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PENGARUH BIOSTIMULAN EKSTRAK DAUN CENGKODOK (Melastoma malabathricum L.) TERHADAP PERTUMBUHAN DAN STRUKTUR ANATOMI PLANLET ANGGREK HITAM (Coelogyne pandurata Lindl) PADA TAHAP AKLIMATISASI Zakiah, Zulfa; Turnip, Masnur
BUANA SAINS Vol 24, No 1 (2024)
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/bs.v24i1.5815

Abstract

Cultured plants will adapt to new environmental conditions at the acclimatization stage. Changes that occur can observed through changes in morphological and anatomical structures.  The increase in the number of cells and the size of plant organs illustrates the process of changing the anatomical structure of plants. It is necessary to optimize the concentration of biostimulant given and see its effect on plant growth and anatomical structure, to explain the mechanism of action of biostimulants. The research aimed to determine the concentration of biostimulant cengkodok leaf extract on black orchid plants. The study used a completely randomized design consisting of 8 treatment levels of cengkodok leaf extract biostimulant concentration (0, 10, 20, 30, 40, 50, 60 and 70 mg/L).  The results showed that the concentration of cengkodok leaf extract 20 mg/L was the best concentration for the parameters of the number of leaves and the number of roots of black orchid planlets, respectively 2.5 leaves and 7.5 roots. The application of cengkodok leaf extract affects the anatomical structure characteristics of black orchids, including an increase in the thickness of leaf mesophyll tissue, stem cortex tissue, and roots.
Growth of Black Orchids (Coelogyne Pandurata Lindl) with Additional Ecoenzyme Liquid Organic Fertilizer and Biostimulant of Cengkodok Leaf Extract Zulfa Zakiah; Masnur Turnip
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.6113

Abstract

Acclimatization of black orchid plantlets (Coleogyne pandurata Lindl.) with cengkodok leaf extract biostimulant treatment has been successfully carried out, but still have slow growth. To support growth and development at the enlargement stage, cultivated orchids need to be given eco enzyme liquid organic fertilizer (LOF) derived from various sources of organic matter. Provision of ecoenzyme LOF combined with biostimulant 20 mg/l of Cengkodok leaf extract to increase the efficiency of nutrient absorption. The aim of the research was to find the best type and concentration of LOF for the growth of black orchids at the enlargement stage. The study used a Complete Randomized Design (CRD) with two treatment factors namely type of LOF (ecoenzyme (ee) LOF from vegetable and fruit waste, ee LOF from pineapple peel waste, and "NASA" LOF) and . the POC concentration (0; 0.5; 1.0; and 1.5 ml/l). The results showed that the single treatment of ee LOF significantly effected the number of leaves and the number of black orchid tillers, while the single treatment of ee LOF concentration had an effect on the parameters of height, number of leaves, number of tillers and leaf area of black orchid. The parameter of chlorophyll content had no signifcant effect by the type and concentration of LOF. The largest leaf area was shown in the addition of ee LOF from vegetable and fruit waste at a concentration of 1 ml/l which was 11.88 cm2.
Callus growth of red starfruit (Baccaurea angulata) leaves with the addition 2,4-D (dichlorophenoxy acetic acid) and kinetin Zulfa Zakiah; Murni Murni; Masnur Turnip
Symbiotic: Journal of Biological Education and Science Vol. 5 No. 1 (2024): Symbiotic: Journal of Biological Education and Science Vol. 5 No. 1 April 2024
Publisher : Institut Agama Islam Negeri Kerinci

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32939/symbiotic.v5i1.102

Abstract

One of the obstacles to propagating red starfruit (Baccaurea angulata) using seeds is that fruit without seeds is often found. Tissue culture is an alternative for propagating red star fruit plants to provide seeds or secondary metabolites through callus culture. The research aims to determine the effect of 2,4-D and kinetin on callus growth of star fruit leaves and to obtain the best concentration to induce callus. This research used a factorial Completely Randomized Design (CRD) with two treatment factors, namely 2,4-D (0; 0.5; 1.0; 1.5; 2.0; 2.5 ppm) and kinetin (0; 0.5; 1.0; 1.5; 2.0; 2.5 ppm). The observation parameters consisted of callus emergence time (days after planting), wet and dry weight of the callus (g), color and texture of the callus. The results showed that the combination of 2,4-D and kinetin had a significant effect to callus emergence time, but didn't on the wet and dry weight of the callus. The fastest callus emergence time was obtained of 2.5 ppm 2,4-D + 0.5 ppm kinetin, 1.5 ppm 2,4-D + 2.5 ppm kinetin namely 6 DAP. The callus color is white, brown, and brown with a friable and compact texture. Another response that emerged was root growth.
Pelatihan Pembuatan Virgin Coconut Oil (VCO) Bagi Ibu-Ibu PKK di Desa Sungai Kakap Kabupaten Kubu Raya Khotimah, Siti; Zakiah, Zulfa; Mukarlina, Mukarlina; Rahmawati, Rahmawati; Saputra, Firman; Turnip, Masnur; Indriani, Ade
Jurnal Pengabdi Vol 8, No 1 (2025): April 2025
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jplp2km.v8i1.66091

Abstract

Abstrak: Desa Sungai Kakap merupakan desa yang terletak di Kecamatan Sungai Kakap, Kabupaten Kubu Raya. Kelapa merupakan satu di antara potensi hasil perkebunan di Desa Sungai Kakap yang belum banyak dimanfaatkan. Hasil panen kelapa yang ditanam hampir di sekitaran rumah warga saat ini masih sebatas dijual dalam bentuk butiran kelapa muda dan hanya dikonsumsi pribadi. Upaya peningkatan nilai jual kelapa dapat dilakukan dengan mengolah menjadi Virgin Coconut Oil  (VCO). Pengabdian kepada masyarakat ini dilakukan bertujuan memberikan sosialisasi kepada masyarakat tentang pemanfaatan kelapa menjadi Virgin Coconut Oil  (VCO). Metode yang digunakan pada pelatihan meliputi penyampaian teori dan praktek pembuatan VCO. Peserta pelatihan adalah ibu-ibu PKK di Desa Sungai Kakap, Kabupaten Kubu Raya, Kalimantan Barat. Evaluasi kegiatan PKM dilakukan dengan metode kuisioner yang diberikan pada akhir pelatihan.  Kata Kunci:  Desa Sungai Kakap, Pelatihan, VCO
Perkecambahan Biji Buah Naga Merah (Hylocereus polyrhizus) dengan Variasi Konsentrasi Ekstrak Tomat (Solanum lycopersicum L.) dan Lama Waktu Perendaman Indrasari, Dita; Zakiah, Zulfa; Gusmalawati, Dwi
Produksi Tanaman Vol. 11 No. 12 (2023): Desember
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.protan.2023.011.12.07

Abstract

Buah naga dapat diperbanyak secara generatif menggunakan biji. Salah satu cara yang dapat digunakan untuk mempercepat proses perkecambahan adalah dengan merendam biji menggunakan zat pengatur tumbuh (zpt). Ekstrak tomat merupakan zpt yang dapat mempercepat perkecambahan biji. Tujuan penelitian ini adalah untuk mengetahui pengaruh konsentrasi ekstrak tomat dan lama waktu perendaman terhadap perkecambahan biji buah naga. Bahan yang digunakan pada penelitian ini, yaitu aquades, biji buah naga (Hylocereus polyrhizus), ekstrak buah tomat (Solanum lycopersicum L. var. intan). Penelitian ini merupakan penelitian eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) faktorial yang terdiri dari dua faktor, yaitu konsentrasi ekstrak tomat dan waktu perendaman. Faktor pertama, konsentrasi ekstrak tomat (T), yaitu terdiri dari Kontrol (T0), 4% (T1), 6% (T2), 8% (T3), dan 10% (T4). Faktor kedua lama perendaman (P), yaitu Kontrol (P0), 6 Jam (P1), 12 Jam (P2), 24 Jam (P3), 48 Jam (P4). Penelitian dilakukan di Laboratorium Biologi Umum, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Tanjungpura, Pontianak. Penelitian dilakukan pada bulan Agustus hingga September 2022. Hasil penelitian menunjukkan bahwa waktu perendaman 48 jam merupakan waktu terbaik pada parameter persentase perkecambahan (74,8%), waktu muncul kecambah (3,96 hst), dan panjang radikula (0,29 cm). Konsentrasi ekstrak tomat 10% merupakan konsentrasi terbaik pada parameter waktu muncul kecambah. Kombinasi konsentrasi ekstrak tomat dan lama waktu perendaman memberikan hasil tidak berpengaruh nyata terhadap semua parameter pengamatan.
Leaf morphology and anatomy of Rhizophora apiculata Blume. in Different Zonation of Sukadana Mangrove Tourism Fitri, Anjelina; Zakiah, Zulfa; Rafdinal, Rafdinal
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.8582

Abstract

Rhizophora apiculata is a mangrove species that lives in coastal areas. Differences in zoning will affect the growth of mangroves both morphologically and anatomically. This study aims to determine the morphological and anatomical characteristics of R. apiculata leaves in different zones. The research method used is descriptive method. Zone A is the zone near the sea water and zone B is the zone away from the water. Morphological observations were made directly, while anatomical observations were made through making preparations with the embedding method. Observations of leaf area parameters of R. apiculata in zone A showed a larger leaf area (83.11 ± 6.07cm2) compared to zone B (63.95 ± 8.61cm2). R. apiculata leaves in zone B had a higher number of stomata (24.42±5.60mm2) and stomatal density (124.43±28.43 cells/mm2) than zone A, which had a higher number of stomata (17.44±5.99mm2) and stomatal density (88.87±30.44 cells/mm2). Mesophyll tissue thickness parameters showed no significant difference between zone A and zone B. Parameters of salt glands showed significant differences between zone A and zone B. The results of the study, it can be concluded that differences in mangrove zones provide different responses and adaptations to the morphology and anatomy of R. apiculata leaves.
The Growth of Lemna Minor and Pistia Stratiotes in Batulayang Landfill’s Leachate Perdana, Aurendyo Yuscel; Zakiah, Zulfa; Rusmiyanto, Elvi
Jurnal Biologi Tropis Vol. 25 No. 4 (2025): Oktober-Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4.9513

Abstract

Lemna minor and Pistia stratiotes are plants that can be used to degrade haevy metal pollutants iron (Fe) and lead (Pb) present in aquatic environments and can imprave water quality. The purporse of this study was to determine the best combination between L. minor and P. stratiotes in improving leachate water quality. This study uses a Completely Randomized Design (CRD) with five treatment levels with 3 replications each. The data obtained were then analyzed using descriptive.The results showed that the best plant combination in improving leachate water quality and capable of absorbing heavy metals iron and lead was L1P3 with a percentage reduction of heavy metals iron and lead of 98,2% and 85 % respectively as a result L. minor and P. stratiotes after treatment on Batu Layang Landfill leachate experienced an increase in biomass and chlorophyll content, as well as being able to reduce lead and iron levels. The results of this study are in line with Minister of Environment Regulation of the Republic of Indonesia Number 59 of 2016 concerning quality standards for leachate from business premises or final disposal sites.
In Vitro Shoot Culture Growth of Pontianak Orange (Citrus nobilis var. microcarpa) with the Addition of Naphthalene Acetic Acid and 6-Benzyl Amino Purine Nissa, Zoelva Zakiatun; Zakiah, Zulfa; Turnip, Masnur
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6344

Abstract

Pontianak siam orange (Citrus nobilis var. microcarpa) is an important agricultural commodity unique to Sambas Regency, West Kalimantan. The demand for Pontianak siam oranges has been steadily increasing over the years, yet the production of Pontianak oranges has not been able to meet the domestic demand. The limited availability of superior seedlings is one of the reasons for this situation. Therefore, efforts are needed to fulfill the demand for Pontianak siam oranges by producing a large quantity of superior seedlings in a short period. One of the strategies that can be employed is in vitro propagation. This research aims to observe the growth of Pontianak Siamese orange shoot explants after administration of NAA (Naphthalene Acetic Acid) and BAP (6-Benzyl Amino Purine). This research was conducted from January to June 2023 at the Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University. The study employed a Completely Randomized Design (CRD) with two treatment factors namely NAA concentrations with 4 levels (0; 0.25; 0.5; 0.75 mg/L), and BAP concentrations with 5 levels (0; 0.5; 1; 1.5; 2 mg/L). Parameters observed were the emergence time of shoot, shoot number, height of shoot, and the number of leaves. The results showed that single NAA, single BAP, and combination treatment of NAA and BAP significantly affected all growth parameters. Treatment with the addition of 0.25 mg/L NAA and 1 mg/L BAP (A1B2) was the treatment with the fastest shoot emergence time (2.80 days after planting), the largest number of shoots (2.60 shoots)the tallest shoots (1.50 cm), , and the greatest number of leaves (2.60 leaves).
Induksi Kalus dari Hipokotil Belimbing Merah (Baccaurea angulata) dengan Penambahan 2,4-D (Dichlorophenoxy Acetic Acid) dan BAP (6-Benzyl Amino Purin) Teresia, Novi; Zakiah, Zulfa; Turnip, Masnur
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6387

Abstract

Red starfruit is one of the endemic plants of Kalimantan which produces secondary metabolites which has been empirically used as antioxidant, antibacterial, and anticancer. This plant has a long reproductive period and seedless fruit is often found during the fruiting season, which is a problem for the sustainability of the red starfruit in nature. Conservation of red star fruit for propagation and production of secondary metabolites can be done in vitro through callus culture. This research aims to determine the effect of adding the growth regulators dichlorophenoxy acetic acid (2,4-D) and 6-benzyl amino purine (BAP) on callus induction time, percentage of callus forming explants, callus color and texture, and callus growth. This research used a Completely Randomized Design (CRD) with a factorial pattern consisting of two treatment factors, namely 2,4-D (0; 0.25; 0.5; 0.75; 1 ppm) and BAP (0; 0.25; 0.5; 0.75; 1 ppm). The percentage of explants forming callus (%), the time the callus appeared (days after planting), the color and texture of the callus, the wet weight and dry weight of the callus (g) were research observation parameters.The results showed that the single factor 2,4-D, single factor BAP, and the interaction of 2,4-D and BAP had a significant effect to callus appearance time. Single administration of 2,4-D and BAP had a significant effect on callus dry weight. The color variations of the callus produced are white, green and brown with a compact texture. The fastest callus emergence time was at 11.00 days after planting (DAP), namely in the combination treatment of 1 ppm 2,4-D and 0.75 ppm BAP, single treatment 0.75 ppm and 1 ppm 2,4- D. The highest average dry weight of 0.024 g was obtained in the single treatment of 0.75 ppm BAP.
Induction of Somatic Embryogenesis of Pontianak Siamese Orange Cotyledon Cultures on Murashige Skoog Media with the Addition of 2,4-D and Kinetin Zakiah, Zulfa; Turnip, Masnur
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6721

Abstract

Conventional citrus propagation has weaknesses, one of which is susceptible to CVPD(Citrus Vein Phloem Degeneration) and other diseases which are the main cause of the decline in population and production of pontianak siam orange plants in West Kalimantan. One alternative to in vitro propagation of citrus seedlings is the induction of somatic embryogenesis. The purpose of the study was to obtain the best combination of 2,4-D and kinetin concentrations for callus induction and somatic embryo induction from Pontianak siam orange cotyledon explants, observing the developmental phase (stage) of embryos formed. The implementation of the study used a factorial completely randomized design (CRD) consisting of 2 factors. The first factor is the concentration of 2,4-D consisting of 4 concentration levels, namely 0; 0.5; 1; 1.5; 2 mg/L. The second factor is kinetin concentration consisting of 4 concentration levels, namely 0; 0.5; 1; 1.5; 2 mg/L.The observation parameters include the time of callus emergence (hst), the percentage of explants forming callus (%), the texture and color of the callus, observing the stage of the embryo found. The results showed that 72% of treatments were able to induce callus from pontianak siam orange cotyledons with an average callus appearance at 7 hst. The callus formed is compact and crumbly with varied callus colors, namely white, yellowish, greenish and brownish. The best treatment that produces embryogenic callus is the combination of D0.5K0.5; D1K1; D0.5K1.5; D1.5K1.5 and D2K2 with the characteristics of crumbly callus and yellowish and brownish colors. The results of microscopic observations found that the somatic embryo phase formed was the pro-embryo phase.