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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia MEDIA KONSERVASI Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Jurnal Ilmiah Aplikasi Isotop Radiasi Microbiology Indonesia Journal of Tropical Soils Jurnal Penelitian Hutan Tanaman Pelita Perkebunan Journal of Degraded and Mining Lands Management Jurnal Sumberdaya Hayati ZOO INDONESIA Jurnal Penelitian Karet AGRISAINTIFIKA Jurnal Ilmu-ilmu Pertanian Jurnal Bioteknologi & Biosains Indonesia (JBBI) SAINS TANAH - Journal of Soil Science and Agroclimatology Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Journal of Tropical Crop Science Jurnal Entomologi Indonesia Agroekoteknologi Tropika Lembab Agro Bali: Agricultural Journal Jurnal Kesehatan Jurnal Tanah dan Iklim Zoo Indonesia Prosiding Seminar Nasional Biologi
RAHAYU WIDYASTUTI
Departemen Ilmu Tanah Dan Sumberdaya Lahan, Fakultas Pertanian IPB, Jl. Meranti Kampus IPB Dramaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Other

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Design and Testing of Stream as a Sterilization Tool for Trichoderma sp. Propagation Media Using a Palm Oil Mill Steam Boiler Fajri, Ihsanul; Hartulistiyoso, Edy; Widyastuti, Rahayu
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 14, No 2 (2025): April 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i2.570-581

Abstract

Trichoderma sp. is a fungus used in oil palm plantations to control basal stem rot disease, which can reduce palm oil production by up to 80%. At PT. Bumitama Gunajaya Agro, the production of this fungus uses an autoclave, which can only produce 51 kg of Trichoderma sp. per day per unit. To meet the high demand for Trichoderma sp., a large-capacity sterilization tool called "stream" is needed for mass production. The optimal performance of the Stream shows that effective sterilization can be achieved in 20 min when the tool is operated for 45 min. The effectiveness of this sterilization time is proven by the Total Plate Count (TPC) test, which shows a bacterial count of 109×103 CFU/g and a fungal count of 35 CFU/g. The Trichoderma sp. product produced through this process has a conidium density of 8×108, 100% conidium viability, and an inhibition power of 54%, all of which exceed the standard values of SNI 8027.3:2014. Based on its production capacity, Stream can achieve production of up to 1 ton per day with quality not significantly different from Trichoderma sp. production using an autoclave. Keywords: Autoclave, Basal stem rot disease, Sterilization, TPC, Trichoderma sp.
Effect of Bio-Organic Fertilizer on Soil Organism in Experimental Oil Palm Plantation Cikabayan, Bogor, Indonesia Widyastuti, Rahayu; Adiyaksa, Ivan Khofian; Wulandari, Dwi; Zuhud, Evrizal A M
Jurnal Ilmu Tanah dan Lingkungan Vol 27 No 1 (2025): Journal of Soil Science and Environment
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.27.1.7-15

Abstract

The use of chemical-based fertilizer continuously without addition of organic matter may have hazardous effects on the environment. Numerous studies have shown that chemical-based fertilizers may disrupt the balance of soil properties, including physical, chemical, and biological properties. Bio-organic fertilizers have become one of the alternative answers to oil palm sustainability and a replacement for chemical-based fertilizers to promote and provide a better service to the soil and environment. This study aims to analyze the effect of application of biofertilizers combined with different dosages of organic matter (bio-organic fertilizers), i.e. 0 kg tree-1, 6 kg tree-1, and 12 kg tree-1, respectively. The study was conducted in Cikabayan Experimental Oil Palm Plantation, Bogor, West Java with 12 year-old-plant oil palm, from May to November 2023. The study showed an increase in the population density of the soil organisms after application of bio-organic fertilizers, especially with the dose of 6 kg tree-1. The change in the soil fauna community structure was also observed in this study, where Collembola dominated the ecosystem after fertilization. These findings offer practical strategies to mitigate the negative impacts of traditional fertilizers. Moreover, the study highlights the role of bio-organic fertilizers in enhancing soil biodiversity by increasing the populations of beneficial soil organisms.
Study of Root Exudate Organic Acids and Microbial Population in the Rhizosphere of Oil Palm Seedling Anandyawati, .; Sumarsih, Enok; Nugroho, Budi; Widyastuti, Rahayu
JOURNAL OF TROPICAL SOILS Vol. 22 No. 1: January 2017
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2017.v22i1.29-36

Abstract

Mutual interaction between plants and microbes occured in the rhizosphere is expected to increase productivity of crops or soil fertility for agriculture. Plants excrete root exudates to attract microbes, and then microbes obtain habitat and food supply from plants and can fulfill the nutrient requirements through assisted enzymatic activity. The objective of the research was to study the types and amounts of root exudate organic acids, microbial population, and the relationship between root exudate organic acids and microbial population in the rhizosphere of oil palm seedlings. The study was conducted in a greenhouse using a planting medium of sterile quartz sand. The study was conducted using two factorials completely randomized design with three replications. The first factor was oil palm seedling age (control / no oil palm seed, 1, 3, 6, 9 and 12 months-old of oil palm seedlings) and the second factor was the periods of seedling growth (45, 90, 135 and 180 days), so in total there were 72 experimental units. The result of High Pressure Liquid Chromatography (HPLC) analysis revealed that four kinds of organic acids were observed in the rhizosphere of oil palm seedlings, with the highest concentration were: acetic acid (1.66 ppm), citric acid (0.157 ppm), malic acid (2.061 ppm) and oxalic acid (0.675) ppm. The highest total population of microbes, fungi, Azotobacter, phosphate solubilizing bacteria (PSB) and phosphate solubilizing fungi (PSF) were 19.38 × 106 cfu g-1 soil, 3.28 × 104 cfu g-1 soil, 12.09 × 105 cfu g-1 soil, 8.39 × 104 cfu g-1 soil and 1.15 × 104 cfu g-1 soil, respectively. There are positive correlations between root exudate organic acids and total microbes, fungi, Azotobacter, PSB and PSF are.Keywords: microbes, organic acids, rhizosphere, root exudates
The Diversity and Abundance of Springtail (Collembola) on Forests and Smallholder in Jambi Widrializa, .; Widyastuti, Rahayu; Santosa, Dwi Andreas; Djajakirana, Gunawan
JOURNAL OF TROPICAL SOILS Vol. 20 No. 3: September 2015
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2015.v20i3.173-180

Abstract

Harapan forests is the first restoration forest in Indonesia, includes several different ecosystems. Different ecosystems have different characteristic to affect the diversity and abundance of Collembola. In the ecosystem, Collembola have an important role in biocontrol, decomposition, soil nutrient distribution, stimulation of soil microbial activity and as an alternative feed for predators. This study was aimed to investigate the diversity and abundance of Collembola in four ecosystems at Harapan forest, i.e. secondary forest, rubber forest, rubber smallholder and oil palm smallholder. To achieve the objective, soil samples were taken at 48 observation points in the four ecosystem. The soil samples were then extracted by Kempson Extractor. The diversity and abundance of Collembola in four ecosystems were categorized as medium to high. The total of individual and population of Collembola in the secondary and rubber forest ecosystems were likely to be higher than in the rubber and oil palm smallholders. This study had also identified four orders, 14 families and 31 genera of Collembola, where Isotomiella and Proisotoma dominated the genus of Collembola in the four ecosystems. [How to Cite: Widrializa, R Widyastuti, DA Santosa and G Djajakirana. 2015. The Diversity and Abundance of Springtail (Collembola) on Forests and Smallholder in Jambi. J Trop Soils 20: 173-180. Doi: 10.5400/jts.2015.20.3.173] 
Effect Carrier Materials of Bradyrhizobium sp. strain PZS_A08 on Growth of Indigofera zollingeriana Sanjaya, Wilhelmus Terang Arga; Kartika, Sari Yulia; Sukmadewi, Desak Ketut Tristiana; Widyastuti, Rahayu; Anas, Iswandi
JOURNAL OF TROPICAL SOILS Vol. 26 No. 2: May 2021
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2021.v26i2.95-103

Abstract

Sustainable forage production is needed to meet animal feed needs as the livestock industry increases. The purpose of this study is to evaluate the effect of liquid and solid carrier material on the effectiveness and infectivity of Bradyrhizobium sp. strain PZS_A08 on the growth of Indigofera zollingeriana (I. zollingeriana). The two carrier materials used were zeolite (solid) and molasses (liquid). This study used two carrier materials consisting of zeolite (solid) and molasses (liquid). The five treatments given were P0 (50% NPK), P1 (50% NPK+liquid biofertilizer), P2 (50% NPK+sterile liquid biofertilizer), P3 (50% NPK+solid biofertilizer), P4 (50 % NPK+sterile solid biofertilizer), P5 (100% NPK). Observations were made on the plant's height, number of leaves, number of nodules, upperparts and root wet biomass, root and upper parts dry biomass, root length, and microbial population). The use of liquid and solid carrier materials effectively affected the effectiveness and infectivity of Bradyrhizobium sp. strain PZS_A08 on  I. zollingeriana. Inoculants Bradyrhizobium sp. strain PZS_A08 significantly increased plants' growth and reduced the use of 50% NPK fertilizer. Cold storage temperature (5ºC) effectively maintained Bradyrhizobium sp. strain PZS_A08 on liquid and solid carriers, while solid carriers showed better effectiveness at room temperature storage (30oC). Through this research, solid carriers such as zeolite are recommended as carriers for Bradyrhizobium sp. filter PZS_A08.
Isolation and Characterization of Propoxur-Degrading Bacteria, Brucella pseudintermedia LED 6 from a Pineapple Plantation in Lampung Hidayat, Taufiq; Widyastuti, Rahayu; Djajakirana, Gunawan; Antonius, Sarjiya; Dewi, Tirta Kumala
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 4 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.4.725

Abstract

Propoxur is a non-systemic carbamate insecticide that is widely used in Indonesia to control insect pests. However, its persistence and toxicity pose environmental concerns. Bioremediation with bacteria is a viable method for mitigating the detrimental effects of propoxur residues. The goal of this work was to isolate and characterize bacterial strains that can degrade propoxur. Isolation by enrichment culture procedures, utilizing propoxur as the sole carbon source. The isolates' morphological and physiological features were examined, and their degradation potential was determined. Six bacterial samples were isolated from a pineapple plantation in Lampung, Indonesia, and one strain, known as LED 6, showed great potential for propoxur breakdown. Molecular identification with 16S rRNA gene sequencing identified the isolate as Brucella pseudintermedia. Growth characterisation revealed that the isolate performed best at 28 °C and pH 7. After 72 hours of incubation with 500 ppm propoxur, LED 6 had deteriorated around 26% of the starting concentration. Keywords: bioremediation, Brucella pseudintermedia, pineapple, propoxur
Karakterisasi dan Viabilitas Bakteri Penambat Nitrogen dan Bakteri Pelarut Fosfat dalam Media Pembawa Biochar Ilyas, Satriyas; Widyastuti, Rahayu; Umadi, Sarah Sakinah
Jurnal Ilmu Tanah dan Lingkungan Vol 25 No 2 (2023): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.25.2.40-45

Abstract

A consortium of nitrogen-fixing bacteria and phosphate solubilizers serves as biological fertilizers to increase the availability of plant nutrients such as nitrogen (N) and phosphorus (P). The experiment aims to study the character of nitrogen-fixing bacteria and phosphate solubilizing bacteria and compare their viability on the biochar (made from husk charcoal and corn cobs) as the carrier media. The experiment involved several tests, such as pathogenicity, nitrogen-fixing ability, phosphate solubilization ability on solid Pikovskaya media, compatibility, and viability. The results showed that KPB4 isolates were pathogenic to animals or humans. Nitrogen-fixing bacteria isolates, namely KBP1, KBP2, and KBP5, had N solubility (ppm) of 54.86, 77.79, and 76.28, respectively, and had NH3 concentrations (mg/L) of 66.61, 94.46, and 92.63, respectively. BPF9 (phosphate solubilizing bacteria isolate) had a phosphate solubilization index of 1.14. Each isolate of nitrogen-fixing bacteria was compatible with BPF9. The results showed that KBP2 and KBP5 each consorted with BPF9 on corncob biochar carriers had higher bacteria populations (15.50 x 107 CFU/g) after four weeks of storage. Keywords: nitrogen-fixing, phosphate solvent, consortium bacteria, biochar
Analisis Korelasi Sifat Biologi, Kimia dan Fisika Tanah pada Berbagai Ketinggian Tempat di Bandung, Jawa Barat Rahayu Widyastuti; Sri Malahayati Yusuf; Apsari Putri Wulandari; Fitriyani, Indri Hapsari
Jurnal Ilmu Tanah dan Lingkungan Vol 25 No 2 (2023): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.25.2.64-70

Abstract

Kabupaten Bandung memiliki ketinggian tempat antara 675 mdpl – 2,100 mdpl dengan suhu udara berkisar 14oC sampai dengan 30oC. Perbedaan ketinggian tempat dapat menimbulkan perbedaan iklim dan cuaca sehingga dapat mempengaruhi transformasi hara dan populasi mikrob di dalam tanah. Penelitian ini bertujuan untuk mengetahui korelasi sifat biologi dan kimia tanah, serta kaitannya antara sifat kimia dan fisika tanah pada berbagai ketinggian tempat di Bandung, Jawa barat. Pengambilan sampel tanah menggunakan metode Purposive Random Sampling dengan 5 kali ulangan. Sampel tanah terganggu diambil pada kedalaman 0-20 cm sebanyak ±1 kg, sedangkan contoh tanah utuh diambil menggunakan ring sampler. Pengambilan sampel tanah meliputi empat ketinggian tempat yaitu ketinggian 600 mdpl penggunaan lahan tanaman kol, ketinggian 1000 dan 1200 mdpl penggunaan lahan tanaman selada, serta ketinggian 1400 mdpl penggunaan lahan perkebunan kopi. Hasil analisis menunjukkan bahwa pada ketinggian 1000 mdpl populasi bakteri pelarut fosfat (BPF) dapat tumbuh optimum sebesar 93.6 x 103 CFU g-1 dengan pH tanah agak masam, P-Total sangat tinggi, dan P-Tersedia sedang. Populasi fungi dapat tumbuh optimum sampai ketinggian 1200 mdpl. Total populasi fungi berkorelasi tinggi dengan unsur hara P-Total dan N-Total di dalam tanah. Respirasi tanah berkorelasi positif dengan semua parameter yang berhubungan dengan sifat kimia tanah. Nilai bobot isi terendah pada ketinggian 1200 mdpl bersesuaian dengan nilai C-organik dan porositas tertinggi pada ketinggian tersebut.
Design and Testing of Stream as a Sterilization Tool for Trichoderma sp. Propagation Media Using a Palm Oil Mill Steam Boiler Fajri, Ihsanul; Hartulistiyoso, Edy; Widyastuti, Rahayu
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 2 (2025): April 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i2.570-581

Abstract

Trichoderma sp. is a fungus used in oil palm plantations to control basal stem rot disease, which can reduce palm oil production by up to 80%. At PT. Bumitama Gunajaya Agro, the production of this fungus uses an autoclave, which can only produce 51 kg of Trichoderma sp. per day per unit. To meet the high demand for Trichoderma sp., a large-capacity sterilization tool called "stream" is needed for mass production. The optimal performance of the Stream shows that effective sterilization can be achieved in 20 min when the tool is operated for 45 min. The effectiveness of this sterilization time is proven by the Total Plate Count (TPC) test, which shows a bacterial count of 109×103 CFU/g and a fungal count of 35 CFU/g. The Trichoderma sp. product produced through this process has a conidium density of 8×108, 100% conidium viability, and an inhibition power of 54%, all of which exceed the standard values of SNI 8027.3:2014. Based on its production capacity, Stream can achieve production of up to 1 ton per day with quality not significantly different from Trichoderma sp. production using an autoclave. Keywords: Autoclave, Basal stem rot disease, Sterilization, TPC, Trichoderma sp.
Peran Bakteri Penambat Nitrogen untuk Mengurangi Dosis Pupuk Nitrogen Anorganik pada Padi Sawah Widiyawati, Ida; ,, Sugiyanta; Junaedi, Ahmad; Widyastuti, Rahayu
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 2 (2014): Jurnal agronomi indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.112 KB) | DOI: 10.24831/jai.v42i2.8424

Abstract

ABSTRACTThe availability of nitrogen in soil is one of the limiting factors to support growth and rice productivity. Nitrogen-fixing bacteria have ability to utilize air nitrogen so it becomes available in the soil. The use of nitrogen-fixing bacteria could potentially reduce application of nitrogen fertilizer. The aim of the experiment was to determine the role of nitrogen-fixing bacteria in reducing inorganic N fertilizer on lowland rice. The research was conducted in April-August 2012 at the plastic house of Babakan Sawah Baru Experimental Station, IPB. The experiment was arranged in randomized block design with two factors, namely nitrogen fertilizer and type of bacteria. The dosage of N fertilizer (urea), i.e. 0, 50, 75 and 100 kg N ha-1. The types of bacteria, i.e. without bacteria, Azotobacter-like, Azospirillum-like, and consortium. The result of the experiment showed that N fertilization significantly affected to all variables except the percentage of empty grains per panicle, 1,000 grain weight, and N content of plant. The types of bacteria significantly affected to root dry weight, number of filled grain per panicle, greenness of leaf, uptake and content of nitrogen (shoot and grain), grain weight per plot. Consortium of bacteria were capable to reduce 25% the use of inorganic N fertilizer from recommendation doses (100 kg N ha-1) that based on the value of agronomic effectiveness.Keywords: Azospirillum-like, Azotobacter-like, consortium of bacteria
Co-Authors . Anandyawati . Giyanto . Giyanto A M Zuhud, Ervizal Abied Khafidhan Adiyaksa, Ivan Khofian AHMAD JUNAEDI Ana Khalisha Anandyawati, . Anas Iswandi Andreas Panjaitan Andreas Panjaitan Andreas Panjaitan, Andreas Ania Cintaresmini Ania Citraresmini Ania Citraresmini Antonius Suwanto Aprilianti, Nabila Apsari Putri Wulandari Aris Tri Wahyudi Atang Sutandi Atit Kanti Aviantara, Dwindrata B. Azra Zahrah Nadhirah Ikhwani Bria, Deseriana Budi Nugroho Budi Nugroho Dadang . DADANG DADANG Daniel Happy Putra Darmawan Darwis Delima Napitupulu Delima Napitupulu Devanda Ayu Lidya Permata Putri Dewi Sekar Pengerteni Dewi, Tirta Kumala Dhanti Hanifa Muslimah Dimas Syahiddin Dwi Andreas Santosa DWI ANDREAS SANTOSA Dwi Wulandari Edy Hartulistiyoso Eka Lupitasari Endang Sulistyorini Endang Sulistyorini Enny Widyati Enok Sumarsih ERNIN HIDAYATI Erwinda Erwinda Evi Ambarsari Fahrizal Hazra Fajri, Ihsanul Fany Juliarti Panjaitan Fitriyani, Indri Hapsari Giyanto Gunawan Djajakirana Hariyadi Heru Bagus Pulunggono I Made Sudiana I Nyoman Sujana Ida Widiyawati Idris Idris Idris, Komaruddin Irawati, Arfi Irdika Mansur Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Chaniago Jekvy Hendra Jekvy Hendra Joko Warino Kartika, Sari Yulia Khairiyah, Yaumil Laksmita Prima Santi MAIPA DIA PATI Masaru Kobayashi Misbakhul Bait Nabila Dianisa Niken Puspita sari Nofrifaldi Ohiwal, Morgan Priyo Cahyono Putri Wulandari, Dwi Ramadhani Sri Astuti, Andi Febrianti Ratnawati Lantifasari Remila Selvany Risa Rosita Rohani Cinta Badia Ginting Rumella Simarmata Rury Kurniawan Safira Eka Aprianti Sanjaya, Wilhelmus Terang Arga Sari Yulia Kartika Sarjiya Antonius SATRIYAS ILYAS Satya Nugroho Soetanto Abdoellah Sri Sulisawati Suciati, Fuzi Sugeng Santoso Sugiarto, Petrus Sugiyanta , Sukmadewi, Desak Ketut Tristiana Sumarsih, Enok Suryo Wiyono Syaiful Anwar Taufiq Hidayat Tita Puspitasari Titik Tri Wahyuni Umadi, Sarah Sakinah Umi Hidayati Umi Hidayati Utami, Aditya Dyah Widrializa, . Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Winda Ika Susanti Wiwik Eko Widayati Yayuk R. Suhardjono Yayuk Rahayuningsih Suhardjono Yayuk Rahayuningsih Suhardjono Yuni Lisafitri Yusuf, Sri Malahayati Zuhud, Evrizal A M