples were isolated on MRSA media by adding CaCO3. The Gram test, catalase test, and endospore staining test were carried out to characterize the suspected LAB primarily. The pathogenicity test was conducted by inoculating LAB on blood agar medium. Furthermore, biochemical tests are carried out using the KB020 kit. The results showed that the highest population of LAB in the small intestine (1.57 × 108 CFU/mL) was observed with 2% FPH supplementation. In comparison, the caecum yielded the highest population (1.22 × 108 CFU/mL) under 1.5% FPH. Giving 2% FPH  to broiler chicken feed provides a weight gain of 1.021 kg/head. The primary characteristics of the eight bacterial colony isolates suspected of being LAB were Gram-positive, catalase-negative, and did not form endospores. Eight LAB isolates of probiotic candidates were non-pathogenic as indicated by the occurrence of α-hemolysis and γ-hemolysis. Biochemical identification of probiotic candidates yielded four types of Lactobacillus, namely L. mucosae, L. frumenti, L. sanfranciscensis, and L. ferintoshensis. These LAB strains show promising probiotic potential for use as a feed additive in the broiler production system.
Mosquitoes' larval habitat characteristics and Aedes aegypti resistance status to malathion in Jember Nihayah, Husnatun; Purwatiningsih, Purwatiningsih; Utami, Eva Tyas; Fajariyah, Susantin; Lelono, Asmoro; Puspitasari, Reni; Rohmah, Hidayatur
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1037

Abstract

Water-holding containers serve as breeding sites for Aedes aegypti. The control of mosquito breeding sites and the use of household insecticides are some of the DHF control methods. The use of household insecticides over a long period can reduce the susceptibility of mosquitoes to these insecticides. This research aims to determine the mosquito's habitat characteristics and to determine the Aedes aegypti resistance status to malathion in Jember.  A total of 61 houses were surveyed, and the various types of containers encountered at the sampling locations were recorded. Larvae and pupae found at the sampling location are brought and hatched in the laboratory. Aedes aegypti was tested for resistance status to malathion insecticide using a CDC bottle bioassay and biochemical nonspecific esterase enzyme activity. A total of 140 containers were found in this study, and 36.43% of them are Aedes aegypti larvae/pupae habitat. Types of containers used as mosquito larval habitat are bottles/glasses, bathtubs, used basins/buckets, water storage buckets, trash cans, refrigerator water containers, used cans, gutters, livestock drinking water containers, fish ponds, plant pots, used livestock cages, used toilets, used jugs, used animal feeders, and used aquariums. The mortality of Aedes aegypti reached 42.3% (resistant). Meanwhile, the results of biochemical tests showed an increase in the activity of the nonspecific esterase enzyme. This study concludes that Aedes aegypti from Jember has been resistant to malathion insecticide