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Aplikasi Kombinasi Biochar dan Pupuk Hayati pada Tanaman Jagung di Lahan Kering Kabupaten Pandeglang: Application of Biochar and Biofertilizer Combination on Corn in Up Land Pandeglang Regency Dhanti Hanifa Muslimah; Rahayu Widyastuti; Gunawan Djajakirana
Jurnal Ilmu Tanah dan Lingkungan Vol 24 No 2 (2022): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.24.2.47-52

Abstract

Biochar merupakan bahan alternatif pembenah tanah dari pemanfaatan limbah biomassa berpotensi sebagai bagian dari upaya rehabilitasi lahan terdegradasi, khususnya lahan kering. Biochar juga berfungsi sebagai habitat mikrob tanah yang berperan penting dalam ketersediaan unsur hara bagi tanaman. Pemanfaatan pupuk hayati di bidang pertanian merupakan bagian dari dukungan terhadap pertanian berkelanjutan karena bersifat ramah lingkungan. Berdasarkan latar belakang tersebut, penelitian ini bertujuan untuk mengkaji pengaruh aplikasi kombinasi biochar dan pupuk hayati terhadap sifat kimia dan biologi tanah serta pertumbuhan dan hasil tanaman jagung di lahan kering Kabupaten Pandeglang. Percobaan ini merupakan percobaan faktorial dua faktor yang ditempatkan dalam rancangan acak lengkap. Faktor pertama adalah biochar dengan 3 taraf (0; 2.5; 5 ton ha-1) dan faktor kedua adalah kombinasi pupuk hayati cair (PHC) dengan pupuk NPK terdiri atas 5 macam (100% PHC, 100% NPK, 25% PHC + 75% NPK, 50% PHC + 50% NPK, dan 75% PHC + 25%NPK). Masing-masing perlakuan diulang sebanyak 3 kali sehingga terdapat 45 satuan unit percobaan. Hasil penelitian ini menunjukkan aplikasi biochar dosis 5 ton ha-1 (B2) nyata meningkatkan K-tersedia dalam tanah dan tinggi tanaman. Kombinasi pemupukan 75% pupuk hayati cair dan 25% pupuk NPK (P4) nyata meningkatkan jumlah populasi bakteri penambat N2 dan bakteri selulolitik. Kombinasi biochar dosis 2 dengan 100% pupuk NPK (B2P1) merupakan kombinasi perlakuan yang paling baik dalam memperbaiki status unsur hara tanah. Di sisi lain, kombinasi aplikasi biochar dosis 1 dengan pemupukan pupuk hayati cair dan pupuk NPK konsentrasi 50:50 (B1P3) memiliki peluang untuk mendukung pertumbuhan dan hasil tanaman jagung.
Pengaruh Aplikasi Bioarang dan Zeolit pada Fluks CO2, Populasi Mikrob, dan Aktivitas Enzim Mikrob pada Gambut Safira Eka Aprianti; Rahayu Widyastuti; Heru Bagus Pulunggono; Laksmita Prima Santi
Jurnal Ilmu Pertanian Indonesia Vol. 28 No. 1 (2023): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.28.1.170

Abstract

Oil palm plantation on peatlands is considered to contribute to increasing global warming by releasing CO2 as one of the greenhouse gases that cause global warming. The addition of ameliorants, such as biochar and zeolite, can absorb CO2 from soil respiration and store and filter it in their molecular pores. This research aimed to study the effect of ameliorant application on the CO2 flux, water content, microbial population, and enzyme activities. Ameliorant was applied to peat soil with two factors. The first factor was an ameliorant combination with 6 treatment levels: control (A0); zeolite (A1); biochar (A2); zeolite:biochar 75:25; zeolite:biochar 25:75; zeolite:biochar 50:50, and the second factor was 3 levels dose of treatment (% w/w): 1.5%, 3%, and 4.5%. Peat flux CO2 and water content were measured monthly for four months in the laboratory. Without mixing with biochar (A1), the results showed that zeolite suppressed CO2 flux and suppressed the decrease of water content better than other ameliorant combinations. However, different ameliorant dosage percentages did not show any significant results. Ameliorant application and incubation affected the microbial population and enzyme activities, and the results were varied. Chemical characteristics changed after 4 months of incubation. In conclusion, our results indicate that only zeolite amendment can suppress CO2 flux and decrease water content but did not affect microbial population dynamics and enzyme activities. Keywords: ameliorant, CO2 flux, enzyme activities, lignocellulolytic microbes, peat
Respons Tanaman Sorgum atas Aplikasi Mikoriza Arbuskula pada Gradien Konsentrasi N dan P Devanda Ayu Lidya Permata Putri; Rahayu Widyastuti; Idris Idris; Azra Zahrah Nadhirah Ikhwani; Satya Nugroho; I Made Sudiana; Atit Kanti; Masaru Kobayashi
Jurnal Ilmu Pertanian Indonesia Vol. 28 No. 1 (2023): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.28.1.83

Abstract

Sorghum [Sorghum bicolor (L.) Moench] is a multipurpose food crop that can adapt to climate change. Sorghum productivity can be increased by applying chemical fertilizers and arbuscular mycorrhiza fungi (AMF). This research aimed to assess the effect of AMF application and N and P concentration gradients to support Super 2 and KS sorghum accession growth. The plants were grown in a sterilized zeolite (coarse and fine) and inoculated with Glomus mosseae 40 spores per pot. The experimental design was a randomized complete block design with 40 treatments and three replications. N and P concentration gradient treatments were carried out using a dose level of fertilizer N (urea) and P (TSP) at 12,5%; 50%; 75%; 100%; and 125%. Analysis of variance was carried out using SAS 9.0 software at a level of 5%, and the results were significantly different, followed by DMRT. The application of AMF and urea 50% and TSP 75% in Super 2 accessions and urea 50% and TSP 50% in KS accessions gave the highest plant height and biomass. However, the increase in sorghum growth did not correlate with root colonization and root length, which could be seen that the highest in both accessions were obtained at urea 12,5% and TSP 12,5%. The application of AMF can significantly lower the use of urea and TSP fertilizers by up to 50% compared to a positive control (urea and TSP 100%) and support sorghum growth in both accessions. Keywords: Glomus mosseae, Konawe Selatan accession, Super 2 accession, TSP, urea
Abundance of Culturable Bacteria Isolated from Maize Rhizosphere Soil Using Four Different Culture Media ERNIN HIDAYATI; ARIS TRI WAHYUDI; ANTONIUS SUWANTO; RAHAYU WIDYASTUTI
Microbiology Indonesia Vol. 8 No. 1 (2014): March 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (708.386 KB) | DOI: 10.5454/mi.8.1.5

Abstract

Composition and abundance of culturable bacteria of four soil samples (Ktr50II, D50II, G50II, and A50II) were analyzed. The soil samples were collected from maize rhizosphere that planted in dryland Lombok Island. Each soil sample give different growth performance of maize in greenhouse experiment. This study was to investigate the relation of maize growth performance with culturable bacterial community of their rhizosphere and the effect of culture media on number of bacterial isolates recovery. The rhizosphere bacteria were cultured and isolated on commercial media (SEA) and non commercial modification media (NAln, NAln-SE and NAln- RE). Thirty four strains rhizosphere bacteria were obtained from four maize rhizophere soil samples. D50II is the soil sample that caused the better growth performance to the maize, contrary to Ktr50II. D50II has significantly highest number of culturable bacterial types, while significantly lowest on Ktr50II. In D50II, at least 17 bacterial isolates contributed to better growth performance in maize and have relative abundance of dominant isolate not more than 35.34%. In comparing the rhizosphere bacteria recovered using different culture media, bacteria cultivated on SEA have different growth characteristic compared with bacteria cultivated on NAln, NAln SE and NAln-RE. Six bacterial isolates showed antagonistic ability when grew on SEA but not in all of three media. Compared with commercial media, non commercial modification media can increase total isolates recovery about 70.6%.
APLIKASI INOVASI RISET PENDIDIKAN BIOLOGI UNTUK PEMULIHAN LAHAN BEKAS TAMBANG BATU BARA Risa Rosita; Rahayu Widyastuti; Irdika Mansur
Prosiding Seminar Nasional Biologi Vol. 11 (2023)
Publisher : Prosiding Seminar Nasional Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tujuan dari penelitian ini adalah (1) mengetahui pengaruh pemberian C. etunicatum dan Bacillus sp. terhadap pertumbuhan rumput Bede yang ditanam pada tanah bekas tambang batu bara, (2) mempelajari kemampuan serapan hara (N, P, K) rumput Bede yang ditanam pada tanah bekas tambang batu bara. Percobaan penelitian menggunakan Rancangan Acak Kelompok Faktorial (RAKF). Data diolah menggunakan program SAS 9,0 dan dilakukan analisis sidik ragam dengan uji lanjut DMRT (Duncan Multiple Range Test) pada taraf alpha (α) 5%. Pemberian C. etunicatum dan Bacillus sp. efektif meningkatkan pertumbuhan rumput Bede ketika ditanam pada tanah bekas tambang. Pada tanah overburden perlakuan T3P13 (penambahan 50% NPK + 50% dolomit + C. etunicatum + Bacillus sp.) mampu meningkatkan biomassa kering tanaman 660 kali lipat dibandingkan dengan kontrol (T3P0). Sementara itu pada tanah revegetasi pemberian perlakuan T2P10 (kombinasi dolomit 50% + C. etunicatum) menghasilkan berat basah dan berat kering akar paling besar. Rumput Bede yang diperkaya oleh C. etunicatum dan Bacillus sp. yang ditanam pada tanah bekas tambang serta tanah kebun Jati (jenis pemupukan: P4, P5, P6, P7, P8, P9, P10, P11, P12, P13) memiliki nilai serapan hara N, P, K yang lebih tinggi dibandingkan dengan rumput yang tidak diperkaya (P1, P2, P3) dan kontrol (P0). Rumput Bede yang diperkaya dan ditanam pada tanah revegetasi (T2P10) menunjukkan kemampuan serapan hara P paling tinggi dengan nilai serapan sebesar 1,93 g/tanaman.
Ant Abundance, identification and relation with cocoa pest attacks under several shade trees. Abied Khafidhan; Rahayu Widyastuti; Soetanto Abdoellah
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 37 No 3 (2021)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v37i3.474

Abstract

Ants are soil macrofauna that plays an essential role in preventing pods of cocoa (Theobroma cacao L.) from Helopeltis antonii and Conopomorpha cramerella attack. However, the method of those pests control primarily using pesticides and that will disturbed ant's life. This research aims to determine the abundance and diversity of ants in cocoa plantations with different shade and to determine the correlation between ant abundance and intensity of pest attack from Helopeltis antonii and Conopomorpha cramerella. Ants sample was carried out using pitfall traps and Berlese funnels based on purposive sampling method. The results showed that five subfamilies were found in cocoa plantations with different shade. Subfamily Myrmicinae was the dominant group in all observation sites. The ant abundance has a strong correlation with intensity of pest attack. This was influenced by a role of the ant as natural enemies from Helopeltis antonii and Conopomorpha cramerella.
Diversity and Abundance of Earthworms in Cocoa Plantations with Different Shade Trees Niken Puspita sari; Rahayu Widyastuti; Soetanto Abdoellah
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 37 No 3 (2021)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v37i3.483

Abstract

Earthworm is one of the important macro-fauna in cocoa plantation ecosystem that affects the structure and activity of soil microbes. Earthworms maintain nutrient cycle and affect the growth of plants. Cocoa is a plant that requires shade trees. The presence of shade trees is to increase soil fertility and enrich diversity offlora and fauna. The purpose of this study was to investigate the diversity and abundance of earthworms in cocoa farms with different shade trees, namely paranet, areca palm + leucaena, coconut + leucaena, oil palm + leucaena, and leucaena. The study was carried out in Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute, Jember. Earthworms were picked manually to a depth of 30 cm by purposive sampling method from July to October 2020. The cocoa plants used were mature plants with a spacing of 3 m x 3 m. Based on Shannon and Wiener formula, the results showed that earthworm diversity was low in all treatments. Earthworms until family level were identified. Earthworm families of Glososscolicidae and Megascolocidae were found in this study. The highest abundance of earthworms was found in areca palm + leucaena. Soil organic C and N content and sand fraction were positively correlated with earthworm abundance, while silt fraction and soil density were negatively correlated with earthworm abundance. As micro-climate factor, light intensity was positively correlated with earthworm abundance, while air temperature was negatively correlated with earthworm abundance.
Use of Sphingomonas yunannensis to Improve Soil Drought Stress in Chili Plants Ramadhani Sri Astuti, Andi Febrianti; Rahayu Widyastuti; Yusuf, Sri Malahayati
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 1 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.1.195

Abstract

The availability of water plays an important role in plant growth. However, water availability depends on the climate and irrigation channels; therefore, there is little water available for plants during the dry season. Bacteria found in soil can produce exopolysaccharides to survive under extreme land conditions, namely, during drought conditions. The research objectives were to (1) isolate and select bacteria originating from dry land for use on chili plants and (2) determine the effect of water supply frequency and select soil bacteria on chili plants. The experiment consisted of two stages. (1) Bacterial selection and characteristics, which included exopolysaccharide bacteria selection on specific ATCC No. media. 14; pathogenicity test (hypersensitivity and hemolysis); characterization and biochemical testing including pH, temperature, salinity, oxidation, and catalase; bacterial functional tests (P and K solvents and nitrogen-fixing); and (2) tests of chili plants in the greenhouse. The treatment consisted of two factors: (a) application of selected bacteria, (b) frequency of water application (every 1, 2, 3, and 5 days), and (3) molecular identification of selected bacteria. The results showed that the best growth of chili plants was obtained by treating them with bacteria and watering them every other day. Molecular identification demonstrated that the selected bacteria was Sphingomonas yunannensis, which can grow under environmental conditions affected by drought. Keywords: exopolysaccharides, drought, Sphingomonas yunannensis, chili plants
Exploring Soil Biota and Chemical Dynamics in Palm Oil Cultivation: Insights from Cikabayan, Bogor Adiyaksa, Ivan Khofian; Widyastuti, Rahayu; Putri Wulandari, Dwi; A M Zuhud, Ervizal
Media Konservasi Vol. 29 No. 4 (2024): Media Konservasi Vol 29 No 4 September 2024
Publisher : Department of Forest Resources Conservation and Ecotourism - IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/medkon.29.4.609

Abstract

Soil biological properties sometimes escape attention from environmental studies, especially regarding land use change. Several studies on diversity have been conducted in palm oil. However, most of the studies only focus on certain genera or order and have not yet depicted the micro-ecosystem in the soil. This study aims to analyze the interaction between soil biological properties and soil chemical properties through a correlation study using Randomized Block Design (RBD) to simplify the system in the micro-ecosystem. The result showed that most soil microbe parameters have complex and different interactions with soil chemical properties. Soil biological properties, such as the total microbes (TM) and Azotobacter (Az), significantly correlated toward phosphate and potassium, respectively. Furthermore, mesofauna and macrofauna have a negative and weak correlation with pH and a negative and moderate correlation towards Phosphate (P) content in the soil. This negative and moderate correlation strength happened due to increased soil acidity, leading to a higher chance of H2PO4- interacting with metal ions, resulting in a high risk of toxicity. Soil chemical properties have complex interactions with soil biological properties, and each will affect the others to balance the chemical cycling in the soil. Thus, this study showed the importance of preserving the natural balance of cultivated areas, in this case, palm oil plantations, so that the well-preserved ecosystem will give its benefits.
Growth Response of Bambara Groundnut to the Application of Rhizobium sp. and Pseudomonas sp. in Matriconditioning and Nitrogen-Phosphate Fertilization Umadi, Sarah Sakinah; Ilyas, Satriyas; Widyastuti, Rahayu
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.37-46

Abstract

Bambara groundnut has the potential to be an alternative food source of protein and carbohydrates. However, the quality of the seeds is not guaranteed, which can affect plant growth. The experiment aimed to determine the growth response of Bambara groundnut to the application of nitrogen fixation bacteria (Rhizobium sp.) and phosphate solubilizing bacteria (Pseudomonas sp.) integrated into matriconditioning and nitrogen-phosphate (NP) fertilizer. The experiment was conducted in Situraja-District, Sumedang Regency, West Java, Indonesia from November 2020 to April 2021. The experiment was arranged in a split-plot randomized complete block design replicated three times. The main plot was NP fertilizer level (0, 50, and 100% of recommended dose), and the sub-plot was seed invigoration (untreated, matriconditioning + Rhizobium KPB2 + Pseudomonas BPF9, and matriconditioning + Rhizobium KPB5 + Pseudomonas BPF9). Corncob biochar was used as the carrier in matriconditioning. The seed invigoration treatments using matriconditioning + Pseudomonas BPF9 + Rhizobium either KPB2 or KPB5 isolate significantly improved the plant growth (field emergence, plant height, leaf number, canopy diameter, and leaf chlorophyll content). Furthermore, when these invigoration treatments were accompanied by chicken manure 2 t.ha-1 significantly increased the leaf number without adding NP fertilizer.
Co-Authors . Anandyawati . Giyanto . Giyanto A M Zuhud, Ervizal Abda Abda Abied Khafidhan Adiyaksa, Ivan Khofian AHMAD JUNAEDI Ana Khalisha Anandyawati, . Anas Iswandi Andreas Panjaitan Andreas Panjaitan Ania Cintaresmini Ania Citraresmini Ania Citraresmini Ania Citraresmini Antonius Suwanto Aprilianti, Nabila Apsari Putri Wulandari Aris Tri Wahyudi Atang Sutandi Atit Kanti Aviantara, Dwindrata B. Azra Zahrah Nadhirah Ikhwani Bria, Deseriana Budi Nugroho Budi Nugroho Dadang . DADANG DADANG Daniel Happy Putra Darmawan Darwis Delima Napitupulu Delima Napitupulu Devanda Ayu Lidya Permata Putri Dewi Sekar Pengerteni Dewi, Tirta Kumala Dhanti Hanifa Muslimah Dimas Syahiddin Dwi Andreas Santosa DWI ANDREAS SANTOSA Dwi Wulandari Edy Hartulistiyoso Eka Lupitasari Endang Sulistyorini Endang Sulistyorini Enny Widyati Enok Sumarsih ERNIN HIDAYATI Erwinda Erwinda Evi Ambarsari Fahrizal Hazra Fajri, Ihsanul Fany Juliarti Panjaitan Fitriyani, Indri Hapsari Giyanto Gunawan Djajakirana Hariyadi Heru Bagus Pulunggono I Made Sudiana I Nyoman Sujana Ida Widiyawati Idris Idris Idris, Komaruddin Irawati, Arfi Irdika Mansur Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Chaniago Jekvy Hendra Jekvy Hendra Joko Warino Kartika, Sari Yulia Khairiyah, Yaumil Laksmita Prima Santi MAIPA DIA PATI Masaru Kobayashi Maulinda, Restiana Misbakhul Bait Nabila Dianisa Niken Puspita sari Nofrifaldi Ohiwal, Morgan Priyo Cahyono Putri Wulandari, Dwi Ramadhani Sri Astuti, Andi Febrianti Ratnawati Lantifasari Remila Selvany Risa Rosita Rohani Cinta Badia Ginting Rumella Simarmata Rury Kurniawan Safira Eka Aprianti Sanjaya, Wilhelmus Terang Arga Sari Yulia Kartika Sarjiya Antonius SATRIYAS ILYAS Satya Nugroho Soetanto Abdoellah Sri Sulisawati Suciati, Fuzi Sugeng Santoso Sugiarto, Petrus Sugiyanta , Sukmadewi, Desak Ketut Tristiana Sumarsih, Enok Suryo Wiyono Syaiful Anwar Taufiq Hidayat Tita Puspitasari Titik Tri Wahyuni Umadi, Sarah Sakinah Umi Hidayati Umi Hidayati Utami, Aditya Dyah Widrializa, . Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Winda Ika Susanti Wiwik Eko Widayati Yayuk R. Suhardjono Yayuk Rahayuningsih Suhardjono Yayuk Rahayuningsih Suhardjono Yuni Lisafitri, Yuni Yusuf, Sri Malahayati Zuhud, Evrizal A M