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Analysis of Water Quality and Phagocytic Activity (Clarias Sp.) in Aquaponic and Non-Aquaponic Cultivation Systems Punto Apri Sembodo; Asus Maizar Suryanto Hertika; Yuni Kilawati
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.4295

Abstract

Catfish (Clarias sp.) is one of the many fishery biota that has animal protein which is much loved by Indonesian people. One way to develop environmentally friendly catfish cultivation is through the implementation of an aquaponic biofilter system. This research aims to analyze physical, chemical, and biological water quality parameters in catfish cultivation with and without aquaponics, to obtain a description of the non-specific immune system which is analyzed from the conditions of phagocytic activity of catfish cultivated with and without aquaponics. The method used is by taking samples ten times per week by taking water quality parameters as well as blood samples of catfish raised in ponds with and without aquaponics. The analysis used is the unpaired T-test. Based on the results obtained, it was concluded that all parameters showed that the aquaponic cultivation media was better compared to those without aquaponics.
Analysis of Protein Profile and Functional Groups in Biofilms in the Hypersaline Environment Ilham Misbakudin Al Zamzami; Yuni Kilawati; Raudatul Ibdiah; Andi Kurniawan
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4464

Abstract

Halophilic microorganisms include extremophiles that can live and develop in a hypersaline environment. Biofilm, as microecology, consists of a collection of microorganisms attached to a surface, forming an Extracellular Polymeric Substance (EPS) matrix. This study aims to analyze the protein profile and functional groups of biofilms that grow in hypersaline environments. The method used in this study is the SDS PAGE test to diagnose protein profiling and FTIR to analyze biofilm functional groups. The samples used to grow the biofilm came from salt ponds with different NaCl levels, namely 2%, 25%, and 40%. The results showed that each NaCl treatment had different functional groups in the biofilm samples. In the 2% NaCl treatment, there were peaks with solid intensity at the wavenumber 1100 cm-1, indicating the carboxylic acid functional group. In the 25% NaCl treatment, peaks with solid intensity at wavenumber 2250 cm-1 indicate the isocyanate functional group. In the 40% NaCl treatment, there were peaks with solid intensity at wavenumber 860 cm-1, indicating the alkene functional group. The protein analysis for each treatment shows the presence of pyruvate kinase protein in the biofilm with 2% NaCl. The 25% NaCl biofilm sample contained avidin protein, and the 40% NaCl biofilm sample contained tyrosine protein.
KOMPOSISI JENIS DAN KELIMPAHAN RELATIF FITOPLANKTON DI RANU KLAKAH, LUMAJANG, JAWA TIMUR: SPECIES COMPOSITION AND RELATIVE ABUNDANCE OF PHYTOPLANKTON IN RANU KLAKAH, LUMAJANG, EAST JAVA Mukhammad Amrillah, Attabik; Kilawati, Yuni; Rizqa, Vylzah
JFMR (Journal of Fisheries and Marine Research) Vol. 7 No. 3 (2023): JFMR on November
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2023.007.03.4

Abstract

Penelitian telah dilaksanakan di Ranu Klakah, Kabupaten Lumajang, Jawa Timur. Tujuan dari penelitian ini adalah untuk mengetahui komposisi jenis dan kelimpahan relatif fitoplankton di perairan Ranu Klakah, Kabupaten Lumajang. Stasiun pengambilan sampel ditentukan pada tiga titik yakni inlet, tengah (kawasan KJA), dan outlet. Pengambilan sampel dilakukan sebanyak 3 kali. Parameter yang diamati meliputi parameter fisika, kimia dan biologi. Parameter fisika dan kimia perairan yang diamati meliputi suhu, kecerahan, pH, DO, nitrat serta orthofosfat. Divisi fitoplankton yang ditemukan memiliki spesies terbanyak adalah Chlorophyta, Chrysophyta, dan Ochrophyta. Tetraspora sp yang termasuk divisi Divisi Chlorophyta memiliki kelimpahan yang paling tinggi jika dibandingkan dengan jenis spesies plankton lainnya. Data kualitas air diperoleh suhu perairan berkisar 27,6 – 28oC, kecerahan peraiaran berkisar 60 cm – 119 cm, nilai pH peraiaran 7, oksigen terlarut berkisar 7 – 7,8 mg/l, nitrat berkisar 0,23 – 1,04 mg/l, sedangkan orthofosfat didapatkan nilai 0,1 mg/l. Berdasarkan hasil penelitian yang telah dilaksanakan dapat disimpulkan bahwa wilayah perairan Ranu Klakah masih tergolong dalam perairan mesotrofik (perairan yang tingkat kesuburan sedang). Berdasarkan dari komposisi jenis dan kelimpahan fitoplankton dapat disimpulkan bahwa keanekaragaman jenis yang dimiliki Ranu Klakah masih tinggi.   The research was carried out in Ranu Klakah, Lumajang Regency, East Java. The purpose of this study was to determine the species composition and relative abundance of phytoplankton in Ranu Klakah, Lumajang Regency. Sampling site were determined at three points, namely inlet, middle (KJA area), and outlet. Sampling was carried out 3 times. Parameters observed included physical, chemical and biological parameters. Physical and chemical parameters of the waters observed included temperature, brightness, pH, DO, nitrate and orthophosphate. Phytoplankton divisions that were found to have the most species were Chlorophyta, Chrysophyta, and Ochrophyta. Tetraspora sp which belongs to the Chlorophyta Division has the highest abundance when compared to other types of plankton species. Water quality data obtained water temperature ranged from 27.6 – 28oC, water brightness ranged from 60 cm – 119 cm, water pH value was 7, dissolved oxygen ranged from 7 – 7.8 mg/l, nitrate ranged from 0.23 – 1.04 mg/l, while orthophosphate obtained a value of 0.1 mg/l. Based on the results of the research that has been carried out, it can be concluded that the waters of Ranu Klakah are still classified as mesotrophic. Based on the species composition and abundance of phytoplankton, it can be concluded that the species diversity of Ranu Klakah is still high.
DIVERSIFIKASI PRODUK HASIL PANEN BUDIDAYA KJA DI RANU KLAKAH UNTUK PENINGKATAN KETAHANAN PANGAN DAN EKONOMI BIRU Kartikasari, Dany Primanita; Kilawati, Yuni; Maimunah, Yunita; Amrillah, Attabik Mukhammad; Muttaqin, Adharul
Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Vol. 4 (2023): PROSIDING SEMINAR NASIONAL PENGABDIAN KEPADA MASYARAKAT - SNPPM2023
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Negeri Jakarta

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Abstract

Abstract Ranu Klakah is located in Tegalrandu Village, Klakah District, Lumajang Regency, and is a center for freshwater fisheries, especially Tilapia fish. Tilapia fish commodities produced in Ranu Klakah have characteristics, including thick flesh and better taste, so the selling price can be higher than production from other areas. The problem that always occurs in Ranu Klakah every year is upwelling which causes tilapia fish to experience mass deaths. On the one hand, this situation is beneficial because apart from the cultivated fish also from Ranu it will be easily harvested in large quantities by the local community. On the other hand, the number of simultaneous harvests causes a buildup of Tilapia stock which will cause prices to drop compared to usual or damage if the fish are not sold immediately. It is also felt that knowledge of marketing methods for fishery products that keep up with current developments is still lacking so the Tilapia commodity from Ranu Klakah can only be enjoyed by the surrounding community. The Brawijaya University Doctoral Community Service Team offers a solution by implementing product diversification aimed at increasing shelf life and selling value which will also directly increase community food security. The activities carried out are in the form of community service through the socialization process, training in making nuggets and chips made from tilapia fish as well as the introduction of marketing systems using IT, mentoring, and evaluation for cultivator groups in Ranu Klakah. Abstrak Ranu Klakah terletak di Desa Tegalrandu Kecamatan Klakah Kabupaten Lumajang, merupakan sentra perikanan tawar terutama ikan Nila. Komoditi ikan Nila yang dihasilkan di Ranu Klakah memiliki kekhasan antara lain berdaging tebal dan lebih enak rasanya, sehingga harga jualnya bisa lebih tinggi dibandingkan produksi dari daerah lain. Permasalahan yang selalu terjadi di Ranu Klakah setiap tahun adalah terjadinya upwelling yang menyebabkan Ikan nila mengalami kematian massal. Di satu sisi keadaan ini menguntungkan karena selain ikan hasil budidaya juga dari Ranu akan dengan mudah dipanen dengan jumlah banyak oleh masyarakat sekitar. Di sisi lain jumlah panen serentak tersebut menyebabkan bertumpuknya stock Ikan Nila yang akan menyebabkan turun harga dibandingkan biasanya atau terjadi kerusakan jika ikan tidak segera terjual. Pengetahuan metode pemasaran hasil perikanan yang mengikuti perkembangan jaman juga dirasakan masih kurang sehingga komoditi Nila dari ranu Klakah hanya bisa dinikmati oleh masyarakat di sekitarnya saja. Tim Doktor Mengabdi Universitas Brawijaya menawarkan solusi dengan menerapkan diversifikasi produk yang bertujuan meningkatkan daya simpan, nilai jual yang secara langsung juga akan meningkatkan ketahanan pangan masyarakat. Kegiatan yang dilaksanakan berbentuk pengabdian kepada masyarakat melalui proses sosialisasi, pelatihan pembuatan nugget dan keripik berbahan dasar ikan nila serta pengenalan sistem pemasaran menggunakan IT, pendampingan dan evaluasi kepada kelompok pembudidaya di Ranu Klakah.
Alleviation Strategies Of Stunting In Infants' Problems Using Tilapia Fish Farming In Sukodono District, Lumajang Regency Kilawati, Yuni; Putri, Ardyah R.I.; Fahma, Yunita Rifda Alano; Dara, Yuliezar Perwira
Journal of Innovation and Applied Technology Vol 10, No 1 (2024)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiat.2024.10.01.007

Abstract

Various programs to fulfill community nutrition by the government in Sukodono District have been actively implemented to alleviate the problem of stunting. Our service activities are based on strategic issues faced in Sukodono District, consisting of education regarding the causes and solutions to the stunting problem and socializing one of the efforts to eradicate stunting. Supported by the Lumajang Regency Government, it has made various efforts to handle and alleviate stunting in Lumajang, so this outreach activity provides insight to the community, especially posyandu cadres, prospective brides, and mothers. This activity aims to help the communities in the Sukodono District, Lumajang Regency, to eradicate the problem of stunting by socializing the tilapia fish farming model and the benefits of tilapia fish. The activity is community service through socialization, which can increase public understanding before and after presenting material on tilapia program activities to prevent stunting.
UPAYA PEMBERDAYAAN LIMBAH ORGANIK DAN PEMANFAATANNYA SEBAGAI SUMBER ENERGI BIOGAS YANG RAMAH LINGKUNGAN Yuni Kilawati; Yunita Maimunah; Adharul Muttaqin; Attabik Muhammad Amrillah; Dany Primanita Kartikasari
Konferensi Nasional Pengabdian Masyarakat (KOPEMAS) #5 2024 Konferensi Nasional Pengabdian Masyarakat (KOPEMAS) #5
Publisher : Konferensi Nasional Pengabdian Masyarakat (KOPEMAS) #5 2024

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Abstract

Kecamatan Kasembon, Kabupaten Malang, memiliki populasi peternak sapi yang menghasilkan limbah signifikan, menimbulkan masalah lingkungan seperti polusi udara, air, dan penyebaran bakteri berbahaya. Untuk mengatasi hal ini, tim Doktor Mengabdi Universitas Brawijaya menerapkan teknologi biogas guna mengolah kotoran sapi menjadi energi alternatif yang mengurangi ketergantungan masyarakat terhadap LPG serta menyediakan pupuk organik. Metode pelaksanaan meliputi survei potensi wilayah, analisis kebutuhan, dan pembangunan reaktor biogas sesuai kondisi setempat. Program ini dilengkapi penyuluhan untuk meningkatkan kesadaran masyarakat akan dampak negatif limbah dan manfaat biogas sebagai energi alternatif. Hasil implementasi menunjukkan bahwa reaktor biogas berhasil memenuhi kebutuhan energi dua rumah tangga dan mengurangi masalah limbah. Kesimpulannya, program ini berkontribusi pada peningkatan kualitas lingkungan, penghematan energi, dan kemandirian energi masyarakat. Partisipasi tokoh masyarakat dan dukungan pihak setempat menunjukkan potensi perluasan penerapan teknologi biogas untuk keberlanjutan lingkungan di Kecamatan Kasembon.
Analysis of Microbial Abundances in Biofilms and Water in Hypersaline Environments with Different NaCl Levels Zamzami, Ilham Misbakudin Al; Kilawati, Yuni; Pramudia, Zulkisam; Susanti, Yogita Ayu Dwi; Kurniawan, Andi
The Journal of Experimental Life Science Vol. 13 No. 2 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.02.07

Abstract

Biofilm is the dominant habitat for aquatic microbes that can grow in various aquatic conditions, including environments with extreme conditions. One of the extreme conditions found in aquatic ecosystems is a hypersaline environment. As part of aquatic biological resources, microbes can also live in this environment. However, although it is the predominant habitat of microbes, biofilms in hypersaline environments have yet to be widely explored. Understanding biofilms in hypersaline environments, especially salt ponds, will increase knowledge about microbial biofilms. The knowledge can open opportunities for using microbes as aquatic resources, such as aquaculture or environmental biotechnology. This study analyzed microbial abundance in biofilms and water in hypersaline environments. The water is from salt ponds on the South Coast of Malang Regency, Indonesia, with different salinity levels (2%, 25%, and 40%). Microbial abundance was analyzed using the culture method using several culture media (Modified Nutrient Agar, Modified Total Plate Agar, Mannitol Salt Agar, and Casein Medium Agar). The substrate for biofilm growth is HDPE, often used as a bottom coating for salt ponds and shrimp ponds. Moreover, this study also analyzed water quality parameters (pH, dissolved oxygen, turbidity, conductivity, total dissolved solids, temperature) during the biofilms' forming process. The results of this study indicate that the abundance of good microbes that grow in the biofilm or the water around the biofilm is strongly influenced by the type of media used for culturing. The main composition of the most influential media is peptone. The density of microbes in the biofilm is hundreds to thousands of times higher than that in the surrounding water. The results of this study also show that the presence of microbes in both water and biofilm affects the water quality properties in hypersaline environments. Keywords: aquatic ecosystem, biofilm, hypersaline, microbial ecology.
The Antigenotoxic Activity of Brown Seaweed (Sargassum sp.) Extract Against Total Erythrocyte and Micronuclei of Tilapia Oreochromis niloticus Exposed by Methomyl-Base Pesticide Kilawati, Yuni; Islamy, R Adharyan
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1130.638 KB) | DOI: 10.21776/ub.jels.2019.009.03.11

Abstract

Pesticides are widely applied in the agriculture sector to protect crops and pest control. The poisonous substance of pesticides will affect all of the organisms, either target and non-target organisms. Fish can play the role of an indicator of genotoxic presence in aquatic environments. Polysaccharide extracts from sargassum have promising anti-genotoxic potential. This study aimed to analyze the anti-genotoxic activity of brown seaweed (Sargassum polycystum) methanol extract against erythrocyte and micronuclei of tilapia exposed by methomyl-base pesticide. Brown seaweed (Sargassum sp.) purchased from farmers in Sumenep Regency, Madura, East Java, then macerated using methanol 1: 3 (w / v) for 3x24 hours at room temperature. The phytochemical screening was including flavonoids, alkaloids, triterpenes, saponins, and tannins. Tilapia fish (TL ± 9-12cm) purchased from the Technical Application Unit of Freshwater Fish (UPT Perikanan Air Tawar), Sumberpasir, Malang, East Java. The result of this study showed that exposure of methomyl-based pesticides in the concentration of 4.015 ppm indicates the formation of micronuclei of 318.33 ‰. The increased concentration of extract treatment is directly proportional to the decrease of micronuclei. It means that sargassum extract can reduce the genotoxic effect on exposed tilapia by methomyl-based pesticides. The best concentration of Sargassum sp. extract that can reduce genotoxic was D (200 ppm). Keywords: Antigenotoxic, Extract, Methomyl, Pesticides, Sargassum sp., Tilapia.
Accumulation of Cd, Cu, Cr, and Pb in Whiteleg Shrimp and Pond Environment of Traditional Aquaculture Systems in Sidoarjo, Indonesia Sugiarto, Kevin Pratama; Kilawati, Yuni; Mahmudi, Mohammad
Jurnal Perikanan Universitas Gadjah Mada Vol 27, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.106352

Abstract

This study investigates the accumulation of heavy metals cadmium (Cd), copper (Cu), chromium (Cr), and lead (Pb) in the water, sediment, and body parts of whiteleg shrimp (Litopenaeus vannamei) cultured in traditional aquaculture ponds in Sidoarjo, Indonesia. The ICP-AES method showed that heavy metals were more concentrated in sediment than in water, particularly Pb and Cd. Cd levels in water and Pb levels in sediment exceeded national and international quality standards. In shrimp tissue, the highest accumulation occurred in the cephalothorax, especially for Cu, although all detected levels remained within the safety limits set by Indonesian standards. Monitoring heavy metals in the aquaculture environment and shrimp is essential, as these contaminants can bioaccumulate and pose health risks to aquatic organisms and humans throughout the food chain. Water quality parameters were generally suitable for shrimp farming, but low pH values may increase heavy metal solubility and bioaccumulation risks. These findings highlight the need for regular monitoring and better environmental management to ensure the sustainability and safety of traditional shrimp aquaculture systems.
Community Empowerment Strategy of Pondok Agung, Kasembon Sub-district through Biogas Technology to Reduce Cattle Waste Pollution in Water Maimunah, Yunita; Kilawati, Yuni; Muttaqin, Adharul; Amrillah, Attabik Mukhammad; Kartikasari, Dany Primanita
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 8 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i8.9544

Abstract

The community-based biogas project in Kasembon sub-district focuses on utilising livestock manure as a renewable energy source and organic fertiliser producer to improve community welfare and reduce negative environmental impacts. Through an Asset-Based Community Development (ABCD) approach, the project identifies local assets, such as land and manure, and empowers the local community to maintain the biogas system. The biogas production process produces environmentally friendly energy and reduces dependence on LPG. In addition, the organic fertiliser made from biogas waste can be utilised in agriculture to improve productivity and soil health. The implementation of ABCD encourages collaboration between the community, government, and university, and creates new economic opportunities by selling organic fertiliser. However, challenges such as dependence on external assistance, high initial capital requirements, and technical limitations hinder long-term sustainability.
Co-Authors Abdul Rahem Faqih Adharul Muttaqin Adhitya Bhawiyuga Agoes Soeprijanto, Agoes Agum Bayu Gumelar Alif Raditya Amirul Huda Amanda, Talitha Andi Kurniawan Andi Kurniawan Anggita Noer Laily Aprillia Mifthakun Ni’mah Mifthakun Ni’mah Ni’mah Arning W. Ekawati Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Attabik Muhammad Amrillah Attabik Mukhammad Amrillah Ayu Winna Ramadhani Dany Primanita Kartikasari Dany Primanita Kartikasari Dany Primanita Primanita Kartikasari Devi Rahmania Nureka Wasti Dewi Wulandari Diana Aisyah Diana Aisyah Diana Arfiati Dimas Adetia Rikianto Dini Anggraini Endang Yuli Herawati Fahma, Yunita Rifda Alano Fitri Sil Valen Fitri Sil Valen Hafish Gigih Purnama Happy Nursyam Haryono, M. Gandri Herwati Umi Subarijanti Ibdiah, Raudatul Ilham Afandy Afandy Ilham Misbakudin Al Zamzami Islamy, R Adharyan Isroni, Wahyu Iswati Iswati Lini Murni Maftuch Maftuch Maftuch Maftuch Maftuch Maheno Sri Widodo Maheno Sri Widodo, Maheno Sri Marwulan Marwulan Moh. Awaludin Adam MOHAMAD FADJAR Mohamad Fadjar Mohamad Fadjar Mohammad Mahmudi Muhammad Musa Muhammad Nafar Amani Syams Mulyanto, Febri Dwi Novalina Serdiati Nurandi, Nadiah Nurul Mutmainnah, Nurul Pramudia, Zulkisam Punto Apri Sembodo Punto Apri Sembodo Putri, Ardyah R.I. R Adharyan Islamy Raudatul Ibdiah Rizqa, Vylzah Saidah Luthfiyah Sembodo, Punto Apri Sholikah, Lik Anatus Sisca Fajriani Siti Nur Tahirah Sri Andayani Sri Widyarti Sugiarto, Kevin Pratama Suliswanto Suliswanto Susanti, Yogita Ayu Dwi Venny Nur Hidayah Veryl Hasan Win Darmanto Yatris Rambu Tega Yenny Risjani Yuliezar Perwira Dara, Yuliezar Perwira Yunita Maimunah Zahrotur Rosyidah Zamzami, Ilham Misbakudin Al