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All Journal Alchemy : Journal of Chemistry Natural B EduChemia: Jurnal Kimia dan Pendidikan Biomedical Journal of Indonesia Jurnal Wiyata : Penelitian Sains dan Kesehatan Darmabakti Cendekia: Journal of Community Service and Engagements Medicra (Journal of Medical Laboratory Science/Technology) Journal of Community Service and Empowerment JOURNAL OF COMMUNITY ENGAGEMENT AND EMPOWERMENT Jurnal Sintesis: Penelitian Sains, Terapan dan Analisisnya Jurnal Pengabdian Kolaborasi dan Inovasi IPTEKS Jurnal Sains Natural Jurnal Pengabdian Masyarakat (Jupemas) Journal of Natural Sciences and Learning WISDOM : Jurnal Pengabdian Kepada Masyarakat Wisdom
Mardiana Prasetyani Putri
Institut Ilmu Kesehatan Bhakti Wiyata Kediri
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Journal : Natural B

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Effect of Adding Ions - Metal Ions to Pectinase Activity Isolation from Bacillus Subtilis on Bleaching Paper Mardiana Prasetyani Putri; Anna Roosdiana; Sasangka Prasetyawan; Diah Mardiana
Natural B, Journal of Health and Environmental Sciences Vol 2, No 1 (2013)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.888 KB) | DOI: 10.21776/ub.natural-b.2013.002.01.11

Abstract

Pectinase enzyme used as biocatalyst to overhaul pectat compounds or pectin. Pectinase avtivity was affected by metal ions. The purpose of this study is to determine the effect of adding metal ions on pectinase activity and its inhibition type.. Determination of enzyme activity was based on galacturonic acid produced, which was analyzed by spectrophotometric using DNS reagent. Concentration of metal ions used i.e. Zn2+, Cu2+, Fe3+ were 2-10 mM. The result showed that Vm and KM number without ions were 161.29 µmol/mL minutes and 0.55%(w/v), respectively. While the presence of ions Zn2+, Cu2+, Fe3+ were producing Vmapp 147.06; 156.25 and 153.85µg.ml-1min-1, KMapp of 0.56%; 0.63% and 0.65%, and also KI of 333,33; 41,38; 32,9. The inhibition to metal ions are non-competitif inhibition.
Co-Authors Algafari Bakti Manggara Amalia, Rifka Anna Roosdiana Arshy Prodyanatasari Asih Imulda Hari Purwani Ayu Sakti Rahmania Azizah, Lia Nur Chrismarani, Estiella Jouvanove Diah Mardiana Elma, Nadya Hari Untarto Swandono Jerhi Wahyu Fernanda Krisnita Dwi Jayanti Lailatul Badriyah Leny Putri Mawarni Lukis, Prima Agusti Mely Purnadianti Mely Purnadianty MM.Riyaniarti Estri Wuryandari Muh. Shofi Muh. Shofi Nelson, Andy Deski Novia Agustina Novia Agustina Novia Agustina, Novia PALUPI SUSILOWATI Purna, Mely Purwasih, Yefi Sasangka Prasetyawan Sintianingtias, Tria Syah, Okty Indrian Yunita Herwidiani Setiawati, Yunita Herwidiani