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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis The Journal of Experimental Life Sciences (JELS) Journal of Food and Life Sciences Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Pembangunan dan Alam Lestari JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Perikanan Universitas Gadjah Mada Samakia: Jurnal Ilmu Perikanan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Journal of Aquaculture and Fish Health Jurnal Lemuru : Jurnal Ilmu Perikanan dan Kelautan Indonesia Jurnal Perikanan Pantura Berkala Penelitian Hayati Fish Scientiae : Jurnal Ilmu-Ilmu Perikanan dan Kelautan Prosiding Seminar Nasional Pengabdian Kepada Masyarakat KOPEMAS Genbinesia Journal of Biology Water and Marine Pollution Journal: PoluSea
Yuni Kilawati
Fakultas Perikanan Dan Ilmu Kelautan, Universitas Brawijaya, Malang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam Valen, Fitri Sil; Widodo, Maheno Sri; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1080.646 KB) | DOI: 10.21776/ub.jels.2019.009.01.07

Abstract

In general, the process of naming several species of fish including species from the genus Mystacoleucus is still carried out based on morphological characters, which are often found in almost the same morphological characters in different fish species. Therefore, it is necessary to apply a more accurate identification method, which is a DNA-based identification method called DNA barcoding. The purpose of this study was to identify the species of the genus Mystacoleucus on the Sengguruh Dam molecularly based on the Cytochrome Oxidase C Subunit I (COI) gene. A caudal fin was taken on the test fish and preserved in 95% ethanol solution for molecular identification. The results of the identification showed that the sample belonged to the Mystacoleucus marginatus species with Identity values between 99-100% and E-value 0.0. The data obtained showed that from the calculation of genetic distance presented in the form of data matrix and phylogenetic tree reconstruction, there were 2 species that had a far genetic distance from the M. marginatus sample from the Sengguruh Dam namely M. atridorsalis with the furthest genetic distance of 0.1932-0.2595 and M. lepturus with genetic distance between 0.1117-0.1193. However, there are one species that has the closest distance, M. padangensis with genetic distance between 0.0019-0.0038 and identity values up to 99%.Keywords: DNA barcode, Molecular identification, Mystacoleucus, Phylogenetic COI.
Heavy Metal (Pb) and Its Bioaccumulation in Red Algae (Gracilaria sp.) At Kupang Village, Jabon Sub-District, Sidoarjo District Tega, Yatris Rambu; Herawati, Endang Yuli; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1146.597 KB) | DOI: 10.21776/ub.jels.2019.009.02.13

Abstract

Seaweeds have an inherent capacity to absorb heavy metals from marine water. This intrinsic ability allows these organisms to accumulate much amount of heavy metals over time. This study conducted at Kupang Village, Jabon Sub-District, Sidoarjo District, where almost 40% of the area consists of Gracilaria sp. ponds cultured. The purpose of this study was to analyze the Pb heavy metals concentration in water culture and its concentration in holdfast and thallus of Gracilaria sp. In this research, determination of samples in each pond was taken on day 0 (before planting), 20 days (Initial Production) and on day 40th (Post/Harvest). The aquatic parameters include salinity, temperature, acidity (pH), and dissolved oxygen (DO). Quantitative determination of heavy metals on sample using Atomic Absorption Spectrophotometry (AAS). Determinations of heavy metal accumulation in organism using Bioconcentratin factor (BCF) and Translocation factor was used to calculate the Pb heavy metal translocation process from the base to the tip of Gracilaria sp. The results of the study showed the highest Pb concentration found in the second sampling age 20 days, in pond 1 with 7.61 ± 0.18 mg.kg-1, and pond 2 was 5.35 ± 0.09 mg.kg-1. This concentration has not exceeded the threshold value that might have an effect if more than 8.6 mg.kg-1. The highest Pb level at the holdfast of Gracilaria sp. found at age 0 days before planting, which is 3.38 ± 0.23 mg.kg-1 and decreases to post-harvest (age of 40 days) which is 0.84 ± 0.00 mg.kg-1. The Transaction Factor (TF) of Pb heavy metal value from holdfast to thallus is 1,015 thus Gracilaria sp. absorbs heavy metals in high concentrations at the beginning of planting and is able to release it again before harvest time. Keywords: Bioaccumulation, Gracillaria sp., Heavy Metal, Histological, Red Algae.
Expression Virus-Like Particles (VLPs) at Geomembrane and Concret in Asian Pacific Shrimp Culture (Litopenaeus vannamei) Hidayah, Venny Nur; Musa, Muhammad; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 11 No. 3 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.03.06

Abstract

Asian Pacific Shrimp is an invertebrate that the most culture in aquaculture. But the high demand for shrimp makes farmers cultivate a high density. There are several ways to increase the density of shrimp culture, usually called geomembrane plastic and concrete ponds. The ponds are located in the Laboratory of Brackishwater and Seawater Fisheries, Probolinggo and Lucky Windu, Situbondo. Day of Culture (DOC) on Laboratory of Brackishwater and Seawater Fisheries in Probolinggo pond is 85 days both, while in Lucky Windu Situbondo is 81 days on pond 2, and pond  3B 133 days. The study aimed to evaluate correlations of VLPs towards geomembrane and concrete ponds. The research method has two observations: 1. Periodic observations; nitrite, ammonium, and shrimp were observed; 2. Last observation; sampling water for analysis VLPs was observed. Data of sampling water in the different ponds were then analyzed using Pearson correlation analysis to look for the level of tightness and direction of the relationship. The results have obtained that geomembrane VLPs and concrete VLPs has a very strong relationship, while the type of geomembrane and concrete was close to 1 > 0.5 and the result is 0.90, 0.96, 0.69, and 0.82. Water quality showed pH 8.1 at geomembrane, nitrite 0.2mg.L-1 at geomembrane, and ammonium of 0.44 mg.L-1 at concrete. The pond area in geomembrane pond 1 and 2 was 1600 m2, meanwhile in concrete both is 4300 m2, which mean in concrete ponds more the existence of VLPs is because due to the different pond area factors and indicates from detections of WSSV that the result was positive that makes environment factor in abundances VLPs. But VLPs have no effect on the growth of shrimp vaname during the cultivated process.Keywords: Asian Shrimp Pasific (L. vannamei), Virus-Like Particles (VLPs), Water Quality, and WSSV.
Analysis of Water Quality and Phagocytic Activity (Clarias Sp.) in Aquaponic and Non-Aquaponic Cultivation Systems Punto Apri Sembodo; Asus Maizar Suryanto Hertika; Yuni Kilawati
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.4295

Abstract

Catfish (Clarias sp.) is one of the many fishery biota that has animal protein which is much loved by Indonesian people. One way to develop environmentally friendly catfish cultivation is through the implementation of an aquaponic biofilter system. This research aims to analyze physical, chemical, and biological water quality parameters in catfish cultivation with and without aquaponics, to obtain a description of the non-specific immune system which is analyzed from the conditions of phagocytic activity of catfish cultivated with and without aquaponics. The method used is by taking samples ten times per week by taking water quality parameters as well as blood samples of catfish raised in ponds with and without aquaponics. The analysis used is the unpaired T-test. Based on the results obtained, it was concluded that all parameters showed that the aquaponic cultivation media was better compared to those without aquaponics.
Analysis of Protein Profile and Functional Groups in Biofilms in the Hypersaline Environment Ilham Misbakudin Al Zamzami; Yuni Kilawati; Raudatul Ibdiah; Andi Kurniawan
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4464

Abstract

Halophilic microorganisms include extremophiles that can live and develop in a hypersaline environment. Biofilm, as microecology, consists of a collection of microorganisms attached to a surface, forming an Extracellular Polymeric Substance (EPS) matrix. This study aims to analyze the protein profile and functional groups of biofilms that grow in hypersaline environments. The method used in this study is the SDS PAGE test to diagnose protein profiling and FTIR to analyze biofilm functional groups. The samples used to grow the biofilm came from salt ponds with different NaCl levels, namely 2%, 25%, and 40%. The results showed that each NaCl treatment had different functional groups in the biofilm samples. In the 2% NaCl treatment, there were peaks with solid intensity at the wavenumber 1100 cm-1, indicating the carboxylic acid functional group. In the 25% NaCl treatment, peaks with solid intensity at wavenumber 2250 cm-1 indicate the isocyanate functional group. In the 40% NaCl treatment, there were peaks with solid intensity at wavenumber 860 cm-1, indicating the alkene functional group. The protein analysis for each treatment shows the presence of pyruvate kinase protein in the biofilm with 2% NaCl. The 25% NaCl biofilm sample contained avidin protein, and the 40% NaCl biofilm sample contained tyrosine protein.
Identifikasi dan Analisis Molekuler Hemosianin sebagai Gen Ketahanan Tubuh Udang Vaname (Litopenaeus vannamei) Talitha Amanda; Yuni Kilawati; Maftuch
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.7331

Abstract

Production of white shrimp (Litopenaeus vannamei) tends to decrease every year caused by diseases, one of which is White Feces Disease (WFD). Genetically, monitoring has been carried out on the parent characters of SPF (Specific Pathogen Free) shrimp imported from Hawaii and Florida with low genetic variation. If there is a threat such as disease caused by bacteria and the gene that codes for disease control or the body's resistance gene is missing, it allows the individual to become susceptible to disease. Gene that codes for body resistance in white shrimp is hemocyanin. Hemocyanin gene studies are needed as a first step in controlling disease in white shrimp. Method used is a descriptive method. Procedures carried out are sample collection, detection of WFD, molecular analysis of hemocyanin and data analysis. Results showed that healthy shrimp had hemocyanin while shrimp with WFD had very little or no amount of hemocyanin. Hemocyanin gene profile in healthy shrimp was also analyzed and there were 4 nucleotide change made changes to 4 amino acid. Changes in amino acids affect the stability of hemocyanin and cause polymorphism of hemocyanin. Phylogenetic analysis showed dendogram split into two clades between L. vannamei and P. monodon.
Household-Scale Biodigester Application: Transforming Livestock Waste into a New Source of Income in Kasembon Subdistrict Kilawati, Yuni; Amrillah, Attabik Mukhammad; Maimunah, Yunita; Kartikasari, Dany Primanita; Muttaqin, Adharul
Journal of Innovation and Applied Technology Vol 12, No 1 (2026)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiat.2025.012.01.10

Abstract

Livestock farmers in Kasembon Subdistrict face dual challenges: severe environmental pollution from dumping manure directly into rivers, and economic strain due to high dependence on costly LPG and synthetic fertilizers. This community service program introduces household-scale biodigesters to solve both environmental and economic issues simultaneously. Involving 20 local participants through the Participatory Rural Appraisal (PRA) method, capacity building, and technical assistance, the intervention yielded highly significant outcomes. LPG consumption plummeted from 3.8 to 0.4 cylinders monthly. Behavior completely transformed: previously, 80% of farmers polluted water bodies, whereas today 100% utilize their waste as biodigester feedstock, effectively halting river pollution. Economically, this circular system generates average monthly savings of Rp 206,000 per household by substituting LPG and chemical fertilizers. Participants' technical skills also surged from 1.95 to 4.61. Ultimately, biodigester technology successfully mitigates environmental damage while empowering rural communities toward sustainable energy independence and food security
Pengukuran Kualitas Lingkungan dan Dinamika Bahan Organik di Ranu Klakah, Jawa Timur, Indonesia: Assessing Environment Quality and Dynamics Of Organic Matter in Ranu Klakah, East Java, Indonesia Kilawati, Yuni; Muhammad Nala Khoir
Water and Marine Pollution Journal: PoluSea Vol. 1 No. 2 (2023): Water and Marine Pollution Journal: PoluSea Desember 2023
Publisher : Faculty of Fisheries and Marine Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.polusea.2023.001.02.5

Abstract

Kematian massal ikan merupakan salah satu permasalahan utama yang dihadapi di Ranu Klakah. Hal ini diakibatkan oleh proses kompleks seperti akumulasi bahan organik baik pada dasar perairan maupun kolom air, serta fenomena up welling yang terjadi secara rutin setiap tahun di perairan tersebut. Pada tahap awal penumpukkan bahan organik di dasar perairan mengakibatkan pembentukan lapisan anaerobik yang makin besar dan senyawa beracun seperti H2S dan NH3. Tujuan dari penelitian ini adalah untuk menganalisis status kualitas air, dinamika kadar bahan organik yang disebabkan fenomena up welling, dan hubungan bahan organik dengan parameter kualitas air di Ranu Klakah. Analisis data yang digunakan untuk mengetahui hubungan kualitas air terhadap kelimpahan konsorsium mikroorganisme dengan menggunakan bentuk uji korelasi pearson. Parameter kualitas air yang diukur pada aspek fisika: warna, bau, rasa,temperatur, dan kecerahan/ cuaca, lalu aspek kimia yang diperiksa/diukur langsung di lapangan seperti pH, dissolved oxygen (DO), Biological Oxygen Demand (BOD) dan amonia. Hasil pengukuran parameter bahan organik di Ranu Klakah selama sebelum dan pada saat up welling sangat fluktuatif. Nilai Bahan Organik Ranu Klakah terendah terjadi saat sebelum up welling yaitu pada stasiun outlet dengan nilai bahan organik 3,03% nilai terendah pada saat up welling pada stasiun Inlet dengan nilai bahan organik 7,31%. Hasil analisis hubungan beberapa kualitas air dengan bahan organik pada Ranu Klakah yaitu berpengaruh nyata. Hasil pengukuran pada parameter kualitas air suhu yaitu antara 27°C – 28 °C, nilai pH pada perairan yaitu 7, DO pada perairan antara 11,8 mg/L – 13,3 mg/L, nilai BOD antara 1,8 mg/L – 3,1 mg/L, konsentrasi amonia pada perairan 0,15, konsentrasi nitrat yaitu 0 mg/l dan konsentrasi nitrit diantara 25-50 mg/l.   The problem in Ranu Klakah is the mass death of fish caused by a complex process due to the accumulation of organic matter on the bottom of the water and the water column. This problem results from the phenomenon of upwelling that occurs routinely every year in these waters. In the early stages, due to the build-up of organic matter on the bottom of the water, a more extensive anaerobic layer is formed, followed by toxic compounds such as H2S and NH3. This study aims to analyze the status of water quality and determine the dynamics of organic matter levels caused by the phenomenon of upwelling and the relationship of organic matter with water quality parameters in Ranu Klakah. Research analysis to determine the relationship of water quality to the abundance of microorganism consortiums using the Pearson correlation test form. Water quality parameters are measured in physical aspects: colour, smell, taste, temperature, and brightness/weather, then chemical aspects that are examined/measured directly in the field, such as pH, dissolved oxygen (DO), Biological Oxygen Demand (BOD) and ammonia. The results of measuring organic matter parameters in Ranu Klakah before and during upwelling fluctuate. Ranu Klakah Organic Material value: The lowest value before upwelling is at the outlet station, with an organic matter value of 3.03%. The lowest value at the upwelling at the Inlet station is with an organic matter value of 7.31%. The analysis results of the relationship between some water quality and organic matter in Ranu Klakah have a natural effect. The measurement results on temperature water quality parameters are between 27 °C – 28 °C, pH values in waters are 7, DO in waters between 11.8 mg / L - 13.3 mg / L, BOD values between 1.8 mg / L - 3.1 mg / L, ammonia concentrations in waters 0.15, nitrate concentrations are 0 mg / l and nitrite concentrations between 25-50 mg / l.
Co-Authors Abdul Rahem Faqih Adharul Muttaqin Adhitya Bhawiyuga Agoes Soeprijanto, Agoes Aisyah, Diana Andi Kurniawan Anggita Noer Laily Aprillia Mifthakun Ni’mah Mifthakun Ni’mah Ni’mah Arning W. Ekawati Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Attabik Muhammad Amrillah Attabik Mukhammad Amrillah Ayu Winna Ramadhani Dany Primanita Kartikasari Dany Primanita Kartikasari Dany Primanita Primanita Kartikasari Devi Rahmania Nureka Wasti Dewi Wulandari Diana Aisyah Diana Arfiati Dini Anggraini Endang Yuli Herawati Fahma, Yunita Rifda Alano Fitri Sil Valen Gumelar, Agum Bayu Hafish Gigih Purnama Happy Nursyam Haryono, M. Gandri Hidayah, Venny Nur Huda, Alif Raditya Amirul Ilham Afandy Afandy Ilham Misbakudin Al Zamzami Islamy, R Adharyan Isroni, Wahyu Iswati Iswati Luthfiyah, Saidah Maftuch Maftuch Maftuch Maftuch Maftuch Maheno Sri Widodo, Maheno Sri Marwulan Marwulan Moh. Awaludin Adam Mohamad Fadjar MOHAMAD FADJAR Mohamad Fadjar Mohammad Mahmudi Muhammad Musa Muhammad Nala Khoir Mulyanto, Febri Dwi Murni, Lini Nadiah Nurandi Novalina Serdiati Nurul Mutmainnah, Nurul Pramudia, Zulkisam Punto Apri Sembodo Punto Apri Sembodo Putri, Ardyah R.I. R Adharyan Islamy Raudatul Ibdiah Rikianto, Dimas Adetia Rizqa, Vylzah Sholikah, Lik Anatus Sisca Fajriani Siti Nur Tahirah Sri Andayani Sri Widyarti Subarijanti, Herwati Umi Sugiarto, Kevin Pratama Suliswanto, Suliswanto Susanti, Yogita Ayu Dwi Syams, Muhammad Nafar Amani Talitha Amanda Veryl Hasan Win Darmanto Yatris Rambu Tega Yenny Risjani Yuliezar Perwira Dara, Yuliezar Perwira Yunita Maimunah Zahrotur Rosyidah Zamzami, Ilham Misbakudin Al