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All Journal Jurnal Bios Logos Jurnal Ilmu Dasar BERKALA SAINSTEK Biosfer: Jurnal Tadris Biologi UNEJ e-Proceeding Jurnal Biodjati BIOEDUKASI BIOEDUSCIENCE Jurnal Biologi Tropis Biota JPBIO (Jurnal Pendidikan Biologi) Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat SULUH: Jurnal Abdimas Bioscientist : Jurnal Ilmiah Biologi InSTEM Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Eva Tyas Utami
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Jember University, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Physics Social Sciences Other

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The Integrated Local Chicken Farming in Enhancing the Value of Domestic Waste as Empowering BSU ASRI Waste Banks Lelono, Asmoro; Nur Asyia Alfiyani; Intani, Rini Nur; Nurdian, Yudha; Utami, Eva Tyas; Wijaya, Rizky Surya; Aurellia, Naurah Septa
Jurnal Inovasi Sains dan Teknologi untuk Masyarakat Vol. 3 No. 1 (2025): Mei
Publisher : Faculty of Mathematics and Natural Sciences, University of Jember. Jl. Kalimantan No.37, Krajan Timur, Jemberlor, Kec. Sumbersari, Jember Regency, East Java 68121

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/instem.v3i1.5653

Abstract

Empowering of the Waste Bank Unit (BSU) through business diversification and optimization of waste utilization has become an urgent need in an effort to increase income and the sustainability of its activities. The Biology Department is collaborating with BSU ASRI, which has been operating since 2021 as a partner and has experience and knowledge in waste management, striving to applicate research results. The Zoological Integrated and Research Application (ZIRA) Research Group, with an interest in native chicken farming research in the field of Avian Immunity, is attempting to implement a model of native chicken farming to utilize organic waste that was previously used as feed for Black Soldier Fly (BSF) maggots. Previously, BSU ASRI utilized organic waste for raising BSF maggots as protein-rich animal feed. The methods used were: incubation of a ready-to-produce chicken farming model by building coops and managing their maintenance, incubation of household-scale farming by providing 1-month-old chicks to BSU members, and independent chick production using a small-scale electric incubator. The results of the activities showed a positive response from the community, as seen from their willingness to raise chickens and the beginning of its adoption in several households. Another benefit is the use of chicken livestock products, starting from ready-to-consume eggs and manure, as well as the utilization of maggot production as part of organic waste utilization.
Using the Benthic Macroinvertebrates Species Diversity Index to Determine the Water Quality of Antirogo River, Jember Regency Wimbaningrum, Retno; Utami, Eva Tyas; Agustina, Rizka; Setiawan, Rendy
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.53698

Abstract

The Antirogo river, as one of the rivers across the Jember regency, East Java, plays an important role and is used for various activities such as agriculture, domestic, and industrial activities. These anthropogenic activities could affect the water quality of the river and the biota within. A group of river biota affected by anthropogenic activities is the benthic macroinvertebrates. Benthic macroinvertebrates can be used as bioindicators of river water quality. This study aimed to assess the water quality of the Antirogo River using the Shannon Wiener species diversity index (H') based on benthic macroinvertebrate data. Benthic macroinvertebrate sampling was conducted using a Surber net at three research stations. This research found 1703 individuals of benthic macroinvertebrates, which were classified into 16 families, 10 orders, and five classes. The most abundant family was the Heptageniidae, while the least abundant was the Simuliidae. The benthic macroinvertebrates' diversity index (H’) value was 1.67, with the evenness index (E) value at 0.60. Based on the diversity index (H’) value, the water quality in this river was lightly polluted with pollutants that can be in the form of solid materials and toxic compounds.
Partial Analysis of Probiotic Character of Lactic Acid Bacteria from the Intestines of Broilers Supplemented with Fish Protein Hydrolysate (FPH) Nashrullah, Shafa; Utami, Eva Tyas; Purniasari, Fina Yunita; Anggitasari, Dhanti Fatma; Utarti, Esti
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.53701

Abstract

This study aims to analyze partial characteristics of probiotics lactic acid bacteria (LAB) from broiler intestines supplemented with fish protein hydrolysate (FPH) 2% (v/w) in feed. A total of 17 LAB isolates were successfully isolated and characterized morphologically and biochemically (catalase test). The isolate showed diverse morphological and biochemical properties, obtained as many as seven isolates that met the partial characteristics of probiotics, namely U10, U11, U13, U16, U31, U33, and U37 which were Gram-positive and no endospores and catalase formations were found. The tolerance test for acid pH and NaCl performed on the seven BAL isolates showed a diverse survival rate with U11 showing the highest tolerance at pH 2 (11.4%) and NaCl concentration up to 8% (32.4%). These findings highlight seven LAB isolates with partial probiotic properties, providing new insights into the development of probiotic candidates from broiler guts to improve gastrointestinal health and productivity.
Moringa oleifera gum effect on blood sugar levels and rat pancreas histology induced by Streptozotocin Utami, Eva Tyas; Fajariyah, Susantin; Lelono, Asmoro; Nihayah, Husnatun
JPBIO (Jurnal Pendidikan Biologi) Vol 9, No 1 (2024): April 2024
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v9i1.3184

Abstract

Moringa gum is a product that people can use as an anti-hyperglycemic agent. This study aimed to determine the effectiveness of Moringa gum in reducing blood sugar levels and improving the histology structure of the rat pancreas induced by streptozotocin (STZ). This study used 21 male rats divided into 3 groups including control, diabetes (STZ), and diabetes (STZ + 3% Moringa gum). STZ treatment at a single dose (45 mg/kg BW) was given intraperitoneally, while Moringa gum was given through drinking water for 2 weeks. Blood sugar levels were measured on days 0, 7th, 14th, 21st, and 28th. The results showed that  STZ administration induced diabetes in rats with blood sugar levels of 487 mg/dL on the 14th day.  Moringa gum administration was able to reduce blood sugar levels on days 21st and 28th respectively to 306 and 234 mg/dl. The histology structure of the pancreas of rats treated with gum also showed improvement. This study concludes that Moringa gum is effective in lowering blood sugar levels in rats even though it still does not show normal blood sugar levels, and can repair STZ-induced damage to the histology structure of the rat pancreas.
Lactic Acid Bacteria Isolated From Digestive Tract of Broilers Treated with Fish Protein Hydrolysate Utarti, Esti; Utami, Eva Tyas; Sjaifullah, Achmad; Handayani, Wuryanti; Belkis, Malika; Medayani, Rani Dian
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.38422

Abstract

The composition of the feed plays a role in stimulating the activity of the microbiota in the gastrointestinal tract; therefore, the addition of fish protein hydrolysate (FPH) is suspected to influence the activity of microbiota, especially probiotics. Therefore, the presence of probiotics in the gastrointestinal tract affects the weight and quality of the broiler. This research aims to investigate the effect of administering fish protein hydrolysate as a dietary supplement on the composition of lactic acid bacteria (LAB), a potential probiotic candidate. This research was conducted in several stages, including the diversification of feeding broilers, the isolation and purification of LAB from the caecum and small intestine, primary characteristics, pathogenicity tests, and biochemical identification of LAB. Feed diversification was conducted by supplementing diets with 1%, 1.5%, and 2% FPH in 20-day-old for 7 days.. LAB from the small intestine and caecum samples were isolated on MRSA media by adding CaCO3. The Gram test, catalase test, and endospore staining test were carried out to characterize the suspected LAB primarily. The pathogenicity test was conducted by inoculating LAB on blood agar medium. Furthermore, biochemical tests are carried out using the KB020 kit. The results showed that the highest population of LAB in the small intestine (1.57 × 108 CFU/mL) was observed with 2% FPH supplementation. In comparison, the caecum yielded the highest population (1.22 × 108 CFU/mL) under 1.5% FPH. Giving 2% FPH  to broiler chicken feed provides a weight gain of 1.021 kg/head. The primary characteristics of the eight bacterial colony isolates suspected of being LAB were Gram-positive, catalase-negative, and did not form endospores. Eight LAB isolates of probiotic candidates were non-pathogenic as indicated by the occurrence of α-hemolysis and γ-hemolysis. Biochemical identification of probiotic candidates yielded four types of Lactobacillus, namely L. mucosae, L. frumenti, L. sanfranciscensis, and L. ferintoshensis. These LAB strains show promising probiotic potential for use as a feed additive in the broiler production system.
Mosquitoes' larval habitat characteristics and Aedes aegypti resistance status to malathion in Jember Nihayah, Husnatun; Purwatiningsih, Purwatiningsih; Utami, Eva Tyas; Fajariyah, Susantin; Lelono, Asmoro; Puspitasari, Reni; Rohmah, Hidayatur
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 02 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i02.1037

Abstract

Water-holding containers serve as breeding sites for Aedes aegypti. The control of mosquito breeding sites and the use of household insecticides are some of the DHF control methods. The use of household insecticides over a long period can reduce the susceptibility of mosquitoes to these insecticides. This research aims to determine the mosquito's habitat characteristics and to determine the Aedes aegypti resistance status to malathion in Jember.  A total of 61 houses were surveyed, and the various types of containers encountered at the sampling locations were recorded. Larvae and pupae found at the sampling location are brought and hatched in the laboratory. Aedes aegypti was tested for resistance status to malathion insecticide using a CDC bottle bioassay and biochemical nonspecific esterase enzyme activity. A total of 140 containers were found in this study, and 36.43% of them are Aedes aegypti larvae/pupae habitat. Types of containers used as mosquito larval habitat are bottles/glasses, bathtubs, used basins/buckets, water storage buckets, trash cans, refrigerator water containers, used cans, gutters, livestock drinking water containers, fish ponds, plant pots, used livestock cages, used toilets, used jugs, used animal feeders, and used aquariums. The mortality of Aedes aegypti reached 42.3% (resistant). Meanwhile, the results of biochemical tests showed an increase in the activity of the nonspecific esterase enzyme. This study concludes that Aedes aegypti from Jember has been resistant to malathion insecticide
Struktur Histologi Trabekula Femur Mencit (Mus musculus L) Orkidektomi Pasca Pemberian Ekstrak tempe Kedelai Susantin Fajariyah; Siti Nafi’atul Muta’alimah; Eva Tyas Utami; Asmoro Lelono; Husnatun Nihayah
JURNAL BIOS LOGOS Vol. 14 No. 3 (2024): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v14i3.56668

Abstract

Testosterone deficiency is caused by, among other things, old age, chronic disease, and testicular cancer, which results in decreased bone density. Estrogen plays a more important role in the process of bone growth and resorption than the hormone testosterone. Therefore, to prevent a decrease in density in conditions of testosterone deficiency, it is necessary to administer external phytoestrogens, including tempeh, containing isoflavones deidzein dan genistein. The aim of this study was to determine the effect of administering tempeh extract on the histological structure of the femoral trabeculae of orchidectomized mice. Mice were divided into 4 groups, namely negative control (without orchidectomy), positive control (orchidectomy), and 2 orchidectomy treatment groups, followed by administration of tempe extract at a dose of 0.6 g/ml/day (D1) and 1.2 g/ml/day (D2). Tempeh extract was administered by gavage for 15 days after a 30-day healing period for bilateral orchidectomy. One day after the last administration of tempeh extract, the mice were anesthetized and dissected to remove the femur bones to make preparations using the paraffin method and HE staining. The parameters observed were the number of osteoblasts and osteoclasts and the thickness of the femoral trabeculae. Giving soybean tempeh extract at a dose of 0.6 mg/ml/day by gavage for 15 days can cause an increase in the number of osteoblasts and trabecular thickness, as well as reducing the number of osteoclasts in the femur of orchidectomized mice.
DHF Prevention Efforts in Sumbersari Jember: Assessment of Aedes aegypti Resistance Status to Deltamethrin Reni Puspitasari; Hidayatur Rohmah; Husnatun Nihayah; Purwatiningsih; Eva Tyas Utami; Susantin Fajariyah; Asmoro Lelono
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19883

Abstract

This study aims to determine the resistance status of Ae. aegypti to the deltametrin insecticide in Sumbersari Jember Village. A total of 100 2nd generation female Ae. aegypti were tested using the CDC bottle bioassay with a deltamethrin dose of 10 μg/ml and a diagnostic time of 30 minutes. Molecular detection using AS-PCR was also performed to detect kdr mutations at position 1016 of the VGSC gene. Mortality data was calculated using Ms.Excel with a confidence level of 95%. The molecular wight of the PCR product is calculated using a gel analyzer application.  The results of the CDC bottle bioassay show that the Ae aegypti population is still tolerant to the insecticide deltamethrin (91,25% in mortality rate). However, molecular analysis confirms the presence of a mutation at position 1016 of the voltage-gated sodium channel (VGSC) gene (homozygous mutation), which is known to be the primary target of pyrethroid insecticides. These findings indicate that although phenotypic resistance has not yet fully developed, the mosquito population has shown early indications of target-site insensitivity to deltamethrin, which has the potential to develop into resistance under sustained selection pressure. Further use of deltamethrin insecticide may potentially increase phenotypic resistance, namely a decrease in mosquito mortality after exposure to deltamethrin insecticide. Therefore, an integrated vector control strategy and routine monitoring of mosquito resistance status in the region are required.
Co-Authors Achmad Sjaifullah Agustina, Rizka Ahmad Alfan Abdullah Anggitasari, Dhanti Fatma Anik Suwandari Anik Swandari Aurellia, Naurah Septa Belkis, Malika Bella Dwi Arifianti Dewi Syaibatul Aqlina Dita Ayu Faradila Dwi Setyati Esti Utarti Hidayat Teguh Wiyono Hidayat Teguh Wiyono Hidayatur Rohmah Ika Dewi Kusumaningtyas Indah Salsabila Kurnia Intani, Rini Nur Kurnia, Indah Salsabila Lelono, Asmoro Luh Putu Ratna Sundari Lutfia Azizah Mahriani Mahriani Mahriani Mahriani Mahriani Mahriani Masrurotul Hasanah Medayani, Rani Dian Nashrullah, Shafa Nidaul Hikmah Nihayah, Husnatun Nur Asyia Alfiyani Primasari, Nindita Fitria Purniasari, Fina Yunita Purwatiningsih Purwatiningsih Purwatiningsih Purwatiningsih Puspitasari, Reni Rendy Setiawan Reni Puspitasari Resa Miftahatu Yuniar Rike Oktarianti Rohmah, Hidayatur Siti Nafi’atul Muta’alimah Sudarmadji Sudarmadji Susantin Fajariah Susantin Fajariyah Ulfi Risqillah Viqi Ainullah, Muhammad Wardhani, Dinna Wahyu Putri Wijaya, Rizky Surya Wimbaningrum, Retno Wuryanti Handayani Yudha Nurdian Yunita Arisandi