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All Journal Jurnal Ilmu Pertanian Indonesia PHARMACON Jurnal Pendidikan Biologi Undiksha Wahana-Bio: Jurnal Biologi dan Pembelajarannya Jurnal Analis Kesehatan Media Farmasi Jurnal Kreativitas PKM Jurnal Ilmu Kedokteran dan Kesehatan Jurnal Medika Malahayati JFM (Jurnal Farmasi Malahayati) Jurnal Pengabdian Farmasi Malahayati (JPFM) Jurnal Analis Farmasi Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) JIIP (Jurnal Ilmiah Ilmu Pendidikan) Majalah Biomorfologi (Biomorphology Journal) Jurnal Abdimas Berdaya : Jurnal Pembelajaran, Pemberdayaan dan Pengabdian Masyarakat JAF (Jurnal Analis Farmasi) Innovative: Journal Of Social Science Research TeknoKreatif: Jurnal Pengabdian kepada Masyarakat JOURNAL OF Pharmacy and Tropical Issues
Dewi Chusniasih
Program Studi Biologi, Jurusan Sains, Institut Teknologi Sumatera
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Transportation Veterinary Other

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Journal : Jurnal Ilmu Pertanian Indonesia

 1 
Bioprospeksi Bakteri Asal Akar Nanas (Ananas comosus L. Merr) Lahan Gambut Kayu Agung, Sumatra Selatan, sebagai Agen Biostimulan dan Bioprotektan Erma Suryanti; Dewi Chusniasih; Muhammad Asril; Ika Agus Rini; Wulandari Putri Antika; Nadia Rahmah
Jurnal Ilmu Pertanian Indonesia Vol. 28 No. 3 (2023): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.28.3.352

Abstract

Pineapple (Ananas comosus L. Merr) is a plant that can live well on nutrient-poor lands such as peatlands. However, plant resistance to nutrient-poor conditions is caused by symbiosis with endophytic bacteria capable of plant growth-promoting bacteria (PGPB) through biostimulant and bioprotective mechanisms. This study aims to characterize the potential of endophytic bacteria from pineapple roots from peatlands as biostimulants and bioprotective agents. Thirteen endophytic bacteria from pineapple root were characterized by their ability as biostimulants through phosphate dissolution tests using Pikovskaya media qualitatively and quantitatively, and the production of indole-3-acetic acid (IAA) upon additional 0.1% tryptophan. Furthermore, bacteria as bioprotectors were characterized by qualitative production of chitinase enzyme and antifungal tests against Fusarium proliferatum using dual culture techniques. The results showed the presence of 2 positive bacteria in all tests, namely the ANAP3 and ANAP5 isolates. ANPA3 bacteria show the highest activity in IAA production (26.3 ppm), and the highest antifungal activity in inhibiting F. proliferatum, with an inhibitory index reaching 52.6%. Meanwhile, ANAP5 is an endophytic bacterium with the highest phosphate dissolving activity, with a dissolved phosphate value of 253.5 ppm. This finding shows that some endophytic bacteria from pineapple roots on peatlands can be biostimulants and bioprotectors that can be developed as PGPB. Keywords: Ananas comosus L. Merr, endophytic bacteria, bioprotectant, biostimulant, Fusarium proliferatum
Isolasi dan Uji Aktivitas Selulolitik Bakteri Asal Limbah Bagas Dewi Chusniasih; Erma Suryanti; Erina Safitri
Jurnal Ilmu Pertanian Indonesia Vol. 28 No. 3 (2023): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.28.3.386

Abstract

Bagasse is one of the by-products of granulated sugar industries or processed beverages that become waste in the environment. The bagasse will be overgrown by cellulolytic bacteria that produce cellulase enzymes. This study aims to isolate, characterize, and measure bacterial cellulase activity qualitatively and quantitatively. The research phase included isolation and characterization of bacteria, qualitative cellulase activity test, preparing standard curves and bacterial growth curves, crude extract cellulase production, constructing standard glucose curves, quantitative enzyme activity tests, and data analysis. Six isolates with macroscopic characteristics varied in color, elevation, margins, and colony shape; three bacillus isolates, one short bacillus isolate, and two cocci isolates were obtained. All isolates showed positive test results of amylum, triple sugar iron agar, and catalase; five citrate-positive isolates, and three motile-positive isolates. From the cellulase qualitative test, two bacterial isolates had the highest cellulolytic index, namely AT1 (1.79) and BAW3 (1.72) on 0.5% CMC media. The quantitative cellulase test results of the two selected isolates were 0.01176 U/mL for AT1 and 0.01170 U/mL for BAW3, both of which are still classified as low degradation capabilities. Keywords: cellulase activity, cellulolytic index, Sugarcane waste
Co-Authors Ade Maria Ulfa Agung Kurniawan Alfauzia, Nadia Anbar Istiadi, Khaerunissa ANDY DARMAWAN Anisa Putri Aprilia Andansari Arief Hidayat Arini Fauziah Susanti Aristina, Renny Ariza, Angga Dwi Arliani Nur Anisa, Hida Arum Dinda Bestari Azmi, Sarah Bian Tiara Putri Chusairil Pasa Dhea Virgiyanda Putri Dwi Susanti Eka Ananda Putri Erina Safitri Erma Suryanti Fitri Amelia Purmadi Fitri Rizki Oktariyani Gita Pratiwi Gres Maretta, Gres Gusti Ayu Rai Saputri Handayani, Catie Hellen Amelysa Hidayat, Thaariq Iffa Afiqa Khairani Ika Agus Rini Ismalia Husna Jannah, Umi Nur Jeane Siswitasari Mulyana Khaerunissa Anbar Istiadi Kharisma Aulia Putri Kurnia Wahyuni Lia Ermawati Marcellia, Selvi Maria Ulfa, Ade Michelle Angeline Muhammad Asril Muhammad Iqbal Farhan Nadia Rahmah Nadia Syakira Najla Nur Azizah Natalia Pardede, Desta Ni Galuh Putri Nandini Ni Komang Astriani Nofita Nofita Novisa Arizatul Fikriana Novrianti, Rima Arini Putri, Ria Desta Rediyanto Rizki Yuliana Rahayu Salsa Pratiwi Mulyadi Santi, Putri Era Sari, Dian Anggria Sari, Rara Mulia Selvi Marcellia Selvi Marcellia Selvi Marcellia Sinaga, Ayu Oshin Yap Siti Nurul Alfiah Sobirin Sobirin Solly Aryza Stevan Ronald Gulo Sukardi Sukardi Suryanti, Erma Theodora Octavia Tiara Jeni Anjelina Tutik Tutik Tutik Tutik Tutik Tutik Ulfa , Ade Maria Vida Elsyana Vida Elsyana Wahyuni Dian Lestari Winati Nurhayu Wulandari Putri Antika Yunita Fahni