Bambang Wirjatmadi
Department Of Nutrition, Faculty Of Public Health, Universitas Airlangga, Surabaya, Indonesia ; Department Of Clinical Nutrition, Airlangga University Hospital, Surabaya, Indonesia & Department Of Clinical Nutrition, PHC Hospital, Surabaya, Indonesia

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Kadar radikal superoksid (O2-), nitric oxide (NO) dan asupan lemak pada pasien hipertensi dan tidak hipertensi Astutik, Pudji; Adriani, Merryana; Wirjatmadi, Bambang
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 3, No 1 (2014)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.1 KB) | DOI: 10.14710/jgi.3.1.90-95

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Background: Hypertension closely related to impaired endothelial dependent vasodilation due to a decline in theavailability of nitric oksid (NO) and high fat intake, decreased Nitric Oksid caused due to increased levels ofsuperoksid.Objective: to analyze the differences in the levels of Superoksid Radical (O2-), Nitric oxide (N0) and fat intake inpatients with hypertension and not hypertension .Methods: this research is cross sectional, research amount sampelnya taken at random. 24 respondent patientsconsisting of 12 cases control, and 12 the age of 65 40- tahun.pengambilan samples conducted in outpatient installationregional general hospital Sidoarjo. Done bseline superoksid levels, nitric oxide and recall fat intake.Results: the average rate of Superoksid respondents not hypertension 0.250± 0, 034U/mL, 0,314± 056U/mLhypertension, Average levels of Nitric oksid respondents not hypertension 116,083± 46,962μ M, of which hypertension72,333± 40,51μ M, average consumption of fat respondents not hypertension 41,65± 8, 32gram, 51,52± 6,74gramhypertension . Based on the test results the difference against Superoksid levels of both research groups using a t-testtwo samples are free retrieved the value p < 0,05 (p = 0,004), NO content retrieved the value p < 0,05 (p = 0,023), fatintake obtained the value of p < 0,05 (p = 0,004) which means that there is a meaningful difference in the levels ofSuperoksid, Nitric oxide and the level of fat intake among respondents with no hypertension and hypertension.Conclusion: there is a radical difference in the levels of Superoksid, NO (Nitric Oxide) and fat intake in patients withhypertension and not hypertension.
Hepatoprotective Effect of Mangosteen Peel Extract on Borax-Induced Male Rats Yunita Satya Pratiwi; Bambang Wirjatmadi; Mangestuti Agil; Merryana Adriyani; Supriyadi Supriyadi
International Journal of Public Health Science (IJPHS) Vol 5, No 3: September 2016
Publisher : Intelektual Pustaka Media Utama

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.05 KB) | DOI: 10.11591/ijphs.v5i3.4798

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The aim of this study was to determine the hepatoprotective effect of mangosteen peel extract (MPE) on borax-induced male rats. Hepatoprotective effect is represented by the variables of liver weight, SGOT (Serum Glutamic oxaloacetic transaminase) and SGPT (Serum Glutamic Pyruvate Transaminase). The true experimental study used randomized separated pretest-posttest control group design. MPE was made with 50% ethanol and 0.5% carboxymethyl cellulose-sodium (CMC-Na) stabilizer solutions. Studied groups included three control groups (positive, negative pretest and posttest) and three experimental groups (MPE dose of 200 mg/kg, 400 mg/kg and 600 mg/kg rats body weight (BW)). Samples consisted 36 male rats. The length of intervention was 16 days by providing borax, and then MPE was started on day 3 (in 3 experimental groups). Results showed that up to 16th day the SGOT tended to decrease (α <0.05), while the SGPT levels was stable, and so was the liver weight. Thus, up to day 16 the MPE was generally able to provide hepatoprotective effects mainly based on the variable SGOT in doses of 200 and 400 mg/kg. It has been proved that liver function of the rats was still relatively normal. This is in relations to the antioxidant potential of the MPE polyphenols.
Heart Disease in Indonesia in 2018: An Ecological Analysis Haqqelni Nur Rosyidah; Bambang Wirjatmadi; Bambang Purwanto
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.16316

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Broccoli Effects on Hif-1 A in Spermatozoa Cells of Ratsthat Induces Psychological Stress Ahmad Nur Huda; Bambang Wirjatmadi; Bambang Purwanto
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.16334

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Broccoli Effects on HIF-1 in Heart Endothelial Cells of Rats that Induces Psychological Stress Haqqelni Nur Rosyidah; Bambang Wirjatmadi; Bambang Purwanto
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.16341

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The Role of Addition of Vitamin C in Iron Supplementation on Ferritin Serum Levels in Anemia Adolescent Females Nanik Dwi Astuti; Bambang Wirjatmadi; Merryana Adriani
Health Notions Vol 2, No 3 (2018): March
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.691 KB) | DOI: 10.33846/hn.v2i3.147

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Prevention of iron deficiency anemia in Indonesia using ferrous sulfas blood-added tablets started since 1997. But iron deficiency anemia is still high, also in young women. Young women are vulnerable because of rapid growth and unhealthy their consumption patterns, which rarely consume fruits and vegetables that are a source of vitamin C. The purpose of this study was to analyze the addition of vitamin C in iron supplementation + folic acid to serum ferritin levels in adolescents females anemia. This research was an experimental quasi research. Design of research was Randomized Pre Post Test Control Group Design, with Double Blind treatment. This study had one treatment group and one control group, each group of 11 samples. The samples were chosen randomly with inclusion and exclusion. The collected data was processed using SPSS program with the analysis of paired t-test and independent t-test to test the difference of serum ferritin level. The results was showed the characteristics of respondents: age (p = 0.430), education of mother (p = 0.942) and nutritional knowledge of respondents (p = 0.928) in the same condition or both groups were had homogeneous characteristics. The pattern and level of food consumption were showed no different between control and traetment groups (p> 0.05). Serum ferritin levels showed difference between the two groups after treatment (p = 0.004). The study was showed vitamin C had role in increasing iron absorption and fill in storage of ferritin formation. Keywords: Anemia, Vitamin C, Iron supplement, Ferritin
Green Tea (EGCG) Decreases Malondialdehide in Oxidative Stress Exposure to Psychological Stress M. Dodi F Priaminda; Bambang Wirjatmadi; Bambang Purwanto; Merryana Adriani
Health Notions Vol 2, No 7 (2018): July
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn20707

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Exposure to psychological stress can cause oxidative stress. Provision of exogenous antioxidants is expected to reduce the occurrence of oxidative stress. Green tea with its active ingredient Epigalocatechin Galat is considered to reduce the occurrence of oxidative stress. The purpose of this study was to examine the administration of green tea (EGCG) on the effect decreasing MDA levels against Wistar Strain (Rattus norvegicus) white rats exposed to psychological stress; with Randomized Post Test design of only group design by grouping into 5 groups, with control group and treatment group. Administration of steeping green tea (EGCG) with a single dose. To find out the significant influence of the independent variable on a set of variable group tested Manova. Post hoc testing using LSD (Least Significance Different) was performed to see significant differences between groups. In this study, obtained MDA data with significant decrease at the 6th hours after consum EGCG. Keywords: oxidative stress; green tea, EGCG, malondialdehide
Robusta Coffee Decreased Malondialdehyde Levels in Wistar Mice Experiencing Oxidative Stress Rahmi Syarifatun Abidah; Bambang Wirjatmadi; Bambang Purwanto; Merryana Adriani
Health Notions Vol 1, No 4 (2017): October-December
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (607.78 KB) | DOI: 10.33846/hn.v1i4.68

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Coffee drinking is a habit that still doubtful for it’s benefits. Many active ingredients in robusta coffee (Coffea canephora) are very useful for health, especially the antioxidant content that can ward off free radicals that absorbed in the body. Free radicals can be caused by psychological stress exposure. Excessive amounts of radicals in the body cause oxidative stress. This study aimed to see the robusta coffee effect on decreasing levels of Malondialdehyde (MDA) with “randomized post test only control group” design with 25 samples of white male rat strain wistar (Rattus norvegicus). Samples were divided into 5 groups: control groups and randomized treatment groups. Treatment given in psychological stress and giving robusta coffee drink with single dose. This study used “one way anova” test and followed by “pos hoc tukey HSD” test for statistics. Robusta coffee had an effect in MDA levels changing. There was a MDA levels decreasing in 1 hour after robusta coffee drinking in experimental rats with oxidative stress conditions that were exposed by psychological stress. Keywords: Robusta coffee, Malondialdehyde, Oxidative stress
Young khalaal dates (Phoenix dactylifera) effect on blood glucose in alloxan-induced wistar male rats Desty Muzarofatus Sholikhah; Bambang Wirjatmadi; Merryana Adriani
Health Notions Vol 2, No 1 (2018): January
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (467.658 KB) | DOI: 10.33846/hn.v2i1.102

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This study analyzed young dates (Phoenix dactylifera) effect on blood glucose in alloxan-induced male wistar rats. It used a completely randomized design with pre-post test and control group design. The research samples were 25 male white wistar rats that divided into 5 groups: (1) negative control group with no treatment (K1); positive control group with alloxan induction only (K2); treatment group 1 with alloxan induction and 0.25 g/day of young dates treatment (P1); treatment group 2 with alloxan induction and 0.5 g/day of young dates treatment (P2); and treatment group 3 with alloxan induction and 1 g/day of young dates treatment (P3). The result were tested by Kolmogorov-Smirnov normality test (p>0.05), Levene's homogeneity test (p>0.05), followed by General Multivariate Manova test by Wilks' Lambda, between-subject effect test, and LSD (p<0.05). The results indicated that young dates could effectively reduced the blood glucose levels (p = 0.000). The fasting blood glucose level in positive control group (K2) was different from negative control group (K1, p = 0.000) and all treatment groups (P1, p = 0.000, P2, p = 0.000 and P3, p = 0.000). 0.25 g/day of young dates treatment could effectively reduced the fasting blood glucose level in alloxan-induced male wistar rats. Keywords: young dates, blood glucose levels, alloxan
Cocoa liquor increases SOD activity in wistar rats experiencing oxidative stress Kartika Wahyu Dwi Putra; Bambang Wirjatmadi; Bambang Purwanto; Merryana Adriani
Health Notions Vol 2, No 1 (2018): January
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.472 KB) | DOI: 10.33846/hn.v2i1.98

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Chocolate is a healthy food. The active ingredient in chocolate (Theobroma cacao L.), especially antioxidants, can counteract the free radicals present in the body. This study aimed to determine the effect of cocoa liquor on increased activity of Superoxyde Dismutase (SOD). The study design was randomized post test only control group with 20 samples of male white rats wistar (Rattus norvegicus). The sample was divided into 4 groups: 2 control groups and 2 treatment groups. The treatments provided were instantaneous physical activity and cocoa liquor preparation with a single dose. This study used one way anova and continued with post hoc tukey HSD test for statistic. The results showed that cocoa liquor had an effect on SOD activity. SOD activity increased in treatment group with cocoa liquor either 2 hours or 24 hours before the momentary physical activity. Keywords: Cocoa liquor, Superoxide dismutase, Oxidative stress
Co-Authors Ade Saputra Nasution Adriani, Meriyana Adriani, Merriyana Ahmad Nur Huda AMBARWATI, SEPTIANA Amelia Dyah Kartika Sari Anak Agung Gede Sugianthara Andriani, Merryana Anindya Mar’atus Sholikhah Anisah Khoirul Umah Annisa Rizka Oktavianita Aprilia Nurcahyaning Rahayu Arinda Lironika Suryana Arwati, Niluh Astuti, Nanik Dwi Aulia, Kharina Auliannisaa, Alifia Ayuningtyas, Septiadeti Hidayati Bambang Purwanto Bambang Purwanto Bambang Purwanto Cindy Claudia Nurulita Civana Dea Alnanda Cut Athira Sauma D.A. Liona Dewi Damayanti, Adelina Elsa Desy Prasetya, Desy Diana Rizqi Fauziyah Dini Setiarsih Dwi Putra, Kartika Wahyu Dwi Winarni Eganda Garwahusada Emi Nur Cholidah Erliza Anggraini Firdaus Etha Rambung Fara Disa Durry Fatifa Asmarani, Fatifa Hadiar Huriyah Rahma Haqqelni Nur Rosyidah Harisma Harisma Himaya Himaya Ikanov Safitri INDROPO AGUSNI Isaura, Emyr Reisha Jayanthi Petronela Janggu Jujuk Proboningsih, Jujuk Kadek Tresna Adhi Kartika Indaswari Dewi Kartika Wahyu Dwi Putra Kharina Aulia Khoiroh, Ummul Kirana, Dhea Sukma Kuntoro Kuntoro Kuntoro Kuntoro Kurniasari, Nadya Putri Kusuma, Anindita M. Dodi F Priaminda M., Soenarnatalina Mangestuti Agil Maria Widijanti Sugeng Meirna Dewita Sari Merryana Adriani Merryana Adriyani Merryana Andriani Merryna Adriani Minarni Wartiningsih Mohammad Z. Rahfiludin Nabilla Siti Hawa Fatimah Nanik Dwi Astuti Novera Herdiani, Novera Novita Eka Hardini Nur Nashriana Jufri Nuraini, Sekarsari Oktovina Rizky Oktovina Rizky, Oktovina Pratama, Dafa Azhari Priaminda, M Dodi F Pudji Astutik, Pudji Raharja, Kristian Triatmaja Rahayu, Aprilia Nurcahyaning Rahmi Syarifatun Abidah Rahmi Syarifatun Abidah, Rahmi Syarifatun Retno Larasati Retno Larasati Rifka Laily Mafaza Rina Wasesa Aprilia Lazulfa Rinda Istiqumilaily Rivan Virlando Suryadinata Sari, Yulianti Wulan Setiarsih, Dini Setyaningtyas, Stefani Widya Sholikhah, Anindya Maratus` Sholikhah, Desty Muzarofatus Soenarnatalina M. Soenarnatalina Meilanani Sri Hartiningsih Sri Hartiningsih Sri Hartiningsih Sri Sumarmi Stefania W Setyaningtyas Supriyadi Supriyadi Suryadinata, Rivan Virlando Susanti, Yuyun Erlina Vidya Noermalawati Vyatri Pricilya Wardani, Karmila Ayu Yoes P. Dahlan Yunita Satya Pratiwi Yuyun Erlina Susanti Zahirah, Fildza Zolla Gracia Amardiani