Claim Missing Document
Check
Articles

Induksi Kalus dari Hipokotil Belimbing Merah (Baccaurea angulata) dengan Penambahan 2,4-D (Dichlorophenoxy Acetic Acid) dan BAP (6-Benzyl Amino Purin) Teresia, Novi; Zakiah, Zulfa; Turnip, Masnur
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6387

Abstract

Red starfruit is one of the endemic plants of Kalimantan which produces secondary metabolites which has been empirically used as antioxidant, antibacterial, and anticancer. This plant has a long reproductive period and seedless fruit is often found during the fruiting season, which is a problem for the sustainability of the red starfruit in nature. Conservation of red star fruit for propagation and production of secondary metabolites can be done in vitro through callus culture. This research aims to determine the effect of adding the growth regulators dichlorophenoxy acetic acid (2,4-D) and 6-benzyl amino purine (BAP) on callus induction time, percentage of callus forming explants, callus color and texture, and callus growth. This research used a Completely Randomized Design (CRD) with a factorial pattern consisting of two treatment factors, namely 2,4-D (0; 0.25; 0.5; 0.75; 1 ppm) and BAP (0; 0.25; 0.5; 0.75; 1 ppm). The percentage of explants forming callus (%), the time the callus appeared (days after planting), the color and texture of the callus, the wet weight and dry weight of the callus (g) were research observation parameters.The results showed that the single factor 2,4-D, single factor BAP, and the interaction of 2,4-D and BAP had a significant effect to callus appearance time. Single administration of 2,4-D and BAP had a significant effect on callus dry weight. The color variations of the callus produced are white, green and brown with a compact texture. The fastest callus emergence time was at 11.00 days after planting (DAP), namely in the combination treatment of 1 ppm 2,4-D and 0.75 ppm BAP, single treatment 0.75 ppm and 1 ppm 2,4- D. The highest average dry weight of 0.024 g was obtained in the single treatment of 0.75 ppm BAP.
Induction of Somatic Embryogenesis of Pontianak Siamese Orange Cotyledon Cultures on Murashige Skoog Media with the Addition of 2,4-D and Kinetin Zakiah, Zulfa; Turnip, Masnur
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6721

Abstract

Conventional citrus propagation has weaknesses, one of which is susceptible to CVPD(Citrus Vein Phloem Degeneration) and other diseases which are the main cause of the decline in population and production of pontianak siam orange plants in West Kalimantan. One alternative to in vitro propagation of citrus seedlings is the induction of somatic embryogenesis. The purpose of the study was to obtain the best combination of 2,4-D and kinetin concentrations for callus induction and somatic embryo induction from Pontianak siam orange cotyledon explants, observing the developmental phase (stage) of embryos formed. The implementation of the study used a factorial completely randomized design (CRD) consisting of 2 factors. The first factor is the concentration of 2,4-D consisting of 4 concentration levels, namely 0; 0.5; 1; 1.5; 2 mg/L. The second factor is kinetin concentration consisting of 4 concentration levels, namely 0; 0.5; 1; 1.5; 2 mg/L.The observation parameters include the time of callus emergence (hst), the percentage of explants forming callus (%), the texture and color of the callus, observing the stage of the embryo found. The results showed that 72% of treatments were able to induce callus from pontianak siam orange cotyledons with an average callus appearance at 7 hst. The callus formed is compact and crumbly with varied callus colors, namely white, yellowish, greenish and brownish. The best treatment that produces embryogenic callus is the combination of D0.5K0.5; D1K1; D0.5K1.5; D1.5K1.5 and D2K2 with the characteristics of crumbly callus and yellowish and brownish colors. The results of microscopic observations found that the somatic embryo phase formed was the pro-embryo phase.
Potential of Mango Leaf Methanol Extract (Mangifera indica L.) as A Bioherbicide Against The Growth of Putri Malu Weed (Mimosa pudica L.) Adellya Nabilla Putrie; Zakiah, Zulfa; Mukarlina, Mukarlina
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6747

Abstract

Putri Malu (Mimosa pudica L.) is a weed that often found in various places, especially on agricultural land and plantations. Mangifera indica L. has potential as a bioherbicide because it contains aleochemical compounds, which inhibit the growth of other plants. This research aims to determine the potential and the best concentration of methanol extract of mango leaves (Mangifera indica L.) in inhibiting the growth of putrimalu weed (Mimosa pudica L.). This research was carried out for four months, in the Kasa House Laboratory, Biology Department, Faculty of Mathematics and Natural Sciences, Tanjungpura University. The research used a Completely Randomized Design (CRD) with five mango leaf extract concentration treatment levels, namely control (P0), 10% (P1), 20% (P2), 30% (P3), and 40% (P4). Each treatment was repeated five times to obtain 25 experimental units. The results showed that the methanol extract of mango leaves (Mangifera indica L.) had an effect on inhibiting the germination and growth of the M. pudica weed. The best concentration of methanol extract of mango leaves in inhibiting the germination and growth of putrimalu weed is a concentration of 20%.
The Growth of Epicotyl Shoots of Glutinous Corn (Zea mays L. var. ceratina) on Murashige Skoog Medium with NAA and Coconut Water Delviera, Dhea Amanda; Zakiah, Zulfa; Turnip, Masnur
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.8081

Abstract

Glutinous corn (Zea mays L. var. ceratina) is a local variety known for its distinctive traits, particularly its high amylopectin content, comprising 72% amylopectin and 28% amylose. This research aims to explore the impact of incorporating Naphthalene Acetic Acid (N.A.A.) and coconut water and identify the optimal concentrations that influence the growth of epicotyl shoots in glutinous corn. The study was conductedk in the Tissue Culture Laboratory at the Department of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University in Pontianak. A completely randomized design (C.R.D) with factorial arrangements was employed. The first factor considered various concentrations of NAA (0, 0.5, 1.0, and 1.5 ppm), while the second factor involved coconut water at different concentrations (0%, 15%, 30%, and 60%). The findings indicated that the combination of NAA and coconut water significantly impacted the shoot emergence time and height. The combinations of 0.5 ppm NAA with 15% and 30% coconut water resulted in the fastest shoot growth, averaging 2.4 days after seed treatment (hst). In contrast, using 30% coconut water alone, without NAA, produced the tallest shoots, averaging 3.48 cm. However, the growth of glutinous corn epicotyl shoots was optimal only until 14 days of age.
Karakteristik dan Kualitas Nata De Cane dari Nira Tebu (Saccharum officinarum L.) dengan Penambahan Ekstrak Kedelai (Glycine max L.) sebagai Sumber Nitrogen Wigiyanti, Vitriya; Zakiah, Zulfa; Rahmawati, Rahmawati
Bioma : Berkala Ilmiah Biologi Vol. 25 No 2, Tahun 2023
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2023.58419

Abstract

Sugarcane sap is a liquid found in sugarcane stalks, which can be drunk directly as a refreshment and as a basic ingredient for making granulated sugar. The nutritional content contained in sugarcane juice can be a growth medium for Acetobacter xylinum bacteria forming cellulose (nata). One of the factors supporting the growth of Acetobacter xylinum bacteria is a nitrogen source. This study aims to determine the effect and the best concentration of soybean extract on the quality characteristics of nata de cane. The research design used was a completely randomized design (CRD) with 7 concentrations of nitrogen sources including control (0%), 1% ZA, 2.5%, 5%, 7.5%, 10% and 12.5% soybean extract. Each treatment consisted of 4 replications. Parameters observed were the thickness and fiber content of nata and then analyzed using ANOVA (Analysis of Variance). Based on the results of the study, the addition of soybean extract (Glycine max L.) had an effect on the thickness and fiber content of nata de cane compared to the control treatment. The resulting Nata has a chewy texture and a distinctive aroma of fragrant sugarcane juice. The highest thickness and fiber content of nata de cane was obtained in the 12.5% soybean extract treatment, namely 1.58 cm and 2.95% and has met the quality requirements of SNI 01-4317-1996. Overall, the most preferred nata de cane organoleptic test result was nata de cane with the addition of soybean extract at a concentration of 12.5% in the very like category. The reseach results can be recommended to the public that soybean extract has the potential as an alternative to ZA in the process of making nata.
Potensi Ekstrak Buah Jambu Tangkalak (Bellucia pentamera Naudin) sebagai Penghambat Pertumbuhan Escherichia coli ATCC 25922 dan Staphylococcus aureus Windiyanti, Riska; Khotimah, Siti; Zakiah, Zulfa
Life Science Vol 12 No 1 (2023): May 2023
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v12i1.66777

Abstract

Escherichia coli dan Staphylococcus aureus merupakan bakteri patogen yang dapat menyebabkan penyakit gangguan saluran pencernaan dan infeksi. Salah satu tumbuhan obat yang dapat digunakan sebagai antibakteri adalah buah jambu tangkalak (Bellucia pentamera). Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak buah jambu tangkalak dan konsentrasi yang dapat menghambat pertumbuhan E. coli dan S. aureus serta golongan senyawa metabolit sekunder. Pengujian aktivitas antibakteri dilakukan menggunakan metode difusi cakram. Uji aktivitas antibakteri dilakukan dengan beberapa perlakuan konsentrasi yaitu 200; 400; 600; dan 800 mg/mL serta kontrol positif antibiotik kloramfenikol 0,03 mg/mL. Hasil uji fitokimia menunjukkan bahwa ekstrak buah B. pentamera mengandung golongan senyawa alkaloid, flavonoid, terpenoid, saponin, dan tanin yang berpotensi sebagai antibakteri. Ekstrak buah jambu tangkalak berpengaruh terhadap pertumbuhan bakteri E. coli dan S. aureus. Konsentrasi 200 mg/mL ekstrak buah jambu tangkalak sudah mampu menghambat dengan zona hambat 3,82 mm (24 jam) untuk bakteri E. coli sedangkan untuk bakteri S. aureus menghasilkan zona hambat 7,12 mm (24 jam) dan 8,37 mm (48 jam). Hasil uji aktivitas antibakteri menunjukkan bahwa semua perlakuan konsentrasi ekstrak mampu menghambat pertumbuhan bakteri berpotensi sebagai bakteriostatik dengan kategori lemah. kata kunci: Antibakteri; Bellucia pentamera; Ekstrak; Escherichia coli; Staphylococcus aureus
Inventarisasi Gulma Berdaun Lebar di Kebun Jagung (Zea Mays L.) Desa Rasau Jaya Umum Kabupaten Kubu Raya Provinsi Kalimantan Barat Rezki, Diskia; Zulfa Zakiah; Ifadin, Siti
JURNAL BIOLOGICA SAMUDRA Vol 5 No 2 (2023): Biologica Samudra
Publisher : Program Studi Biologi, Fakultas Teknik, Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jbs.v5i2.4572

Abstract

Jagung (Zea mays L.) merupakan komoditas dan sumber karbohidrat utama kedua setelah beras yang banyak dibudidayakan di Kalimantan Barat. Salah satu penyebab rendahnya produksi jagung adalah gulma yang mengakibatkan penurunan produksi mencapai 51%. Penelitian ini bertujuan untuk mengetahui jenis gulma berdaun lebar yang ada pada lahan kebun jagung di desa Rasau Jaya Umum Kabupaten Kubu Raya. Penelitian telah dilakukan dari bulan April sampai Juni 2021. Penelitian ini menggunakan metode jelajah (Cruise Method). Hasil penelitian telah ditemukan 14 spesies gulma yang termasuk ke dalam 11 famili yaitu Cleome rutidosperma, Oldelandia corymbosa, Ludwigia octovalvis, Perilla frutescens, Euphorbia hirta, Asystasia gangetica, Croton hirtus, Phyllathus ninuri, Lindernia crustacean, Ageratum conyzoides, Richardia scabra, Melastoma malabathricum, Boerhavia erecta, dan Physalis angulata.
Pelatihan Pengolahan Air Kelapa menjadi Nata de Coco di Desa Sungai Kakap, Kalimantan Barat Rahmawati, Rahmawati; Khotimah, Siti; Mukarlina, Mukarlina; Zakiah, Zulfa; Saputra, Firman; Turnip, Masnur; Lisandra, Aotza
Jurnal ABDINUS : Jurnal Pengabdian Nusantara Vol 10 No 1 (2026): Volume 10 Nomor 1 Tahun 2026
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/ja.v10i1.23985

Abstract

Old coconut water is often discarded by the community as waste. This community service activity aimed to provide training on processing coconut water into nata de coco for the members of the Family Welfare Empowerment Group (PKK) in Sungai Kakap Village, Kubu Raya Regency, West Kalimantan. The implementation of this community service program consisted of several stages, including dissemination/socialization, hands-on practice, and discussion sessions. The activity began with the preparation of tools and materials at the Biology Laboratory, Faculty of Mathematics and Natural Sciences, Tanjungpura University (FMIPA UNTAN), followed by a field visit to the target village, participant gathering, socialization of nata de coco production materials, joint practice with workshop participants, and a discussion session. The nata de coco production training was successfully carried out at the Sungai Kakap Village Office, West Kalimantan. Based on the questionnaire results, the PKK members stated that the activity was beneficial, and many expressed their interest in applying the process and producing nata de coco for personal consumption as well as for potential commercialization.