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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis The Journal of Experimental Life Sciences (JELS) Journal of Food and Life Sciences Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Pembangunan dan Alam Lestari JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Perikanan Universitas Gadjah Mada Samakia: Jurnal Ilmu Perikanan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Journal of Aquaculture and Fish Health Jurnal Lemuru : Jurnal Ilmu Perikanan dan Kelautan Indonesia Jurnal Perikanan Pantura Berkala Penelitian Hayati Fish Scientiae : Jurnal Ilmu-Ilmu Perikanan dan Kelautan Prosiding Seminar Nasional Pengabdian Kepada Masyarakat KOPEMAS Genbinesia Journal of Biology

Yuni Kilawati

Fakultas Perikanan Dan Ilmu Kelautan, Universitas Brawijaya, Malang

Author-ID : 437582

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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The Effect of Electroporation Method towards the Motility and Viability of Java Barb Fish (Puntius javanicus) Sperm Rikianto, Dimas Adetia; Soeprijanto, Agoes; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Graduate School, Universitas Brawijaya

Electric shock treatment of Java Barb Fish (Puntius javanicus) sperm using electroporation method on sperm as transfer gen (Sperm Mediated Gen Transfer) has not been implemented in Indonesia. This study was conducted to know the effect of electric shock using gene pulser at different voltage level toward motility and viability of Java barb fish sperm. This research was conducted at Fish Breeding Laboratory, Faculty of Marine and Fisheries and LSIH of Brawijaya University-Malang in May 2017. The trial design used Complete Randomized Design with 3 different treatments and 1 control which each treatment repeated 3 times. The treatments used the Gene pulser with 3 different voltages: A (20 V), B (30 V), C (40 V). The result showed that the electric shock treatment with different voltages level affected motility and viability of Java barb fish sperm. Based on the data analysis used polynomial orthogonal, a linear-form of the relationship among the treatments in the form of equation was found (Puntius javanicus) with R2=0.9815 and equation y = -40.5x + 130.83 with R2= 0.8626. Based on the result of this research, electroporation for Java barb fish' sperm as gene transfer media should be done with voltage 20 V, pulse number 4 times and pulse length 1 ms. Keywords: electroporation, motility, Puntius javanicus, sperm, viability.

Influence of Different Pulse Length towards Motility and Viability of Ornamental Japanese Carp (Cyprinus carpio Var. Koi) Sperm through Electroporation Method Aisyah, Diana; Soeprijanto, Agoes; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2016.007.01.06

Successful studies about implementation of electroporation method which sperm becomes gene transfer medium for fish transgenesis have reported. Motility and viability are two major factors in successful of electroporation. The objective of the study was to determine optimal pulse length of electroporation towards the motility and viability of Koi fish sperm. The study was conducted at the Central Laboratory of Biological Sciences and Reproduction of Brawijaya University. The data analysis was conducted using completely randomized design. The electroporation method was carried out using voltage of 30 volt with pulse number of 4 times. The pulse length of the electroporation (based on treatment) was 0.5, 1 and 1.5 ms. The result showed the highest percentage of motility was 3.67% in treatment A (pulse length 0.5 ms) with coefficient correlation R2 = 0.9643 and the best percentage of viability was 79.67% in treatment A (pulse length 0.5 ms) with coefficient correlation R2 = 1. Correlation between the pulse length treatment (electroporation) toward motility and viability of Koi fish sperm, in which longer duration of pulse length treatment (electroporation) would decrease the motility and viability percentage of the fish sperm.Keywords: Electroporation, Motility, Pulse length, Sperm, Viability.

Biological Analysis of Blue Swimmer Crab (Portunus pelagicus) from Collectors in Lamongan, Tuban, Pasuruan, and Rembang, Java, Indonesia Murni, Lini; Arfiati, Diana; Kurniawan, Andi; Kilawati, Yuni; Subarijanti, Herwati Umi; Suliswanto, Suliswanto; Syams, Muhammad Nafar Amani; Gumelar, Agum Bayu; Huda, Alif Raditya Amirul
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Graduate School, Universitas Brawijaya

Blue swimmer crab (Portunus pelagicus) can increase the country's incomes due to its high demand of export. This study aims to determine biological analysis on the population of blue swimmer crab in Lamongan, Tuban, Pasuruan, and Rembang. The sampling location was based on the fishing area and the method used survey method from collectors in each location. The blue swimmer crab's biological characteristics which observed are the correlation on wide of carapace and blue swimmer crab weight, sex ratio, and gonad maturity level. The results showed the correlation between the wide and weight of carapace in Lamongan (male: 113.80-122.99 mm and 87.98-121.60 g then female: 108.21-117.27 mm and 71.80-99.74 g), Tuban (male: 113.26-117.74 mm and 89.63-102.76 g g then female: 114.36 mm and 106.51 g), Pasuruan (male: 87.66-94.43 mm and 39.68-49.90 g then female: 101.94-110.89 mm and 66.98-82.89 g), and Rembang (male: 120.30-126.69 mm and 93.53-118.43 g then female: 125.30-132.13 mm and 134.42-157.47 g); sex ratio (male: female) in Lamongan (1.4: 1), Tuban (1: 1.5), Pasuruan (2.8: 1), and Rembang (1: 1.46); and also gonad maturity level in Lamongan (dominant male at level III was 30.29%, female at level II and III was 30.40%), Tuban (dominant male at level II was 41.93%, female at level III was 33.33%), Pasuruan (dominant male at level III was 25.79%, female at level III was 48.10%), and Rembang (dominant male at level II was 32.35%, female at level II was 33.33%). These results indicate that the total of Portunus pelagicus which was being caught had fulfilled the standards rules by No.1/PERMEN-KP/2015. However, it requires continuous direct monitoring and counseling to maintain the stock of blue swimmer crab resources. Keywords: Blue swimmer crab (Portunus pelagicus), gonad maturity level, sex ratio, wide and weight.

Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam Valen, Fitri Sil; Widodo, Maheno Sri; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Graduate School, Universitas Brawijaya

In general, the process of naming several species of fish including species from the genus Mystacoleucus is still carried out based on morphological characters, which are often found in almost the same morphological characters in different fish species. Therefore, it is necessary to apply a more accurate identification method, which is a DNA-based identification method called DNA barcoding. The purpose of this study was to identify the species of the genus Mystacoleucus on the Sengguruh Dam molecularly based on the Cytochrome Oxidase C Subunit I (COI) gene. A caudal fin was taken on the test fish and preserved in 95% ethanol solution for molecular identification. The results of the identification showed that the sample belonged to the Mystacoleucus marginatus species with Identity values between 99-100% and E-value 0.0. The data obtained showed that from the calculation of genetic distance presented in the form of data matrix and phylogenetic tree reconstruction, there were 2 species that had a far genetic distance from the M. marginatus sample from the Sengguruh Dam namely M. atridorsalis with the furthest genetic distance of 0.1932-0.2595 and M. lepturus with genetic distance between 0.1117-0.1193. However, there are one species that has the closest distance, M. padangensis with genetic distance between 0.0019-0.0038 and identity values up to 99%.Keywords: DNA barcode, Molecular identification, Mystacoleucus, Phylogenetic COI.

Heavy Metal (Pb) and Its Bioaccumulation in Red Algae (Gracilaria sp.) At Kupang Village, Jabon Sub-District, Sidoarjo District Tega, Yatris Rambu; Herawati, Endang Yuli; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

Seaweeds have an inherent capacity to absorb heavy metals from marine water. This intrinsic ability allows these organisms to accumulate much amount of heavy metals over time. This study conducted at Kupang Village, Jabon Sub-District, Sidoarjo District, where almost 40% of the area consists of Gracilaria sp. ponds cultured. The purpose of this study was to analyze the Pb heavy metals concentration in water culture and its concentration in holdfast and thallus of Gracilaria sp. In this research, determination of samples in each pond was taken on day 0 (before planting), 20 days (Initial Production) and on day 40th (Post/Harvest). The aquatic parameters include salinity, temperature, acidity (pH), and dissolved oxygen (DO). Quantitative determination of heavy metals on sample using Atomic Absorption Spectrophotometry (AAS). Determinations of heavy metal accumulation in organism using Bioconcentratin factor (BCF) and Translocation factor was used to calculate the Pb heavy metal translocation process from the base to the tip of Gracilaria sp. The results of the study showed the highest Pb concentration found in the second sampling age 20 days, in pond 1 with 7.61 ± 0.18 mg.kg-1, and pond 2 was 5.35 ± 0.09 mg.kg-1. This concentration has not exceeded the threshold value that might have an effect if more than 8.6 mg.kg-1. The highest Pb level at the holdfast of Gracilaria sp. found at age 0 days before planting, which is 3.38 ± 0.23 mg.kg-1 and decreases to post-harvest (age of 40 days) which is 0.84 ± 0.00 mg.kg-1. The Transaction Factor (TF) of Pb heavy metal value from holdfast to thallus is 1,015 thus Gracilaria sp. absorbs heavy metals in high concentrations at the beginning of planting and is able to release it again before harvest time. Keywords: Bioaccumulation, Gracillaria sp., Heavy Metal, Histological, Red Algae.

Expression Virus-Like Particles (VLPs) at Geomembrane and Concret in Asian Pacific Shrimp Culture (Litopenaeus vannamei) Hidayah, Venny Nur; Musa, Muhammad; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 11 No. 3 (2021)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2021.011.03.06

Asian Pacific Shrimp is an invertebrate that the most culture in aquaculture. But the high demand for shrimp makes farmers cultivate a high density. There are several ways to increase the density of shrimp culture, usually called geomembrane plastic and concrete ponds. The ponds are located in the Laboratory of Brackishwater and Seawater Fisheries, Probolinggo and Lucky Windu, Situbondo. Day of Culture (DOC) on Laboratory of Brackishwater and Seawater Fisheries in Probolinggo pond is 85 days both, while in Lucky Windu Situbondo is 81 days on pond 2, and pondÂ 3B 133 days. The study aimed to evaluate correlations of VLPs towards geomembrane and concrete ponds. The research method has two observations: 1. Periodic observations; nitrite, ammonium, and shrimp were observed; 2. Last observation; sampling water for analysis VLPs was observed. Data of sampling water in the different ponds were then analyzed using Pearson correlation analysis to look for the level of tightness and direction of the relationship. The results have obtained that geomembrane VLPs and concrete VLPs has a very strong relationship, while the type of geomembrane and concrete was close to 1 > 0.5 and the result is 0.90, 0.96, 0.69, and 0.82. Water quality showed pH 8.1 at geomembrane, nitrite 0.2mg.L-1 at geomembrane, and ammonium of 0.44 mg.L-1 at concrete. The pond area in geomembrane pond 1 and 2 was 1600 m2, meanwhile in concrete both is 4300 m2, which mean in concrete ponds more the existence of VLPs is because due to the different pond area factors and indicates from detections of WSSV that the result was positive that makes environment factor in abundances VLPs. But VLPs have no effect on the growth of shrimp vaname during the cultivated process.Keywords: Asian Shrimp Pasific (L. vannamei), Virus-Like Particles (VLPs), Water Quality, and WSSV.

Co-Authors Abdul Rahem Faqih Adharul Muttaqin Adhitya Bhawiyuga Agoes Soeprijanto, Agoes Aisyah, Diana Amanda, Talitha Andi Kurniawan Anggita Noer Laily Aprillia Mifthakun Ni’mah Mifthakun Ni’mah Ni’mah Arning W. Ekawati Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Attabik Muhammad Amrillah Attabik Mukhammad Amrillah Ayu Winna Ramadhani Dany Primanita Kartikasari Dany Primanita Kartikasari Dany Primanita Primanita Kartikasari Devi Rahmania Nureka Wasti Dewi Wulandari Diana Aisyah Diana Arfiati Dini Anggraini Endang Yuli Herawati Fahma, Yunita Rifda Alano Fitri Sil Valen Gumelar, Agum Bayu Hafish Gigih Purnama Happy Nursyam Haryono, M. Gandri Hidayah, Venny Nur Huda, Alif Raditya Amirul Ibdiah, Raudatul Ilham Afandy Afandy Ilham Misbakudin Al Zamzami Islamy, R Adharyan Isroni, Wahyu Iswati Iswati Luthfiyah, Saidah Maftuch Maftuch Maftuch Maftuch Maftuch Maheno Sri Widodo, Maheno Sri Marwulan Marwulan Moh. Awaludin Adam MOHAMAD FADJAR Mohamad Fadjar Mohamad Fadjar Mohammad Mahmudi Muhammad Musa Mulyanto, Febri Dwi Murni, Lini Nadiah Nurandi Novalina Serdiati Nurul Mutmainnah, Nurul Pramudia, Zulkisam Punto Apri Sembodo Punto Apri Sembodo Putri, Ardyah R.I. R Adharyan Islamy Raudatul Ibdiah Rikianto, Dimas Adetia Rizqa, Vylzah Sembodo, Punto Apri Sholikah, Lik Anatus Sisca Fajriani Siti Nur Tahirah Sri Andayani Sri Widyarti Subarijanti, Herwati Umi Sugiarto, Kevin Pratama Suliswanto, Suliswanto Susanti, Yogita Ayu Dwi Syams, Muhammad Nafar Amani Veryl Hasan Win Darmanto Yatris Rambu Tega Yenny Risjani Yuliezar Perwira Dara, Yuliezar Perwira Yunita Maimunah Zahrotur Rosyidah Zamzami, Ilham Misbakudin Al

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