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Journal : NICHE Journal of Tropical Biology

Isolasi khamir penghasil enzim inulinase dari buah kersen (Muntingia calabura) serta pengaruh mikronutrien mangan (Mn) pada produksi enzimnya Fitri Ulfana Risky; Wijanarka Wijanarka; Sri Pujiyanto
NICHE Journal of Tropical Biology Vol. 2, No. 2, Year 2019
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5252.206 KB) | DOI: 10.14710/niche.2.2.27-37

Abstract

Kemampuan memproduksi inulinase isolat khamir hasil isolasi dari Nira Siwalan (Borassus flabellifer L.) dengan variasi konsentrasi Siti Maisaroh; Wijanarka Wijanarka; Agung Suprihadi
NICHE Journal of Tropical Biology Vol. 3, No. 1, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.553 KB) | DOI: 10.14710/niche.3.1.1-7

Abstract

Microbial inulinase is one of the most interesting enzymes, especially the ones produced by yeast. Yeast is commonly found in sugar-containing materials, such as Nira Siwalan (Borassus flabellifer L). Yeast uses simple sugar compounds in foods to obtain its energy. If the nira is stored, the fermentation begins to occur, carried by the natural microorganism of Nira Siwalan which causes sour taste as acetic acid is started to be formed. This condition is ideal for the microorganisms (such as yeast) to grow. This research aims to analyze the impact of the best calcium concentration towards the production of inulinase by indigenous yeast in Nira Siwalan (Borassus flabellifer L). The research was done in biotechnology laboratory, Biology Department, Faculty of Science and Mathematics, Diponegoro University. The activity of inulinase was determined using DNS method. The research was designed using Randomized Factorial Design with concentration of CaCl2 and incubation time as the factors. The CaCl2 concentration was differentiated into four categories: Ca0, Ca0.5, Ca1.0, and Ca1.5, while the incubation time was differentiated into 5 categories: T0, T4, T8, T12, T16, and T20. Each treatment is repeated three times. After that, the data obtained from the observation analyzed using ANOVA. The result shows that the addition of 0.5 mM CaCl2 with inulinase activity 0.555 IU/mL gives the best result.
Isolasi dan penapisan bakteri proteolitik endofit tanaman pepaya (Carica papaya L.) Lailatul Mubarokhah; Wijanarka Wijanarka; MG Isworo Rukmi
NICHE Journal of Tropical Biology Vol. 3, No. 2, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.3.2.99-104

Abstract

Pemanfaatan khamir inulinolitik indigenous ampas tebu (bagasse) sebagai penghasil biokatalis inulinase dalam produksi gula rendah kalori Moch Ali Utomo; Tia Erfianti; Dewi Ayu Maryati; Romario Dion; Pragati Wira Anggini; Wijanarka Wijanarka; Mochamad Hadi
NICHE Journal of Tropical Biology Vol. 4, No. 2, Year 2021
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.4.2.56-63

Abstract

Co-Authors Agung Suprihadi Aini, Riris Nur Anggoro, Naufal Sebastian Anto Budiharjo Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Arina Tri Lunggani Asy'ari, Mukhammad Ayuningtyas, Annisa Nur Azzahra, Meilidya Falkhiya Dewi Ayu Maryati Doktorasaintifika, Heradita Kaniaazzahra Dyah Wulandari Endang Kusdiyantini Endang Kusdiyantini Endang Kusdiyantini Endang Sutariningsih Endang Sutariningsih Endang Sutariningsih Soetarto Endang Sutariningsih Soetarto ENDANG SUTARININGSIH SOETARTO Fathika Fitrania Fauzaan, Muhammad Faishal Fitri Ulfana Risky Fransenda, Auxensius Rexer Fransisco, Theo Ginting, Ledy HARTAJANIE, LAKSMI Herida, Azalia Puspa Hermin Hermin Hermin Pancasakti Kusumaningrum Ihdina Isfara Suteja Ika Anggraini Indra Bakti Sangalang Inggrit Amedia, Inggrit K Endang Kumala Dewi Kumala Dewi Kumala Dewi Kumala Dewi Lailatul Mubarokhah Laily Kurniawati Lindayani, Lindayani Lynda - Sutrisna Maria Sarah Fadillah Maulana, Anand Reyna Maulana, Muhammad Iqbal Maulida Aglinia Mauritz Nicolaas Wattimena Mawarni, Risa Arum MG Isworo Rukmi Moch Ali Utomo Mochamad Hadi Muhammad Nur Muhammad Zainuri NANIK RAHMANI Nomeritae Nomeritae Noor Hamidah Nurhayati Nurhayati Pragati Wira Anggini Pratama Jujur Wibawa Putri S. Noor Rahmasari, Dianti Rahmasari, Dianti Rahmawati Dewi Rejeki Siti Ferniah Romario Dion Rudi Waluyo salamah salamah Sarjana Parman Sarsa A.N Shabrina, Jauhara Siti Maisaroh Siti Nur Jannah Sri Pujiyanto Sulis Setyowati, Sulis Susiana Purwantisari T. A. Lestari Tia Erfianti Tony Hadibarata, Tony Wahyuningsih, Candra YOPI YOPI Yusuf, Hadistiyani