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All Journal Jurnal Veteriner Jurnal Kedokteran Syiah Kuala Indonesian Journal of Urology Folia Medica Indonesiana Majalah Obstetri dan Ginekologi The Indonesian Biomedical Journal Majalah Kesehatan FKUB Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Respirologi Indonesia Majalah Patologi Indonesia Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Indonesian Journal of Electrical Engineering and Computer Science Jurnal Layanan Masyarakat (Journal of Public Service) Indonesian Applied Physics Letters Jurnal Pengabdian Masyarakat dalam Kesehatan Indian Journal of Forensic Medicine & Toxicology Jurnal Pengabdian Masyarakat Bhinneka Jurnal Pengabdian Masyarakat Bangsa
Anny Setijo Rahaju, Anny Setijo
Departemen Patologi Anatomi, Fakultas Kedokteran, Universitas Airlangga
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THE MUTATION STATUS OF KRAS GENE CODON 12 AND 13 IN COLORECTAL ADENOCARCINOMA (Status Mutasi Gen Kras Kodon 12 dan 13 di Adenocarcinoma Kolorektal) Gondo Mastutik; Alphania Rahniayu; Anny Setijo Rahaju; Nila Kurniasari; Reny I’tishom
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1177

Abstract

Kanker kolorektum merupakan salah satu kanker yang tersering di dunia. Target molekuler untuk pengobatan kanker kolorektumyaitu Epidermal Growth Factor Receptor (EGFR) dengan pemberian antibodi monoklonal anti-EGFR. Pemberian pengobatan ini tidakdapat memberikan efek dampak di pasien dengan status gen KRAS bentuk mutan, sehingga perlu dilakukan pemeriksaan status mutasigen KRAS. Telitian berupa deskriptif dengan pendekatan potong lintang yang bertujuan untuk mendapatkan data status mutasi genKRAS kodon 12 dan 13 di pasien adenocarcinoma colorectal. Deteksi mutasi KRAS dilakukan dengan teknik Polymerase Chain ReactionRestriction Fragment Length Polymorphism (PCR RFLP) yang dikonfirmasi dengan sekuensing. Sampel telitian adalah 30 blok parafinyang diperoleh dari Rumah Sakit Dr.Soetomo Surabaya masa waktu Januari-Desember 2013. Setelah dilakukan ekstraksi DNA terdapat21 sampel yang dapat digunakan untuk pemeriksaan lanjutan. Hasil PCR RFLP menunjukkan terdapat 7/21 mutasi pada kodon12 dan tidak terdapat mutasi gen KRAS pada kodon 13. Mutasi pada kodon 12 yaitu GGT>GCT, GGT>GGA dan GGT>GAT yangmenyebabkan perubahan asam amino Gly12Ala, Gly12Gly dan Gly12Asp. Simpulan telitian ini adalah mutasi gen KRAS kodon 12 padaadenocarcinoma colorectal di Rumah Sakit Dr. Soetomo Surabaya sebanyak 33%.
Correlation Between Pleural Fluid GenXpert® and Histopathology Finding of Pleural Biopsy in Tuberculous Pleural Effusion Sheilla Matheos; Isnin Anang Marhana; Anny Setijo Rahaju
Jurnal Respirologi Indonesia Vol 38, No 1 (2018)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v38i1.133

Abstract

Backgrounds: Tuberculosis pleural effusion is the most common extrapulmonary TB after lymphadenitis TB, but a definite diagnosis is still a challenge. Pleural biopsy has historically been the gold standard procedure for the diagnosis of pleural tuberculosis. GenXpert® is a computerize test based in nucleic acid amplification tahat automatically detect MTB and rifampicin resistance. This study aimed to prove the correlation between pleural fluid GenXpert® and histopathological findings of pleural biopsy in patients with pleural tuberculosis. Methods: This study used an observational analytic design with a cross-sectional design conducted in Dr. Soetomo Hospital, Surabaya in March-June 2017. Statistic analysis was using chi square test and contingensi coofisient. The pleural GenXpert® was tested in 23 patients with pleural effusion and their biopsy speciments underwent histopathological analysis. Results: Histopathological findings of pleural biopsy was positive in 4 subjects and pleural fluid GenXpert® resulted positive in 6 subjects. There was significant correlation between pleural fluid GenXpert® and histopathological findings of specimen pleural biopsy in patients with pleural tuberculosis (P=0.040) with moderate strenght (P=0.014). Sensitivity and specificity of pleural fluid GenXpert® were 75.0% dan 84.2% respectively. Conclusions: There was significant correlation between pleural fluid GenXpert® and histopathological findings of pleural biopsy in patients with pleural tuberculosis. (J Respir Indo 2018; 38(1): 1-6)
Tubule Formation Segmentation Of Histopathological Image Of Breast Cancer By Using Clustering Method Hadiyyatan Waasilah; Riries Rulaningtyas; Winarno Winarno; Anny Setijo Rahaju
Indonesian Applied Physics Letters Vol. 1 No. 1 (2020): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/iapl.v1i1.21338

Abstract

Histopathological assessment is one of the examinations that allows the classification of breast cancer based on its level. Histopathological assessment factors are based on tubule formation, nuclear pleomorphism, and the mitotic count. This study only focused on tubule formation. The tubule formation was represented by a lumen surrounded a  nucleus. The segmentation of tubule histopathology of breast cancer method was using a combination of k-means clustering and graph cut. The image data used in this study were 15 images of breast cancer histopathology preparations using 5 variations in the number of clusters (k) in the k-means clustering method. The best results of tubule formation segmentation using k = 4, with an average value of balanced accuracy was 81.08% and the most optimal balanced accuracy results was 94.34%.
EARLY DETECTION OF BREAST AND CERVICAL CANCER OF THE RESIDENTS OF PROPPO PAMEKASAN DISTRICT Dyah Fauziah; Alphania Rahniayu; Nila Kurniasari; Anny Setijo Rahaju; Etty Hary Kusumastuti; Gondo Mastutik; I Ketut Sudiana
Jurnal Pengabdian Masyarakat Dalam Kesehatan Vol. 3 No. 1 (2021): APRIL 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jpmk.v3i1.21584

Abstract

Introduction: Breast cancer and cervical cancer are common cancers in females. Both of these cancers can be detected early. Early detect vion can reduce cancer morbidity and mortality. The community outreach activities are required to increase knowledge about breast cancer and cervical cancer, in addition to screening as preventive efforts for these two cancers.Methods: The activity of raising knowledge about the prevention and early detection of breast cancer and cervical cancer was carried out through community counseling to 94 females who were members of the PKK and young females in the Kecamatan Proppo, Kabupaten Pamekasan. Pre and post tests were given before and after the community counseling. After the community counseling, 86 participants were screened for breast cancer by clinical palpation and 46 participants were screened for cervical cancer using the pap smear method.Results: The result of the post test showed a 27.53% increase in knowledge. The results of the pap smear examination on participants showed 96% concluded as Papanicolau Class II, 2% Class I, and 2% Class IV. The patient with class IV results was referred to an obstetrician.Conclusion: Community counseling about breast self examination (BSE) and Pap smear can increase people's knowledge about early detection of breast cancer and cervical cancer.
PENYULUHAN TENTANG TANDA AWAL DAN DETEKSI DINI TUMOR GANAS PAYUDARA PADA WARGA DI PUSKESMAS SEMEMI KECAMATAN BENOWO KOTA SURABAYA Nila Kurniasari; Gondo Mastutik; Dyah Fauziah; Etty Hary Kusumastuti; Alphania Rahniayu; Anny Setijo Rahaju
Jurnal Layanan Masyarakat Vol. 6 No. 2 (2022): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v6i2.2022.251-258

Abstract

Kanker payudara merupakan keganasan paling sering dan penyebab kematian tertinggi. Di Indonesia, kanker payudara juga merupakan kanker paling sering dan penyebab kematian kedua setelah kanker paru. Hal ini tejadi karena kanker payudara sering terdiagnosis pada stadium lanjut sehingga menyebabkan kegagalan pengobatan dan kematian. Kejadian kanker payudara stadium lanjut ini dapat dicegah dengan deteksi dini, namun masyarakat masih mempunyai pengetahuan yang rendah tentang tanda awal kanker payudara. Tujuan dari kegiatan ini adalah untuk memberikan penyuluhan tentang tanda awal dan deteksi dini tumor ganas di payudara. Kegiatan dilaksanakan pada 11 November 2020, diikuti oleh 91 orang ibu PKK di wilayah kerja Pusat Kesehatan Masyarakat (Puskesmas) Sememi, Kecamatan Benowo Kota Surabaya. Kegiatan diawali pre-test dan pengisian kuisioner faktor risiko, kemudian dilanjutkan dengan pemberian penyuluhan melalui aplikasi zoom meeting dan diakhiri dengan post-test. Evaluasi dilakukan dengan membandingkan hasil pre-test dan post-test. Hasil kuisioner menunjukkan terdapat peningkatan pemahaman tentang tanda awal dan deteksi dini kanker payudara sebesar 6,96%. Faktor risiko peserta menderita kanker payudara menunjukkan bahwa sebagian besar peserta penyuluhan tidak berisiko menderita kanker payudara karena tidak merokok (100%), tidak mempunyai riwayat benjolan pada payudara (97,14%), menyusui lebih dari 6 bulan (88,57%), tidak terpapar radiasi sinar X (88,57%), mempunyai anak (85,71%), tidak mempunyai riwayat keluarga yang pernah menderita tumor atau kanker (71,43%), rutin berolah raga (60%), namun terdapat 97,14% yang berusia lebih dari 25 tahun sehingga masih mempunyai faktor risiko menderita kanker payudara. Kesimpulan. Terdapat peningkatan pengetahuan masyarakat sebesar 6.96% dan sebagain besar peserta penyuluhan tidak memiliki faktor risiko untuk terjadi kanker payudara.
IMPROVING KNOWLEDGE ABOUT EARLY DETECTION AND SCREENING OF BREAST CANCER AMONG FEMALE RESIDENTS IN GANDUL VILLAGE, PILANGKENCENG DISTRICT, MADIUN REGENCY Alphania Rahniayu; Gondo Mastutik; Anny Setijo Rahaju; Nila Kurniasari; Khafidhotul Ilmiah; Wira Santoso Ongko
Jurnal Pengabdian Masyarakat Dalam Kesehatan Vol. 4 No. 2 (2022): OCTOBER 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jpmk.v4i2.34741

Abstract

Introduction: Breast cancer is still a burden by being the most frequent malignancy and the highest cause of cancer death in women. The death rate from breast cancer can be reduced by early case finding and appropriate immediate treatment. Early case finding can be started with a simple periodic examination that can be carried out by each individual independently, namely by breast self-examination. This study aims to determine the effect of education on early detection and screening of breast cancer on the level of knowledge among women in Gandul village, Madiun district. Methods: This study involved 101 participants aged from 25 to 87 years old, including female residents of Gandul village and cadres of public health center.  We conduct health education about breast cancer and early detection of breast cancer, especially regarding breast self-examination. Pre-test and post-test were also conducted to assess the the knowledge of the participants before and after the seminar held. Results: The pre-test score of participants with low scores (<75) was 78.21% (79 people) and decreased in the post-test to 43.56% (44 people), while participants with high scores (>75) were only 21.78% (22 people) in the pre-test and increased to 56.44% (57 people) in the post-test. Conclusion: There is a knowledge improvement about the early detection of breast cancer among participants after the intervention. The knowledge improvement can be seen through the increased score from pre-test to post-test.
EARLY DETECTION OF CERVICAL CANCER AND PAP SMEAR ADEQUACY AT PUSKESMAS PILANGKENCEN, MADIUN DISTRICT Anny Setijo Rahaju; Alphania Rahniayu; Nila Kurniasari; Dyah Fauziah; Etty Hary Kusumastuti; Gondo Mastutik; Dewi Sartika A. W.; Fira Soraya; Khafidhotul Ilmiah
Jurnal Layanan Masyarakat Vol. 7 No. 1 (2023): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v7i1.2023.100-113

Abstract

This study aims to increase understanding about early detection of cervical cancer and pap smear evaluation among residents, as well as to increase the pap smear sampling skills by health workers in Pilangkenceng District, Madiun Regency. This study was a descriptive observational study using a cross-sectional approach which was conducted on 100 women, from the Pilangkenceng Primary Health Center in Madiun Regency, aged 20 to 70 years. Pretest and post test assessments were carried out before and after the education of cervical cancer. Specimen adequacy assessment was carried out on 100 Pap smear samples taken by the health workers based on the Papaniculaou classification and the Bethesda system. Pap smear results were also compared with the results of the visual inspection of acetic acid (IVA) which was done before. Education of cervical cancer to the community can increase people's understanding of cervical cancer by 8.05% to 82.26%, while training for health workers on the technique of pap smear sampling shows an increase in knowledge of 51.62% to 100%. Adequacy of pap smear samples by health workers in Pilangkenceng District, Madiun Regency showed that 99% were adequate and satisfactory. Ten percent of IVA examinations showed positive results and those cases showed signs of inflammation and infection on pap smear examination. Community service activities of early detection of cervical cancer through the pap smear method can increase the understanding of early detection of cervical cancer in the community and the knowledge and sampling skills of health workers, hence the health workers at the Pilangkenceng Primary Health Center are ready to provide pap smear services for the community.   Keywords: adequacy, early detection, pap smear, IVA, cervical cancer.   Abstrak Kegiatan ini bertujuan untuk mengetahui peningkatan pemahaman tentang deteksi dini kanker serviks dan serta evaluasi pap smear pada warga, serta peningkatan kemampuan dalam melakukan pap smear oleh tenaga kesehatan di Kecamatan Pilangkenceng Kabupaten Madiun. Penelitian observasional deskriptif dilakukan dengan metode pendekatan cross sectional yang dilakukan pada 100 wanita, dari wilayah Puskesmas Pilangkenceng Kabupaten Madiun, dengan usia 20-70 tahun. Penilaian pretest dan post test dilakukan sebelum dan sesudah penyuluhan. Penilaian adekuasi spesimen dilakukan pada 100  sediaan  pap smear  yang diambil oleh para tenaga berdasarkan klasifikasi Papaniculaou dan sistem Bethesda. Hasil pap smear juga dibandingakan dengan hasil pemeriksaan inspeksi visual asam asetat (IVA) yang dilakukan sebelumnya. Penyuluhan terhadap masyarakat dapat meningkatkan pemahaman masyarakat tentang kanker serviks sebesar 8,05% menjadi 82,26%, sedangkan pelatihan pada tenaga kesehatan tentang tehnik pengambilan pap smear menunjukkan peningkatan pengetahuan sebesar 51,62% menjadi 100%. Adekuasi sediaan pap smear oleh tenaga kesehatan Kecamatan Pilangkenceng Kabupaten Madiun menunjukkan bahwa 99% adekuat dan memuaskan. Sepuluh persen pemeriksaan IVA menunjukkan hasil positif  dan setelah dilakukan hapusan  pap smear, kasus IVA positif tersebut menunjukkan gambaran proses keradangan dan infeksi. Kegiatan pengabdian masyarakat dapat meningkatkan pemahaman tentang deteksi dini keganasan serviks pada masyarakat dan pengetahuan para tenaga kesehatan tentang deteksi dini kanker serviks melalui metode pap smear, sehingga diharapkan bahwa tenaga kesehatan di Puskesmas Pilangkenceng siap memberikan pelayanan pap smear bagi warga. Kata kunci: adekuasi, deteksi dini, pap smear, IVA, kanker serviks.
Classification of endometrial adenocarcinoma using histopathology images with extreme learning machine method Rulaningtyas, Riries; Rahaju, Anny Setijo; Dewi, Rosa Amalia; Hanifah, Ummi; Purwanti, Endah; Rahma, Osmalina Nur; Katherine, Katherine
Indonesian Journal of Electrical Engineering and Computer Science Vol 36, No 2: November 2024
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijeecs.v36.i2.pp961-971

Abstract

As many as 70-80% of endometrial cancer cases are endometrial adenocarcinoma. Histopathological assessment is based on the degree of differentiation, into well-differentiated, moderate-differentiated, and poorly-differentiated. Management and prognosis differ between grades, so differential diagnosis in determining the degree of tumor differentiation is crucial for appropriate treatment decisions. Histopathological image analysis offers detailed diagnostic results, but manual analysis by a pathologist is very complicated, error-prone, quite tedious, and time-consuming. Therefore, an automatic diagnostic system is needed to assist pathologists in grading the tumor. This research aims to determine the degree of differentiation of endometrial adenocarcinoma based on histopathological images. The extreme learning machine (ELM) method performs image classification with gray level run long matrix (GLRLM) features and a combination of local binary pattern (LBP)-GLRLM features as input. Experimental results show that the ELM model can achieve satisfactory performance. Training accuracy, testing accuracy, and model precision with GLRLM features were 97.13%, 91.33%, and 80% and combined LBPGLRLM features were 91.03%, 71.33%, and 100%. Overall, the model created can determine the degree of tumor differentiation and is useful in providing a second opinion for pathologists.
EDUCATION ABOUT BREAST CANCER IN THE COMMUNITY AT NGLUYU HEALTH CENTER, NGANJUK Mastutik, Gondo; Fauziah, Dyah; Rahaju, Anny Setijo; Rahniayu, Alphania; Ridholia; Kurniasari, Nila; Kusumastuti, Etty Hari; Ilmiah, Khafidhotul
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 8 No. 3 (2024): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v8i3.2024.422-431

Abstract

Breast cancer is the most common cancer found in the world and this cancer is the second cause of death due to cancer after lung cancer in Indonesia. This high mortality rate can be prevented by carrying out early detection. However, this is still not done well, especially in communities living in remote areas with low to medium economic and educational levels. The aim is to convey knowledge about breast cancer and training in the self-examination of the breast. It was held on Tuesday, 12 September 2023 at the Ngluyu Community Health Center, Nganjuk, from 07.00 to 14.00 WIB and was attended by 111 people. Data on breast cancer risk factors was collected through questionnaires. There were 96 participants who filled out the questionnaire, aged between 22-63 years. Risk factors data showed that 3.13% did not have children, 15.63% breastfed children less than 6 months, 5.21% were exposed to X-rays more than once a year; 1.04% had breast tumors, and 4.17% had family members suffering from cancer. In addition, 89.58% were over 25 years old and 42.71% had doing self-examination of breast. There was an increase in public understanding at the Ngluyu Community Health Center from 77.70% to 84.12%, namely an increase of 6.42%. This can increase public understanding about breast cancer and procedures to self-examination of breast and contribute in early detection of breast cancer as well as reducing the incidence of breast cancer. The results of this counseling can be used as recommendations by health centers in the Nganjuk district area.
SHORT-TERM TOXICITY TEST OF SINGALAWANG EXTRACT ON MALE WHITE RATS KIDNEY NECROSIS Naibaho, Ardy Hamonangan; Mustika, Arifa; Rahaju, Anny Setijo
Folia Medica Indonesiana Vol. 52 No. 1 (2016): JANUARY - MARCH 2016
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.353 KB) | DOI: 10.20473/fmi.v52i1.5212

Abstract

Singawalang leaf is used as a traditional medicine by the communities suffering from pneumonia due to TB disease. However, experiments to explain it's toxicity are few. Therefore, this study was conducted to disclose it's toxicity. This was an experimental laboratory study using post–test only control group design. The sampling method was simple random sampling. The animals were randomly divided into 4 (four) groups, each group consisted of 8 (eight) mice, the control group (P0) received distilled water as a solvent material. Treatment group 1 (P1) obtained ethanol extract of singawalang (Petiveria alliaceae) in low doses of 90 mg/kgbw. Treatment group 2 (P2) obtained ethanol extract of singawalang (Petiveria alliaceae) in medium doses of 180 mg/kgbw. Treatment group 3 (P3) obtained ethanol extract of singawalang (Petiveria alliaceae) in high doses of 360 mg/kgbw. Th extracts were given with sonde once a day for 30 days. Results showed that the leaf ethanol extract singawalang (Petiveria alliaceae) does not induce sub-acute necrosis effects on kidney of male white mice (Mus musculus) in doses 90 mg/kgbw, 180 mg/kgbw, and 360 mg/kgbw.
Co-Authors Abdurrahman Hasyim Asy’ari Alphania Rahniayu Aniek Meidi, Aniek Aniek Meidy Utami Ari Wanda, Dewi Sartika Ariadna Anggi Pasang Ariani, Grace Arifa Mustika Arifa Mustika Aulia Nur Fadila Budi Harjanto Dewi Sartika A. W. Dewi, Rosa Amalia DWI SURYANTO DYAH FAUZIAH, DYAH Eddie Saputra Ferdinant Martinus Djawa Fira Soraya Gondo Mastutik Hadiyyatan Waasilah HERAWATI, LILIK Heriyawati, Heriyawati Heryanto Heryanto I'tishom, Reny Ilmiah, Khafidhotul Imam Susilo Isnin Anang Marhana Izzan Khalidah Binti Muhamad Katherine Kho Khafidhotul Ilmiah Khafidhotul Ilmiah Krismaningrum, Veronika Intan Kusumastuti , Etty Hary Kusumastuti, Etty Hari KUSUMASTUTI, ETTY HARY Leonita Agustin Hambalie Linda Dewanti Mas`udi, Achmad Fayyad Naibaho, Ardy Hamonangan Nila Kurniasari Nila Kurniasari Novalia Chumaladewi Guntarno Osmalina Nur Rahma PUNGKY MULAWARDHANA, PUNGKY Qonitatillah, Ana Rahmi Alia Rahmi Alia Rahmi Alia, Rahmi Renny I’tishom Reza Wangsanagara Ridholia Ridholia Ridholia Ridholia, Ridholia Riries Rulaningtyas Roostantia Indrawati S.Pd. M Kes I Ketut Sudiana . Salman Aziz, Muhamad Abi Sari, Aditya Sita Sheilla Matheos Sita Sari, Aditya Sjahjenny Mustokoweni, Sjahjenny Soetojo Soetojo Solly Aryza Suhartono Taat Putra Tarmono Djojodimedjo Tri Wulanhandarini Trianto, Heru Fajar Tuti Andayani Ummi Hanifah Vicky Sumarki Willy Sandhika Winarno, Winarno Wira Santoso Ongko Wiratama, Priangga Adi Yohana Octavianda