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All Journal Althea Medical Journal Indonesian Journal of Urology Berkala Ilmu Kesehatan Kulit dan Kelamin Medical Laboratory Technology Journal Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Syntax Literate: Jurnal Ilmiah Indonesia Care : Jurnal Ilmiah Ilmu Kesehatan Oceana Biomedicina Journal Biomolecular and Health Science Journal Medical Laboratory Analysis and Sciences Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Prosiding Seminar Nasional Multidisiplin Journal Orthopaedi and Traumatology Surabaya (JOINTS) Majalah Biomorfologi (Biomorphology Journal) JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL Indian Journal of Forensic Medicine & Toxicology Jurnal Widya Medika Folia Medica Indonesiana

Agung Dwi Wahyu Widodo

Department Of Clinical Microbiology, Faculty Of Medicine Universitas Airlangga, Surabaya, Indonesia – Dr. Soetomo General Hospital Surabaya, Indonesia

Author-ID : 471489

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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The detection of Mycobacterium tuberculosis using in vitro granuloma tuberculosis model intracelluler specimens Imam Agus Faizal; Jusak Nugraha; Agung Dwi Wahyu Widodo
Medical Laboratory Analysis and Sciences Journal Vol 2 No 1 (2020): May 2020
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v2i1.46

The Granulomas are a group of innate and adaptive immune cells that are highly organized, and during tuberculosis infection caused by the bacterium Mycobacterium tuberculosis. Estimated as many as 1.6 million cases of death in 2017 according to World Health Organization. Tuberculosis ranks in the top 10 causes of death worldwide and it is predicted that more than 1.7 billion people (around 25 percent of the world's population) are estimated to be infected by Mycobacterium tuberculosis. The purpose of this study was to confirm the Mtb by examining a new method by modifying Ziehl-Neelsen stain using an intracellular specimens for tuberculous granuloma in vitro. This study used the isolate of the bacterium Mycobacterium tuberculosis H37Rv obtained from the Microbiology Laboratory of the Institute of Tropical Disease Universitas Airlangga, Surabayaa, using Peripheral Blood Mononuclear Cells to make model granuloma tuberculosis. The result is Ziehl-Neelsen staining was modified, it was found that the Mycobacterium tuberculosis H37Rv as detection of bacterial confirmation is said to be successful.

Sepsis: Antibiotic Resistances of Gram-Positive and Gram-Negative Bacterial in a Tertiary Care Hospital Siti Nurul Jannah; Muhammad Vitanata Arfijanto; Musofa Rusli; Agung Dwi Wahyu Widodo
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 12 No. 1 (2021): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V12I12021.29-37

Introduction: Sepsis is a systemic infection that causes multiorgan failure and death. The death rate that is caused by sepsis is increasing. This high value of death has a correlation with the resistance of antibiotics. However, increased antibiotic resistance is not balanced with new research about antibiotics. As a consequence, it causes difficulties in handling sepsis patients who need antibiotic 1-2 hours after diagnosis is enforced. Methods: This was a descriptive study with case study design to analyze medical records of the patients, evaluating the pattern of bacterial resistance to antibiotic in 221 patients with sepsis with 240 blood isolates to identify. Results: From 221 patients identified as sepsis, there were 97 male patients (43.9%) and 124 female patients (56.1%), mostly between 18-59 years old (63.8%), with the highest level in female (54.8 %) and elderly (66.3%). The bacteria that caused the most sepsis were gram-positive. The most species in gram-positive are Staphylococcus haemolyticus (16.3%) and Staphylococcus aureus (12.5%), and the most species in gram-negative is Escheriichia coli (13.3%). The most sensitive antibiotics in gram-positive bacteria were Piperacillin-Tazobactam (100%), Daptomycin (99.2%), and Clindamycin (99.2%). The sensitive antibiotics in gram-negative bacteria were Amikacin (85.9%), Cefoperazone (84.6%), and Piperacillin-Tazobactam (84.1%). Conclusion: The most sensitive antibiotics in gram-positive bacteria were Piperacillin-Tazobactam, Daptomycin, and Clindamycin. The sensitive antibitics in gram-negative bacteria were Amikacin, Cefoperazone, and Piperacillin-Tazobactam.

EVALUASI JARINGAN DALAM STUDI IN VIVO PEMBERIAN EKSTRAK PROPOLIS PADA MODEL LUKA INFEKSI Winona May Hendrata; Willy Sandhika; Agung Dwi Wahyu Widodo
Majalah Biomorfologi Vol. 29 No. 2 (2019): Majalah Biomorfologi
Publisher : Universitas Airlangga, Surabaya, Indonesia

Background: Infection in wound could lead to various consequences including scarring and occurrence of sepsis. With increasing prevalence of resistant strain bacteria, option for treating infected wound need to be constantly expanded. Propolis, a product of honey bee, is a potential treatment which has been studied to have various effects including antibacterial, anti-inflammatory, and antioxidant. Objective: The study aims to assess the effect of propolis toward wound healing, by evaluating wound macroscopic and microscopic appearance. Material and method: Propolis extract were made using maceration technique using ethanol. Thirty Rattus norvegicus male rats were divided into six groups: three groups for treatment and three groups for control. Incision wound were made in the back and was infected with Methicillin ResistantStaphylococcus aureus (MRSA). Propolis was administered orally on daily basis. Wound excision for observation were done on day3, 5, and 7. The tissue were processed using haematoxylin-eosin (HE) staining for microscopic observation. The distance of normal tissue surrounding the wound were measured using image analysis program. Result: Macroscopic observation showed fewer visible wound in the propolis extract treated group. Measurement by microscopic observation on day 3 also demonstrated more narrow wound width in propolis extract treated group (405.75  110.11 μ) compared to the control group (806.20 644.41μ). Conclusion: The result of this study suggests that propolis extract by oral administration promotes the healing of infected wound as shown within microscopic and microscopic observation.

BIOFILM FORMATION AND ANTIMICROBIAL RESISTANCE OF ESCHERICHIA COLI IN VITRO TOWARDS CEFTRIAXONE AND CEFIXIME Jihan Kalishah; Dominicus Husada; M Vitanata Arfijanto; Agung Dwi Wahyu Widodo
Current Internal Medicine Research and Practice Surabaya Journal Vol. 3 No. 1 (2022): CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cimrj.v3i1.32549

Introduction: Escherichia coli is a Gram-negative bacteria and a most cause of nosocomial infections. Bacteria has the ability to form biofilm that may cause antimicrobe resistance. This biofilm protects the microbes from environmental conditions which make it the most cause of nosocomial infections including urinary tract infection and the infections resulted from the use of medical instruments. The aim of this experiment is to observe the presence of cefotaxime and ceftriaxone antimicrobial resistance in E. coli as a cause of biofilm formation. Method: This study conducted three experiments of E. coli sensitivity test on cefotaxime and ceftriaxone, E. coli resistance test after a 48 hours incubation, and optical density measurement using iMark™ ELISA Microplate Reader. Result: The results of sensitivity experiment with antibiotic cefotaxime and ceftriaxone disk showed sensitive result in wild-type E. coli and E. coli ATCC while after 48 hours incubation E. coli ATCC remains sensitive and in wild-type E. coli result are three intermediates, one sensitive and other than that it’s all change to resistance. The third experiment results were obtained from ELISA then classified into three categories. In E. coli ATCC 1 and 2, the results are mostly in high category (OD >0.240). The OD in E. coli 1 is mostly high, while results from E. coli 2 consists of one bacterium in weak category (OD <0.120), four in moderate category (OD 1.20 – 0.240), and three in high category. Conclusion: It can be concluded that E. coli resistance test in cefotaxime and ceftriaxone after 48 hours’ incubation are mostly resistant in wild-type E. coli but sensitive in E. coli ATCC.

RELATIONSHIP BETWEEN FOOD CONTACT TIME TO THE EFFECT ON TRANSFER OF MICROBES FROM CERAMIC FLOOR USING THE FIVE-SECOND RULE Adityawardhana, Taufan; Widodo, Agung Dwi Wahyu; Rehatta, Nancy Margaritta
Journal of Community Medicine and Public Health Research Vol. 2 No. 1 (2021): Journal of Community Medicine and Public Health Research
Publisher : Universitas Airlangga

Eosin-Methylene Blue (EMB) in order to isolate gram-negative bacteria (Escherichia coli) as the contamination level indicator. Microbes identified with gram staining and observed under a light microscope. The result reported into 5 categories: microbes were founded or not in the petri dishes, determine whether lactose fermentation/acid production can be observed, grade the microbes concentration founded in the petri dishes (grade 1-6), classify the level of contamination (low-high), describing colonies shape in EMB agar and identifying the microbes with gram staining. The results was at the student center's canteen 3 of 5 samples under 5 seconds are positive and 5 of 7 samples until 300 seconds are positive. The level of contamination was inconsistent with increasing time. Whereas in the diagnostic center's canteen 12 of 12 samples were all positives, regardless of time. In conclusion, the five-second rule is a significant oversimplification of what actually happens when bacteria transfer from a surface to food. Risk of transfer of contamination is constantly present regardless of time.

Antibiotic Susceptibility of Bacteria Isolated from Open Fracture Grade III Presenting to Dr. Soetomo General Academic Hospital Surabaya Wibisono, Raden Taufan Mulyo; Utomo, Dwikora Novembri; Widodo, Agung Dwi Wahyu
(JOINTS) Journal Orthopaedi and Traumatology Surabaya Vol. 10 No. 1 (2021): April 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joints.v10i1.2021.1-10

Background: Open fractures are a typical case in the orthopedics field. Infection in an open fracture can cause osteomyelitis. An antibiotic susceptibility testing of patient specimen bacteria with open fractures aimed to determine suitable antibiotic agents with which to treat the infectious diseases caused by these bacteria.Methods: This research is a descriptive study to assess antibiotic susceptibility in cases of open fracture grade III in Dr. Soetomo General Academic Hospital, Surabaya. Total sampling was performed on the microbiological culture results of patients diagnosed with open fracture grade III after debridement from October 2018 to September 2019. The identification of the microbes was based on Gram-positive and Gram-negative categories, and the classification was based on susceptibility to antibiotics, whether sensitive, intermediate, and resistant.Results: The examination of the data from the microbiological culture results of patients with a diagnosis of open fracture grade III after debridement from October 2018 to September 2019 in Dr. Soetomo General Hospital Surabaya resulted in 56 research subjects who met the criteria. Acinetobacter baumannii was the most common bacterial species found in the microbiological examination of patients with open fractures, at 15.84%. Cefazoline and ceftriaxone showed low susceptibility. Levofloxacin showed a relatively good value of susceptibility against both Gram-positive and negative bacterial groups.Conclusion: The antibiotic susceptibility pattern of bacteria from specimens in open fracture grade III patients in Dr. Soetomo General Academic Hospital Surabaya varies between each species of bacteria isolate.

Bacterial and Fungal Coinfections in COVID-19 Inpatients at a Tertiary Hospital in Surabaya, Indonesia, from November 2020 to February 2022 Shabira, Naura Ghina; Widodo, Agung Dwi Wahyu; Rusli, Musofa
Current Internal Medicine Research and Practice Surabaya Journal Vol. 6 No. 1 (2025): CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cimrj.v6i1.53331

Introduction: After the first discovery of coronavirus disease 2019 (COVID-19) in 2020, the rapid increase of cases beyond China prompted the declaration of a pandemic. Elevated rates of bacterial-fungal coinfection were observed in hospitalized COVID-19 patients. This study aimed to determine the profiles of bacterial and fungal coinfections in COVID-19 patients diagnosed between November 2020 and February 2022 at Dr. Soetomo General Academic Hospital, Surabaya, Indonesia. Methods: This retrospective descriptive study obtained data from medical records and used total sampling to assemble 278 COVID-19 patients who met the inclusion criteria. The evaluated variables were subject characteristics, including age, sex, comorbidities, length of stay, ventilator use, bacterial coinfections, fungal coinfections, and bacterial-fungal coinfections. Results: The age group of 55–64 years (26.9%) was predominant among the patients, with males (55.4%) outnumbering females. Hypertension was the most prevalent comorbidity (15.8%). The patients were mostly hospitalized for over ten days (66.2%). Eleven patients (4%) were admitted to the intensive care unit, and five patients (1.8%) required mechanical ventilation. The bacterial coinfections were primarily caused by Streptococcus viridans (56.6%), found in 145 sputum specimens, followed by Klebsiella pneumoniae (23.4%). The predominant isolates in the bacterial-fungal coinfections were Streptococcus viridans and Candida albicans (24.8%), identified in 125 sputum specimens, followed by Klebsiella pneumoniae and Candida albicans (18.4%). Conclusion: COVID-19 patients with bacterial-fungal coinfections present certain characteristics, including being male, middle-aged, hypertensive, and hospitalized beyond ten days. Streptococcus viridans, Klebsiella pneumoniae, and Candida albicans are common etiologies in bacterial-fungal coinfections. Highlights: 1. This study overviews the distribution of patients and causative pathogens associated with bacterial, fungal, and bacterial-fungal coinfections, which have escalated with the rapid rise of coronavirus disease 2019 (COVID-19) hospitalization. 2. Data on the causative pathogens of bacterial-fungal coinfection in COVID-19 patients can be considered in the development of guidelines for empirical therapy in clinical practice.

Perbandingan Distribusi Dan Pola Kepekaan Acinetobacter baumannii Terhadap Antimikroba Di RSUD Dr. Soetomo Surabaya Periode Januari–Maret 2015, April–Juni 2015, dan Januari–Maret 2016 Silvia Sutandhio; Agung Dwi Wahyu Widodo; Lindawati Alimsardjono; Eddy Bagus Wasito
JURNAL WIDYA MEDIKA Vol. 4 No. 1 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v4i1.1782

Acinetobacter baumannii is an environmental bacteria that can cause opportunistic infections such as ventilator-associated pneumonia, burn wound infection, urinary tract infection, and sepsis in immunocompromised patients. A. baumannii infections are troublesome because the bacteria resistance to many antimicrobial agents. Surveillance of distribution and sensitivity pattern of A. baumannii is best to be done frequently in hospitals.Venous blood as much as 10 milliliters was drawn aseptically and transferred into liquid medium Bactec® to be sent to Clinical Microbiology Laboratory of Dr. Soetomo General and Teaching Hospital. Medium that showed bacterial growth was subcultured on Blood Agar Plate and MacConkey Agar, and incubated for 18-24 hours in aerobic condition. Identification and susceptibility test were done with semi-automated system BD Phoenix, and interpreted based onClinical and Laboratory Standards Institute 2015.A. baumannii collected on trimester January-March 2015 were 30 isolates, with 19 isolates being Multidrug Resistant(MDR) A. baumannii (63%) and 6 isolatesPandrug Resistant(PDR)A. baumannii (20%). Isolates from trimester April-June 2015 were 32 isolates, with 21 isolates being MDR A. baumannii (66%) and 2 isolates PDR A. baumannii (6%). Isolates from trimester January-March 2016 were 40 isolates, with 22 isolates being MDR A. baumannii (55%) and 2 isolates PDR A. baumannii (5%).A. baumannii mostly isolated from Intensive Care Unit, followed by Internal medicine ward, Pediatric ward, Surgery ward and Emergency Department. High sensitivity of A. baumanniion trimester January-March 2016 was to imipenem (50%), meropenem (50%), amikacin (53%), cotrimoxazole (53%), dan cefoperazone-sulbactam (55%).. MDRA. baumannii mostly isolated from ICU, which reflects high burden of antimicrobial use. At this moment, antimicrobial agents that can be used as empirical therapy for A. baumannii infection are imipenem, meropenem, amikacin, cotrimoxazole, and cefoperazone-sulbactam. The entire hospital member must implement the Infection Control and Prevention and Antimicrobial Stewardship Program to preventnosocomial infections byA. baumanniiand the emergence of MDRA. baumannii.

Distribusi dan Antibiogram MRSA dari Spesimen Darah selama Empat Semester di RSUD Dr. Soetomo Surabaya Silvia Sutandhio; Agung Dwi Wahyu Widodo; Eddy Bagus Wasito
JURNAL WIDYA MEDIKA Vol. 4 No. 2 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v4i2.1812

MRSA bloodstream infection is hospital-acquired problem that is difficult to treat. Bacteria spread to body organs, create multiple loci of infection, and survive in the presence of beta-lactam antibiotics. Study of distribution and antibiogram of MRSA from blood specimens was done in four semesters; i.e. July–December 2014, January–June 2015, July–December 2015, and January–June 2016. Blood of patients suspected for bloodstream infection was drawn aseptically, transferred into liquid medium, and sent to Clinical Microbiology Laboratory of Dr. Soetomo General Hospital. Medium with microbial growth was subcultured on solid medium, and incubated for 18-24 hours in aerobic condition. Identification and susceptibility test were done with BD Phoenix, and interpreted based on Clinical and Laboratory Standards Institute 2015. Of total 107 MRSA isolates; 23 isolates (22%) were collected on July–December 2014, 12 isolates (11%) on January–June 2015, 25 isolates (23%) on July–December 2015, and 47 isolates (44%) on January–June 2016. MRSA mostly isolated from internal disease wards (45%). Isolates are sensitive to linezolid (91%), quinupristin-dalfopristin (83%), fosfomycin (82%), and vancomycin (80%). Trend of MRSA bloodstream infection is increasing. Although antibacterial agents against MRSA are available, it is best to prevent MRSA transmissions and infections.

Analysis of Risk Factors for Antimicrobial Resistance in Bacterial Infections among Diabetic Foot Ulcer Patients William, Andrew; Sembiring, Yan Efrata; Widodo, Agung Dwi Wahyu; Novida, Hermina
Current Internal Medicine Research and Practice Surabaya Journal Vol. 6 No. 2 (2025): CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cimrj.v6i2.64804

Introduction: Diabetic foot ulcer (DFU) is a chronic and progressive complication of diabetes mellitus resulting from macroangiopathy and microangiopathy disorders. Acknowledging the relationship between the Wagner diabetic foot ulcer classification system and infection severity may offer a promising instrument for guiding empirical antibiotic selections in clinical settings. This study aimed to assess the relationship between Wagner grades and the pathogen profiles of patients with DFU, along with their susceptibility to antibiotic therapy.Methods: A cross-sectional study was conducted from January 2021 to August 2023, utilizing 33 secondary datasets obtained from electronic medical records. The data contained the patients' Wagner grades alongside the results of their complete microbiological analysis and antibiotic susceptibility test. The association between determinant factors and patients' pathogen profiles and antibiotic susceptibility patterns was examined using the Chi-square bivariate analysis (p<0.05).Results: Positive culture results were observed in 32 patients (97%), with 59% exhibiting resistance to first-line antibiotics. The most commonly isolated pathogen was Staphylococcus aureus. The antibiotic susceptibility patterns indicated that gentamicin-syn demonstrated the highest activity against Gram-positive bacteria (GPB) isolates, while erythromycin was the most effective against Gram-negative bacteria (GNB) isolates. With escalating Wagner grades, there was an increased proportion of mixed infections, GNB infections (n=8, X²=23.28, p=0.003), and antibiotic resistance (n=8, X²=39.97, p=0.000). GNB isolates showed higher resistance compared to GPB isolates (n=18, X²=42.15, p=0.001).Conclusion: Our findings suggest that DFU patients with varying Wagner grades exhibit different bacterial profiles, infection patterns, and antibiotic sensitivities. Highlights:1. This is the first study conducted in Indonesia to analyze the relationship between the Wagner diabetic foot ulcer classification system and patients' pathogen profiles and antimicrobial susceptibility.2. This study incorporated an in-depth analysis of several infection patterns and the occurrence of antimicrobial resistance, hence offering valuable information on the application of the Wagner classification system not only as a tool for grading infection severity but also for guiding clinicians in selecting the appropriate antibiotics for patients with diabetic foot ulcers.

Co-Authors A.A. Ketut Agung Cahyawan W Adil Jihad Muhammad Adityawardhana, Taufan Ahmad Veri Erianto Alimsardjono, Lindawati Annisa Aulia Maghfirani Sudarmadi Ashari, Muhammad Jundi Aziz Asmarawati, Tri Pudy BaiqIsti Hijriani Boerhan Hidayat Bramantono Bramantono, Bramantono Budiarti, Anita Deby Kusumaningrum Doddy M Soebadi Dominicus Husada Dwikora Novembri Utomo Eddy Bagus Wasito Erianto, Ahmad Veri Fiqih Faizara Ustadi Firmanda, Hafidz Florentina Sustini Hardiono Hardiono, Hardiono heri budiono Hermina Novida, Hermina Hidajat, Boerhan Indiastuti, Danti Nur Jihan Kalishah Jonathan Christianto Sutadji Jusak Nugraha Khairunnida Rahma Larantika Hidayati Lenny Octavia ManikRetno Wahyunitisari Muhammad Vitanata Arfijanto, Muhammad Vitanata Musofa Rusli Nabila, Nabila Nanda Amalia Ramadhanti Nurhidayah, Meiwinda Rizky Pudji Lestari Rachman, Brian Eka Rahma, Khairunnida Rebekah Setiabudi, Rebekah Rehatta, Nancy Margaritta Ronald Pratama Adiwinoto Rusli, Musofa savitri, Lisa Sembiring, Yan Efrata Shabira, Naura Ghina Silvia Sutandhio Siti Nurul Jannah Soelistijo, Soebagijo Adi Subur Prajitno Sunaryo Hardjowijoto Sutadji, Jonathan Christianto Tarmono Djojodimedjo Ulfa Kholili Usman Hadi Wahyunitisari, Manik Retno Warella, Juen Carla Wibisono, Raden Taufan Mulyo William, Andrew Willy Sandhika Winona May Hendrata Yuani Setiawati

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