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All Journal INDONESIAN JOURNAL OF LEGAL AND FORENSIC SCIENCES METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Biologi Udayana Bumi Lestari Jurnal Bios Logos SIMBIOSIS Biosaintifika: Journal of Biology & Biology Education Jurnal Widya Biologi

Ni Nyoman Wirasiti

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 418862

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health

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JENIS POHON, PEMANFAATAN SERTA KEPERCAYAAN MASYARAKAT DESA PELIATAN, KECAMATAN UBUD, GIANYAR-BALI I W. Suarna; A. A. G. R. Dalem; N. N. Wirasiti
Bumi Lestari Journal of Environment Vol 6 No 1
Publisher : Environmental Research Center (PPLH) of Udayana University

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A study on identification of trees species, their use as well as what the communitybelieve about them held in Peliatan Village, Ubud District, Gianyar Regency (Bali) onFebruary, 2005. The study was carried out by taking a walk along the roads or trails whichwere assessed for their potentials as tracking trails in this village while taking notes on thetree species observed. The trees then were determined their status, their use as well as whatthe community believe about them. Results of this study show that there were 148 kinds oftrees which have been identified on this village. From the use perspective, it was revealedthat these trees could give a wide range of benefits for the community of Peliatan as well asBali in general. Their use for decorations, provides shady spots, as well as uncultivated treeswere the highest in their proportion (22%). In addition, in this village they were alsoidentified 18% of trees which believed to provide a possitive or negative impacts to humans.Most of them (73%) were believed to be related to positive impacts to humans.

JENIS DAN MANFAAT FLORA PULAU DERAWAN KABUPATEN BARAU PROVINSI KALIMANTAN TIMUR Ni Nyoman Darsini; I .G. A. Sugi Wahyuni; A.A.K. Darmadi; Ni Nyoman Wirasiti
Bumi Lestari Journal of Environment Vol 8 No 1 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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A study on identication of plants species available on Derawan Island, Berau Regency, East Kalimantan has been conducted based on exploration survey around the island between 2002-2005. The plants whicht have observed were then listed on a table, their scientific names wrre recorded, their status (whether being protected or not) were determined, and the benefits or use of the plants were also listed. From this study it can be found 45 species of 30 families. They may bring benefits for the locals as vegetables, source of medicine, providing shady spots, or decorative plants.

JENIS-JENIS DAN KARAKTERISTIK BURUNG YANG DITEMUKAN DI KAWASAN BEDUGUL DAN SEKITARNYA N. N. Wirasiti; N. M. Rai Suarni; A. A. G. Raka Dalem
Bumi Lestari Journal of Environment Vol 5 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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A study on birds (species and their characteristics) was carried out in Bedugul(Bali) between November and December 2004. Observation was made along transectlines which are set prior to the study. Species of birds observed were identified andrecorded. In addition, their distributions, status, source of food, reproductive seasons, aswell as migration patterns (whether migrant species or not) were determined. Thisstandard sampling strategy was also supported by casual observation and interview.Results of this study showed that Bedugul and surrounding area have a significantrole in conservation of Bali birds, because at least 56 species of birds have beenidentified in this area, which comprises of 18% of Bali birds. Conservation priorityshould be put on 8 species (14%) of the birds which are protected by law, as well as on 7% of endemic species (for Java and Bali). In addition, conservation effort should be alsogiven priority to 9% of birds that considered to be rare and potentially that be locallyextict. Habitat conservation should becoming focus of attention since it determines theavailability of food sources for those birds. Conservation on forest, plantation as well asshrubs should become focus of attention since these become habitats of more than a half(57%) of birds available in this area.

DIFFERENCE IN SPERM QUALITY IN PATIENTS WITH TYPE 1 AND 2 DIABETES MELLITUS IN RSUD. LAMADUKELLENG, SENGKANG, SULAWESI SELATAN Andi Ratu Bulqis; Ni Gusti Ayu Manik Ermayanti; Nyoman Wirasiti
SIMBIOSIS Vol 8 No 1 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Diabetes mellitus merupakan gangguan metabolisme yang disebabkan kurangnya hormon insulin dan menyebabkan kelainan metabolisme karbohidrat, protein dan lemak. Diabetes mellitus akan mempengaruhi beberapa kinerja organ dalam tubuh manusia. Salah satunya yaitu berpengaruh terhadap produksi kualitas sperma pada tubulus seminiferus. Tujuan dilakukan penelitian ini adalah untuk mengetahui perbedaan kualitas sperma pada pasien penderita diabetes mellitus tipe 1 dan 2 di Rumah Sakit Umum Daerah Lamadukelleng, Sengkang. Sampel diambil dari 30 laki-laki yang bersedia sebagai probandus sesuai dengan kriteria yang telah ditentukan yaitu penderita DM tipe 1 dan 2, berumur 20-45 tahun. Kemudian dimasukkan kelompok kontrol, DM 1 dan DM 2 menggunakan metode Komperatif dengan jumlah probandus masing-masing kelompok sebanyak 10 orang. Metode analisa kualitas sperma yang dilakukan di Laboratorium Andrologi, Rumah Sakit Umum Daerah Lamadukelleng, Sengkang adalah makler chamber, kemudian dilakukan pemeriksaan analisa kualitas sperma seperti pada umumnya. Data yang didapat dari hasil penelitian ini berupa data kualitatif dan kuantitatif. Data kualitatif meliputi morfologi, disajikan dalam bentuk gambar. Kemudian data kuantitatif meliputi, penghitungan konsentrasi, motilitas dan morfologi disajikan dalam bentuk tabulasi data. Setelah itu data kuantitatif dibaca dalam metode penghitungan statistik menggunakan SPSS Versi 24, dengan One Way Anova untuk melihat perbedaan antara sampel DM tipe 1 dan 2 yang berbeda signifikan (p < 0,05). Kesimpulan penelitian ini yaitu terdapat perbedaan konsentrasi, motilitas dan morfologi pada pasien penderita DM tipe 1 dan 2.

VARIASI POLA SIDIK JARI SOROH BRAHMANA SIWA DI BALI Ida Bagus Bajing Agastya; I Ketut Junitha; Ni Nyoman Wirasiti
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

ABSTACTResearch was conducted to determine the patterns of Brahmana Shiva fingerprint in Bali. The fingerprint samplesas many as 240 people, which consist of 120 men and 120 women from four generation of Brahmana Shiva in Bali. Thefingerprints were collected using a fingerprint pad then the data were analyzed using Chi–Square test, Student-t test,Dankmerijer index and Furuhata index. The result of fingerprints examination shown that there were four fingerprintspattern i.e. arch (A), loop ulnar (LU), loop radial (LR), and whorl (W) from four sub clan (Brahmana Manuaba, Kemenuh,Keniten, Mas), with different frequencies and anequal distribution from each fingers and both hands. The arch (A) patternin clan Brahmana Manuaba and Kemenuh have the same 0.83 % frequencies, while the frequencies of Keniten 1.17 % andMas 1.67 %. The frequencies of Loop ulnar (LU) pattern from clan Brahmana Mas were 42.66 %, Manuaba 36 %, Keniten39 %, and Kemenuh 34.33 %. The frequency of loop radial (LR) pattern from clan Brahmana Manuaba were 42.17 %,Keniten 41.83 %, Mas 37.83 %, and Kemenuh 29.51 %. The frequencies of whorl (W) pattern from sub clan BrahmanaKemenuh, Manuaba, Keniten, and Mas were 35.33 %, 22 %, 18 %, and 17.83 %, respectively. The result show asignificant differences between sub clan Mas and Kemenuh using Dankmerijer Index (DI). The result show a significantdifferences between sub clan Kemenuh with Mas, Keniten and Manuaba using Furuhata Index (FI). Total Ridge Countbetween men and women of Brahmana Shiva offspring showed unsignificantly diferences at level 5%. However, there wasa significant difference of Total Ridge Count from sub clan Manuaba with Mas, Kemenuh, and Keniten.Keywords : fingerprints, arch, loop, whorl, clan brahmana shiva.

SUBSTITUTION OF COMMERCIAL FEED WITH MORINGA LEAF MEAL TO IMPROVE THE PERFORMANCE OF MALE RABBIT N.M.R. Suarni; N.G.A.M. Ermayanti; N.N. Wirasiti
JURNAL WIDYA BIOLOGI Vol 12 No 01 (2021): Widya Biologi
Publisher : UNHI Press

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The aims of this study were to find out the effects of moringa leaf meal substitution in commercial feed on the performance of male rabbits (bucks). Twenty four four months old male rabbits with an average live weight of 1200g were used in this study. There were four treatments in this study including 0% (K0) as control and 15% (K1), 30% (K2), 45% (K3) substitution of moringa leaf meal in commercial feed and each treatment consisted of six replicates. The treatments were carried out for two months. The variables measured were including body weight, feed consumption, feed conversion. The results showed that there were significant differences (P <0.05) performance between all treatments (K1, K2, K3) and the control (K0). Substitution of moringa leaf meal up to 45% in commercial feed increase performance of male rabbit. It was concluded that the moringa leaf meal might be substituted in commercial feed up to 45 % to improve the performance of male rabbits (bucks). To get maximum performance of male rabbit rabbits it should be better substituted the commercial feed with moringa leaf meal is 35%.

KUANTIFIKASI DNA PADA MAHASISWA PEROKOK DAN BUKAN PEROKOK DI UNIVERSITAS NEGERI MEDAN KECAMATAN MEDAN TEMBUNG KOTA MEDAN PROVINSI SUMATERA UTARA Kezia Artanauli Purba; I Ketut Junitha; Ni Nyoman Wirasiti
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Individual identification is very important in forensics. DNA can be obtained from all parts of the body with the same profile in everyone. Oral mucosal epithelium is one of the sources of DNA that is often used to examine individuals because it is taken using a harmless swab method. Smoke is one of the behaviors that are mostly done by young people or teenagers. Cigarette smoke affects the cells of the oral mucosa because it is a free radical. Free radicals are reactive oxygen compounds which are compounds with unpaired electrons. Deoxyribonucleic acid (DNA) is a nucleic acid polymer that is systematically arranged and is a carrier of genetic information that is passed on to offspring. This study aims to determine the quality and quantity of DNA in non-smoking and smoking students at the State University of Medan, Medan Tembung District, Medan City, North Sumatra Province. Samples were taken by swab method, namely mucosal epithelium from 60 probands consisting of 30 smoker proband and 30 non- smoking proband aged 18-22. The cheek in the probandus is swabbed from the back to the front in one direction. In this study, DNA extraction was carried out using a chelex solution which aims to separate DNA from protein, test the quantity of DNA with a spectrophotometer and test the quality of DNA with agarose gel. The results showed that the average DNA quantity in the oral mucosal samples was 1.96 ng/µL in the smokers proband and 6.92 ng/µL in the non-smoker proband and. The results of the quality test using electrophoresis on agarose gel showed that smoking students had thin bands of fluorescence and in some samples no bands of fluorescence at all. Meanwhile, in the probandus of non-smoker students, there were several samples that showed thin bands of luminescence and stains.

THE QUALITY OF SPERM ON ADOLESCENT SMOKERS AT UDAYANA UNIVERSITY Sally Nabila Rizal; A.A.S. A. Sukmaningsih; Ni Nyoman Wirasiti
SIMBIOSIS Vol 11 No 1 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i01.p05

Smoking is a familiar behavior for male adolescents. The reason is because cigarette contains toxins which can increase our Reactive Oxygen Species (ROS) and causes tissue oxidative stress, thereby disrupting the quality of spermatozoa. The purpose of this study is to determine the quality of sperm on adolescent smokers in Udayana University. The samples were taken from 30 male adolescents with an age range of 18 – 24 years old. This included 15 smokers and 15 non-smokers as the control group. The macroscopic observations included color, smell, liquefaction, volume, consistency and sperm pH – while the macroscopic observations included concentration, morphology, motility, viability, and membrane integrity. This study used a comparative method. The qualitative data included the color and smell of spermatozoa, while the quantitative data included concentration, morphology, motility, liquefaction, volume, consistency, viability, membrane integrity and pH – all which were presented in the form of mean and standard deviation. The quantitative data were analyzed statisticall with t test using SPSS application. The results obtained from this study showed that liquefaction, volume, consistency, motility, viability, concentration, and morphology of spermatozoa in smokers had a decrease compared to non-smokers (p < 0.01). Meanwhile, color, smell, pH, and integrity of spermatozoa membranes showed no significant difference between smokers and non-smokers (p > 0.01).

Morfologi Dan Rasio Spermatozoa X : Y Mencit (Mus Musculus) Yang Diberi Ekstrak Buah Juwet (Syzygium Cumini) Setelah Terpapar Asap Rokok Yoseph Raymond Irawan; NI Nyoman Wirasiti; Ni Made Rai Suarni; A.A.S.A Sukmaningsih
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p10

Buah Juwet merupakan buah dengan antioksidan tinggi yang dapat meningkatkan kemampuan reproduksi. Penelitian ini bertujuan untuk melihat pengaruh ekstrak buah juwet terhadap morfologi dan rasio spermatozoa X dan Y setelah terpapar asap rokok. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan yaitu K-, K+ (pemberian 1 batang rokok komersil/hari), P1 (diberi ekstrak buah juwet 1,8mg/g BB dan satu batang rokok komersil/hari), P2 (diberi ekstrak buah juwet 1,8mg/g BB) dan P3 (pemberian satu batang rokok dengan filter bubuk ekstrak buah juwet/hari) dan setiap perlakuan terdapat 4 kali ulangan. Parameter yang diamati dalam penelitian ini adalah spermatozoa dengan morfologi abnormal dan rasio X : Y spermatozoa. Hasil penelitian menunjukkan bahwa terdapat perbedaan yang signifikan pada persentase spermatozoa abnormal antara kelompok kontrol dan perlakuan. Pada penelitian ini ekstrak buah juwet tidak berpengaruh secara signifikan terhadap rasio spermatozoa X dan Y. Dapat disimpulkan bahwa ekstrak buah juwet mampu menurunkan resiko munculnya kelainan morfologi pada spermatozoa setelah terpapar asap rokok. Namun ketiga metode tersebut belum mampu secara efektif mempengaruhi rasio spermatozoa X dan Y setelah terpapar asap rokok. Kata kunci: ekstrak juwet, mencit, radikal bebas, rasio spermatozoa, rokok.

Optimasi digesti enzim restriksi untuk deteksi mutasi daerah D-loop DNA mitokondria dengan metode PCR-RFLP Ni Putu Senshi Septiasari; I Ketut Junitha; Ni Nyoman Wirasiti
Jurnal Biologi Udayana Vol 27 No 1 (2023): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2023.v27.i01.p07

Metode PCR-RFLP merupakan salah satu metode untuk deteksi mutasi pada daerah D-loop DNA mitokondria. Metode ini menggunakan enzim restriksi untuk dapat memotong DNA mitokondria dan menghasilkan ukuran fragmen DNA yang berbeda-beda. Enzim restriksi memerlukan kondisi yang optimal untuk melakukan pemotongan DNA. Penelitian ini bertujuan untuk mengetahui kondisi optimal enzim restriksi agar dapat melakukan digesti pada daerah D-loop DNA mitokondria. Optimasi dilakukan dengan membuat dua kombinasi formula digesti (formula 1 dan 2) dan empat macam waktu digesti (2 jam, 4 jam, 6 jam dan overnight). Hasil penelitian menunjukkan optimasi dari lima macam enzim restriksi (HaeIII (BsuRI), HindIII, HinfI, MboI dan HpyP31 (DdeI)) didapatkan bahwa ada perbedaan formula dan waktu digesti tergantung dari jenis enzim. Enzim HaeIII(BsuRI), HinfI dan MboI menunjukkan formula 2 merupakan formula optimal, sedangkan enzim HpyP31 (DdeI) formula 1 merupakan formula yang optimal. Waktu digesti 2 jam menunjukkan hasil optimal pada Enzim HaeIII(BsuRI), MboI dan HpyP31 (DdeI), sedangkan enzim HinfI waktu digesti optimal adalah 4 jam. Enzim HindIII tidak mendapatkan hasil potongan fragmen DNA setelah digesti, maka enzim HindIII tidak memiliki situs pemotongan pada daerah D-loop DNA mitokondria.

Co-Authors A. A. G. R. Dalem A.A.S. A. Sukmaningsih AGUNG EKO CAHYA KUSUMA PUTRA Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Andi Ratu Bulqis I GUSTI AYU MANIK ERMAYANTI I Gusti Ayu Manik Widhyastini I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Junitha I Ketut Junitha I KETUT MUKSIN I W. Suarna I.G.A.S. Wahyuni Ida Bagus Bajing Agastya IRIANI SETYAWATI Kezia Artanauli Purba LUH Puru ESWARYANTI KUSUMA YUNI Luh Putu Wrasiati MADE PHARMAWATI N.G.A.M. Ermayanti Ni Made Rai Suarni NI NYOMAN DARSINI Ni Putu Putri Wulandari Ni Putu Sukma Widyantari Ni Wayan Sudatri Retno Kawuri Sally Nabila Rizal Septiasari, Ni Putu Senshi Tyara Tyara Yoseph Raymond Irawan

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