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Study of Root Exudate Organic Acids and Microbial Population in the Rhizosphere of Oil Palm Seedling Anandyawati, .; Sumarsih, Enok; Nugroho, Budi; Widyastuti, Rahayu
JOURNAL OF TROPICAL SOILS Vol. 22 No. 1: January 2017
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2017.v22i1.29-36

Abstract

Mutual interaction between plants and microbes occured in the rhizosphere is expected to increase productivity of crops or soil fertility for agriculture. Plants excrete root exudates to attract microbes, and then microbes obtain habitat and food supply from plants and can fulfill the nutrient requirements through assisted enzymatic activity. The objective of the research was to study the types and amounts of root exudate organic acids, microbial population, and the relationship between root exudate organic acids and microbial population in the rhizosphere of oil palm seedlings. The study was conducted in a greenhouse using a planting medium of sterile quartz sand. The study was conducted using two factorials completely randomized design with three replications. The first factor was oil palm seedling age (control / no oil palm seed, 1, 3, 6, 9 and 12 months-old of oil palm seedlings) and the second factor was the periods of seedling growth (45, 90, 135 and 180 days), so in total there were 72 experimental units. The result of High Pressure Liquid Chromatography (HPLC) analysis revealed that four kinds of organic acids were observed in the rhizosphere of oil palm seedlings, with the highest concentration were: acetic acid (1.66 ppm), citric acid (0.157 ppm), malic acid (2.061 ppm) and oxalic acid (0.675) ppm. The highest total population of microbes, fungi, Azotobacter, phosphate solubilizing bacteria (PSB) and phosphate solubilizing fungi (PSF) were 19.38 × 106 cfu g-1 soil, 3.28 × 104 cfu g-1 soil, 12.09 × 105 cfu g-1 soil, 8.39 × 104 cfu g-1 soil and 1.15 × 104 cfu g-1 soil, respectively. There are positive correlations between root exudate organic acids and total microbes, fungi, Azotobacter, PSB and PSF are.Keywords: microbes, organic acids, rhizosphere, root exudates
The Diversity and Abundance of Springtail (Collembola) on Forests and Smallholder in Jambi Widrializa, .; Widyastuti, Rahayu; Santosa, Dwi Andreas; Djajakirana, Gunawan
JOURNAL OF TROPICAL SOILS Vol. 20 No. 3: September 2015
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2015.v20i3.173-180

Abstract

Harapan forests is the first restoration forest in Indonesia, includes several different ecosystems. Different ecosystems have different characteristic to affect the diversity and abundance of Collembola. In the ecosystem, Collembola have an important role in biocontrol, decomposition, soil nutrient distribution, stimulation of soil microbial activity and as an alternative feed for predators. This study was aimed to investigate the diversity and abundance of Collembola in four ecosystems at Harapan forest, i.e. secondary forest, rubber forest, rubber smallholder and oil palm smallholder. To achieve the objective, soil samples were taken at 48 observation points in the four ecosystem. The soil samples were then extracted by Kempson Extractor. The diversity and abundance of Collembola in four ecosystems were categorized as medium to high. The total of individual and population of Collembola in the secondary and rubber forest ecosystems were likely to be higher than in the rubber and oil palm smallholders. This study had also identified four orders, 14 families and 31 genera of Collembola, where Isotomiella and Proisotoma dominated the genus of Collembola in the four ecosystems. [How to Cite: Widrializa, R Widyastuti, DA Santosa and G Djajakirana. 2015. The Diversity and Abundance of Springtail (Collembola) on Forests and Smallholder in Jambi. J Trop Soils 20: 173-180. Doi: 10.5400/jts.2015.20.3.173] 
Effect Carrier Materials of Bradyrhizobium sp. strain PZS_A08 on Growth of Indigofera zollingeriana Sanjaya, Wilhelmus Terang Arga; Kartika, Sari Yulia; Sukmadewi, Desak Ketut Tristiana; Widyastuti, Rahayu; Anas, Iswandi
JOURNAL OF TROPICAL SOILS Vol. 26 No. 2: May 2021
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2021.v26i2.95-103

Abstract

Sustainable forage production is needed to meet animal feed needs as the livestock industry increases. The purpose of this study is to evaluate the effect of liquid and solid carrier material on the effectiveness and infectivity of Bradyrhizobium sp. strain PZS_A08 on the growth of Indigofera zollingeriana (I. zollingeriana). The two carrier materials used were zeolite (solid) and molasses (liquid). This study used two carrier materials consisting of zeolite (solid) and molasses (liquid). The five treatments given were P0 (50% NPK), P1 (50% NPK+liquid biofertilizer), P2 (50% NPK+sterile liquid biofertilizer), P3 (50% NPK+solid biofertilizer), P4 (50 % NPK+sterile solid biofertilizer), P5 (100% NPK). Observations were made on the plant's height, number of leaves, number of nodules, upperparts and root wet biomass, root and upper parts dry biomass, root length, and microbial population). The use of liquid and solid carrier materials effectively affected the effectiveness and infectivity of Bradyrhizobium sp. strain PZS_A08 on  I. zollingeriana. Inoculants Bradyrhizobium sp. strain PZS_A08 significantly increased plants' growth and reduced the use of 50% NPK fertilizer. Cold storage temperature (5ºC) effectively maintained Bradyrhizobium sp. strain PZS_A08 on liquid and solid carriers, while solid carriers showed better effectiveness at room temperature storage (30oC). Through this research, solid carriers such as zeolite are recommended as carriers for Bradyrhizobium sp. filter PZS_A08.
Karakterisasi dan Viabilitas Bakteri Penambat Nitrogen dan Bakteri Pelarut Fosfat dalam Media Pembawa Biochar Ilyas, Satriyas; Widyastuti, Rahayu; Umadi, Sarah Sakinah
Jurnal Ilmu Tanah dan Lingkungan Vol 25 No 2 (2023): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.25.2.40-45

Abstract

A consortium of nitrogen-fixing bacteria and phosphate solubilizers serves as biological fertilizers to increase the availability of plant nutrients such as nitrogen (N) and phosphorus (P). The experiment aims to study the character of nitrogen-fixing bacteria and phosphate solubilizing bacteria and compare their viability on the biochar (made from husk charcoal and corn cobs) as the carrier media. The experiment involved several tests, such as pathogenicity, nitrogen-fixing ability, phosphate solubilization ability on solid Pikovskaya media, compatibility, and viability. The results showed that KPB4 isolates were pathogenic to animals or humans. Nitrogen-fixing bacteria isolates, namely KBP1, KBP2, and KBP5, had N solubility (ppm) of 54.86, 77.79, and 76.28, respectively, and had NH3 concentrations (mg/L) of 66.61, 94.46, and 92.63, respectively. BPF9 (phosphate solubilizing bacteria isolate) had a phosphate solubilization index of 1.14. Each isolate of nitrogen-fixing bacteria was compatible with BPF9. The results showed that KBP2 and KBP5 each consorted with BPF9 on corncob biochar carriers had higher bacteria populations (15.50 x 107 CFU/g) after four weeks of storage. Keywords: nitrogen-fixing, phosphate solvent, consortium bacteria, biochar
Analisis Korelasi Sifat Biologi, Kimia dan Fisika Tanah pada Berbagai Ketinggian Tempat di Bandung, Jawa Barat Rahayu Widyastuti; Sri Malahayati Yusuf; Apsari Putri Wulandari; Fitriyani, Indri Hapsari
Jurnal Ilmu Tanah dan Lingkungan Vol 25 No 2 (2023): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.25.2.64-70

Abstract

Kabupaten Bandung memiliki ketinggian tempat antara 675 mdpl – 2,100 mdpl dengan suhu udara berkisar 14oC sampai dengan 30oC. Perbedaan ketinggian tempat dapat menimbulkan perbedaan iklim dan cuaca sehingga dapat mempengaruhi transformasi hara dan populasi mikrob di dalam tanah. Penelitian ini bertujuan untuk mengetahui korelasi sifat biologi dan kimia tanah, serta kaitannya antara sifat kimia dan fisika tanah pada berbagai ketinggian tempat di Bandung, Jawa barat. Pengambilan sampel tanah menggunakan metode Purposive Random Sampling dengan 5 kali ulangan. Sampel tanah terganggu diambil pada kedalaman 0-20 cm sebanyak ±1 kg, sedangkan contoh tanah utuh diambil menggunakan ring sampler. Pengambilan sampel tanah meliputi empat ketinggian tempat yaitu ketinggian 600 mdpl penggunaan lahan tanaman kol, ketinggian 1000 dan 1200 mdpl penggunaan lahan tanaman selada, serta ketinggian 1400 mdpl penggunaan lahan perkebunan kopi. Hasil analisis menunjukkan bahwa pada ketinggian 1000 mdpl populasi bakteri pelarut fosfat (BPF) dapat tumbuh optimum sebesar 93.6 x 103 CFU g-1 dengan pH tanah agak masam, P-Total sangat tinggi, dan P-Tersedia sedang. Populasi fungi dapat tumbuh optimum sampai ketinggian 1200 mdpl. Total populasi fungi berkorelasi tinggi dengan unsur hara P-Total dan N-Total di dalam tanah. Respirasi tanah berkorelasi positif dengan semua parameter yang berhubungan dengan sifat kimia tanah. Nilai bobot isi terendah pada ketinggian 1200 mdpl bersesuaian dengan nilai C-organik dan porositas tertinggi pada ketinggian tersebut.
Peran Bakteri Penambat Nitrogen untuk Mengurangi Dosis Pupuk Nitrogen Anorganik pada Padi Sawah Widiyawati, Ida; ,, Sugiyanta; Junaedi, Ahmad; Widyastuti, Rahayu
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 2 (2014): Jurnal agronomi indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.112 KB) | DOI: 10.24831/jai.v42i2.8424

Abstract

ABSTRACTThe availability of nitrogen in soil is one of the limiting factors to support growth and rice productivity. Nitrogen-fixing bacteria have ability to utilize air nitrogen so it becomes available in the soil. The use of nitrogen-fixing bacteria could potentially reduce application of nitrogen fertilizer. The aim of the experiment was to determine the role of nitrogen-fixing bacteria in reducing inorganic N fertilizer on lowland rice. The research was conducted in April-August 2012 at the plastic house of Babakan Sawah Baru Experimental Station, IPB. The experiment was arranged in randomized block design with two factors, namely nitrogen fertilizer and type of bacteria. The dosage of N fertilizer (urea), i.e. 0, 50, 75 and 100 kg N ha-1. The types of bacteria, i.e. without bacteria, Azotobacter-like, Azospirillum-like, and consortium. The result of the experiment showed that N fertilization significantly affected to all variables except the percentage of empty grains per panicle, 1,000 grain weight, and N content of plant. The types of bacteria significantly affected to root dry weight, number of filled grain per panicle, greenness of leaf, uptake and content of nitrogen (shoot and grain), grain weight per plot. Consortium of bacteria were capable to reduce 25% the use of inorganic N fertilizer from recommendation doses (100 kg N ha-1) that based on the value of agronomic effectiveness.Keywords: Azospirillum-like, Azotobacter-like, consortium of bacteria
Identifikasi dan Potensi Cendawan Indigenous untuk Pelapukan Batang Kelapa Sawit di Bogor, Indonesia Nofrifaldi; Hariyadi; Widyastuti, Rahayu
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 3 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.215 KB) | DOI: 10.24831/jai.v47i3.26040

Abstract

Indigenous microorganisms effectively accelerate the decomposition of agricultural waste and agricultural waste that has been decomposed properly can be an organic fertilizer containing macro and microelements. The aim of the study was to determine the potential of indigenous cellulolytic fungi in the palm oil stems after replanting. The research was conducted at the Cikabayan Experimental Field Bogor Agricultural University, Dramaga, West Java. The experiment was conducted in October 2018 until January 2019, using a randomized complete block design consisting of four decomposition methods treatment: control, added with decomposer, chopping, and chopping+decomposer. The results showed that four isolates and isolate A were chosen to calculate the number of fungi at a dilution rate of 10-6 because they had the largest diameter. The identification results of isolate A were Trichoderma harzianum fungi and it was concluded that Trichoderma sp. in the palm oil stem after replanting, it has the potential as an indigenous cellulolytic fungus of oil palm trunks. The chopping+decomposer treatment can accelerate the weathering of the oil palm stem with a significant influence between the treatments that have met the requirements of compost maturity as specified in SNI 19-7030-2004 with ratio C/N 16.11 at 3 months after treatment. Keywords: decomposer, Elaeis guineensis Jacq., isolate, Trichoderma sp.
THE EFFECT OF COMBINATION SLUDGE, HUSK CHARCOAL, COMPOST, AND NPK FERTILIZER ON THE GROWTH SWEET CORN Fitriyani, Indri Hapsari; Widyastuti, Rahayu; Aprilianti, Nabila
Agrisaintifika: Jurnal Ilmu-Ilmu Pertanian Vol. 9 No. 3 (2025): Agrisaintifika
Publisher : Universitas Veteran Bangun Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32585/ags.v9i3.7112

Abstract

The spice and seasoning industry produce sludge waste that has the potential to pollute the environment if not managed properly. This waste contains nutrients and organic matter, making it a potential soil conditioner for sweet corn cultivation; however, its use must be combined with other materials such as rice husk charcoal and compost. This study aims to determine the effectiveness of combining sludge, compost, husk charcoal, and NPK fertilizer on the growth and productivity of sweet corn. This study was conducted at the Cikabayan Experimental Farm using a Randomized Block Design (RBD) with five levels of sludge application: (1) 100% sludge (S1H2), (2) 75% sludge (S2H2), (3) 50% sludge (S3H2), all treatments were combined with husk charcoal (75%), compost (30%), NPK fertilizer (75%), urea (100%), (4) PB (NPK 100%), and (5) Control (no treatment). Each treatment was repeated three times, resulting in 15 experimental units. The results showed that the S2H2 treatment yielded the best results for all plant growth parameters, such as plant height, stem diameter, and all generative parameters. The S1H2 combination yielded the best results for soil chemical and biological characteristics, including pH, organic carbon, total nitrogen, total microbes, and total fungi. The S1H2 combination also yielded the best results for corn plant productivity at 5.30 tons/ha. The combination of organic matter and NPK fertilizer application is highly efficient as it reduces NPK fertilizer use by 25%.
Optimization of Empty Fruit Bunch Application to Improve Soil Organic Carbon, Total Soil Nitrogen, Earthworm Populations, and Oil Palm Performance on Spodosols Adhy Ardiyanto; Suria Darma Tarigan; Rahayu Widyastuti; Budi Nugroho; Fathan Aldi Rivai
Caraka Tani: Journal of Sustainable Agriculture Vol 41, No 2 (2026): April
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v41i2.110106

Abstract

Using oil palm empty fruit bunches (EFB) as an organic amendment is a crucial strategy to improve soil quality in oil palm plantations, particularly on nutrient-poor Spodosol soils. This study aimed to evaluate the effects of 2 EFB application methods (incorporation into the soil and surface mulching) on soil organic carbon (SOC), nitrogen content, earthworm populations as soil biological indicators of fertility, and oil palm performance. A 12-month field experiment was conducted with observations every 4 months, using a completely randomized design (CRD) with 3 treatments and 9 replications each (n = 27 experimental units). The treatments included no EFB application (T0), EFB incorporated into the soil (T1), and EFB applied as surface mulch (T2). The results showed that both EFB application methods significantly increased SOC compared to the control, with the highest SOC observed in T2 (2.30±0.34%) after 12 months. Earthworm populations also increased progressively, reaching 19.33 individuals m-2 in T2. Pearson correlation analysis revealed a strong positive relationship between SOC content and earthworm abundance (r = 0.60; p < 0.001). These results confirm that EFB application, especially through surface mulching, is an effective and sustainable approach to enhancing soil quality, biological activity, and ecosystem functioning on Spodosols in oil palm plantations.
Co-Authors , Giyanto . Anandyawati . Giyanto A M Zuhud, Ervizal Abda Abda Abied Khafidhan Adhy Ardiyanto Adiyaksa, Ivan Khofian AHMAD JUNAEDI Ana Khalisha Anandyawati, . Anas Iswandi Andreas Panjaitan Andreas Panjaitan Ania Cintaresmini Ania Citraresmini Ania Citraresmini Antonius Suwanto Aprilianti, Nabila Apsari Putri Wulandari Aris Tri Wahyudi Atang Sutandi Atit Kanti Aviantara, Dwindrata B. Azra Zahrah Nadhirah Ikhwani Bria, Deseriana Budi Nugroho Budi Nugroho Budi Nugroho Dadang . DADANG DADANG Daniel Happy Putra Darmawan Darwis Delima Napitupulu Delima Napitupulu Devanda Ayu Lidya Permata Putri Dewi Sekar Pengerteni Dhanti Hanifa Muslimah Dimas Syahiddin DWI ANDREAS SANTOSA Dwi Andreas Santosa Dwi Wulandari Edy Hartulistiyoso Eka Lupitasari Endang Sulistyorini Endang Sulistyorini Enny Widyati Enok Sumarsih ERNIN HIDAYATI Erwinda Erwinda Evi Ambarsari Fahrizal Hazra Fajri, Ihsanul Fany Juliarti Panjaitan Fathan Aldi Rivai Fitriyani, Indri Hapsari Giyanto Gunawan Djajakirana Hariyadi Heru Bagus Pulunggono I Made Sudiana I Nyoman Sujana Ida Widiyawati Idris Idris Idris, Komaruddin Irawati, Arfi Irdika Mansur Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Chaniago Jekvy Hendra Jekvy Hendra Joko Warino Kartika, Sari Yulia Khairiyah, Yaumil Laksmita Prima Santi MAIPA DIA PATI Masaru Kobayashi Misbakhul Bait Nabila Dianisa Niken Puspita sari Nofrifaldi Ohiwal, Morgan Priyo Cahyono Putri Wulandari, Dwi Ratnawati Lantifasari Remila Selvany Risa Rosita Rohani Cinta Badia Ginting Rumella Simarmata Rury Kurniawan Safira Eka Aprianti Sanjaya, Wilhelmus Terang Arga Sarah Sakinah Umadi Sari Yulia Kartika SATRIYAS ILYAS Satya Nugroho Soetanto Abdoellah Sri Sulisawati Suciati, Fuzi Sugeng Santoso Sugiarto, Petrus Sugiyanta , Sukmadewi, Desak Ketut Tristiana Sumarsih, Enok Suria Darma Tarigan Suryo Wiyono Syaiful Anwar Tita Puspitasari Titik Tri Wahyuni Umi Hidayati Umi Hidayati Utami, Aditya Dyah Widrializa, . Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Winda Ika Susanti Wiwik Eko Widayati Yayuk R. Suhardjono Yayuk Rahayuningsih Suhardjono Yayuk Rahayuningsih Suhardjono Yuni Lisafitri, Yuni Yusuf, Sri Malahayati Zuhud, Evrizal A M