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All Journal HAYATI Journal of Biosciences Jurnal Sain Veteriner Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Kedokteran Hewan Animal Production

Rini Widayanti

Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Author-ID : 415159

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Veterinary

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VARIASI GENETIK KAMBING BENGGALA DI KABUPATEN MANGGARAI BARAT BERDASARKAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA Pakpahan, Suhendra; Artama, Wayan Tunas; Widayanti, Rini; Budisatria, I Gede Suparta
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 2 (2018): December 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Genetic Variation of Benggala Goats in West Manggarai Regency Based on Random Amplified Polymorphic DNA Method ABSTRACTIndonesia has several types of local goats that have had an extended period of adaptation to the natural conditions in Indonesia. Goat is one of the most important germplasm in supporting the economy of rural communities. Benggala is a local breed of goat originating from Flores Island, East Nusa Tenggara province and has distinctive characteristics. The RAPD technique has several advantages and has been widely used in studies of the genetic diversity of goats. A total of 50 blood samples of Benggala goats were taken from four sub-districts in West Manggarai Regency. This study was conducted to estimate genetic variations of Benggala goats using OPA-6 and OPA-16 primers. The OPA-6 primer consisted of 0-11 bands, while the OPA-16 primer consisted of 0-7 bands. The total bands produced on the OPA-6 primer from all samples was 456, whilst OPA-16 primer was 314. The lowest genetic similarity between individuals of Benggala goats was 44% from the sample K46. Based on the sample population, the average genetic similarity level was 72%. These results show that the genetic diversity of Benggala goats is low.Keywords: Benggala goat, genetic similarity,genetic variation, RAPD, West Manggarai ABSTRAKIndonesia memiliki beberapa jenis kambing lokal yang memiliki periode adaptasi yang panjang dengan kondisi alam di Indonesia. Kambing merupakan salah satu plasma nutfah yang sangat penting dalam mendukung perekonomian masyarakat pedesaan. Benggala adalah jenis kambing lokal yang berasal dari pulau Flores, propinsi Nusa Tenggara Timur dan kambing Benggala memiliki ciri khas. Teknik RAPD memiliki beberapa keunggulan dan telah banyak digunakan pada studi keragaman genetik kambing. Total 50 sampel darah kambing Benggala yang diambil dari empat kecamatan di Kabupaten Manggarai Barat. Penelitian ini dilakukan untuk menguji variasi genetik kambing Benggala dengan menggunakan primer OPA-6 dan OPA-16. Primer OPA-6 terdiri dari 0-11 band, sedangkan primer OPA-16 terdiri dari 0-7 band. Total jumlah pita yang dihasilkan pada primer OPA-6 dari semua sampel adalah 456, sementara primer OPA-16 adalah 314. Kemiripan genetik terendah antara individu-individu kambing Benggala adalah 44% dari sampel K46. Berdasarkan populasi sampel, tingkat kemiripan genetik rata-rata adalah 72%. Hasil ini menunjukkan bahwa keragaman genetik kambing Benggala tergolong rendah.Kata Kunci: kambing Benggala, kemiripan genetik, Manggarai Barat , RAPD, variasi genetik

Kajian Penanda Genetik Tarsius bancanus dan Tarsius spectrum dengan Sekuen D-Loop Parsial DNA Mitokondria Widayanti, Rini; Solihin, Dedi Duryadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 3 (2007): October 2007
Publisher : Universitas Atma Jaya Yogyakarta

The objective of this research was to study the specific genetic marker on D-loop region of Tarsius bancanus and Tarsius spectrum. The sequencing of PCR product using primer DLTARPROF on D-loop resulted in base sequence of 270 nts. Result of D-loop fragments sequencing was put on multiple alignment with other primates from Genbank with the aid of software Genetyc-Win Version 3.0 and Clustal W, and was analyzed using MEGA program version 3.1. The genetic distance was based on nucleotide D-loop, the smallest genetic distance was 0% and the biggest was 11.8% and the average was 2.3%. The phylogenetic tree using neighbor Joining Method based on some nucleotide sequence on D-loop region could not be used to differentiate between Tarsius bancanus and Tarsius spectrum.

Kajian Molekular Tarsius sp. Pada Gen Penyandi Cytochrome Oxidase Sub-unit 2 Mitokondria Widayanti, Rini; Handayani, Niken Satuti; Budiarsa, I. Made
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Tarsius is an endemic species in Indonesia that is endangered. In-situ and ex-situ conservation of this species would yield better results if this genetic make up and diversity is determined. The objective of this ressearch was to study the specific genetic marker on COX2 gene of Tarsius sp. Sequencing of PCR product using primer COX2F and COX2R resulted in base sequence of 513 nts. Results of COX2 fragments sequencing were put on multiple alignment with other primates from Genbank with aid of software Clustal W, and were analyzed using MEGA program version 4.1. Eight different amino acid sites were found (amino acid no. 5, 6, 13, 14, 30, 35, 44 and 168). The genetic distance based on nucleotide COX2 calculated using Kimura 2-parameter model indicated that in the smallest genetic distance 0%, biggest 6.8% and average 2.3%. The phylogenetic tree using neighbor joining method based on the sequence of nucleotide and amino acid COX2 reveded differentiation among Tarsius from Lampung and Tarsius from Sulawesi, but could not be used to differentiate among T. dianae (from Central Sulawesi) and T. spectrum (from North Sulawesi).

Frekuensi Nafas dan Pulsus Ular Sanca Batik (Malayopython reticulatus) Lokalitas Jawa Raharjo, Slamet; Hartati, Sri; Indarjulianto, Soedarmanto; Widayanti, Rini
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Reticulated python (Malayopython reticulatus) java locality is highly demand as pet animal. Respiratory and pulse rate were important physiological data needed for diagnostic references. The aim of this research was to study the repiratory and pulse rate on Java M. reticulatus. Thirthy healthy adult Java M. reticulatus were used in this study. The respiratory rate recorded by counting inspiratory and expiratory movement of the thorax for one minute and pulse rate recorded by heart palpation for one minute, measured at 6-8 AM and at 4-6 PM for five consecutive days. Temperature and humidity were record as secondary data. The data were analyzed using independent t-Test. The respiratory rate in this study recorded in the morning ranged 5-48 breaths/minute averaging 20 breaths/minute and in the evening ranged 7-49 breaths/minute averaging 24 breaths/minute. The pulse rate in the morning ranged 21-68 beats per minute (bpm) averaging 45.5 bpm and in the evening ranged 40-82 bpm averaging 56 bpm. Totally, M. reticulatus having the respiratory rate 5-49 breaths/minute and pulse rate 21-82 bpm. The morning temperature ranged 25-27oC and the evening 26-27.5oC. The range of morning and evening air humidity is 66-75% and 65-70% respectively. The results showed that the comparison of respiratory rate (R) and pulse rate (P) were in normal range namely 1:2.25 in the morning and 1:2.33 in the evening. There were no significant differences (P>0.05) between morning and evening respiratory rate and no significant effect (P>0.05) of temperature and humidity on respiratory and pulse rate. It could be concluded that healthy adult Java M. reticulatus has a respiratory rate 5-49 breaths/minute dan pulse rate 21-82 bpm.

VARIASI GENETIK KAMBING BENGGALA DI KABUPATEN MANGGARAI BARAT BERDASARKAN METODE RANDOM AMPLIFIED POLYMORPHIC DNA Pakpahan, Suhendra; Artama, Wayan Tunas; Widayanti, Rini; Budisatria, I Gede Suparta
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Genetic Variation of Benggala Goats in West Manggarai Regency Based on Random Amplified Polymorphic DNA MethodÃ‚Â ABSTRACTIndonesia has several types of local goats that have had an extended period of adaptation to the natural conditions in Indonesia. Goat is one of the most important germplasm in supporting the economy of rural communities. Benggala is a local breed of goat originating from Flores Island, East Nusa Tenggara province and has distinctive characteristics. The RAPD technique has several advantages and has been widely used in studies of the genetic diversity of goats. A total of 50 blood samples of Benggala goats were taken from four sub-districts in West Manggarai Regency. This study was conducted to estimate genetic variations of Benggala goats using OPA-6 and OPA-16 primers. The OPA-6 primer consisted of 0-11 bands, while the OPA-16 primer consisted of 0-7 bands. The total bands produced on the OPA-6 primer from all samples was 456, whilst OPA-16 primer was 314. The lowest genetic similarity between individuals of Benggala goats was 44% from the sample K46. Based on the sample population, the average genetic similarity level was 72%. These results show that the genetic diversity of Benggala goats is low.Keywords: BenggalaÃ‚Â goat, genetic similarity,genetic variation, RAPD, West ManggaraiÃ‚Â ABSTRAKIndonesia memiliki beberapa jenis kambing lokal yang memiliki periode adaptasi yang panjang dengan kondisi alam di Indonesia. Kambing merupakan salah satu plasma nutfah yang sangat penting dalam mendukung perekonomian masyarakat pedesaan. Benggala adalah jenis kambing lokal yang berasal dari pulau Flores, propinsi Nusa Tenggara Timur dan kambing Benggala memiliki ciri khas. Teknik RAPD memiliki beberapa keunggulan dan telah banyak digunakan pada studi keragaman genetik kambing. Total 50 sampel darah kambing Benggala yang diambil dari empat kecamatan di Kabupaten Manggarai Barat. Penelitian ini dilakukan untuk menguji variasi genetik kambing Benggala dengan menggunakan primer OPA-6 dan OPA-16. Primer OPA-6 terdiri dari 0-11 band, sedangkan primer OPA-16 terdiri dari 0-7 band. Total jumlah pita yang dihasilkan pada primer OPA-6 dari semua sampel adalah 456, sementara primer OPA-16 adalah 314. Kemiripan genetik terendah antara individu-individu kambing Benggala adalah 44% dari sampel K46. Berdasarkan populasi sampel, tingkat kemiripan genetik rata-rata adalah 72%. Hasil ini menunjukkan bahwa keragaman genetik kambing Benggala tergolong rendah.Kata Kunci: kambing Benggala, kemiripan genetik, Manggarai Barat , RAPD, variasi genetik

Klasterisasi Staphylococcus aureus Resisten Neutrofil Berdasar Assesory Gene Regulator Santosa, Christin Marganingsih; Lestari, Fajar Budi; Widayanti, Rini; Salasia, Siti Isrina Oktavia
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.50653

Staphylococcus aureus is recognized worldwide as a major pathogen causing subclinical intramammary infections in dairy cows and food poisoning due to its ability to produce enterotoxin. The study aimed to identify enterotoxins of S. aureus and clustering the enterotoxins based on assessory gene regulator (agr). Virulence of S. aureus to the host was characterized based on the response of polymorphonuclear cells to the infection. Twelve S. aureus could be isolated from milk cows in central of dairy farming in Sumedang West Java. The identification of S. aureus was based on cultural and biochemical tests and an amplification of a specific section of the 23S rRNA gene. The sensitivity test against antibiotics revealed that some isolates of S. aureus were resistant to penicillin and methycillin. By PCR amplification one or more staphylococcal enterotoxin genes could be observed five genes in combinations of sea (216 bp), seb (478 bp), seh (375 bp), sei (576 bp), and sej (142 bp). Clustering of S. aureus based on the assesory gene regulator could be grouped into 4 clusters for agr1 (1 isolat), agr2 (2 isolates), in combination for agr1 and agr2 (1 isolate), and for non agr (2 isolates). Based on the response of polymorphonuclear cell in vitro and in vivo assays, revealed that S. aureus strain I-2 (agr1 cluster) and P1 (agr1+agr2 cluster) were more resistant to polymorphonuclear cells and could survive intracellularly, indicated that these strains could be used as proper candidates to develop dignostic tool based on agr against staphylococcal mastitis.

Spike Glycoprotein 1 Partial Gene Analysis of GI-19 (QX-like) Infectious Bronchitis Virus Isolated and Propagated from Breeder, Broiler, and Layer Chickens in Java Region Santoso, Ferdinand Prayogo Cahyo; Widayanti, Rini; Widyarini, Sitarina; Wibowo, Michael Haryadi
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.414-424

This study aims to identify and characterize receptor binding sites (RBS) and antigenic sites (HVR-I and II) of the S1 gene fragment of the infectious bronchitis virus (IBV) isolated and propagated from commercial chickens in Java Region to monitor recent circulating virus. The samples in this study were the organs which indicated infectious bronchitis infection. The stages of this research consisted of making virus suspensions, isolation, and propagation, as well as molecular detection and characterization of viruses. Virus isolation and propagation were carried out on chicken embryonated eggs aged 11 days via the allantoic route. Culture confirmation was performed by RT-PCR of the S1 gene fragment, followed by sequencing and bioinformatics analysis. The 168-hour propagation was observed in both dwarfed and curled embryos of two isolates from 11 isolates detected as IBV-positive. Phylogenetic tree construction resulted in all isolates being grouped as GI-19 genotype (QX-like). Amino acid identity among QX-like strains was calculated at 87–100%. A total of 210 predicted amino acid residues were observed, including 31 substitutions and 2 deletions. Conclusions of this study were identified and characterized as GI-19 genotype (QX-like) IBV with amino acid changes on S1 fragment from breeder, broiler, and layer chickens in Java Region.

Distinct Island Lineages of Binturong (Arctictis binturong) from Indonesia and Its Conservation Implications Hardian, Andreas Bandang; Rahmawati, Irhamna Putri; Widayanti, Rini; Aninta, Sabhrina Gita
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.916-928

Binturong (Arctictis binturong) is a threatened carnivore that inhabits the forests of South and Southeast Asia. Despite its wide range, binturong is relatively scarce across its habitat distribution and is currently under the threat of poaching and illegal trade. Captive breeding has unfortunately been conducted rather haphazardly with a lack of origin record maintained, implicating potential risks to the management such as inbreeding or genetic swamping. This study thus aims to characterise the phylogenetic relationship of Indonesian binturong within the context of Southeast Asian binturong and further probe the distinctness of lineages originating from Java, Sumatra, Indonesian Borneo, and Bangka using Cytochrome B (CytB) and Cytochrome C Oxidase Subunit 1 (CO1). Genetic distance, phylogram topology, and haplotype analysis of both encoding genes further corroborate the distinctness of Java, Borneo, and Bangka binturong from other binturong from Indochinese regions such as India, Laos, and Myanmar. Search for prospective single nucleotide polymorphism markers to discriminate island lineages consistently found that each Java, Bangka, and Bornean binturong be distinct from each other and other lineages, especially when assessed using haplotype-based clustering. Assigning binturong originated from Sumatra is nonetheless more complicated, suggesting the possibility. Our findings substantiated the much-needed systematic research of Southeast Asian binturong as ex-situ insurance population management grows in Indonesia and other parts of the world to protect the diversity of binturong lineages and their corresponding unique evolutionary history.

Sacrificers' Preferences on Selection and Procurement of Sacrificial Animals for Eid al-Adha Celebration Ibrahim, Alek; Budisatria, I Gede Suparta; Artama, Wayan Tunas; Widayanti, Rini; Atmoko, Bayu Andri
ANIMAL PRODUCTION Vol. 24 No. 1 (2022)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2022.24.1.99

Eid al-Adha is one of the biggest religious celebrations in Indonesia. This study was conducted to determine the profile and activities of sacrificers (shohibul qurban) in selecting and procuring sacrificial animals for the Eid al-Adha celebration. This study was conducted by in-depth interviews with a total of 54 sacrificers in Sleman Regency, Yogyakarta, during the Eid al-Adha celebration in 2019. The data were analyzed using descriptive analysis (number and percentage). This study indicated that most sacrificers were classified as productive age (45.35 years), high school graduates (46.30%), and self-employed workers (50.00%) with an income of IDR 3,357,896 per month. The sacrificial animals were cattle, sheep, and goats with an average body weight of 253.75 kg, 27.80 kg, and 32.80 kg, and the prices of IDR 20,939,962; IDR 2,906,250; and IDR 2,420,000, respectively. The sacrificial animals were mainly purchased from the livestock seller or intermediary seller (38.89%). There were livestock-price differences in the different marketplaces (44.44%) and between Eid al-Adha and the daily period (61.11%). This study concluded that the cattle preferred animal types for group sacrifices, and the sheep had preferred animal types for individual sacrifice. Cattle are usually sacrificed with a joint purchase between sacrificers. Each reason for selecting and procuring the sacrificial animal types was influenced by different motivations, such as financial, animal handling, and local wisdom factors.

Isolasi dan Identifikasi Methicillin Resistant Staphylococcus aureus dan Methicillin Resistant Staphylococcus pseudintermedius pada Kasus Klinis Anjing dan Kucing di Yogyakarta Alhadz, Ghias Ghifari; Salasia, Siti Isrina Oktavia; Yosyana, Alyaa Rifqoh Putri; Wasissa, Madarina; Lestari, Fajar Budi; Widayanti, Rini
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.100240

Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP), are known as bacterial resistant to various antibiotics. MRSA transmission occurs between humans and direct human contact with dogs and cats. This study aimed to isolate, characterize, and analyze bacterial resistance to antibiotics phenotypically and genotypically from clinical case samples of pet animals in Yogyakarta. Bacterial isolates were identified through biochemical tests and molecular identification by species-specific 23S rRNA and nuc genes with polymerase chain reaction (PCR) and pta genes with PCR-restriction fragment length polymorphism (RFLP). Bacterial resistance to several antibiotics was analyzed using disk diffusion Kierby-Bauer method. The occurrence of methicillin resistance were screened by oxacillin resistance screening agar base (ORSAB) and confirmed by detection of the mecA gene with PCR. Based on the phenotypic and genotypic identification showed that 11 samples were identified as S. aureus (6 isolates) and S. pseudintermedius (5 isolates). The resistance tests revealed that 36% were resistant to tetracycline, cefoxitin (27%), oxacillin and erythromycin (9%). Screening of methicillin resistance with ORSAB indicated that all isolates were methicillin resistances (100%). Detecting the mecA gene encoding methicillin resistance showed that 81.81% of isolates were resistant to methicillin, with detail S. aureus (all 6 isolates) and S. pseudintermedius (4 isolates). Based on the antibiogram, ORSAB, and mecA gene detection, it could be confirmed that three isolates were MRSP, and six isolates were MRSA. The results of this study indicate the high occurrence of methicillin-resistant strains in pets that have the potential zoonotic spread to other animals and humans.

Co-Authors Alek Ibrahim Alhadz, Ghias Ghifari Alnita Baaka Aninta, Sabhrina Gita Ariana Ariana Aris Haryanto Aris Purwantoro Atmoko, Bayu Andri Christin Marganingsih Santosa Claude Mona Airin Fajar Budi Lestari Handayani, Niken Satuti Hardian, Andreas Bandang Herrialfian H Hery Wijayanto I Gede Suparta Budisatria Jalaluddin J Laksono Trisnantoro Michael Haryadi Wibowo Pakpahan, Suhendra Pakpahan, Suhendra Rahmawati, Irhamna Putri Santoso, Ferdinand Prayogo Cahyo Sari, Yuslikha Khaniif Anggita Sarmin Sarmin Sitarina Widyarini Siti Isrina Oktavia Salasia Slamet Raharjo Soedarmanto Indarjulianto Solihin, Dedi Duryadi Sri Hartati Teuku Zahrial Helmi Teuku Zahrial Helmy Tri Wahyu Pangestiningsih Trini Susmiati Tunas Artama Wasissa, Madarina Wayan Tunas Artama Yosyana, Alyaa Rifqoh Putri Yuda Heru Fibrianto Yuriadi Y

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