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LEYDIG CELL COUNT IS INCREASES IN OLD WISTAR RATS (RATTUS NORVEGICUS) BY ANTIOXIDANT ETHANOL EXTRACT OF MORINGA OLEIFERA (EEMO) Widiastini, Luh Putu; Karuniadi, I Gusti Agung Manik
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 1 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5807

Abstract

Oxidative stress is the result of an imbalance between antioxidant production and the production of reactive oxygen species (ROS). Leydig cells have a high content of PUFAs in their cell membrane, making it easy to bind ROS. Most natural antioxidants are found in plants, including Moringa. In this work, aged Wistar rats' Leydig cells will be used to test the antioxidant activity of an ethanol extract from the leaves of Moringa oleivera (Rattus norvegicus). The study involved the division of 36 elderly rats, who were between the ages of 18 and 19 months, into two groups. For thirty days, the control group received the same quantity of 0.5% CMC every day, whereas the treatment group received 50 mg/kgBW/0.5 mL of Moringa leaf ethanol extract daily. The rats were healthy and free of physical impairments. The Independent Samples T-Test is used to assess the data in order to find any variations between the treatment and control groups. The significant difference (p < 0.00) in Leydig cell counts between the extract-treated group and the control group suggests that the ethanol extract of Moringa leaves can have a major effect on the number of Leydig cells in Old Wistar rats (Rattus Norvegicus).
PENGARUH SELF HEALING PADA IBU BERSALIN KALA I FASE AKTIF TERHADAP INTENSITAS NYERI PERSALINAN Supawitri, Ni Made; Purnamayanthi, Pande Putu Indah; Karuniadi, I Gusti Agung Manik; Widiastini, Luh Putu
Jurnal Kesehatan Tambusai Vol. 6 No. 4 (2025): DESEMBER 2025
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v6i4.48067

Abstract

Persalinan merupakan suatu proses lahirnya bayi dan plasenta dari rahim ibu dengan umur kehamilan 38 minggu sampai dengan 42 minggu. Persalinan merupakan perjalanan alamiah yang nantinya akan dihadapi oleh setiap ibu hamil yang akan bersalin. Kondisi psikologis juga menjadi salah satu faktor yang mempengaruhi lamanya kemajuan persalinan. Kondisi psikologis yang dimaksud yaitu persepsi ibu terhadap cemas dan rasa nyeri saat menjalani proses persalinan. Rasa nyeri saat bersalin akan memberikan respon fisiologis pada ibu yang menyebabkan berkurangnya kemampuan rahim dalam keadaan kontraksi dan hal tersebut berefek terhadap panjangnya waktu persalinan. Usaha yang dapat dilakukan untuk menurunkan rasa nyeri pada berlangsungnya proses persalinan yaitu Self-Healing. Self healing adalah salah satu metode yang lumayan mendapatkan perhatian karena banyak yang beranggapan bahwa self-healing bisa membantu ibu dalam mengendalikan amarah, emosi dan kecemasan saat berlangsungnya proses persalinan. Tujuan penelitian ini untuk mengetahui Pengaruh Self Healing Pada Ibu Bersalin kala I Fase Aktif Terhadap Intensitas Nyeri Persalinan Di Ruang Bersalin RSD Mangusada. Desain penelitian adalah quasi experiment dengan rancangan nonequivalent control group. Sampel dalam penelitian ini yaitu ibu bersalin kala I fase aktif yang mengalami nyeri persalinan sebanyak 40 responden. Pengambilan sampel menggunakan teknik accidental sampling. Hasil analisis menggunakan uji mann-whitney pada kelompok intervensi dan kelompok kontrol didapatkan nilai nilai p-value sebesar 0,001 yang artinya terdapat pengaruh self healing terhadap nyeri persalinan di Ruang Bersalin RSD Mangusada. Diharapkan Self Healing dapat dilakukan sebagai salah satu metode untuk mengurangi intensitas nyeri persalinan kala I fase aktif.
Pengaruh Senam Lansia Terhadap Hot flashes Pada Wanita Menopause Karuniadevi, Gusti Ayu Made Wid; Lestari, Ni Putu Yunita Sri; Widiastini, Luh Putu; Susila, I Made Dwie Pradnya
Jurnal Ilmiah Kesehatan Mandala Waluya Vol. 5 No. 2 (2025): Jurnal Ilmiah Kesehatan Mandala Waluya
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54883/jikmw.v5i2.1126

Abstract

Menopause adalah berakhirnya siklus menstruasi secara alami, biasanya terjadi saat wanita memasuki usia 40-58 tahun  yang menimbulkan gejala fisik salah satunya adalah hot flashes. Kondisi menopause disertai hot flashes bisa menimbulkan ketidaknyamanan dan bila tidak ditangani segera maka akan berdampak buruk bagi kesehatan yaitu dapat meningkatkan resiko penyakit jantung, osteoporosis, demensia, dan stroke. Upaya yang dilakukan untuk penanganan hot flashes secara non farmakologi yaitu senam lansia. Tujuan penelitian ini adalah untuk mengetahui pengaruh senam lansia terhadap hot flashes pada wanita menopause di Banjar Celuk, Desa Adat Kapal. Metode penelitian ini menggunakan metode pra eksperimental dengan desain one group pretest. Teknik pengambilan sampling yang digunakan adalah total sampling dengan jumlah sampel adalah 35 responden. Intervensi senam lansia diberikan 2 kali seminggu selama 3 minggu dengan durasi 30 menit. Uji analisis data menggunakan uji Wilcoxon untuk membandingkan hasil sebelum dan sesudah diberikan intervensi. Hasil penelitian didapatkan p-value = 0,001 < α 0,05 yang artinya ada pengaruh senam lansia terhadap hot flashes pada wanita menopause di Banjar Celuk, Desa Adat Kapal. Pentingnya meningkatkan pemahaman masyarakat mengenai senam lansia yang mudah diterapkan di rumah untuk membantu mengatasi hot flashes tanpa ketergantungan pada obat-obatan sehingga dapat memiliki pilihan yang lebih alami dan terjangkau untuk mengatasi hot flashes
The Effectiveness of Administering Pure Honey on Increasing Hemoglobin in Adolescent Girls in Sibangkaja Village Ni Made Surya Dewi; Luh Putu Widiastini; Putu Ayu Dina Saraswati
Jurnal Ilmiah Kebidanan (The Journal Of Midwifery) Vol. 12 No. 1 (2024)
Publisher : Midwifery Department, Poltekkes Kemenkes Denpasar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jik.v12i1.3206

Abstract

Anemia defined as a condition in when the count of body's red blood cell’s lower than normal. Teenagers can overcome anemia naturally, by drinking honey. This study aimed to investigate the effectiveness of administering pure honey to improve hemoglobin in adolescent. This study was quasi-experimental design with pretest and post-test control group. Samples was 36 respondents (18 respondents for each control group and treatment group) which determined by total sampling technique. The research was conducted in November-December 2023. The data collection technique included pretest and post-test questionnaires for both groups, Haemoglobin (Hb) sticks was used to measure the Hb levels. Data analysis included descriptive analysis, and comparison analysis using the Wilcoxon and Mann Whitney tests. The study's findings revealed that before the intervention, both groups had anemia. The study yielded a z- value of -3.429 and p-value of 0.001 (p<0.05). Administering pure honey to young women had been shown increase hemoglobin levels. The findings will be utilized to propose midwifery services as a non-pharmacological method of reducing anemia in teenagers, particularly young women.
The Impact of the Consumption of Moringa Leaf on the Production of Postpartum Breast Milk in Puskesmas III Denpasar Selatan Working Area Mothers Ade Dwidya Pradnyani; Luh Putu Widiastini; Ni Putu Yunita Sri Lestari
Jurnal Ilmiah Kebidanan (The Journal Of Midwifery) Vol. 12 No. 1 (2024)
Publisher : Midwifery Department, Poltekkes Kemenkes Denpasar, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jik.v12i1.3258

Abstract

A dietary item that is high in nutrients is moringa (moringa oleifera). Many phytosterols found in moringa leaves can help boost the production capacity of breast milk. The motivation behind the research was to find out how eating moringa leaves affected the quantity of breast milk produced by new moms in the Puskesmas III Denpasar Selatan Working Area. The investigation's design involves a control group plus a pre-post test, utilizing a quasi-experimental methodology. 40 people made up the sample, which was selected by random sampling. using the Mann-Whitney and Wilcoxon tests to analyze descriptive and comparative data. After the intervention, the average research results were 1.2 (fluent) with a p-value of 0.001 < 0.05, showing that the consumption of the Moringa leaf intervention altered the average amount of breast milk produced by the control group. Prior to the intervention, the average research results were 1.85 (not fluent). The mean in the control group did not change before or after giving one glass of water prior to breastfeeding; the mean was 1.80 (incoherent) before the intervention and 1.65 (incoherent) after it. This is supported by the p-value of 0.083 > 0.05. Conclusion: In the Puskesmas III Denpasar Selatan work region, postpartum women's production of breast milk is impacted by their use of moringa leaves. Moringa leaves can help postpartum moms whose breast milk isn't flowing easily produce more of it.
Empowering Elementary School Children Through Yoga Pranayama Exercises: An Effort to Improve Emotion Regulation Luh Putu Widiastini; I Gusti Agung Manik Karuniadi; Egar Adhiestiani, Ni Made
Journal of Community Engagement in Health Vol. 9 No. 1 (2026): March
Publisher : Universitas STRADA Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Adolescence is a transitional period between childhood and adulthood, marked by significant biological, psychological, and socio-cultural changes. One of the common issues during this stage is the inability to manage emotions effectively, which can increase the risk of mental disorders such as depression. Interviews with the principal and homeroom teacher at SDN 5 Dalung revealed that fourth- and fifth-grade students. engage in fights or bullying almost every day, reflecting low emotional regulation skills. One intervention that can be implemented is complementary therapy in the form of pranayama yoga. Pranayama yoga is a breathing technique that stimulates the release of endorphins, provides a calming effect, lowers blood pressure, and improves emotional stability. This community service activity was carried out on April 10-11, 2025, and involved 50 fourth- and fifth-grade students. Evaluation was conducted using the DERS-SF instrument (18 items) before and after the intervention. The results showed that before the intervention, 96% of students were in the high emotional regulation difficulty category. After the intervention, this percentage decreased to 66%, with 34% moving into the moderate category. The average DERS score also dropped significantly from 75.08 to 64.72, with a t-test result of t = 6.698 and p = 0.001, which is statistically significant. In conclusion, pranayama yoga has been proven effective in improving emotional regulation among elementary school students and can be used as a simple, safe, and applicable complementary approach to support mental health in schools.
Co-Authors Abrianthi, Ayu Kartika Ade Dwidya Pradnyani Adhiestiani, Ni Made Egar Aenti, Erlyn Falery Agung Manik Karuniadi, I Gusti Andasari, Ni Putu Ayu Desi Anggreni, Ni Putu Ayu Dian Arianty Duany Sinaga Aryati, I Gusti Ayu Widya Astriani, Ni Made Aswitami, Ni Gusti Ayu Pramita Aswitami, Ni Gusti Ayu Pramitha Ayu Dina Saraswati , Putu Desak Made Rusmini Desak Made Widyanthari Dewi, Luh Gede Susila Dewi, Ni Putu Novi Kartika Egar Adhiestiani, Ni Made Ekajayanthi, Pande Putu Novi Ekajayanti, Pande Putu Novi Endelawati, Komang Adi Ervinawati, Ni Putu Pande Gede Susila Dewi, Luh Handayanti, Ida Ayu Gd Sri I Gede Raga I Gede Widhiantara I Gusti Agung Manik Karuniadi I Gusti Agung Manik Karuniadi I Made Jawi I Nyoman Mangku Karmaya I Putu Artha Wijaya I Wayan Rosiana Ida Ayu Diah Julia, A. A. Istri Diah Intan Karuniadevi, Gusti Ayu Made Wid Karuniadi , I Gusti Agung Manik Kristyari, Ayuni Laksono Trisnantoro Lestari, Ni Putu Yunita Sri Made Ririn Sri Wulandari Made Tangkas Made Tangkas Made Tangkas Mahaeni, Made Ayu Maharianingsih, Ni Made Manuaba, I.A Ratih Wulansari Mastiningsih, Putu Maylani Dwi Mirah Yunita Udayani, Ni Putu Mirah, Ida Ayu Ni Luh Putu Dian Yunita Sari Ni Made Egar Adhiestiani Ni Made Egar Adhiestiani Ni Made Risna Ni Made Risna Sumawati Ni Made Risna Sumawati Ni Made Surya Dewi Ni Nyoman Suarti Ni Nyoman Wiantini Ni Putu Eka Wijayanti Ni Putu Eka Wijayanti Ni Putu Ika Santiari Ni Putu Mirah Yunita Udayani Ni Putu Mirah Yunita Udayani Ni Putu Yunita Sri Lestari Ni Wayan Noviani Ni Wayan Purwaningsih Nyoman Kusmaryatni Pande Putu Indah Purnamayanthi Permatasari, Anak Agung Ayu Putri Pramita Aswitami, IGA. Putu Angga Wiradana Putu Ayu Eka Sumariati Putu Dian Yunita Sari, Ni Luh Putu Mastiningsih Reni, Ni Luh Putu Puspa Rusmini, Desak Made Saraswati, Putu Ayu Dina Sri Ariyani Sukma Adnyani Sumawati, Ni Made Risna Sumawati, Ni Made Risna Sumawati Suparinsih, Ni Wayan Dewi Supawitri, Ni Made Surya Darma, I Gusti Gede Mayun Susila, I Made Dwie Pradnya Sutema, Ida Ayu Manik Partha Tangkas , Made Widowati, I Gusti Ayu Rai - Wirasih, Ni Wayan