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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis The Journal of Experimental Life Sciences (JELS) Journal of Food and Life Sciences Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Pembangunan dan Alam Lestari JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Perikanan Universitas Gadjah Mada Samakia: Jurnal Ilmu Perikanan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Journal of Aquaculture and Fish Health Jurnal Lemuru : Jurnal Ilmu Perikanan dan Kelautan Indonesia Jurnal Perikanan Pantura Berkala Penelitian Hayati Fish Scientiae : Jurnal Ilmu-Ilmu Perikanan dan Kelautan Prosiding Seminar Nasional Pengabdian Kepada Masyarakat KOPEMAS Genbinesia Journal of Biology
Yuni Kilawati
Fakultas Perikanan Dan Ilmu Kelautan, Universitas Brawijaya, Malang
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Accumulation of Cd, Cu, Cr, and Pb in Whiteleg Shrimp and Pond Environment of Traditional Aquaculture Systems in Sidoarjo, Indonesia Sugiarto, Kevin Pratama; Kilawati, Yuni; Mahmudi, Mohammad
Jurnal Perikanan Universitas Gadjah Mada Vol 27, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.106352

Abstract

This study investigates the accumulation of heavy metals cadmium (Cd), copper (Cu), chromium (Cr), and lead (Pb) in the water, sediment, and body parts of whiteleg shrimp (Litopenaeus vannamei) cultured in traditional aquaculture ponds in Sidoarjo, Indonesia. The ICP-AES method showed that heavy metals were more concentrated in sediment than in water, particularly Pb and Cd. Cd levels in water and Pb levels in sediment exceeded national and international quality standards. In shrimp tissue, the highest accumulation occurred in the cephalothorax, especially for Cu, although all detected levels remained within the safety limits set by Indonesian standards. Monitoring heavy metals in the aquaculture environment and shrimp is essential, as these contaminants can bioaccumulate and pose health risks to aquatic organisms and humans throughout the food chain. Water quality parameters were generally suitable for shrimp farming, but low pH values may increase heavy metal solubility and bioaccumulation risks. These findings highlight the need for regular monitoring and better environmental management to ensure the sustainability and safety of traditional shrimp aquaculture systems.
Community Empowerment Strategy of Pondok Agung, Kasembon Sub-district through Biogas Technology to Reduce Cattle Waste Pollution in Water Maimunah, Yunita; Kilawati, Yuni; Muttaqin, Adharul; Amrillah, Attabik Mukhammad; Kartikasari, Dany Primanita
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 8 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i8.9544

Abstract

The community-based biogas project in Kasembon sub-district focuses on utilising livestock manure as a renewable energy source and organic fertiliser producer to improve community welfare and reduce negative environmental impacts. Through an Asset-Based Community Development (ABCD) approach, the project identifies local assets, such as land and manure, and empowers the local community to maintain the biogas system. The biogas production process produces environmentally friendly energy and reduces dependence on LPG. In addition, the organic fertiliser made from biogas waste can be utilised in agriculture to improve productivity and soil health. The implementation of ABCD encourages collaboration between the community, government, and university, and creates new economic opportunities by selling organic fertiliser. However, challenges such as dependence on external assistance, high initial capital requirements, and technical limitations hinder long-term sustainability.
Phytochemical profile, antioxidant, and antibacterial activities of Ipomoeae pes-caprae from Pantai Talang Siring Pamekasan, Madura: A promising source of natural bioactive compounds Islamy, R Adharyan; Hasan, Veryl; Serdiati, Novalina; Kilawati, Yuni; Valen, Fitri Sil; Mutmainnah, Nurul
Genbinesia Journal of Biology Vol. 3 No. 2 (2024): March 2024
Publisher : Generasi Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55655/genbinesia.v3i2.49

Abstract

This research investigates the phytochemical composition, antioxidant activity, and antibacterial efficacy of Ipomoea pes-caprae extracts collected from Pantai Talang Siring Pamekasan, Madura. Phytochemical analysis revealed the presence of alkaloids, flavonoids, phenolic compounds, terpenoids, tannins, and saponins in the extracts, indicating their rich bioactive profile. Antioxidant activity assessed via the DPPH assay demonstrated concentration-dependent scavenging of free radicals, with methanol and ethanol extracts exhibiting superior activity compared to the aqueous extract. Antibacterial assays against Escherichia coli and Staphylococcus aureus revealed significant inhibitory effects, with methanol and ethanol extracts displaying greater potency than the aqueous extract. These findings underscore the potential of Ipomoea pes-caprae as a source of natural antioxidants and antimicrobial agents, warranting further investigation into its pharmacological applications and therapeutic potential.
PERAN DAN KEBERADAAN ENZIM METALLOTHIONEIN SEBAGAI PENGIKAT LOGAM BERAT Pb PADA PANGKAL DAN UJUNG RUMPUT LAUT GRACILARIA sp. Tega, Yatris Rambu; Herawati, Endang Yuli; Kilawati, Yuni
JURNAL LEMURU Vol 5 No 1 (2023): JURNAL LEMURU: Jurnal Ilmu Perikanan dan Kelautan Indonesia
Publisher : Program Studi Teknologi Hasil Perikanan|Fakultas Pertanian|Universitas PGRI Banyuwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/jl.v5i1.2440

Abstract

Produksi limbah Industri dan juga buangan limbah rumah tangga yang semakin meningkat dan terjadi terus-menerus, menjadikan sungai tempat pembuangan terakhir sehingga dapat mencemari biota yang berada disekitaran sungai tersebut. Masyarakat di Desa Kupang memanfaatkan aliran sungai tersebut sebagai sumber air untuk budidaya rumput laut Gracilaria sp. Salah satu untuk mengetahui cemaran logam berat pada perairan tersebut dengan mengecek keberadaan kadar Metallothionein pada rumput laut, karena sifat Metallothionein adalah protein yang dapat mengikat logam esensial maupun non esensial. Penelitian ini bertujuan untuk mengetahui kadar Metallothionein pada Gracilaria sp. yang terekspresi pada bagian pangkal dan ujung rumput laut Gracilaria sp. Metode yang digunakan adalah metode deskriptif. Analisis pengujian kadar Metallothionein menggunakan Eliza rider. Hasil penelitian ini bahwa kondisi perairan budidaya tambak Gracilaria sp. untuk parameter suhu, pH, DO sesuai dengan kondisi perairan tambak, tetapi pada parameter salinitas tidak sesuai yaitu melebihi SNI 40 ppt, karena penguapan yang tinggi pada saat musim kemarau, sehingga kadar garamnya tinggi. Kualitas perairan sangat berpengaruh dengan keberadaan Enzim Metallothionein pada pangkal dan ujung rumput laut, Kadar enzim Metallothionein tertinggi terdapat pada bagian ujung Gracilaria sp. yaitu berkisar antara 2.026-2.734 mg/mL. Terjadinya penyerapan yang tinggi karena pada bagian ujung terdapat banyak percabangan kecil dan masih muda sehingga proses penyerapannya masih kuat, sedangkan pada bagian pangkal terdiri dari sel-sel yang sudah tua dengan percabangan yang sedikit dan jumlahnya juga sedikit.
Analysis of Water Quality and Phagocytic Activity (Clarias Sp.) in Aquaponic and Non-Aquaponic Cultivation Systems Sembodo, Punto Apri; Hertika, Asus Maizar Suryanto; Kilawati, Yuni
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.4295

Abstract

Catfish (Clarias sp.) is one of the many fishery biota that has animal protein which is much loved by Indonesian people. One way to develop environmentally friendly catfish cultivation is through the implementation of an aquaponic biofilter system. This research aims to analyze physical, chemical, and biological water quality parameters in catfish cultivation with and without aquaponics, to obtain a description of the non-specific immune system which is analyzed from the conditions of phagocytic activity of catfish cultivated with and without aquaponics. The method used is by taking samples ten times per week by taking water quality parameters as well as blood samples of catfish raised in ponds with and without aquaponics. The analysis used is the unpaired T-test. Based on the results obtained, it was concluded that all parameters showed that the aquaponic cultivation media was better compared to those without aquaponics.
Analysis of Protein Profile and Functional Groups in Biofilms in the Hypersaline Environment Zamzami, Ilham Misbakudin Al; Kilawati, Yuni; Ibdiah, Raudatul; Kurniawan, Andi
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4464

Abstract

Halophilic microorganisms include extremophiles that can live and develop in a hypersaline environment. Biofilm, as microecology, consists of a collection of microorganisms attached to a surface, forming an Extracellular Polymeric Substance (EPS) matrix. This study aims to analyze the protein profile and functional groups of biofilms that grow in hypersaline environments. The method used in this study is the SDS PAGE test to diagnose protein profiling and FTIR to analyze biofilm functional groups. The samples used to grow the biofilm came from salt ponds with different NaCl levels, namely 2%, 25%, and 40%. The results showed that each NaCl treatment had different functional groups in the biofilm samples. In the 2% NaCl treatment, there were peaks with solid intensity at the wavenumber 1100 cm-1, indicating the carboxylic acid functional group. In the 25% NaCl treatment, peaks with solid intensity at wavenumber 2250 cm-1 indicate the isocyanate functional group. In the 40% NaCl treatment, there were peaks with solid intensity at wavenumber 860 cm-1, indicating the alkene functional group. The protein analysis for each treatment shows the presence of pyruvate kinase protein in the biofilm with 2% NaCl. The 25% NaCl biofilm sample contained avidin protein, and the 40% NaCl biofilm sample contained tyrosine protein.
Identifikasi dan Analisis Molekuler Hemosianin sebagai Gen Ketahanan Tubuh Udang Vaname (Litopenaeus vannamei) Amanda, Talitha; Kilawati, Yuni; Maftuch
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.7331

Abstract

Production of white shrimp (Litopenaeus vannamei) tends to decrease every year caused by diseases, one of which is White Feces Disease (WFD). Genetically, monitoring has been carried out on the parent characters of SPF (Specific Pathogen Free) shrimp imported from Hawaii and Florida with low genetic variation. If there is a threat such as disease caused by bacteria and the gene that codes for disease control or the body's resistance gene is missing, it allows the individual to become susceptible to disease. Gene that codes for body resistance in white shrimp is hemocyanin. Hemocyanin gene studies are needed as a first step in controlling disease in white shrimp. Method used is a descriptive method. Procedures carried out are sample collection, detection of WFD, molecular analysis of hemocyanin and data analysis. Results showed that healthy shrimp had hemocyanin while shrimp with WFD had very little or no amount of hemocyanin. Hemocyanin gene profile in healthy shrimp was also analyzed and there were 4 nucleotide change made changes to 4 amino acid. Changes in amino acids affect the stability of hemocyanin and cause polymorphism of hemocyanin. Phylogenetic analysis showed dendogram split into two clades between L. vannamei and P. monodon.
Fraction and Antibacterial Activity of Bitter Melons (Momordica charantia) in Koi Fish (Cypinus carpio) Infected with Aeromonas salmonicida Bacteria and its Effect on Gills Histopathology Nadiah Nurandi; Sri Andayani; Yuni Kilawati
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9338

Abstract

Koi fish (Cyprinus carpio) is a type of freshwater ornamental fish with significant economic value, attracting widespread public interest due to its vibrant colors and agile movements. Therefore, this study introduces a novel approach by evaluating the antibacterial activity of bitter melon extract fraction (Momordica charantia) against A. salmonicida infection in koi fish and its impact on gill histopathology. The novelty of this study lies in the exploration of bitter melon extract fraction as a natural, eco-friendly antibacterial alternative to synthetic antibiotics in aquaculture. This research is crucial as it addresses the growing global concern over antibiotic resistance and the urgent need for sustainable, natural solutions in fish health management. Antibacterial tests were conducted to assess the ability of the bitter melon extract to inhibit bacterial growth, while histopathological analysis aimed to observe the specific changes in gill tissues of infected koi fish. The results revealed that the bitter melon extract fraction significantly reduced bacterial count and improved gill tissue integrity. Histopathological improvements were observed, including reduced hyperplasia, lamellar fusion, edema, and necrosis, indicating tissue recovery. This finding highlights the potential of bitter melon extract fraction as a natural antibacterial agent for controlling bacterial infections in fish, offering an alternative to chemical antibiotics. Further research is needed to determine the optimal dosage and to explore its antibacterial mechanism, ensuring its safe and effective application in aquaculture.
Effect of Maintenance at Different Salinity against White Spot Syndrome Virus (WSSV) Infection Level in Post Larvae Litopenaeus vannamei Shrimp Amrillah, Attabik Mukhammad; Widyarti, Sri; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (961.573 KB) | DOI: 10.21776/ub.jels.2015.005.02.01

Abstract

White spot syndrime virus (WSSV) is a highly contagious disease in shrimp culture and causing 100% death within 3-10 days of clinical symptoms. WSSV can infect shrimp in post larvae stage (PL) to a size of 40 g. This study was aimed to determine the effect of different salinity against WSSV infection level in post larvae vannamei shrimp (Litopenaeus vannamei). In this study PL 40 size of vannamei shrimp infected with WSSV in virus concentration 20 μg.mL-1 in three different salinity is 5 ppt, 15 ppt, 25 ppt for 4 hours using the soaking method then maintened for 7 days post-infection and then infection level and water quality observed. Data analysis used simple linear regression and F test with a confidence level 95% (P <0.05). The results indicate changes in behavior between healthy and infected shrimp in three levels (mild, moderate, severe). The highest total mild infection in salinity treatment of 25 ppt by 30%, the highest total medium infection at salinity treatment 25 ppt by 37%, the highest total severe infection at salinity treatment 5 ppt by 57%. Shrimp that have mild level of infection are increasing in concominant higher salinity, while the severe infection level has decreases with increasing salinity. Keywords: Infection Level, Litopenaeus vannamei, Post Larvae, Salinity, WSSV
The Quality of Ornamental Japanese Carp (Cyprinus carpio var. Koi) After Electroporation as a Gene Material Transfer Method Luthfiyah, Saidah; Soeprijanto, Agoes; Kilawati, Yuni
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1018.176 KB) | DOI: 10.21776/ub.jels.2016.006.02.07

Abstract

Spermatozoa are male gametes that have an advantage in acting as gene transfer media (SMGT), because in transferring genetic material, sperm uses relatively natural vectors. This study was conducted to know sperm quality of Koi after short electroporation with different voltages using Gene Pulser. The study was conducted using a complete randomized design (RAL) with three (3) replications and five (5) treatments, namely: 10 V.cm-1, 20 V.cm-1, 30 V.cm-1, 40 V.cm-1, and 1 control. Electroporation was done with square wave, 1 ms duration of electroporation, 4 times of electroporation shocks with 0.1 s duration. The results showed that electroporation with different voltage levels significantly affected the motility and viability of sperm. Based on orthogonal polynomial analysis, it was known that the treatment affected motility and viability of sperm with a linear equation y = -0.9x + 5, 4 R² = 0.920 and r = 0.96 for motility and y = -11.26x + 91.61 R² = 0.823 and r = 0.91 for viability, meaning that the higher the level of electroporation the lower the viability and sperm motility produced. From the results of this study, it can be suggested that the electroporation of Koi sperm as a gene transfer medium should be performed at a voltage of 10 V.cm-1. Keywords: electroporation, Koi, sperm quality, transgenesis.
Co-Authors Abdul Rahem Faqih Adharul Muttaqin Adhitya Bhawiyuga Agoes Soeprijanto, Agoes Aisyah, Diana Amanda, Talitha Andi Kurniawan Anggita Noer Laily Aprillia Mifthakun Ni’mah Mifthakun Ni’mah Ni’mah Arning W. Ekawati Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Attabik Muhammad Amrillah Attabik Mukhammad Amrillah Ayu Winna Ramadhani Dany Primanita Kartikasari Dany Primanita Kartikasari Dany Primanita Primanita Kartikasari Devi Rahmania Nureka Wasti Dewi Wulandari Diana Aisyah Diana Arfiati Dini Anggraini Endang Yuli Herawati Fahma, Yunita Rifda Alano Fitri Sil Valen Gumelar, Agum Bayu Hafish Gigih Purnama Happy Nursyam Haryono, M. Gandri Hidayah, Venny Nur Huda, Alif Raditya Amirul Ibdiah, Raudatul Ilham Afandy Afandy Ilham Misbakudin Al Zamzami Islamy, R Adharyan Isroni, Wahyu Iswati Iswati Luthfiyah, Saidah Maftuch Maftuch Maftuch Maftuch Maftuch Maheno Sri Widodo, Maheno Sri Marwulan Marwulan Moh. Awaludin Adam MOHAMAD FADJAR Mohamad Fadjar Mohamad Fadjar Mohammad Mahmudi Muhammad Musa Mulyanto, Febri Dwi Murni, Lini Nadiah Nurandi Novalina Serdiati Nurul Mutmainnah, Nurul Pramudia, Zulkisam Punto Apri Sembodo Punto Apri Sembodo Putri, Ardyah R.I. R Adharyan Islamy Raudatul Ibdiah Rikianto, Dimas Adetia Rizqa, Vylzah Sembodo, Punto Apri Sholikah, Lik Anatus Sisca Fajriani Siti Nur Tahirah Sri Andayani Sri Widyarti Subarijanti, Herwati Umi Sugiarto, Kevin Pratama Suliswanto, Suliswanto Susanti, Yogita Ayu Dwi Syams, Muhammad Nafar Amani Veryl Hasan Win Darmanto Yatris Rambu Tega Yenny Risjani Yuliezar Perwira Dara, Yuliezar Perwira Yunita Maimunah Zahrotur Rosyidah Zamzami, Ilham Misbakudin Al