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All Journal Jurnal Penelitian Saintek Inersia : Jurnal Teknik Sipil dan Arsitektur ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi Jurnal Akademika Biologi Teknosains: Media Informasi Sains dan Teknologi Journal of Biomedicine and Translational Research Tesa Arsitektur Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA BIOMA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi NICHE Journal of Tropical Biology Jurnal PROTEKSI (Proyeksi Teknik Sipil) Berkala Penelitian Hayati Greensphere: Journal of Environmental Chemistry Makara Journal of Science JURNAL BIOLOGI PAPUA AGRIC

Wijanarka Wijanarka

Departement Of Biology, Faculty Of Science And Mathematics, The University Of Diponegoro, Semarang Indonesia

Author-ID : 417046

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Social Sciences

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Kemampuan memproduksi inulinase isolat khamir hasil isolasi dari Nira Siwalan (Borassus flabellifer L.) dengan variasi konsentrasi Siti Maisaroh; Wijanarka Wijanarka; Agung Suprihadi
NICHE Journal of Tropical Biology Vol. 3, No. 1, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Microbial inulinase is one of the most interesting enzymes, especially the ones produced by yeast. Yeast is commonly found in sugar-containing materials, such as Nira Siwalan (Borassus flabellifer L). Yeast uses simple sugar compounds in foods to obtain its energy. If the nira is stored, the fermentation begins to occur, carried by the natural microorganism of Nira Siwalan which causes sour taste as acetic acid is started to be formed. This condition is ideal for the microorganisms (such as yeast) to grow. This research aims to analyze the impact of the best calcium concentration towards the production of inulinase by indigenous yeast in Nira Siwalan (Borassus flabellifer L). The research was done in biotechnology laboratory, Biology Department, Faculty of Science and Mathematics, Diponegoro University. The activity of inulinase was determined using DNS method. The research was designed using Randomized Factorial Design with concentration of CaCl2 and incubation time as the factors. The CaCl2 concentration was differentiated into four categories: Ca0, Ca0.5, Ca1.0, and Ca1.5, while the incubation time was differentiated into 5 categories: T0, T4, T8, T12, T16, and T20. Each treatment is repeated three times. After that, the data obtained from the observation analyzed using ANOVA. The result shows that the addition of 0.5 mM CaCl2 with inulinase activity 0.555 IU/mL gives the best result.

Isolasi dan penapisan bakteri proteolitik endofit tanaman pepaya (Carica papaya L.) Lailatul Mubarokhah; Wijanarka Wijanarka; MG Isworo Rukmi
NICHE Journal of Tropical Biology Vol. 3, No. 2, Year 2020
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.3.2.99-104

Isolasi khamir penghasil enzim inulinase dari buah kersen (Muntingia calabura) serta pengaruh mikronutrien mangan (Mn) pada produksi enzimnya Fitri Ulfana Risky; Wijanarka Wijanarka; Sri Pujiyanto
NICHE Journal of Tropical Biology Vol. 2, No. 2, Year 2019
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Pemanfaatan khamir inulinolitik indigenous ampas tebu (bagasse) sebagai penghasil biokatalis inulinase dalam produksi gula rendah kalori Moch Ali Utomo; Tia Erfianti; Dewi Ayu Maryati; Romario Dion; Pragati Wira Anggini; Wijanarka Wijanarka; Mochamad Hadi
NICHE Journal of Tropical Biology Vol. 4, No. 2, Year 2021
Publisher : Department of Biology, Faculty of Sciences and Mathematics, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/niche.4.2.56-63

Identifikasi dan Karakterisasi Isolat 23 Ducc Serta Kemampuannya dalam Memproduksi Enzim Inulinase Wijanarka Wijanarka; K Endang; Hermin Hermin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Inulinase has a capacity to convert inulin into fructose hence it could be used in high fructose syrup production. Inulinase is not readily available in Indonesian market. This enzyme could be obtained from thermotolerant yeast found in Dahlia variabilis. Therefore this study was conducted to identify inulinolytic yeast in D. variabilis. The results showed that an inulinolytic yeast isolate 23 DUCC from D. variabilis tubers was found. The inulinolitic yeast produces inulinase (E.C.3.2.1.7). Based on the identification and determination, the isolate is known to be Kluyveromyces marxianus. The best optimation condition for this yeast was as follows: concentration of carbon source (inulin) was 0.75%, pH was 5.5 and time of fermentation was 53 hours. In such condition K. marxianus produced inulinase up to 0.6481 IU.

Produksi Pigmen Karotenoid oleh Khamir Phaffia rhodozyma yang Diperlakukan dengan Radiasi Sinar UV Sri Pujiyanto; Wijanarka Wijanarka; Endang Kusdiyantini; T. A. Lestari
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 2 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.2.146

Carotenoid pigment is an essential element in aquaculture, since it gives characteristic of color on shrimp and fish. Carotenoid pigments can be produced microbiologically using Paffia rhodozyma. Genetic improvement of the yeast, one of which can be accomplished by radiation mutation, will increase the production of carotenoid pigments. The aims of this study were to mutate P. rhodozyma using UV irradiation and to figure out pigment production by the mutant strains resulting from 30 minute-irradiation. Irradiated culture was incubated in dark condition and plated onto YMA media. Grown mutant colonies were collected in order to test for their pigment production. Pigment production was measured on the basis of extinction coefficient of 1%. The results showed that mutant strain encoded with MUV-1 produced the highest pigment at 179.96 mg/g dry weight cell, higher than the wild type (63.20 mg/g dry weight cell).

Optimasi Starter dalam Memproduksi Inulinase dan Identifikasi Khamir Inulinolitik BAN - 1 dari Umbi Dahlia (Dahlia variabilis Willd) Wijanarka Wijanarka; Endang Kusdiyantini; Hermin Hermin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 3 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.3.205

Inulinolitic yeast isolated from dahlia tuber (Dahlia variabilis Willd) of Bandungan-Ambarawa, was found to produce inulinase enzyme (E.C.3.2.1.7). Under tested fermentation condition, BAN-1 inulinase isolate showed enzymatic activity in dose dependent manner to the starter concentration. A maximum enzymatic activity of 0.582 IU was produced by adding starter concentration 10%. Based on morphological, physiological and biochamichal evaluation it was indentified that the BAN-1 isolate is Pichia sp.

Uji dan identifikasi aktivitas antioksidan isolat BAL CIN-2 hasil isolasi cincalok Ihdina Isfara Suteja; Wijanarka Wijanarka; Endang Kusdiyantini
Jurnal Penelitian Saintek Vol 27, No 1 (2022)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v1i1.44187

Penelitian ini bertujuan untuk menguji antioksidan yang dihasilkan oleh isolat BAL CIN-2 dengan metode (α,α-diphenyl-β-picrylhydrazyl) (DPPH) dan fosfomolibdenum, melakukan identifikasi senyawa antioksidan yang dihasilkan isolat BAL CIN-2 menggunakan metode TLC (Thin Layer Chromatography) dan spektrofotometer UV-Vis, serta menganalisis gugus fungsional antioksidan isolat BAL CIN-2 menggunakan metode Fourier Transform Infrared Spectroscopy (FTIR). Kultur ditumbuhkan pada media MRSA dan diamati pertumbuhannya pada media MRSB selama 72 jam, selanjutnya dianalisis dengan metode yang telah ditentukan. Hasil uji TLC untuk isolat BAL CIN-2 menghasilkan empat noda dengan nilai Rf 0,98; 0,91; 0,73; dan 0,6. Uji DPPH menunjukkan sifat antioksidan supernatan isolat BAL CIN-2 sangat lemah. Metode fosfomolibdenum menunjukkan kadar antioksidan dari supernatan isolat BAL CIN-2 sebesar 1,906 mgAAE/ml. Pengujian menggunakan spektrofotometer UV-Vis menghasilkan 4 serapan gelombang dengan absorbansi maksimum 3,943 pada panjang gelombang 290 nm. Diduga senyawa yang terkandung di dalamnya adalah senyawa flavonoid golongan flavanon atau dihidroflavonol. Supernatan isolat BAL CIN-2 diduga mengandung senyawa flavonoid dengan gugus fungsi antioksidan O-H dan C=C aromatik dari analisis menggunakan FTIR. Berdasarkan hasil tersebut dapat disimpulkan bahwa di dalam supernatan isolat BAL CIN-2 mengandung senyawa antioksidan golongan flavonoid.Test and identification of antioxidant activity of isolates LAB CIN-2 isolated from cincalokThis study aimed to test the antioxidants produced by LAB CIN-2 isolates using α,α-diphenyl-β-picrylhydrazyl (DPPH) and phosphomolybdenum methods, to identify antioxidant compounds produced by LAB CIN-2 isolates using the Thin Layer Chromatography (TLC) and UV-Vis spectrophotometer, as well as to analyse the antioxidant functional groups of LAB CIN-2 isolate using the Fourier Transform Infrared Spectroscopy (FTIR) method. The culture was grown on MRSA media and then the growth was observed on MRSB media for 72 hours, then analyzed using the specified method. TLC test results showed that LAB isolate CIN-2 produced four spots with an Rf value of 0.98; 0.91; 0.73; and 0.6. The DPPH test showed that the antioxidant properties of the supernatant isolates of LAB CIN-2 were very weak. The phosphomolybdenum method showed that the antioxidant content of the supernatant of LAB CIN-2 isolate was 1.906 mgAAE/ml. Analysis using a UV-Vis spectrophotometer produces 4 absorption waves with a maximum absorbance 3,943 at a wavelength of 290 nm. The test is suspected that the compounds contained in it are flavonoid compounds of the flavanone or dihydroflavonol group. Analysis using FTIR showed that antioxidant from supernatant of LAB isolate CIN-2 was suspected to contain flavonoid compounds with aromatic O-H and C=C functional groups. It can be concluded that supernatant from LAB CIN-2 isolates contains weak antioxidants in the form of compounds from the flavonoids group.

Analisis Total Kadar Asam Laktat Hasil Fermentasi Pare (Momordica charantia) Secara Spontan dengan Konsentrasi Garam Berbeda Putri S. Noor; Wijanarka Wijanarka
Jurnal Biologi Papua Vol 13, No 2 (2021)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Bitter melon (Momordica charantia) is a traditional medicinal plant with great potential for treating diabetes due to its ability to lower blood sugar level. The bitter taste of this plant is attributed to its high nutrient and antioxidants content. Meanwhile, one of the ways to reduce the bitterness and improve its taste is through a spontaneous fermentation process with the right salt concentration. Therefore, this study aims to analyze the effect of different salt concentrations on the total lactic acid content of spontaneous bitter melon fermentation. This study was conducted using a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments used included salt of different concentrations, namely: 3, 6, 9, and 12%. The bitter melon was fermented for 7 days and the parameter measured was the total lactic acid content through titration process. Furthermore, data were analyzed by ANOVA with 5% significance and the Duncan test was also carried out. The results showed different salt concentrations had a significant effect on lactic acid contents (P <0.05), with the highest being 0.4413% produced by 3% salt concentration treatment. These results are expected to contribute to the development of bitter melon utilization, as well as provide solutions, therefore, it becomes more effective and efficient food processing. Key words: bitter melon; fermentation; lactic acid; salt; spontaneous.

Kemampuan Prospektif Fusan FP4 dalam Memproduksi Inulinase dan Profil Pertumbuhannya Wijanarka Wijanarka; Ika Anggraini
Jurnal Biologi Papua Vol 9, No 1 (2017)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Fusan FP4 is one type fused materials derived from intraspecific fusion of Pichia manshurca. The fused materials have superior capabilities compared with other materials, especially in producing inulinase enzyme (E.C. 3.2.1.7). The aims of the study are to determine the production of the enzyme inulinase of fusan FP4 and the growth profile formed. Growth can be detected by the addition of size and cell division. Growth measurements of fusan FP4 was carried out for 24 hours and observation and sampling was done every 6 hours for 24 hours. Observations of growth are conducted qualitatively by using a spectrophotometer, while the production of inulinase done using DNS method. The results of this study showed that the highest inulinase production was 1.948 U/ml and occurred at log phase with 6 hours of incubation time (t6) to 12 hours (t12). Key words: inulinase, Fusan FP4, log phase, P. manshurca.

Co-Authors Agung Suprihadi Aini, Riris Nur Anggoro, Naufal Sebastian Anto Budiharjo Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Arina Tri Lunggani Asy'ari, Mukhammad Ayuningtyas, Annisa Nur Azzahra, Meilidya Falkhiya Chatarina Umbul Wahyuni Dewi Ayu Maryati Doktorasaintifika, Heradita Kaniaazzahra Dyah Wulandari Endang Kusdiyantini Endang Kusdiyantini Endang Kusdiyantini Endang Sutariningsih Endang Sutariningsih ENDANG SUTARININGSIH SOETARTO Endang Sutariningsih Soetarto Endang Sutariningsih Soetarto Fathika Fitrania Fauzaan, Muhammad Faishal Fitri Ulfana Risky Fransenda, Auxensius Rexer Ginting, Ledy HARTAJANIE, LAKSMI Herida, Azalia Puspa Hermin Hermin Hermin Pancasakti Kusumaningrum Ihdina Isfara Suteja Ika Anggraini Indra Bakti Sangalang Inggrit Amedia, Inggrit K Endang Kumala Dewi Kumala Dewi Kumala Dewi Kumala Dewi Lailatul Mubarokhah Laily Kurniawati Lindayani, Lindayani Lynda - Sutrisna Maria Sarah Fadillah Maulana, Anand Reyna Maulida Aglinia Mauritz Nicolaas Wattimena Mawarni, Risa Arum MG Isworo Rukmi Moch Ali Utomo Mochamad Hadi Muhammad Nur Muhammad Zainuri NANIK RAHMANI Nomeritae Nomeritae Noor Hamidah Nurhayati Nurhayati Pragati Wira Anggini Pratama Jujur Wibawa Putri S. Noor Rahmasari, Dianti Rahmasari, Dianti Rahmawati Dewi Rejeki Siti Ferniah Romario Dion Rudi Waluyo salamah salamah Sarjana Parman Sarsa A.N Shabrina, Jauhara Siti Maisaroh Siti Nur Jannah Sri Pujiyanto Sulis Setyowati, Sulis Susiana Purwantisari T. A. Lestari Tia Erfianti Tony Hadibarata, Tony Wahyuningsih, Candra YOPI YOPI Yusuf, Hadistiyani

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