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Zulfa Zakiah
Universitas Tanjungpura
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Energy Environmental Science Immunology & microbiology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Other

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In Vitro Shoot Culture Growth of Pontianak Orange (Citrus nobilis var. microcarpa) with the Addition of Naphthalene Acetic Acid and 6-Benzyl Amino Purine Nissa, Zoelva Zakiatun; Zakiah, Zulfa; Turnip, Masnur
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6344

Abstract

Pontianak siam orange (Citrus nobilis var. microcarpa) is an important agricultural commodity unique to Sambas Regency, West Kalimantan. The demand for Pontianak siam oranges has been steadily increasing over the years, yet the production of Pontianak oranges has not been able to meet the domestic demand. The limited availability of superior seedlings is one of the reasons for this situation. Therefore, efforts are needed to fulfill the demand for Pontianak siam oranges by producing a large quantity of superior seedlings in a short period. One of the strategies that can be employed is in vitro propagation. This research aims to observe the growth of Pontianak Siamese orange shoot explants after administration of NAA (Naphthalene Acetic Acid) and BAP (6-Benzyl Amino Purine). This research was conducted from January to June 2023 at the Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University. The study employed a Completely Randomized Design (CRD) with two treatment factors namely NAA concentrations with 4 levels (0; 0.25; 0.5; 0.75 mg/L), and BAP concentrations with 5 levels (0; 0.5; 1; 1.5; 2 mg/L). Parameters observed were the emergence time of shoot, shoot number, height of shoot, and the number of leaves. The results showed that single NAA, single BAP, and combination treatment of NAA and BAP significantly affected all growth parameters. Treatment with the addition of 0.25 mg/L NAA and 1 mg/L BAP (A1B2) was the treatment with the fastest shoot emergence time (2.80 days after planting), the largest number of shoots (2.60 shoots)the tallest shoots (1.50 cm), , and the greatest number of leaves (2.60 leaves).
Induksi Kalus dari Hipokotil Belimbing Merah (Baccaurea angulata) dengan Penambahan 2,4-D (Dichlorophenoxy Acetic Acid) dan BAP (6-Benzyl Amino Purin) Teresia, Novi; Zakiah, Zulfa; Turnip, Masnur
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6387

Abstract

Red starfruit is one of the endemic plants of Kalimantan which produces secondary metabolites which has been empirically used as antioxidant, antibacterial, and anticancer. This plant has a long reproductive period and seedless fruit is often found during the fruiting season, which is a problem for the sustainability of the red starfruit in nature. Conservation of red star fruit for propagation and production of secondary metabolites can be done in vitro through callus culture. This research aims to determine the effect of adding the growth regulators dichlorophenoxy acetic acid (2,4-D) and 6-benzyl amino purine (BAP) on callus induction time, percentage of callus forming explants, callus color and texture, and callus growth. This research used a Completely Randomized Design (CRD) with a factorial pattern consisting of two treatment factors, namely 2,4-D (0; 0.25; 0.5; 0.75; 1 ppm) and BAP (0; 0.25; 0.5; 0.75; 1 ppm). The percentage of explants forming callus (%), the time the callus appeared (days after planting), the color and texture of the callus, the wet weight and dry weight of the callus (g) were research observation parameters.The results showed that the single factor 2,4-D, single factor BAP, and the interaction of 2,4-D and BAP had a significant effect to callus appearance time. Single administration of 2,4-D and BAP had a significant effect on callus dry weight. The color variations of the callus produced are white, green and brown with a compact texture. The fastest callus emergence time was at 11.00 days after planting (DAP), namely in the combination treatment of 1 ppm 2,4-D and 0.75 ppm BAP, single treatment 0.75 ppm and 1 ppm 2,4- D. The highest average dry weight of 0.024 g was obtained in the single treatment of 0.75 ppm BAP.
Induction of Somatic Embryogenesis of Pontianak Siamese Orange Cotyledon Cultures on Murashige Skoog Media with the Addition of 2,4-D and Kinetin Zakiah, Zulfa; Turnip, Masnur
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6721

Abstract

Conventional citrus propagation has weaknesses, one of which is susceptible to CVPD(Citrus Vein Phloem Degeneration) and other diseases which are the main cause of the decline in population and production of pontianak siam orange plants in West Kalimantan. One alternative to in vitro propagation of citrus seedlings is the induction of somatic embryogenesis. The purpose of the study was to obtain the best combination of 2,4-D and kinetin concentrations for callus induction and somatic embryo induction from Pontianak siam orange cotyledon explants, observing the developmental phase (stage) of embryos formed. The implementation of the study used a factorial completely randomized design (CRD) consisting of 2 factors. The first factor is the concentration of 2,4-D consisting of 4 concentration levels, namely 0; 0.5; 1; 1.5; 2 mg/L. The second factor is kinetin concentration consisting of 4 concentration levels, namely 0; 0.5; 1; 1.5; 2 mg/L.The observation parameters include the time of callus emergence (hst), the percentage of explants forming callus (%), the texture and color of the callus, observing the stage of the embryo found. The results showed that 72% of treatments were able to induce callus from pontianak siam orange cotyledons with an average callus appearance at 7 hst. The callus formed is compact and crumbly with varied callus colors, namely white, yellowish, greenish and brownish. The best treatment that produces embryogenic callus is the combination of D0.5K0.5; D1K1; D0.5K1.5; D1.5K1.5 and D2K2 with the characteristics of crumbly callus and yellowish and brownish colors. The results of microscopic observations found that the somatic embryo phase formed was the pro-embryo phase.
Potential of Mango Leaf Methanol Extract (Mangifera indica L.) as A Bioherbicide Against The Growth of Putri Malu Weed (Mimosa pudica L.) Adellya Nabilla Putrie; Zakiah, Zulfa; Mukarlina, Mukarlina
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6747

Abstract

Putri Malu (Mimosa pudica L.) is a weed that often found in various places, especially on agricultural land and plantations. Mangifera indica L. has potential as a bioherbicide because it contains aleochemical compounds, which inhibit the growth of other plants. This research aims to determine the potential and the best concentration of methanol extract of mango leaves (Mangifera indica L.) in inhibiting the growth of putrimalu weed (Mimosa pudica L.). This research was carried out for four months, in the Kasa House Laboratory, Biology Department, Faculty of Mathematics and Natural Sciences, Tanjungpura University. The research used a Completely Randomized Design (CRD) with five mango leaf extract concentration treatment levels, namely control (P0), 10% (P1), 20% (P2), 30% (P3), and 40% (P4). Each treatment was repeated five times to obtain 25 experimental units. The results showed that the methanol extract of mango leaves (Mangifera indica L.) had an effect on inhibiting the germination and growth of the M. pudica weed. The best concentration of methanol extract of mango leaves in inhibiting the germination and growth of putrimalu weed is a concentration of 20%.
The Growth of Epicotyl Shoots of Glutinous Corn (Zea mays L. var. ceratina) on Murashige Skoog Medium with NAA and Coconut Water Delviera, Dhea Amanda; Zakiah, Zulfa; Turnip, Masnur
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.8081

Abstract

Glutinous corn (Zea mays L. var. ceratina) is a local variety known for its distinctive traits, particularly its high amylopectin content, comprising 72% amylopectin and 28% amylose. This research aims to explore the impact of incorporating Naphthalene Acetic Acid (N.A.A.) and coconut water and identify the optimal concentrations that influence the growth of epicotyl shoots in glutinous corn. The study was conductedk in the Tissue Culture Laboratory at the Department of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University in Pontianak. A completely randomized design (C.R.D) with factorial arrangements was employed. The first factor considered various concentrations of NAA (0, 0.5, 1.0, and 1.5 ppm), while the second factor involved coconut water at different concentrations (0%, 15%, 30%, and 60%). The findings indicated that the combination of NAA and coconut water significantly impacted the shoot emergence time and height. The combinations of 0.5 ppm NAA with 15% and 30% coconut water resulted in the fastest shoot growth, averaging 2.4 days after seed treatment (hst). In contrast, using 30% coconut water alone, without NAA, produced the tallest shoots, averaging 3.48 cm. However, the growth of glutinous corn epicotyl shoots was optimal only until 14 days of age.
Co-Authors Ade Indriani Adellya Nabilla Putrie Afghani Jayuska Amalia Rahman Anggun Melani Anna Rizkia Antoni Antoni Arbayah H. Siregar Ari Hepi Yanti Bayu Dwi Prawiga Delviera, Dhea Amanda Desika Saputri Desy Herawati Herawati Diah Wulandari Rousdy Duwi Kurnia Nugraheni Dwi Gusmalawati Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Erly Marwani Fadhlullah, Hafizh Rizqi Firman Saputra Fitri, Anjelina Hani Hani Harahap, Zulfan Hendri Hendri Holyness Nurdin Singadimedja Iir Purwanto Indrasari, Dita Indriani, Ade Irwan Lovadi Jayuska, Afghani Junardi Junardi Kustiati Kustiati Laely Rizqi Larasati Ningsih Lena Dian Saputri Lianema Doy Meilani Luh Putu Ratna Sundari Masnur Turnip Muhammad Rezky Abrar Mukarlina Mukarlina -, Mukarlina Mukarlina, Mukarlina Murni Murni Niluh Kharina Asoka Putri Nissa, Zoelva Zakiatun Nova Nova Nurkapita, Nurkapita Pancaning Wardoyo pardosi, immanuel kristian Perdana, Aurendyo Yuscel Rafdinal Rafdinal Rahmawati rahmawati rahmawati rahmawati Rahmawati, Rahmawati Rikhsan Kurniatuhadi, Rikhsan Rina Karmila Riska Windiyanti Riyandi Riyandi Riza Linda Riza Linda Riza Linda Ruqiah Ganda Putri Panjaitan Ruth Isabella Elisabeth Sari Mutiara Sianturi Safitri Lusiana Saputra, Firman selno, Silvester Siti Ifadatin Siti Khotimah Siti Khotimah Surya Abi Alfariz Sylvia Windy Teresia, Novi Turnip, Masnur Widasari, Rizka Yoanli Theresa Dea Zulfan Harahap