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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari Jurnal Bios Logos Biosaintifika: Journal of Biology & Biology Education Jurnal Widya Biologi SIMBIOSIS
Ni Nyoman Wirasiti
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health

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JENIS POHON, PEMANFAATAN SERTA KEPERCAYAAN MASYARAKAT DESA PELIATAN, KECAMATAN UBUD, GIANYAR-BALI I W. Suarna; A. A. G. R. Dalem; N. N. Wirasiti
Bumi Lestari Journal of Environment Vol 6 No 1
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A study on identification of trees species, their use as well as what the communitybelieve about them held in Peliatan Village, Ubud District, Gianyar Regency (Bali) onFebruary, 2005. The study was carried out by taking a walk along the roads or trails whichwere assessed for their potentials as tracking trails in this village while taking notes on thetree species observed. The trees then were determined their status, their use as well as whatthe community believe about them. Results of this study show that there were 148 kinds oftrees which have been identified on this village. From the use perspective, it was revealedthat these trees could give a wide range of benefits for the community of Peliatan as well asBali in general. Their use for decorations, provides shady spots, as well as uncultivated treeswere the highest in their proportion (22%). In addition, in this village they were alsoidentified 18% of trees which believed to provide a possitive or negative impacts to humans.Most of them (73%) were believed to be related to positive impacts to humans.
JENIS DAN MANFAAT FLORA PULAU DERAWAN KABUPATEN BARAU PROVINSI KALIMANTAN TIMUR Ni Nyoman Darsini; I .G. A. Sugi Wahyuni; A.A.K. Darmadi; Ni Nyoman Wirasiti
Bumi Lestari Journal of Environment Vol 8 No 1 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A study on identication of plants species available on Derawan Island, Berau Regency, East Kalimantan has been conducted based on exploration survey around the island between 2002-2005. The plants whicht have observed were then listed on a table, their scientific names wrre recorded, their status (whether being protected or not) were determined, and the benefits or use of the plants were also listed. From this study it can be found 45 species of 30 families. They may bring benefits for the locals as vegetables, source of medicine, providing shady spots, or decorative plants.
JENIS-JENIS DAN KARAKTERISTIK BURUNG YANG DITEMUKAN DI KAWASAN BEDUGUL DAN SEKITARNYA N. N. Wirasiti; N. M. Rai Suarni; A. A. G. Raka Dalem
Bumi Lestari Journal of Environment Vol 5 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A study on birds (species and their characteristics) was carried out in Bedugul(Bali) between November and December 2004. Observation was made along transectlines which are set prior to the study. Species of birds observed were identified andrecorded. In addition, their distributions, status, source of food, reproductive seasons, aswell as migration patterns (whether migrant species or not) were determined. Thisstandard sampling strategy was also supported by casual observation and interview.Results of this study showed that Bedugul and surrounding area have a significantrole in conservation of Bali birds, because at least 56 species of birds have beenidentified in this area, which comprises of 18% of Bali birds. Conservation priorityshould be put on 8 species (14%) of the birds which are protected by law, as well as on 7% of endemic species (for Java and Bali). In addition, conservation effort should be alsogiven priority to 9% of birds that considered to be rare and potentially that be locallyextict. Habitat conservation should becoming focus of attention since it determines theavailability of food sources for those birds. Conservation on forest, plantation as well asshrubs should become focus of attention since these become habitats of more than a half(57%) of birds available in this area.
SUBSTITUTION OF COMMERCIAL FEED WITH MORINGA LEAF MEAL TO IMPROVE THE PERFORMANCE OF MALE RABBIT N.M.R. Suarni; N.G.A.M. Ermayanti; N.N. Wirasiti
JURNAL WIDYA BIOLOGI Vol 12 No 01 (2021): Widya Biologi
Publisher : UNHI Press

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Abstract

The aims of this study were to find out the effects of moringa leaf meal substitution in commercial feed on the performance of male rabbits (bucks). Twenty four four months old male rabbits with an average live weight of 1200g were used in this study. There were four treatments in this study including 0% (K0) as control and 15% (K1), 30% (K2), 45% (K3) substitution of moringa leaf meal in commercial feed and each treatment consisted of six replicates. The treatments were carried out for two months. The variables measured were including body weight, feed consumption, feed conversion. The results showed that there were significant differences (P <0.05) performance between all treatments (K1, K2, K3) and the control (K0). Substitution of moringa leaf meal up to 45% in commercial feed increase performance of male rabbit. It was concluded that the moringa leaf meal might be substituted in commercial feed up to 45 % to improve the performance of male rabbits (bucks). To get maximum performance of male rabbit rabbits it should be better substituted the commercial feed with moringa leaf meal is 35%.
Morfologi Dan Rasio Spermatozoa X : Y Mencit (Mus Musculus) Yang Diberi Ekstrak Buah Juwet (Syzygium Cumini) Setelah Terpapar Asap Rokok Yoseph Raymond Irawan; NI Nyoman Wirasiti; Ni Made Rai Suarni; A.A.S.A Sukmaningsih
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p10

Abstract

Buah Juwet merupakan buah dengan antioksidan tinggi yang dapat meningkatkan kemampuan reproduksi. Penelitian ini bertujuan untuk melihat pengaruh ekstrak buah juwet terhadap morfologi dan rasio spermatozoa X dan Y setelah terpapar asap rokok. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan yaitu K-, K+ (pemberian 1 batang rokok komersil/hari), P1 (diberi ekstrak buah juwet 1,8mg/g BB dan satu batang rokok komersil/hari), P2 (diberi ekstrak buah juwet 1,8mg/g BB) dan P3 (pemberian satu batang rokok dengan filter bubuk ekstrak buah juwet/hari) dan setiap perlakuan terdapat 4 kali ulangan. Parameter yang diamati dalam penelitian ini adalah spermatozoa dengan morfologi abnormal dan rasio X : Y spermatozoa. Hasil penelitian menunjukkan bahwa terdapat perbedaan yang signifikan pada persentase spermatozoa abnormal antara kelompok kontrol dan perlakuan. Pada penelitian ini ekstrak buah juwet tidak berpengaruh secara signifikan terhadap rasio spermatozoa X dan Y. Dapat disimpulkan bahwa ekstrak buah juwet mampu menurunkan resiko munculnya kelainan morfologi pada spermatozoa setelah terpapar asap rokok. Namun ketiga metode tersebut belum mampu secara efektif mempengaruhi rasio spermatozoa X dan Y setelah terpapar asap rokok. Kata kunci: ekstrak juwet, mencit, radikal bebas, rasio spermatozoa, rokok.
Optimasi digesti enzim restriksi untuk deteksi mutasi daerah D-loop DNA mitokondria dengan metode PCR-RFLP Ni Putu Senshi Septiasari; I Ketut Junitha; Ni Nyoman Wirasiti
Jurnal Biologi Udayana Vol 27 No 1 (2023): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2023.v27.i01.p07

Abstract

Metode PCR-RFLP merupakan salah satu metode untuk deteksi mutasi pada daerah D-loop DNA mitokondria. Metode ini menggunakan enzim restriksi untuk dapat memotong DNA mitokondria dan menghasilkan ukuran fragmen DNA yang berbeda-beda. Enzim restriksi memerlukan kondisi yang optimal untuk melakukan pemotongan DNA. Penelitian ini bertujuan untuk mengetahui kondisi optimal enzim restriksi agar dapat melakukan digesti pada daerah D-loop DNA mitokondria. Optimasi dilakukan dengan membuat dua kombinasi formula digesti (formula 1 dan 2) dan empat macam waktu digesti (2 jam, 4 jam, 6 jam dan overnight). Hasil penelitian menunjukkan optimasi dari lima macam enzim restriksi (HaeIII (BsuRI), HindIII, HinfI, MboI dan HpyP31 (DdeI)) didapatkan bahwa ada perbedaan formula dan waktu digesti tergantung dari jenis enzim. Enzim HaeIII(BsuRI), HinfI dan MboI menunjukkan formula 2 merupakan formula optimal, sedangkan enzim HpyP31 (DdeI) formula 1 merupakan formula yang optimal. Waktu digesti 2 jam menunjukkan hasil optimal pada Enzim HaeIII(BsuRI), MboI dan HpyP31 (DdeI), sedangkan enzim HinfI waktu digesti optimal adalah 4 jam. Enzim HindIII tidak mendapatkan hasil potongan fragmen DNA setelah digesti, maka enzim HindIII tidak memiliki situs pemotongan pada daerah D-loop DNA mitokondria.
Optimasi PCR dengan Penanda Daerah D-loop DNA Mitokondria untuk Metode Tes DNA Ni Putu Senshi Septiasari; I Ketut Junitha; Ni Nyoman Wirasiti
Indonesian Journal of Legal and Forensic Sciences (IJLFS) Vol 12 No 2 (2022): Indonesian Journal of Legal and Forensic Sciences
Publisher : Penerbit, sejak 2012 : Asosiasi Ilmu Forensik Indonesia dan UPT Lab. Forensik Sain dan Kriminilogi - Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2022.v12.i02.p03

Abstract

DNA testing is a molecular personal identification that can distinguish between individuals. One of the DNA markers used for PCR tests is mitochondrial DNA markers. Mitochondrial DNA is reported to have a polymorphic locus in the mitochondrial D-loop region. This study aims to determine the appropriate method to amplify mitochondrial DNA in the D-loop region for the DNA testing. A sample of 65 people was taken by purposive sampling method. Samples were extracted with phenol-chloroform and PCR with 2 types of PCR formulas, is formulas A and formulas B and the annealing temperatures used were 48oC, 52oC dan 56oC. The results showed that the optimal primary concentration for successful amplification was a Formulation B with primer concentration of 0,4 µM and condition variation II with annealing temperature of 52oC. A total of 62 Balinese people were successfully amplified with an amplicon length of 860 bp. Samples that are not successfully amplified are affected by the number of collected epithelial cells, the success of the DNA extraction process and null allele. So, from these results it can be concluded that the optimal conditions of DNA marker with a primer concentration of 0,4 µM and annealing temperature conditions of 52oC succeeded in amplifying the D-loop region of mitochondrial DNA.
FORMULASI EKSTRAK ETANOL DAUN MALAKA (Phylanthus emblica) DALAM SEDIAAN MASKER GEL PEEL-OFF Agustina Bayo Inggirik Mogan; Ni Luh Arpiwi; Ni Nyoman Wirasiti
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p01

Abstract

The use of facial masks is useful for softening the skin, nourishing and opening clogged pores, helping prevent premature aging, and reducing the appearance of fine lines and wrinkles. Plants that can be used as active ingredients are malaka, or amla (Phyllanthus emblica L.), a natural source of antioxidants. This study aims to determine the content of phytochemical compounds in the ethanol extract of Malaka leaves, to analyze the antioxidant activity and IC50 of the ethanol extract of Malaka leaves, to determine the quality of the peel-off gel mask with the addition of the concentration of the ethanol extract of Malaka leaves and to find out which formulation is preferred by Probandus. The research design used a completely randomized design (CRD) using Malaka leaf extract with concentrations F0(0%), F1(1%), F2(2%) and F3(3%). The phytochemical content test of Malaka leaf extract showed the presence of flavonoids (0.467%) and tannins (10.97%). The antioxidant activity of the ethanol extract of Malaka leaves was 1.3314% and IC50 was 30 ppm. The organoleptic test results for all preparations did not change during 5 weeks of storage, pH 5.37-5.54, the viscosity of the mask preparation was 50000-126000 cps, the peel-off gel mask preparation was homogeneous and did not . Statistical analysis of the
HUBUNGAN ANTARA RIWAYAT KETURUNAN DAN FAKTOR RISIKO TERHADAP KEJADIAN ASMA: THE RELATIONSHIP BETWEEN HEREDITARY HISTORY AND RISK FACTORS FOR ASTHMA Prita Jaya, Made Ayu Mutiara; Junitha, I Ketut; Wirasiti, Ni Nyoman
SIMBIOSIS Simbiosis Volume 13 No. 2 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i02.p03

Abstract

ABSTRACT Asthma is a disease caused by chronic inflammation of the respiratory tract, which causes shortness of breath and difficulty breathing. This disease arises due to genetic factors or heredity. Genetic diseases may not be inherited again in the next generation, but can recur due to several risk factors that trigger asthma. This study aims to analyze the relationship between hereditary history and risk factors on the incidence of asthma. Sampling was carried out using a purposive sampling technique, data collection was carried out by measuring the variables studied using questionnaires and interviews, and data analysis was carried out using correspondence analysis. The results of the study show that not all asthma patients have a family history of asthma, and non-genetic risk factors that cause asthma recurrence consist of age, gender, body mass index (BMI), home location, smoking habits, and environmental factors consisting of fatigue, cold weather, dust allergies, mental influences, air pollution, animal dander allergies, and food allergies. Keywords: Allergies, asthma, genetics, gender, shortness of breath, age
Co-Authors A. A. G. R. Dalem A.A.S. A. Sukmaningsih AGUNG EKO CAHYA KUSUMA PUTRA Agustina Bayo Inggirik Mogan Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Andi Ratu Bulqis I GUSTI AYU MANIK ERMAYANTI I Gusti Ayu Manik Widhyastini I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Junitha I KETUT JUNITHA I Ketut Junitha I KETUT MUKSIN I W. Suarna I.G.A.S. Wahyuni Ida Bagus Bajing Agastya IRIANI SETYAWATI Kezia Artanauli Purba LUH Puru ESWARYANTI KUSUMA YUNI Luh Putu Wrasiati MADE PHARMAWATI N.G.A.M. Ermayanti Ni Luh Arpiwi Ni Made Rai Suarni NI NYOMAN DARSINI Ni Putu Putri Wulandari Ni Putu Sukma Widyantari Ni Wayan Sudatri Prita Jaya, Made Ayu Mutiara Retno Kawuri Sally Nabila Rizal Septiasari, Ni Putu Senshi Tyara Tyara Yoseph Raymond Irawan