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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis The Journal of Experimental Life Sciences (JELS) Journal of Food and Life Sciences Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Pembangunan dan Alam Lestari JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Perikanan Universitas Gadjah Mada Samakia: Jurnal Ilmu Perikanan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Journal of Aquaculture and Fish Health Jurnal Lemuru : Jurnal Ilmu Perikanan dan Kelautan Indonesia Jurnal Perikanan Pantura Berkala Penelitian Hayati Fish Scientiae : Jurnal Ilmu-Ilmu Perikanan dan Kelautan Prosiding Seminar Nasional Pengabdian Kepada Masyarakat KOPEMAS Genbinesia Journal of Biology
Yuni Kilawati
Fakultas Perikanan Dan Ilmu Kelautan, Universitas Brawijaya, Malang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Fraction and Antibacterial Activity of Bitter Melons (Momordica charantia) in Koi Fish (Cypinus carpio) Infected with Aeromonas salmonicida Bacteria and its Effect on Gills Histopathology Nadiah Nurandi; Sri Andayani; Yuni Kilawati
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9338

Abstract

Koi fish (Cyprinus carpio) is a type of freshwater ornamental fish with significant economic value, attracting widespread public interest due to its vibrant colors and agile movements. Therefore, this study introduces a novel approach by evaluating the antibacterial activity of bitter melon extract fraction (Momordica charantia) against A. salmonicida infection in koi fish and its impact on gill histopathology. The novelty of this study lies in the exploration of bitter melon extract fraction as a natural, eco-friendly antibacterial alternative to synthetic antibiotics in aquaculture. This research is crucial as it addresses the growing global concern over antibiotic resistance and the urgent need for sustainable, natural solutions in fish health management. Antibacterial tests were conducted to assess the ability of the bitter melon extract to inhibit bacterial growth, while histopathological analysis aimed to observe the specific changes in gill tissues of infected koi fish. The results revealed that the bitter melon extract fraction significantly reduced bacterial count and improved gill tissue integrity. Histopathological improvements were observed, including reduced hyperplasia, lamellar fusion, edema, and necrosis, indicating tissue recovery. This finding highlights the potential of bitter melon extract fraction as a natural antibacterial agent for controlling bacterial infections in fish, offering an alternative to chemical antibiotics. Further research is needed to determine the optimal dosage and to explore its antibacterial mechanism, ensuring its safe and effective application in aquaculture.
Analysis of Microbial Abundances in Biofilms and Water in Hypersaline Environments with Different NaCl Levels Zamzami, Ilham Misbakudin Al; Kilawati, Yuni; Pramudia, Zulkisam; Susanti, Yogita Ayu Dwi; Kurniawan, Andi
The Journal of Experimental Life Science Vol. 13 No. 2 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.02.07

Abstract

Biofilm is the dominant habitat for aquatic microbes that can grow in various aquatic conditions, including environments with extreme conditions. One of the extreme conditions found in aquatic ecosystems is a hypersaline environment. As part of aquatic biological resources, microbes can also live in this environment. However, although it is the predominant habitat of microbes, biofilms in hypersaline environments have yet to be widely explored. Understanding biofilms in hypersaline environments, especially salt ponds, will increase knowledge about microbial biofilms. The knowledge can open opportunities for using microbes as aquatic resources, such as aquaculture or environmental biotechnology. This study analyzed microbial abundance in biofilms and water in hypersaline environments. The water is from salt ponds on the South Coast of Malang Regency, Indonesia, with different salinity levels (2%, 25%, and 40%). Microbial abundance was analyzed using the culture method using several culture media (Modified Nutrient Agar, Modified Total Plate Agar, Mannitol Salt Agar, and Casein Medium Agar). The substrate for biofilm growth is HDPE, often used as a bottom coating for salt ponds and shrimp ponds. Moreover, this study also analyzed water quality parameters (pH, dissolved oxygen, turbidity, conductivity, total dissolved solids, temperature) during the biofilms' forming process. The results of this study indicate that the abundance of good microbes that grow in the biofilm or the water around the biofilm is strongly influenced by the type of media used for culturing. The main composition of the most influential media is peptone. The density of microbes in the biofilm is hundreds to thousands of times higher than that in the surrounding water. The results of this study also show that the presence of microbes in both water and biofilm affects the water quality properties in hypersaline environments. Keywords: aquatic ecosystem, biofilm, hypersaline, microbial ecology.
The Antigenotoxic Activity of Brown Seaweed (Sargassum sp.) Extract Against Total Erythrocyte and Micronuclei of Tilapia Oreochromis niloticus Exposed by Methomyl-Base Pesticide Kilawati, Yuni; Islamy, R Adharyan
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1130.638 KB) | DOI: 10.21776/ub.jels.2019.009.03.11

Abstract

Pesticides are widely applied in the agriculture sector to protect crops and pest control. The poisonous substance of pesticides will affect all of the organisms, either target and non-target organisms. Fish can play the role of an indicator of genotoxic presence in aquatic environments. Polysaccharide extracts from sargassum have promising anti-genotoxic potential. This study aimed to analyze the anti-genotoxic activity of brown seaweed (Sargassum polycystum) methanol extract against erythrocyte and micronuclei of tilapia exposed by methomyl-base pesticide. Brown seaweed (Sargassum sp.) purchased from farmers in Sumenep Regency, Madura, East Java, then macerated using methanol 1: 3 (w / v) for 3x24 hours at room temperature. The phytochemical screening was including flavonoids, alkaloids, triterpenes, saponins, and tannins. Tilapia fish (TL ± 9-12cm) purchased from the Technical Application Unit of Freshwater Fish (UPT Perikanan Air Tawar), Sumberpasir, Malang, East Java. The result of this study showed that exposure of methomyl-based pesticides in the concentration of 4.015 ppm indicates the formation of micronuclei of 318.33 ‰. The increased concentration of extract treatment is directly proportional to the decrease of micronuclei. It means that sargassum extract can reduce the genotoxic effect on exposed tilapia by methomyl-based pesticides. The best concentration of Sargassum sp. extract that can reduce genotoxic was D (200 ppm). Keywords: Antigenotoxic, Extract, Methomyl, Pesticides, Sargassum sp., Tilapia.
Accumulation of Cd, Cu, Cr, and Pb in Whiteleg Shrimp and Pond Environment of Traditional Aquaculture Systems in Sidoarjo, Indonesia Sugiarto, Kevin Pratama; Kilawati, Yuni; Mahmudi, Mohammad
Jurnal Perikanan Universitas Gadjah Mada Vol 27, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.106352

Abstract

This study investigates the accumulation of heavy metals cadmium (Cd), copper (Cu), chromium (Cr), and lead (Pb) in the water, sediment, and body parts of whiteleg shrimp (Litopenaeus vannamei) cultured in traditional aquaculture ponds in Sidoarjo, Indonesia. The ICP-AES method showed that heavy metals were more concentrated in sediment than in water, particularly Pb and Cd. Cd levels in water and Pb levels in sediment exceeded national and international quality standards. In shrimp tissue, the highest accumulation occurred in the cephalothorax, especially for Cu, although all detected levels remained within the safety limits set by Indonesian standards. Monitoring heavy metals in the aquaculture environment and shrimp is essential, as these contaminants can bioaccumulate and pose health risks to aquatic organisms and humans throughout the food chain. Water quality parameters were generally suitable for shrimp farming, but low pH values may increase heavy metal solubility and bioaccumulation risks. These findings highlight the need for regular monitoring and better environmental management to ensure the sustainability and safety of traditional shrimp aquaculture systems.
Community Empowerment Strategy of Pondok Agung, Kasembon Sub-district through Biogas Technology to Reduce Cattle Waste Pollution in Water Maimunah, Yunita; Kilawati, Yuni; Muttaqin, Adharul; Amrillah, Attabik Mukhammad; Kartikasari, Dany Primanita
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 8 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i8.9544

Abstract

The community-based biogas project in Kasembon sub-district focuses on utilising livestock manure as a renewable energy source and organic fertiliser producer to improve community welfare and reduce negative environmental impacts. Through an Asset-Based Community Development (ABCD) approach, the project identifies local assets, such as land and manure, and empowers the local community to maintain the biogas system. The biogas production process produces environmentally friendly energy and reduces dependence on LPG. In addition, the organic fertiliser made from biogas waste can be utilised in agriculture to improve productivity and soil health. The implementation of ABCD encourages collaboration between the community, government, and university, and creates new economic opportunities by selling organic fertiliser. However, challenges such as dependence on external assistance, high initial capital requirements, and technical limitations hinder long-term sustainability.
Phytochemical profile, antioxidant, and antibacterial activities of Ipomoeae pes-caprae from Pantai Talang Siring Pamekasan, Madura: A promising source of natural bioactive compounds Islamy, R Adharyan; Hasan, Veryl; Serdiati, Novalina; Kilawati, Yuni; Valen, Fitri Sil; Mutmainnah, Nurul
Genbinesia Journal of Biology Vol. 3 No. 2 (2024): March 2024
Publisher : Generasi Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55655/genbinesia.v3i2.49

Abstract

This research investigates the phytochemical composition, antioxidant activity, and antibacterial efficacy of Ipomoea pes-caprae extracts collected from Pantai Talang Siring Pamekasan, Madura. Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolic compounds, terpenoids, tannins, and saponins in the extracts, indicating their rich bioactive profile. Antioxidant activity assessed via the DPPH assay demonstrated concentration-dependent scavenging of free radicals, with methanol and ethanol extracts exhibiting superior activity compared to the aqueous extract. Antibacterial assays against Escherichia coli and Staphylococcus aureus revealed significant inhibitory effects, with methanol and ethanol extracts displaying greater potency than the aqueous extract. These findings underscore the potential of Ipomoea pes-caprae as a source of natural antioxidants and antimicrobial agents, warranting further investigation into its pharmacological applications and therapeutic potential.
Co-Authors Abdul Rahem Faqih Adharul Muttaqin Adhitya Bhawiyuga Agoes Soeprijanto, Agoes Agum Bayu Gumelar Alif Raditya Amirul Huda Andi Kurniawan Andi Kurniawan Anggita Noer Laily Aprillia Mifthakun Ni’mah Mifthakun Ni’mah Ni’mah Arning W. Ekawati Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Attabik Muhammad Amrillah Attabik Mukhammad Amrillah Ayu Winna Ramadhani Dany Primanita Kartikasari Dany Primanita Kartikasari Dany Primanita Primanita Kartikasari Devi Rahmania Nureka Wasti Dewi Wulandari Diana Aisyah Diana Aisyah Diana Arfiati Dimas Adetia Rikianto Dini Anggraini Endang Yuli Herawati Fahma, Yunita Rifda Alano Fitri Sil Valen Fitri Sil Valen Hafish Gigih Purnama Happy Nursyam Haryono, M. Gandri Herwati Umi Subarijanti Ilham Afandy Afandy Ilham Misbakudin Al Zamzami Islamy, R Adharyan Isroni, Wahyu Iswati Iswati Lini Murni Maftuch Maftuch Maftuch Maftuch Maftuch Maheno Sri Widodo Maheno Sri Widodo, Maheno Sri Marwulan Marwulan Moh. Awaludin Adam Mohamad Fadjar MOHAMAD FADJAR Mohamad Fadjar Mohammad Mahmudi Muhammad Musa Muhammad Nafar Amani Syams Mulyanto, Febri Dwi Nadiah Nurandi Novalina Serdiati Nurul Mutmainnah, Nurul Pramudia, Zulkisam Punto Apri Sembodo Punto Apri Sembodo Putri, Ardyah R.I. R Adharyan Islamy Raudatul Ibdiah Rizqa, Vylzah Saidah Luthfiyah Sholikah, Lik Anatus Sisca Fajriani Siti Nur Tahirah Sri Andayani Sri Widyarti Sugiarto, Kevin Pratama Suliswanto Suliswanto Susanti, Yogita Ayu Dwi Talitha Amanda Venny Nur Hidayah Veryl Hasan Win Darmanto Yatris Rambu Tega Yenny Risjani Yuliezar Perwira Dara, Yuliezar Perwira Yunita Maimunah Zahrotur Rosyidah Zamzami, Ilham Misbakudin Al