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All Journal HAYATI Journal of Biosciences VALENSI JURNAL KIMIA SAINS DAN APLIKASI Urania Jurnal Ilmiah Daur Bahan Bakar Nuklir Jurnal Riset Kimia Jurnal Kimia dan Kemasan EduChemia: Jurnal Kimia dan Pendidikan Indonesia Chimica Acta Science and Technology Indonesia Al-Kimia Indonesian Journal of Chemistry Jurnal Pendidikan Bahasa dan Sastra Indonesia Metalingua Jurnal Kimia dan Kemasan Jurnal Pijar MIPA Makara Journal of Science Journal for Quality in Public Health Journal for Research in Public Health
Henny Purwaningsih
Chemistry Department, Faculty Of Mathematics And Natural Science, IPB University, Kampus IPB Dramaga, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Nursing Physics Public Health

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Journal : Science and Technology Indonesia

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Isolation and Characterization of Cellulose Nanofibrils (CNF) from Dates by-Product via Citric Acid Hydrolysis Aisy, Laaili Azti Rohaadatul; Kemala, Tetty; Suryanegara, Lisman; Purwaningsih, Henny
Science and Technology Indonesia Vol. 9 No. 4 (2024): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.4.818-827

Abstract

Industrial residues that are not optimally utilized are removed by burning, landfilling, or dumping, which can threaten the environment and health. In fact, part of this agro-industrial waste still has content that has the potential to become raw material for value-added other industries. Dates by-product as residue of the fiber-rich fruit industry have the potential to be a source of nanocellulose. This study aims to obtain nanofibril cellulose (CNF) isolates from dates by-product via citric acid hydrolysis, and investigate the effect of acid concentration on the unknown dates by-product CNF isolate characteristics. Pretreatment such as delignification and bleaching is needed to obtain cellulose isolate with high purity. Furthermore, acid hydrolysis, centrifugation, and sonication are performed to obtain CNF. CNF isolates are characterized by the analysis of particle size distribution, morphology, and crystallinity. Analysis of functional groups and lignocellulose content test confirm that lignin and hemicellulose are degraded during isolation. The particle size distribution measurement shows that the greater the acid concentration, the smaller the CNF size and the better the size uniformity. The morphology of the CNF obtained is net-like fibers. The degree of crystallinity shown decreases with increasing acid concentration. This study revealed that different citric acid concentrations can result in different characteristics of CNF isolates.
Decolorization of Synthetic Dyes by Laccase of Phellinus noxius BRB 11 Immobilized with Halloysite Nanotubes Naser, Inda Hastuti N; Kemala, Tetty; Yanto, Dede Heri Yuli; Purwaningsih, Henny; Nurhayat, Oktan Dwi; Ardiati, Fenny Clara; Bakar, N.H.H Abu; Iqbal, Anwar
Science and Technology Indonesia Vol. 10 No. 2 (2025): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2025.10.2.644-659

Abstract

Halloysite nanotubes (HNTs) have been proposed as promising supports for enzyme immobilization. Developing an effective technique for enzyme immobilization on HNTs is crucial for their practical application. In this study, we present a method for immobilizing laccase enzymes on HNTs using various amino acids as green cross-linkers for eco-friendly decolorization of synthetic dye. The modified HNTs were characterized using FT-IR, FE-SEM, and HR-TEM analyses. Furthermore, the effects of various parameters—including pH (ranging from 3 to 8), temperature (30-70°C), toxicity, and reusability—on the activity of both free and immobilized laccase were investigated. The results indicated that glycine (Gly) provided the most effective support for laccase immobilization on HNTs. The modified HNTs/Gly-Lac achieved an immobilization yield of 98.45% and successfully decolorized 94.60% of Acid Blue 129 (AB129) within 2 h. The successful immobilization of laccase onto functionalized HNTs was confirmed through FT-IR, FE-SEM, and HR-TEM analyses. Morphological observations of HNTs/Gly for laccase immobilization showed a thickened and roughened appearance with a thin, light gray layer on the outer surface. Additionally, the presence of an imine (-C=N-) bond peak and a peak at 1410 cm-1, corresponding to the (-NH) bond, suggested successful covalent immobilization. The toxicity analysis revealed a reduction in the toxicity of laccase after immobilization onto modified HNTs. A reusability assessment conducted over five cycles demonstrated that decolorization efficiency remained above 45%. These findings suggest that HNTs/GlyLac could serve as an effective material for textile dye wastewater treatment.
Co-Authors A. Sitompul A. Sitompul A.A. Ketut Agung Cahyawan W Abdullah Abdullah Afifi, Muhammad Ridho Afrianto Daud Aini, Zahratul Aisy, Laaili Azti Rohaadatul Akhiruddin Maddu Ali Aulia Ghozali Alif, Alfiah Aliya Nur Hasanah Amalyah Febryanti Amalyah Febryanti Anas Miftah Fauzi Ardiati, Fenny Clara Bakar, N.H.H Abu Boybul Boybul Charlena Dede Heri Yuli YANTO Dyah Iswantini Fitriani Indahsari Fretty Yurike Gufron Wahyudi Ida Ayu Putu Sri Widnyani Inda Iliyin Iqbal, Anwar Koesnadi Lee Wah Lim Lisa Aprilia Indriyani Lisa Aprilia Indriyani Maulidina, Ahsani Mohamad Rafi Mohamad Rafi Muhammad Fathurrahman Naser, Inda Hastuti N Noerpitasari, Erlina Nor Pana Yupa Novik Nurhidayat Novik Nurhidayat Novik Nurhidayat Nurhayat, Oktan Dwi Nurul Shofiah Peni Puspitasari, Peni Purwantiningsih Sugita Putera, Zulmy Faqihuddin Raharjo, Rahmawati Rizki Putri Ramadhani, Rizki Putri Roza Linda Roza Linda Sandu Siyoto, Sandu Setyanto T. Setyanto T., Setyanto Sholahudin, M. Fiqri Sulistioso G.S Sulistioso G.S, Sulistioso SUMINAR SETIATI ACHMADI Suryanegara, Lisman Susanti Susanti Tetty Kemala Tina Rosdiana Triesye Utari Tun Tedja Irawadi Utari, Triesye Wahyudi Priyono Suwarso Widajanti Wibowo Widajanti Wibowo Yanlinastuti Yanlinastuti Zainal Alim Mas’ud