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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Fitopatologi Indonesia Zuriat Jurnal AGROTEKNOLOGI Jurnal Ilmiah Aplikasi Isotop Radiasi JURNAL ILMU PERTANIAN Agrifor : Jurnal Ilmu Pertanian dan Kehutanan Jurnal Biologi Universitas Andalas Jurnal Agroindustri AGRITEPA: Jurnal Ilmu dan Teknologi Pertanian JURNAL AGRILAND BIOMA : Jurnal Biologi Makassar JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Agrosains: Jurnal Penelitian Agronomi Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Journal of Applied Agricultural Science and Technology JURNAL AGROHITA Lumbung: Jurnal Ilmiah Politeknik Pertanian Negeri Payakumbuh Buletin Ilmiah Nagari Membangun Jerami Unri Conference Series: Agriculture and Food Security Jurnal Teknologi Pertanian Andalas Jurnal Agronomi Tanaman Tropika (JUATIKA) Agroteknika Agrotechnology Research Journal Jurnal Pendidikan Tata Boga dan Teknologi Jurnal Triton Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Bioscientist : Jurnal Ilmiah Biologi Fruitset Sains : Jurnal Pertanian Agroteknologi Jurnal Penyuuhan dan Pemberdayaan Masyarakat (JPPM) Jurnal Riset Perkebunan (JRP) Journal of Applied Agricultural Science and Technology Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi Jagur: Jurnal Agroteknologi
Yusniwati Yusniwati
Andalas University
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Transportation Veterinary Other

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Journal : Journal of Applied Agricultural Science and Technology

 1 
Effect of Ultraviolet-C Irradiation on Morphological Character Changes in Patchouli (Pogostemon cablin Benth.) Haryanti, Sindi; Yusniwati, Yusniwati; Gustian, Gustian
Journal of Applied Agricultural Science and Technology Vol. 8 No. 3 (2024): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v8i3.284

Abstract

Patchouli (Pogostemon cablin Benth.) is among the top 20 essential oil-producing plants traded in the global market. This plant shows narrow genetic diversity due to non-flowering and seed production, increasing the challenges in obtaining new varieties through crossbreeding. Therefore, this research aimed to apply mutation method using ultraviolet-C irradiation combined with tissue culture to broaden the genetic diversity of patchouli. The potential of UV-C irradiation and the morphological changes occurring in the plant were explored from October to December 2023 at the Tissue Culture Laboratory, Andalas University, Padang. In addition, the experiment was arranged in a Randomized Complete Block Design (RCBD) with 10 treatment levels and 3 groups. The treatments included UV-C irradiation doses of 0 (wild type), 30, 60, 90, 120, 150, 180, 210, 240, and 270 minutes at a distance of 30 cm. Data for each patchouli plant per observation variable were presented as mean values, variance, and standard deviation and analyzed using an unpaired t-test. The results showed that exposure to ultraviolet-C radiation impacted several morphological characteristics, such as chlorosis, delayed bud development, increased bud and leaf count, as well as faster bud growth. This phenomenon shows the potential of ultraviolet-C radiation as a physical mutagenic agent.
Effect of the Combination of the Growth Regulators and Putrescine on the Somatic Embryogenesis of Wheat (Triticum Aestivum L.) on Some Types of Explants Yusniwati, Yusniwati; Setiawan, Ryan Budi
Journal of Applied Agricultural Science and Technology Vol. 7 No. 4 (2023): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v7i4.158

Abstract

Plant breeding programs need to be carried out in order to improve the genetics of wheat that is able to adapt to tropical environments through hybridization, mutation induction, tissue culture, and genetic transformation.  In vitro culture through somatic embryogenesis pathways plays an important role in genetic improvement and its integration with other breeding programs can positively affect the improvement of wheat quality, quantity, and development in Indonesia. The purpose of this study was to obtain an embryogenic callus induction method from the Dewata variety using five different types of explants, namely mature Seeds, immature embryos, immature seeds, leaf,  stem, and to obtain combination of plant growth regulators and putrescine on somatic embryogenesis of wheat.   The experimental design was prepared based on a complete randomized design with a combination treatment of embryogenic callus induction media consisting of 9 levels, namely: 1 ppm 2.4- D, 1 ppm 2.4-D + 1 ppm Picloram, ppm 2.4-D + 1 mg / L Picloram, 1 ppm 2.4-D + 10-4  M Putrescine, 1 ppm 2.4-D + 1 ppm Picloram +10-4  M Putrescine, 2 ppm 2.4-D + 1 ppm Picloram + 10-4  M Putrescine, 1 ppm 2.4 D + 10-3 M Putrescine, 1 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine, 2 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine. The results showed that the media used was able to induce embryogenic callus using mature seed and immature embryo, but  immature seed and leaf were not able to produce embryogenic callus. The best media  that produced the highest percentage of embryogenic callus was 2 ppm 2.4-D + 1 ppm Picloram + 10-4  M Putrescine with as much as 85.9% in young embryo explants.
Co-Authors . Reflinaldon Afrizal Nofri Agus Sutanto Agus Sutanto Alfi Asben Anwar Kasim Anwar, Aswaldi Apriany Fatmawaty, Andi Aprissilia Taifani Galuh Utomo Aprizal Zainal Arief Laksana Bosma, Pandu Aries Kusumawati Auzar Syarif Azka , Yuliantina Baiturrahman, Atzim Bambang Hariyanto Benni Satria Bosma, Pandu Arif Laksana Budi, Rahmad Setia Daimon Syukri Damayanti, Elok Deivy Andhika Permata Dendy Tri Dharma Putra Denny Yulfa Dewi Rezki Dharmawan , Dhani Nur Dian Sukma, Dian Etti Swasti Fadilla Arishadea Febriani Febriani Gusti Marlina Gustian Gustian Gustian Hafnes Wahyuni Haliatur Rahma, Haliatur Handayani, Mellyyana Haryanti, Sindi Hasbullah Hasbullah Hayati, PK Dewi Hidrayani Hidrayani Illahi, Ayu Kurnia Indri Yanil Vajri Irfan Suliansyah Ismed Ismed Jumsu Trisno Khairunnisa, Nadia KRISTINA, NILLA Lily Syukriani Magdalena Saragih Magdalena Saragih Saragih Mismawarni Srima Ningsih Mustikarani, Eries Dyah Nalwida Rozen Nanda, Alifma Rahimmi Nasution, Fauzan Abid Hanif Neswati Neswati Netti Herawati Nissa, Ayu Khairun Nofialdi, Nofialdi Noflindawati Noflindawati Nofrita Sandi Nova Reskhi Firdaus Novizar Nazir Nurbailis Nurmayulis Obel, Obel Prana Dipa Tiarani Prana Dipa Tiarani Purnama Sari, Indah Putri Salsa Afifah Rahma Wati, Dela Rahmi Holinesti Rahmi, Alfazlul Reni Mayerni Rina Yenrina Roza Yunita Ryan Budi Setiawan Sahroni Ridoi Saragih, Magdalena Sembiring, Rinawati Siska Efendi Sobrizal Sobrizal Sri Wahyuni Susiyanti, Susiyanti Syahbanuari Sitompul Syahputra, Agil Syukri Arif Trizelia . Tuty Anggraini Utomo, Aprissilia Taifani Galuh Winarto Winarto Yaherwandi Zahlul Ikhsan Zainal , Aprizal Zurai Resti