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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa ILMU KELAUTAN: Indonesian Journal of Marine Sciences Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter ASPIRATOR Jurnal Perikanan dan Kelautan BIOMA : Jurnal Biologi Makassar Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Medik Veteriner ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Journal of Parasite Science Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis Current Biomedicine Jurnal Agripet
Risa Tiuria
Divisi Parasitologi Dan Entomologi Kesehatan, Departemen Ilmu Penyakit Hewan Dan Kesmavet, Sekolah Kedokteran Hewan Dan Biomedis, Institut Pertanian Bogor, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Earth & Planetary Sciences Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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 1 
Purifikasi Imunoglobulin Yolk Pada Ayam yang Divaksin terhadap Ekskretori/Sekretori Stadium L3 Ascaridia galli Darmawi Darmawi; Ummu Balqis; Risa Tiuria; Muhammad Hambal; Samadi Samadi
Jurnal Agripet Vol 10, No 2 (2010): Volume 10, No. 2, Oktober 2010
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v10i2.638

Abstract

Purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli L3 larvae stageABSTRACT. The main immunoglobulin fraction of poultry is called IgY, in order to distinguish it from the mammalian IgG. This article focus on purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli larvae to obtained purity IgY. Active vaccinations with excretory/secretory antigen were applied intra muscularly of chickens with an initial dose of 80 g. The vaccinations were repeated three times with dose of each 60 g with an interval of one week. The first vaccinations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by agar gel precipitation test (AGPT). The chickens eggs were collected from 49 day after vaccinations. IgY was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ekscretory/secretory IgY protein was determined by Bradford method ( = 280 nm). The result showed that antibody in yolk was begun detect with AGPT at four weeks after vaccination. IgY concentration after purification was 0,875 0.251 mg/ml. This study has shown that the product released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing antibody through yolk as biologic manufacturer could be a good choice.
Kajian Titer Antibodi Pada Yolk dari Ayam yang Diimunisasi Dengan Antigen Ekskretori/Sekretori Stadium L3 Ascaridia galli Darmawi Darmawi; Ummu Balqis; Risa Tiuria; Muhammad Hambal; Samadi Samadi
Jurnal Agripet Vol 8, No 2 (2008): Volume 8, No. 2, Oktober 2008
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v8i2.611

Abstract

Evaluation of antibody titre in yolk from immunized chickens with excretory/secretory antigen of L3 stage of Ascaridia galliABSTRACT. The purpose of the present study was to trigger humoral immunity of chickens egg yolk exposed to excretory/secretory released in vitro by L3 stage of Ascaridia galli. Amount of 6 head chickens were divided into two groups. First group, the chickens were not immunized. Second group, the chickens were immunized with excretory/ secretory. Active immunizations with excretory/ secretory antigen were applied intra muscularly of chickens with an initial dose of 80 g. The immunizations were repeated three times with dose of each 60 g with an interval of one week. The first immunizations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by enzyme linked immunosorbant assay (ELISA). The result showed that antibody in yolk was begun detect with ELISA increased at two weeks after immunization, This study has shown that the excretory/secretory released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing IgY in yolk.
Populasi Ascaridia galli Dalam Usus Halus Ayam Yang Diberikan Kombinasi Ekskretori/Sekretori L3 dan Imunoglobulin Yolk Darmawi Darmawi; Ummu Balqis; Risa Tiuria
Jurnal Agripet Vol 11, No 2 (2011): Volume 11, No. 2, Oktober 2011
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v11i2.370

Abstract

Ascaridia galli populations in intestine of chickens treated with combination of excretory/secretory L3 and immunoglobulin yolkABSTRACT. The purpose of the present study was to determine the presence of worm populations in intestine of chickens vaccinated and combined with egg yolk to experimental Ascaridia galli infection. Amount of 18 head chickens were devided into six groups (A F). Group A, the chickens were not vaccinated. Group B, the chickens were vaccinated with excretory/secretory of A. galli L3. Group C, the chickens were vaccinated with excretory/secretory of A. galli L3, challenged with dose 1000 L2, and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group D, the chickens were vaccinated with excretory/secretory of A. galli L3 and challenged with dose 1000 L2. Group E, the chickens were challenged with dose 1000 L2 and treated ten times with 0,875 mg egg yolk with an interval of one day intra orally. Group E, the chickens were challenged with dose 1000 L2. Intestinal worm burdens of infected groups were recorded. The result showed that excretory/secretory of A. galli L3 combined with egg yolk decreased significantly A. galli survival in intestine of laying hens. Vaccinations were positively correlated with worm burden at 12 weeks after chalanged. The results suggest that A. galli L3 excretory/secretory product contain potential antigen and that antibody-mediated mechanisms contribute to immune protection.
Co-Authors . Darmawi . Darmawi Adhi Rahmat Hariyadi Adi Winarto Afiff , Usamah Agus Setiyono Akbari, Rizal Arifin Alif Nur Muhamad Muriana April Hari Wardhana Arif Rahman Jabal Arini Ratnasari Aurilia Hemas Adytia Wardaningrum Aviola, Lala Bambang P. Priosoeryanto Bambang Pontjo Priosoeryanto Chairun Nisa Cut Aldila Febiana Damiana Rita Ekastuti Darmawi D Darmawi D Deri Kermelita Dewantoro, Galuh Indro Didit Triatmojo Dita Pratama Putri Dyah Haryuningtyas Savitri Elok Budi Retnani Elsharkawy, Sara Fachriyan H Pasaribu Fachriyan H Pasaribu Fachriyan H. Pasaribu Fachriyan H. Pasaribu Fachriyan Hasymi Pasaribu Fachriyan Hasymi Pasaribu Feri Irawan Fikram, Muhammad Fitri Dinar Rahayu Forcep Rio Indaryanto Forcep Rio Indaryanto Forcep Rio Indaryanto Hadri Latif Hardiyanti, Niken HERA MAHESHWARI hubullah fuadzy Huda Salahudin Darusman Inna Rakhmawati Ismah Atika Salmah Jerica, Monica Silva Jimmy Pangihutan Kusuma, Nurlita Indah M. Jamaluddin Assidiqi M. Jamaluddin Assidiqi, M. Jamaluddin M. Mirsageri M. Mirsageri, M. Maria Natasya Mayasari, Ni Luh Putu Ika Merlia Andriyani Mila Karmila Milda Lestari Mohammad Mukhlis Kamal Muhammad Hambal Muhammad Raihan Pambudi Noviara, Sherly Nugraha, Arifin Budiman Nugraha, Budiman Nurlisa Alias Butet Nursa Rima Putri Putri Indah Ningtias Razali Daud Razali Daud Retno D Soejoedono Retno D Soejoedono Retno D. Soejoedono Retno D. Soejoedono Retno Damayanti Soejoedono Ridi Arif Ridwan Affandi Ripta Mustafa Nugraha Riyan Wahyu Setiadi Rizal Akbari Rizal Arifin Akbari Rizal Arifin Akbari Rizal Arifin Akbari Rizal Arifin Akbari Rizky Diyu Purnama Samadi Samadi Siagian, Tetty Barunawati Simon He Sonja Kleinertz Subangkit, Mawar Sulistiono Suryaningtyas Kusumadewi Susi Soviana Tri Isyani Tungga Dewi Umi Cahyaningsih Ummu Balqis Unita Pratiwi Upik Kesumawati Hadi Vici E.H.S. Susilowati Yusli Wardiatno Yusuf Ridwan Yusuf, Muhammad Baharudin ZK Abdurahman Baizal Zulfikri Mustakdir Zulfiqri Z Zulfiqri Z, Zulfiqri