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All Journal Althea Medical Journal Indonesian Journal of Urology Berkala Ilmu Kesehatan Kulit dan Kelamin Folia Medica Indonesiana Medical Laboratory Technology Journal Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Syntax Literate: Jurnal Ilmiah Indonesia Care : Jurnal Ilmiah Ilmu Kesehatan Oceana Biomedicina Journal Biomolecular and Health Science Journal Medical Laboratory Analysis and Sciences Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Prosiding Seminar Nasional Multidisiplin Journal Orthopaedi and Traumatology Surabaya (JOINTS) Majalah Biomorfologi (Biomorphology Journal) JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL Indian Journal of Forensic Medicine & Toxicology Jurnal Widya Medika

Agung Dwi Wahyu Widodo

Department Of Clinical Microbiology, Faculty Of Medicine Universitas Airlangga, Surabaya, Indonesia – Dr. Soetomo General Hospital Surabaya, Indonesia

Author-ID : 471489

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

Published : 25 Documents Claim Missing Document

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Antibiotic Resistance in Escherichia coli and Staphylococcus aureus from Retail Chicken Meat in Surabaya, Indonesia Annisa Aulia Maghfirani Sudarmadi; Subur Prajitno; Agung Dwi Wahyu Widodo
Biomolecular and Health Science Journal Vol. 3 No. 2 (2020): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v3i2.22170

Introduction: Antimicrobial resistance is becoming a problem in public health. Zoonotic food-borne bacteria is infectious agent that can be transferred from animal to human through the food-producing animal we consume. Nowadays, antibiotic used for human and animal is not only to cure infection but also to aim animal’s growth promotion. It is known as non therapeutic antimicrobial agent (NTA) leading to antibiotic resistance. The third generation cephalosporins, cefotaxime, and also cefoxitin are included as important antibiotic for human. This study aims to identify the presence of cefotaxime-resistant Escherichia coli and cefoxitin-resistant Staphylococcus aureus isolated from chicken meat of both traditional and modern market in Surabaya.Methods: This is descriptive post test only experimental research. We used 8 samples of chicken meat from 4 different market using purposive sampling technique. We cultured Escherichia coli and Staphylococcus aureus from the chicken meat. Sensitivity test was done using Kirby-bauer disk-diffusion method.Results: All chicken meat sample bought from traditional market in Surabaya are contaminated by cefotaxime-sensitive Escherichia coli (n=4/4) while chicken meat sample bought from modern market are not contaminated by Escherichia coli (n=0/4). All chicken meat sample bought from traditional (n=4/4) are also contaminated by cefoxitin-sensitive Staphylococcus aureus. Half of chicken meat sample bought from modern market (n=2/4) are contaminated by cefoxitin-sensitive Staphylococcus aureus, while the other half (n=2/4) are contaminated by cefoxitin-resistant Staphylococcus aureus.Conclusion: Antibiotic resistance is found and all chicken meat samples have been highly contaminated with bacteria therefore food-processing should be done correctly.

The detection of Mycobacterium tuberculosis using in vitro granuloma tuberculosis model intracelluler specimens Imam Agus Faizal; Jusak Nugraha; Agung Dwi Wahyu Widodo
Medical Laboratory Analysis and Sciences Journal Vol 2 No 1 (2020): May 2020
Publisher : Department of D3 Medical Technology Laboratory STIKes Hutama Abdi Husada Tulungagung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35584/melysa.v2i1.46

The Granulomas are a group of innate and adaptive immune cells that are highly organized, and during tuberculosis infection caused by the bacterium Mycobacterium tuberculosis. Estimated as many as 1.6 million cases of death in 2017 according to World Health Organization. Tuberculosis ranks in the top 10 causes of death worldwide and it is predicted that more than 1.7 billion people (around 25 percent of the world's population) are estimated to be infected by Mycobacterium tuberculosis. The purpose of this study was to confirm the Mtb by examining a new method by modifying Ziehl-Neelsen stain using an intracellular specimens for tuberculous granuloma in vitro. This study used the isolate of the bacterium Mycobacterium tuberculosis H37Rv obtained from the Microbiology Laboratory of the Institute of Tropical Disease Universitas Airlangga, Surabayaa, using Peripheral Blood Mononuclear Cells to make model granuloma tuberculosis. The result is Ziehl-Neelsen staining was modified, it was found that the Mycobacterium tuberculosis H37Rv as detection of bacterial confirmation is said to be successful.

Sepsis: Antibiotic Resistances of Gram-Positive and Gram-Negative Bacterial in a Tertiary Care Hospital Siti Nurul Jannah; Muhammad Vitanata Arfijanto; Musofa Rusli; Agung Dwi Wahyu Widodo
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 12 No. 1 (2021): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V12I12021.29-37

Introduction: Sepsis is a systemic infection that causes multiorgan failure and death. The death rate that is caused by sepsis is increasing. This high value of death has a correlation with the resistance of antibiotics. However, increased antibiotic resistance is not balanced with new research about antibiotics. As a consequence, it causes difficulties in handling sepsis patients who need antibiotic 1-2 hours after diagnosis is enforced. Methods: This was a descriptive study with case study design to analyze medical records of the patients, evaluating the pattern of bacterial resistance to antibiotic in 221 patients with sepsis with 240 blood isolates to identify. Results: From 221 patients identified as sepsis, there were 97 male patients (43.9%) and 124 female patients (56.1%), mostly between 18-59 years old (63.8%), with the highest level in female (54.8 %) and elderly (66.3%). The bacteria that caused the most sepsis were gram-positive. The most species in gram-positive are Staphylococcus haemolyticus (16.3%) and Staphylococcus aureus (12.5%), and the most species in gram-negative is Escheriichia coli (13.3%). The most sensitive antibiotics in gram-positive bacteria were Piperacillin-Tazobactam (100%), Daptomycin (99.2%), and Clindamycin (99.2%). The sensitive antibiotics in gram-negative bacteria were Amikacin (85.9%), Cefoperazone (84.6%), and Piperacillin-Tazobactam (84.1%). Conclusion: The most sensitive antibiotics in gram-positive bacteria were Piperacillin-Tazobactam, Daptomycin, and Clindamycin. The sensitive antibitics in gram-negative bacteria were Amikacin, Cefoperazone, and Piperacillin-Tazobactam.

EVALUASI JARINGAN DALAM STUDI IN VIVO PEMBERIAN EKSTRAK PROPOLIS PADA MODEL LUKA INFEKSI Winona May Hendrata; Willy Sandhika; Agung Dwi Wahyu Widodo
Majalah Biomorfologi Vol. 29 No. 2 (2019): Majalah Biomorfologi
Publisher : Universitas Airlangga, Surabaya, Indonesia

Background: Infection in wound could lead to various consequences including scarring and occurrence of sepsis. With increasing prevalence of resistant strain bacteria, option for treating infected wound need to be constantly expanded. Propolis, a product of honey bee, is a potential treatment which has been studied to have various effects including antibacterial, anti-inflammatory, and antioxidant. Objective: The study aims to assess the effect of propolis toward wound healing, by evaluating wound macroscopic and microscopic appearance. Material and method: Propolis extract were made using maceration technique using ethanol. Thirty Rattus norvegicus male rats were divided into six groups: three groups for treatment and three groups for control. Incision wound were made in the back and was infected with Methicillin ResistantStaphylococcus aureus (MRSA). Propolis was administered orally on daily basis. Wound excision for observation were done on day3, 5, and 7. The tissue were processed using haematoxylin-eosin (HE) staining for microscopic observation. The distance of normal tissue surrounding the wound were measured using image analysis program. Result: Macroscopic observation showed fewer visible wound in the propolis extract treated group. Measurement by microscopic observation on day 3 also demonstrated more narrow wound width in propolis extract treated group (405.75  110.11 μ) compared to the control group (806.20 644.41μ). Conclusion: The result of this study suggests that propolis extract by oral administration promotes the healing of infected wound as shown within microscopic and microscopic observation.

BIOFILM FORMATION AND ANTIMICROBIAL RESISTANCE OF ESCHERICHIA COLI IN VITRO TOWARDS CEFTRIAXONE AND CEFIXIME Jihan Kalishah; Dominicus Husada; M Vitanata Arfijanto; Agung Dwi Wahyu Widodo
Current Internal Medicine Research and Practice Surabaya Journal Vol. 3 No. 1 (2022): CURRENT INTERNAL MEDICINE RESEARCH AND PRACTICE SURABAYA JOURNAL
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cimrj.v3i1.32549

Introduction: Escherichia coli is a Gram-negative bacteria and a most cause of nosocomial infections. Bacteria has the ability to form biofilm that may cause antimicrobe resistance. This biofilm protects the microbes from environmental conditions which make it the most cause of nosocomial infections including urinary tract infection and the infections resulted from the use of medical instruments. The aim of this experiment is to observe the presence of cefotaxime and ceftriaxone antimicrobial resistance in E. coli as a cause of biofilm formation. Method: This study conducted three experiments of E. coli sensitivity test on cefotaxime and ceftriaxone, E. coli resistance test after a 48 hours incubation, and optical density measurement using iMark™ ELISA Microplate Reader. Result: The results of sensitivity experiment with antibiotic cefotaxime and ceftriaxone disk showed sensitive result in wild-type E. coli and E. coli ATCC while after 48 hours incubation E. coli ATCC remains sensitive and in wild-type E. coli result are three intermediates, one sensitive and other than that it’s all change to resistance. The third experiment results were obtained from ELISA then classified into three categories. In E. coli ATCC 1 and 2, the results are mostly in high category (OD >0.240). The OD in E. coli 1 is mostly high, while results from E. coli 2 consists of one bacterium in weak category (OD <0.120), four in moderate category (OD 1.20 – 0.240), and three in high category. Conclusion: It can be concluded that E. coli resistance test in cefotaxime and ceftriaxone after 48 hours’ incubation are mostly resistant in wild-type E. coli but sensitive in E. coli ATCC.

Bacterial Profile and Antibiotic Use in Catheter-Associated Urinary Tract Infection Patients at a Tertiary Referral Hospital in Surabaya, Indonesia Ahmad Veri Erianto; Tri Pudy Asmarawati; Agung Dwi Wahyu Widodo; Brian Eka Rachman
Folia Medica Indonesiana Vol. 59 No. 2 (2023): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v59i2.39919

Highlights: This study examined patients with catheter-associated urinary tract infections (CAUTI) to find the prevalent bacteria and the frequency of antibiotic use. Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli sensitive to amikacin was found to be the most common pathogen in CAUTI, while ceftriaxone was the most commonly used antibiotic. Abstract Catheter-associated urinary tract infection (CAUTI) is the most typical type of infection associated with healthcare. This infection constitutes a significant health problem due to its complications and frequent recurrence. The high annual incidence of CAUTI leads to infectious complications and a high cost of treatment. Antibiotic-resistant bacteria further complicate the problem because multiresistant pathogenic bacteria are often responsible for these infections. Moreover, the lack of a comprehensive case reporting system in Indonesia hinders the determination of CAUTI incidence. This study aimed to determine the bacterial profile and antibiotic use in CAUTI patients (n=22) at the Internal Medicine Inpatient Unit of Dr. Soetomo General Academic Hospital, Surabaya, Indonesia. This retrospective descriptive study collected data from patient medical records to describe the incidence, type of bacteria, sensitivity patterns, and antibiotic use in CAUTI patients. The data collection process included collecting medical records, recording information on data collection sheets, and recapitulating and analyzing the data. The criteria for CAUTI diagnosis were a bacterial count of ≥105 CFU/mL and a catheterization that lasted more than 48 hours. Samples were selected using non-probability sampling, also known as saturation sampling. Meanwhile, variables analyzed in this study were the type of pathogenic bacteria, antibiotics used, age, and sex. Descriptive analysis was employed to analyze the data. The findings suggested that the most common bacteria were Escherichia coli, with eight of nine bacteria showing extended-spectrum beta-lactamase (ESBL) production. Female patients aged 35–65 years were the majority of the CAUTI patients. Amikacin was the antibiotic with the highest sensitivity (90.9%), while ceftriaxone was the most common type of antibiotic administered to the patients. In addition, intravenous (IV) administration was the most common route of antibiotic administration, with an average therapy duration of seven days. In conclusion, identifying the type of pathogenic bacteria and performing antibiotic susceptibility testing can help minimize antibiotic resistance and prevent more severe complications in CAUTI patients.

Antifungal Activity of Selaginella plana (Desv. ex Poir.) Hieron Extract Against Candida albicans In Vitro Juen Carla Warella; Khairunnida Rahma; Agung Dwi Wahyu Widodo; Rebekah Juniati Setiabudi
Folia Medica Indonesiana Vol. 59 No. 3 (2023): September
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/fmi.v59i3.44165

Highlights: 1. A study on the medical benefits of Selaginella plana has significant academic value due to its extensive traditional usage among the Moluccan people as a medicinal remedy, especially for its antifungal properties.2. The findings of this study will allow further screening to determine the mechanism of action of bioactive compounds in inhibiting the growth of Candida albicans Abstract Candidiasis is an opportunistic infection caused by Candida albicans. This infection commonly affects the skin, oral mucosa, vagina, and gastrointestinal tract. Excessive use of azole antifungals in the treatment of Candida albicans infections can lead to the development of resistance. Therefore, it is necessary to explore alternative treatments using medicinal plants such as Selaginella plana, commonly referred to as “rutu-rutu” in a local language spoken across Maluku, Indonesia. Selaginella plana contains active compounds belonging to various chemical classes, including terpenoids, steroids, flavonoids, and saponins. This study aimed to determine the ability of Selaginella plana extract as an antifungal agent against Candida albicans by evaluating its inhibitory and antifungal effects. This study used an actual experimental design and broth dilution method. The research methodology involved the extraction of Selaginella plana using a solvent of 96% ethanol. The extract was then prepared in various concentrations, i.e., 100%, 50%, 25%, 12.5%, 6.25%, and 3.125%. Additionally, ketoconazole and distilled water were included in the experiment for the positive and negative controls, respectively. The results of this study demonstrated that Selaginella plana extract inhibited the growth of Candida albicans when administered starting at a concentration of 12.5%. However, the antifungal potential of Selaginella plana extract that induced cell death was only observed at a concentration of 100%. The fungicidal activity was exclusively identified in the undiluted, pure extract. The inhibitory and cytotoxic effects of Selaginella plana on Candida albicans cells were attributed to the presence of bioactive compounds in Selaginella plana, including flavonoids, tannins, terpenoids, and saponins. These bioactive compounds had the ability to inhibit cell growth by altering membrane permeability, causing mitochondrial dysfunction, and disrupting ergosterol biosynthesis. It can be concluded that Selaginella plana extract can act as a fungistatic agent against the proliferation of Candida albicans.

Pola Kuman Serta Kepekaan Antibiotik pada Pasien Sepsis di Ruang Rawat Inap Penyakit Dalam RSUD dr. Soetomo Surabaya Nabila Nabila; Bramantono Bramantono; Deby Kusumaningrum; Agung Dwi Wahyu Widodo
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v9i8.17294

Sepsis adalah disfungsi organ yang mengancam jiwa yang disebabkan oleh disregulasi respon host terhadap infeksi. Penatalaksanaan sepsis salah satunya adalah pemberian antibiotik spesifik, namun pengobatan awal adalah pemberian antibiotik spektrum luas sebelum ada hasil kultur. Penggunaan antibiotik yang tidak tepat pada pasien septik dapat menyebabkan resistensi bakteri dan memperburuk kondisi pasien. Tujuan penelitian ini adalah mempelajari pola sensitivitas bakteri dan antibiotik pada pasien sepsis di RSUD Dr. Soetomo Surabaya Metode. Penelitian deskriptif retrospektif ini dilakukan di RSUD Dr. Soetomo pada bulan Januari sampai dengan Desember 2019. Sampel penelitian ini adalah seluruh data rekam medis pasien sepsis yang memiliki hasil kultur darah bakteri positif di Laboratorium Mikrobiologi Klinik RSUD Dr. Soetomo Surabaya di Hasil 2019. Hasil penelitian menunjukkan bahwa dari 722 sampel darah, termasuk 124 (17,2%) sampel dengan hasil kultur positif. Pasien termuda berusia 16 tahun dan tertua berusia 97 tahun. Hasil ini juga menunjukkan bahwa sebagian besar pasien sepsis adalah perempuan. Bakteri yang paling banyak ditemukan adalah Staphylococcus spp. 80,4%. Bakteri gram positif (Staphylococcus spp.) sensitif terhadap linezolid, vankomisin, teicoplanin Kesimpulan. Bakteri yang paling banyak ditemukan adalah Staphylococcus spp, sedangkan antibiotik yang paling sensitif terhadap Staphylococcu spp gram positif adalah antibiotik linezolid, vankomisin, dan teicoplanin.

RELATIONSHIP BETWEEN FOOD CONTACT TIME TO THE EFFECT ON TRANSFER OF MICROBES FROM CERAMIC FLOOR USING THE FIVE-SECOND RULE Adityawardhana, Taufan; Widodo, Agung Dwi Wahyu; Rehatta, Nancy Margaritta
Journal of Community Medicine and Public Health Research Vol. 2 No. 1 (2021): Journal of Community Medicine and Public Health Research
Publisher : Universitas Airlangga

Eosin-Methylene Blue (EMB) in order to isolate gram-negative bacteria (Escherichia coli) as the contamination level indicator. Microbes identified with gram staining and observed under a light microscope. The result reported into 5 categories: microbes were founded or not in the petri dishes, determine whether lactose fermentation/acid production can be observed, grade the microbes concentration founded in the petri dishes (grade 1-6), classify the level of contamination (low-high), describing colonies shape in EMB agar and identifying the microbes with gram staining. The results was at the student center's canteen 3 of 5 samples under 5 seconds are positive and 5 of 7 samples until 300 seconds are positive. The level of contamination was inconsistent with increasing time. Whereas in the diagnostic center's canteen 12 of 12 samples were all positives, regardless of time. In conclusion, the five-second rule is a significant oversimplification of what actually happens when bacteria transfer from a surface to food. Risk of transfer of contamination is constantly present regardless of time.

PROFIL RASIO NEUTROFIL TERHADAP LIMFOSIT PADA PASIEN DENGAN DUGAAN DEMAM TIFOID DI RSUD DR. SOETOMO SURABAYA Meiwinda Rizky Nurhidayah; Muhammad Vitanata Arfijanto; Agung Dwi Wahyu Widodo; Ulfa Kholili
Care : Jurnal Ilmiah Ilmu Kesehatan Vol 9, No 1 (2021): EDITION MARCH 2021
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/jc.v9i1.2042

Demam tifoid merupakan penyakit multisistem akut yang disebabkan oleh Salmonella typhi dan bersifat endemis. Rendahnya sensitivitas alat diagnostik menyebabkan under diagnosed maupun over diagnosed. Beberapa studi menggunakan rasio neutrofil limfosit sebagai parameter status inflamasi. Studi ini untuk mengetahui gambaran rasio neutrofil limfosit pada pasien dengan dugaan demam tifoid. Sebuah studi deskriptif observasional dengan desain lintang potong menggunakan data sekunder dari rekam medis di Rumah Sakit Umum Daerah Dr. Soetomo Surabaya tahun 2016-2018. Teknik pengambilan sampel adalah total sampling dan diperoleh 64 sampel. Jumlah pasien dengan jenis kelamin laki-laki sebanyak 53,1% dan perempuan sebanyak 46,9% Perbandingan pasien laki-laki dan perempuan 1,3:1 dengan rentang usia terbanyak 17-25 tahun. Gambaran klinis rerata lama demam yaitu 6,95 hari dengan rentang lama demam terbanyak 1-7 hari. Demam ditunjukkan pada semua pasien (100%). Pemeriksaan hematologi kadar hemoglobin, leukosit dan trombosit dalam batas normal. Hasil neutrofil dan limfosit dalam batas normal. Gambaran rasio neutrofil limfosit memiliki median 3,97 dengan rentang nilai dalam batas normal.

Co-Authors A.A. Ketut Agung Cahyawan W Adil Jihad Muhammad Adityawardhana, Taufan Ahmad Veri Erianto Alimsardjono, Lindawati Annisa Aulia Maghfirani Sudarmadi Asmarawati, Tri Pudy BaiqIsti Hijriani Boerhan Hidayat Bramantono Bramantono, Bramantono Deby Kusumaningrum Doddy M Soebadi Dominicus Husada Dwikora Novembri Utomo Eddy Bagus Wasito Fiqih Faizara Ustadi Florentina Sustini Hardiono Hardiono, Hardiono heri budiono Hermina Novida, Hermina Hidajat, Boerhan Indiastuti, Danti Nur Jihan Kalishah Jonathan Christianto Sutadji Jusak Nugraha Khairunnida Rahma Larantika Hidayati Lenny Octavia ManikRetno Wahyunitisari Meiwinda Rizky Nurhidayah Muhammad Vitanata Arfijanto Musofa Rusli Nabila Nabila Nanda Amalia Ramadhanti Pudji Lestari Rachman, Brian Eka Rebekah Setiabudi, Rebekah Rehatta, Nancy Margaritta Ronald Pratama Adiwinoto Rusli, Musofa savitri, Lisa Sembiring, Yan Efrata Shabira, Naura Ghina Silvia Sutandhio Siti Nurul Jannah Soelistijo, Soebagijo Adi Subur Prajitno Sunaryo Hardjowijoto Tarmono Djojodimedjo Ulfa Kholili Usman Hadi Warella, Juen Carla Wibisono, Raden Taufan Mulyo William, Andrew Willy Sandhika Winona May Hendrata Yuani Setiawati

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