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Kualitas dan Produksi Vermikompos Menggunakan Cacing African Night Crawler (Eudrilus eugeniae): Quality and Production of Vermicompost Using African Night Crawler Worms (Eudrilus eugeniae) Fahrizal Hazra; Nabila Dianisa; Rahayu Widyastuti
Jurnal Ilmu Tanah dan Lingkungan Vol 20 No 2 (2018): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (308.341 KB) | DOI: 10.29244/jitl.20.2.77-81

Abstract

Adding organic fertilizer such as vermicompost can be done to overcome the deficiency problem of soil organic matter. One of the worm species which can be used in vermicomposting is Eudrilus eugeniae. Purpose of this research was to know how the combination of cow dung and rice straw impacts vermicompost quality from organic carbon, N, P, total K, urease enzyme activity and vermicompost production. Treatment that used in this research is randomized completely design with 4 treatments. The treatments are P1: feed 400 g of cow dung, P2: feed 400 g of rice straw, P3: feed 280 g of cow dung and 120 g of rice straw, and P4: feed 140 g of cow dung and 60 g of rice straw. The research result shows that real treatment impacts all parameters on the produced vermicompost. Production result is determined by ratio value of output/input (O/I). Quality and production of vermicompost on P4 is a treatment with the best result. It is because the combination of cow dung and rice straw gives a relatively complete macro nutrient result and the higher feed dose will gives higher vermicompost production.
Peran Kompos dan Mikoriza pada Pertumbuhan Tanaman Tomat (Solanum lycopersicum) di Tanah Berpasir Fahrizal Hazra; Dimas Syahiddin; Rahayu Widyastuti
Jurnal Agroekoteknologi Tropika Lembab Vol 4, No 2 (2022): Agroekoteknologi Tropika Lembab Volume 4 Nomor 2 Februari 2022
Publisher : Mulawarman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35941/jatl.4.2.2022.7003.113-122

Abstract

Kompos merupakan bahan pembenah tanah (amelioran) yang umum digunakan dan dapat ditingkatkan kualitasnya dengan penambahan mikrob fungsional seperti mikoriza. Mikoriza adalah kelompok fungi yang dapat bersimbiosis dengan tanaman pada sistem perakaran. Penelitian ini bertujuan menganalisis pengaruh pemberian kompos dan mikoriza terhadap pertumbuhan tanaman tomat (Solanum lycopersicum) pada tanah berpasir. Penelitian ini dilakukan dengan menggunakan Rancangan Acak Lengkap (RAL) dengan 11 perlakuan yang terdiri dari kontrol dan dua faktor perlakuan. Faktor pertama adalah persentase dosis kompos dan faktor kedua adalah dosis mikoriza. Faktor persentase dosis kompos terdiri atas 150% kompos (K1), 100% kompos (K2), 75% kompos (K3), 50% Kompos (K4), dan 25% Kompos (K5), sedangkan faktor dosis mikoriza terdiri atas 5g (M1) dan 2.5g (M2). Pertumbuhan tanaman diamati hingga fase vegetatif lalu dilakukan analisis kimia dan penghitungan persentase kolonisasi akar serta jumlah spora mikoriza. Perlakuan 150% kompos dengan dosis 2.5g mikoriza memilik nilai tinggi tanaman terbaik sebesar 74.70 cm dan jumlah helai daun sebanyak 314 helai. Kadar N-total tertinggi yaitu 0.30% terdapat pada kombinasi perlakuan 150% kompos dengan 5g mikoriza ataupun dengan 2.5g mikoriza. Persentase kolonisasi akar tertinggi sebesar 50% dan kadar P-tersedia tanah tertinggi yaitu 32.5 mg/100g pada perlakuan 150% kompos dengan dosis 5g mikoriza.
SIDIK CEPAT BIOKATALISASI AIR ASAM TAMBANG PADA LAHAN BEKAS TAMBANG BATUBARA Rapid Assessment of Acid Mine Drainage on Ex-coal Mining Land Enny Widyati; Rahayu Widyastuti; Ratnawati Lantifasari
Jurnal Penelitian Hutan Tanaman Vol 7, No 1 (2010): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2063.197 KB) | DOI: 10.20886/jpht.2010.7.1.51-58

Abstract

Salah satu hambatan terberat dalam kegiatan rehabilitasi lahan bekas tambang adalah air asam tambang (AAT). AAT merupakan oksidasi mineral sulfida dengan melepaskan sulfat sehingga dapat menurunkan pH tanah secara drastis. Apabila AAT dikatalis oleh kelompok bakteri pengoksidasi sulfur (BOS), maka laju dari proses tersebut akan dipercepat 500.000 - 1.000.000 kali lipat. Oleh karena itu penelitian ini bertujuan untuk mengetahui aktivitas biokatalisasi pemasaman lahan bekas tambang batubara. Aktivitas biokatalisasi dapat diketahui dengan cepat (2 - 3 hari) melalui uji biological acid producing potential (BAPP). Untuk meyakinkan uji BAPP ini pada tanah baik yang memberikan hasil positif maupun negatif dilakukan isolasi BOS, sebagai kontrol adalah media tanpa tanah. Hasil uji menunjukkan bahwa dari 24 contoh tanah yang diuji 22 contoh tanah (91% ) memberikan hasil positif. Hal ini ditunjukkan dengan pH kultur selalu di bawah 3,5 yang mengindikasikan bahwa proses pemasaman lahan dikatalis secara biologi oleh bakteri. Pengujian katalisasi biologis oleh bakteri menggunakan uji BAPP mempunyai akurasi 80%. Salah satu bakteri yang ditemukan mengkatalis pemasaman lahan adalah Thiobacillus ferrooxidans.
Peningkatan Kemampuan Mikroba Pelarut Fosfat dan Kalium Melalui Teknik Mutasi Iradiasi Gamma Desak Ketut Tristiana Sukmadewi; Iswandi Anas; Rahayu Widyastuti; Ania Citraresmini
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 15, No 2 (2019): Desember 2019
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.198 KB) | DOI: 10.17146/jair.2019.15.2.4662

Abstract

Iradiasi gamma merupakan salah satu alternatif untuk memicu mutasi yang dapat menginduksi peningkatan kemampuan mikroba pelarut fosfat dan kalium. Tujuan dari penelitian ini adalah  untuk mempelajari pengaruh iradiasi gamma terhadap mikroba pelarut fosfat dan kalium, mempelajari perubahan kemampuan mutan mikroba dalam  melarutkan fosfat dan kalium, serta perubahan pada tingkat molekuler yang terjadi akibat mutasi iradiasi gamma Metode penelitian terdiri dari iradiasi mikroba menggunakan sinardengan dosis 0; 1; 2,5; 5; 7,5; 10; 15 kGy, uji kemampuan mikroba dalam melarutkan fosfat dan kalium setelah iradiasi serta pengujian pada tingkat molekuler. Hasil yang didapatkan pada penelitian ini adalah iradiasi gamma  memberikan pengaruh terhadap jumlah populasi dan kemampuan mikroba dalam melarutkan fosfat dan kalium. Hal ini menunjukkan bahwa sinar gamma dengan dosis 1 kGy sampai 15 kGy menurunkan populasi bakteri dan fungi. Semakin tinggi dosis iradiasi gamma jumlah sel yang mati meningkat. Umumnya iradiasi dengan sinar gamma menghasilkan mutan dengan kemampuan melarutkan P dan K yang menurun. Akan tetapi beberapa dosis mampu meningkatkan kemampuan mutan dalam melarutkan fosfat dan kalium. Mutan  BPK5 pada dosis 7,5 kGy mampu melarutkan fosfat (165,67 ppm) dan kalium (18,89 ppm) yang lebih tinggi dibandingkan kontrol. Mutan FPF 4 pada dosis 2,5 kGy mampu melarutkan fosfat (418,15 ppm) lebih tinggi dibandingkan kontrol, sedangkan mutan FPF 4 mampu melarutkan kalium (13,90 ppm) lebih tinggi dibandingkan kontrol pada dosis 15 kGy. Perubahan pada tingkat molekuler diindikasikan dengan terjadinya perubahan basa pada sekuen DNA antara isolat induk (tanpa iradiasi) dengan sekuen mutan. Pada bakteri mutasi tertinggi terjadi pada transisi adenin menjadi guanin dan transversi timin menjadi sitosin dengan persentase masing-masing terhadap total perubahan sebesar 23,91 %. Perubahan basa pada sekuen DNA  isolat mutan fungi ditunjukkan dengan terjadinya  insersi adenin dan timin dengan persentase masing-masing terhadap perubahan total sebesar 50 %.
Pengaruh Super Water Absorbent (SWA) Hasil Iradiasi Gamma dan Frekuensi Penyiraman terhadap Pertumbuhan Caisim (Brassica Juncea L.) dan Populasi Mikroba Tanah Evi Ambarsari; Anas Iswandi; Rahayu Widyastuti; Darmawan Darwis; Tita Puspitasari; Dewi Sekar Pengerteni; Sri Sulisawati
Jurnal Ilmiah Aplikasi Isotop dan Radiasi Vol 14, No 2 (2018): Desember 2018
Publisher : BATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.627 KB) | DOI: 10.17146/jair.2018.14.2.4654

Abstract

Super Water Absorbent (SWA) merupakan polimer hidrogel yang mempunyai struktur tiga dimensi dan dapat menyerap air lebih dari 100 kali berat keringnya. SWA tidak larut dalam air tetapi dapat mengembang (swelling). SWA dapat meningkatkan kelembaban tanah sehingga lingkungan mikroba tanah menjadi lebih baik. Aplikasi SWA dari pati singkong sangat diperlukan dalam bidang pertanian karena SWA ini menahan air sehingga meningkatkan ketersediaan air dalam tanah dan dengan mudah dapat diuraikan oleh mikroba tanah sehingga tidak meninggalkan residu yang dapat berdampak negatif pada lingkungan. Penelitian ini bertujuan untuk mempelajari pengaruh SWA dari pati singkong hasil iradiasi gamma dan frekuensi penyiraman terhadap pertumbuhan caisim dan populasi mikroba tanah. Penelitian dilakukan di rumah kaca menggunakan pot dengan menggunakan metode percobaan Rancangan Acak Lengkap (RAL). Tiga faktor yang diteliti adalah (1) takaran SWA (0,0 g/kg tanah, 0,1 g/kg tanah dan 0,2 g kg tanah); (2) Cara penempatan SWA (melingkari tanaman dan dikonsentrasikan 4 titik sekitar tanaman); dan (3) frekuensi penyiraman (setiap hari, setiap 2 hari dan setiap 4 hari). Sintesis SWA berbasis pati singkong dengan iradiasi gamma pada dosis 10 kGy menghasilkan SWA dengan karakteristik kemampuan absorbsi air pada suhu kamar dan pH 7 hingga 150 kali berat kering dan fraksi gel 87%. Aplikasi SWA sampai dengan dosis 0,2 g/kg tanah dapat meningkatkan kadar air tanah, total populasi mikroba tanah dan fungi tanah beserta respirasi tanah namun tidak mempengaruhi pertumbuhan tanaman caisim dan tidak mengurangi frekuensi penyiraman. Teknik penempatan SWA di dalam tanah tidak mempengaruhi pertumbuhan caisim dan populasi mikroba tanah. 
Microbial proportion and heterotroph CO2 flux from drainage peatland under oil palm plantation Eka Lupitasari; Rahayu Widyastuti; Heru Bagus Pulunggono
Journal of Degraded and Mining Lands Management Vol 9, No 1 (2021)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2021.091.3055

Abstract

The difference in soil layer can affect heterotroph respiration that means CO2 fluxes from microbial decomposition in peatlands. Oil palm plants release root exudates transported to other places, i.e., shrub, by water movement, which can stimulate microbial activity. This study was conducted to learn the effects of differences of the soil layer and distance from the trunk in drainage peatland under oil palm plantation on total bacteria, fungi, cellulolytic bacteria, ligninolytic fungi, and heterotroph fluxes CO2, then compared to a shrub. Heterotroph respiration decreased with soil layer depth, where at the layer 0-20 cm released amount of CO2 as much 6.07 + 1.76, at 20-40 cm was 5.18 + 0.50, and at 40-60 cm 5.27 + 1.20 mg CO2 100 g-1 day-1, and tended higher than in shrub where a layer of 0-20 cm released 5.51 + 1.69, then decrease at 20-40 cm to 4.83 + 1.38, and at 40-60 cm 4.30 + 1.08 mg CO2 100 g-1 day-1. Total bacteria (107 CFU g-1) and fungi (105 CFU g-1) were higher than total cellulolytic bacteria (103 CFU g-1) and ligninolytic fungi (102 CFU g-1) in both under oil palm plantation and shrub. Organic acids affected the abundance of total bacteria and fungi but did not affect cellulolytic bacteria and ligninolytic fungi on both sites, as shown by a lower population and low cellulose and laccase enzymes. These findings showed that heterotroph CO2 flux tended higher in oil palm plantations and lignocellulolytic microbes are not the only source of heterotroph respiration.
Diversity of Oribatids (Acari) at different land use types in Mentebah, Kapuas Hulu, West Kalimantan Endang Sulistyorini; Rahayu Widyastuti; Sugeng Santoso
Journal of Degraded and Mining Lands Management Vol 5, No 4 (2018)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.273 KB) | DOI: 10.15243/jdmlm.2018.054.1355

Abstract

Kalimantan forests are mostly managed by human. Deforestation will affect the ecosystem and species inside. Oribatids are the most abundant soil mesofauna. They have an important role in decomposition of organic matter in the soil. Here, we investigated the effect of six different land use types (i.e. primary forest, secondary forest, jungle rubber, slash and burn, young fallow and old fallow) and evaluated the effect of environmental factors on the abundance and diversity of oribatids. The oribatids were collected using transect method along 100 m with 10 sampling points with the soil depth of 0-5 cm. Oribatids were extracted using Berlese Funnel Heat Extractor. Identification of oribatids was done to family level and diversity index was determined according to Shannon's diversity index. As much as 36 families of oribatids were recorded from the site at Mentebah, Kapuas Hulu. Oribatids in the jungle rubber showed the highest abundance (592.5 individuals/m2), followed by secondary forest (317.5 individuals/m2), primary forest (287.5 individuals/m2), slash and burn (195 individuals/m2), young fallow (157.5 individuals/m2) and old fallow (142.5 individuals/m2). The value of diversity index according to Shannon Wiener (H’) ranged between 1.71-2.64 or categorized as moderate diversity values. The results of the research showed that there were strong coefficient correlation values of some families of oribatids with soil pH, C-organic, N total, C/N ratio, water content and soil temperature
Study of Oribatids population on three types of land use at PT Nusantara VIII Plantation, Cisarua Sub-district, Bogor, West Java Deseriana Bria; Rahayu Widyastuti; Sugeng Santoso
Journal of Degraded and Mining Lands Management Vol 6, No 2 (2019)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (749.673 KB) | DOI: 10.15243/jdmlm.2019.062.1667

Abstract

Most of the forest area of PT Perkebunan Nusantara VIII have been converted to the plantation (tea, cacao, coffee) and agriculture, such as crops (intercropping of oranges and pineapple) areas. The forest conversion will affect soil biodiversity, which will reduce soil mesofauna population, such as the Oribatids group. Oribatids is one of the soil mesofauna which has a very important role in the soil as a decomposer. Because of its important role and their huge numbers in the soil, Oribatids can be used as a bioindicator of soil fertility. This study aimed to elucidate the abundance and diversity of Oribatids and the relationship between soil properties and population of Oribatids in three types of land use at PT Perkebunan Nusantara VIII. Soil and litter samples were taken in three different types of land use (tea gardens, intercropping, and secondary forests) using a simple random sampling method with a distance of each of the 15 sampling points of 100 m at a soil depth of 0-5 cm. Soil and litter samples were extracted using Berlese Funnel Heat Extractor. Oribatids identification was carried out until the family level, and the Oribatids diversity index were determined according to Shannon's diversity index. The identification results obtained in the three types of land use amounted to 24 families from 172 families found previously. The highest abundance of Oribatids was found in secondary forests both in the soil and litter layer (169.85 and 428.87 individuals/m², respectively), the lowest abundance of Oribatids was found in intercropping both in the soil and litter layer (89.17 and 229.30 individuals/m², respectively). According to Shannon Wiener (H'), the diversity index value ranges from 1.48-1.85 or it was categorized as a low-medium diversity value. The results of this study indicated that there was a positive correlation coefficient value from several families of Oribatids with soil pH, organic C, total N, C/N ratio, water content, and soil temperature.
The effectiveness of application of phosphorous and potassium solubilizing multifunctional microbes (Aspergillus costaricaensis and Staphylococcus pasteuri mutants) on maize growth Desak Ketut Tristiana Sukmadewi; Iswandi Anas; Rahayu Widyastuti; Syaiful Anwar; Ania Citraresmini
Journal of Degraded and Mining Lands Management Vol 8, No 2 (2021)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2021.082.2681

Abstract

The use of phosphorus and potassium-solubilizing microbes as biofertilizers is an alternative method to increase the availability of phosphorus and potassium in soils. This study aimed to explore the effectiveness of phosphorus (P) and potassium (K)-solubilizing multifunctional microbes (Aspergillus costaricaensis and Staphylococcus pasteuri mutants) on maize growth. The stages of this study consisted of viability test of P and K solubilizing A. costaricaensis and S. pasteuri mutants in peat and effectiveness test of P and K solubilizing A. costaricaensis and S. pasteuri mutants on maize growth. The results showed that peat carriers could keep the fungi population stable until 18 weeks of storage times. While the bacteria at 6 and 8 weeks storage times showed a slight decrease and stable in the 10 to 12 weeks storage time. The addition of P and K-solubilizing multifunctional microbes could reduce the use of fertilizer up to 50% in the treatment with a combination of easily soluble P or K sources with not-easily soluble P or K sources, as well as a combination of treatments of not-easily soluble P and K sources. This tended to occur in the treatment with the addition of A. costaricaensis mutant. 
Studi Populasi Mikrob Fungsional pada Tanah Gambut yang Diaplikasikan Dua Jenis Pestisida MAIPA DIA PATI; SYAIFUL ANWAR; RAHAYU WIDYASTUTI; DADANG .
Jurnal Sumberdaya Hayati Vol. 2 No. 1 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.1.7-12

Abstract

Agricultural land expansion necessity for food security in Indonesia has been faced by the fact in utilizing marginal land such as shallow peat soil in Central Kalimantan. Among problems encountered in such marginal land are pests and diseases incidence. Farmers practice in dealing with those problems is the chemical pesticides application. Unintended chemical pesticides that fall on/into the soil might have an adverse effect to soil microbes activity. This research aimed to assess the effect of pesticides on functional soil microbes population in peat soil. The research used completely randomized factorial design with two factors. First factor was 7 combinations of types (paraquat and buthylphenylmethyl carbamat (BPMC)) and dosages (50, 100, and 200% of recommended dosage) of pesticides. Second factor was 4 incubation times (1, 7, 14, and 28 days). Following the each of incubation times, the soil samples treated and analyzed for population of phosphate solubilizing microbes, cellulolytic microbes, and N2 fixing bacteria. The results show that phosphate solubilizing fungi population decrease by pesticides treatment. In contrast, pesticides significantly increased the Azospirillum population. Adverse effect to microbes population was found on the first day of incubation, however it recovered at 7 days and longer incubation times. In general, functional microbes population with paraquat application lower than BPMC application. 
Co-Authors . Anandyawati . Giyanto . Giyanto A M Zuhud, Ervizal Abied Khafidhan Adiyaksa, Ivan Khofian AHMAD JUNAEDI Ana Khalisha Anandyawati, . Anas Iswandi Andreas Panjaitan Andreas Panjaitan Andreas Panjaitan, Andreas Ania Cintaresmini Ania Citraresmini Ania Citraresmini Antonius Suwanto Apsari Putri Wulandari Aris Tri Wahyudi Atang Sutandi Atit Kanti Aviantara, Dwindrata B. Azra Zahrah Nadhirah Ikhwani Bria, Deseriana Budi Nugroho Budi Nugroho Dadang . DADANG DADANG Darmawan Darwis Delima Napitupulu Delima Napitupulu Devanda Ayu Lidya Permata Putri Dewi Sekar Pengerteni Dewi, Tirta Kumala Dhanti Hanifa Muslimah Dimas Syahiddin DWI ANDREAS SANTOSA Dwi Andreas Santosa Dwi Wulandari Edy Hartulistiyoso Eka Lupitasari Endang Sulistyorini Endang Sulistyorini Enny Widyati Enok Sumarsih ERNIN HIDAYATI Erwinda Erwinda Evi Ambarsari Fahrizal Hazra Fajri, Ihsanul Fany Juliarti Panjaitan Giyanto Gunawan Djajakirana Hariyadi Heru Bagus Pulunggono I Made Sudiana I Nyoman Sujana Ida Widiyawati Idris Idris Idris, Komaruddin Indri Hapsari Fitriyani Irawati, Arfi Irdika Mansur Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Iswandi Anas Chaniago Jekvy Hendra Jekvy Hendra Joko Warino Kartika, Sari Yulia Khairiyah, Yaumil Laksmita Prima Santi MAIPA DIA PATI Masaru Kobayashi Misbakhul Bait Nabila Dianisa Niken Puspita sari Nofrifaldi Ohiwal, Morgan Priyo Cahyono Putri Wulandari, Dwi Ramadhani Sri Astuti, Andi Febrianti Ratnawati Lantifasari Remila Selvany Risa Rosita Rohani Cinta Badia Ginting Rumella Simarmata Rury Kurniawan Safira Eka Aprianti Sanjaya, Wilhelmus Terang Arga Sarah Sakinah Umadi Sari Yulia Kartika Sarjiya Antonius Satriyas Ilyas Satya Nugroho Soetanto Abdoellah Sri Sulisawati Suciati, Fuzi Sugeng Santoso Sugiarto, Petrus Sugiyanta Sukmadewi, Desak Ketut Tristiana Sumarsih, Enok Suryo Wiyono Syaiful Anwar Taufiq Hidayat Tita Puspitasari Titik Tri Wahyuni Umadi, Sarah Sakinah Umi Hidayati Umi Hidayati Utami, Aditya Dyah Widrializa, . Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Wilhelmus Terang Arga Sanjaya Winda Ika Susanti Wiwik Eko Widayati Yayuk R. Suhardjono Yayuk Rahayuningsih Suhardjono Yayuk Rahayuningsih Suhardjono Yuni Lisafitri Yusuf, Sri Malahayati Zuhud, Evrizal A M