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Journal : agriTECH

Pediococcus acidilactici F-11 Penghasil Bakteriosin sebagai Agensia Biokontrol Escherichia coli dan Staphylococcus aureus pada Sayuran Segar Simpan Dingin Endang Sri Rahayu; Eni Harmayani; Tyas Utami; Kejora Handarini
agriTECH Vol 24, No 3 (2004)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2344.649 KB) | DOI: 10.22146/agritech.13456

Abstract

Our survey indicated that fresh vegetables contained. high population of microorganisms including pathogenic Staphylococcus aureus. Washing procedures, including the addition of sanitizes to the wash water have not been effectively in reduction of the number of microorganisms. Currently, there is interest in possible use bacteriocin producer of lactic acid bacteria as biocontrol agents to ensure safety of minimally processed, refrigerated (MPR) foods which are not acidified, including fruits and vegetables. Our previous result, indicated that Pediococcus acidilactici F-11 (PAF-11) produced bacteriocin with wide spectrum activity. Objectives of this research was to study the potency of PAF-I 1 as biocontrol agent to inhibit the growth of pathogenic bacteria occurred in ready to eat fresh vegetables, i.e., paprika, lettuce, carrot. The results showed that PAF-11 was able to inhibit the growth of naturally present coliform and Staphylococcus significantly, as well as that of tested bacteria of E. coil and S. aureus which were inoculated into paprika and carrot. PAF-11 was able to grow at these two vegetables, and their population were increased about I log cycle. Bacteriocin activity produced by PAF-11 was positively detected from these two vegetables inoculated with these bacteria. However, there was no inhibition activity of PAF-11 against naturally present coliform and Staphylococcus, as well as inoculated E. coil and S. aureus on lettuce. Bacteriocin activity produce by PAF-11 was also not detected in this vegetables. Conclusion of this study, Pediococcus acidilactici F-11 could be used as biocontrol agents in paprica and carrot.
Effect of Withering and Drying Process of Cemara Udang (Casuarina equisetifolia) Leaves to Functional Herbal Tea Handarini, Kejora; Rahmiati, Retnani; Yuniati, Yuyun
agriTECH Vol 45, No 1 (2025)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.94351

Abstract

The industry is intensively producing health product from herbal plants to combat free radicals and prevent the potential of cancer disease in the body. In Indonesia, Cemara Udang (Casuarina equisetifolia) leaves have been investigated as herbal tea product for antioxidant purposes as a free radical scavenger from various plants. This herbal tea requires significant attention during processing to produce a quality final product. Therefore, this research aimed to examine the effect of setting withering times of 14 and 16 hours, along with a drying temperature of 40 and 50 °C on cemara udang leaves. Product quality was evaluated based on the chemical components and nutritional characteristics of tea, such as moisture content, pH, antioxidant activity, total phenolic content, and vitamin C, as well as sensory aspects. The evaluation was carried out based on the degree of preference for color, aroma, and taste. Fresh cemara udang samples had 46.57% moisture content, pH 7.05, 131.53 µg/mL IC50 value, 1.53 mg/100 g total phenolic, and 43.46% vitamin C. Based on the degree of preference, color, flavor, and taste hedonic score had 3.11, 2.57, and 1.94 out of 7.00, respectively. Tea of cemara udang that was withered for 16 hours and dried at 50°C showed good quality stability with favorable sensory attributes. This varies significantly compared to the control, with 10.32% moisture content, pH 7.04, 137.94 µg/mL IC50 value, 1.2 mg/100 g total phenolic, and 29.26% vitamin C. Furthermore, color, flavor, and taste hedonic scores had 6.37, 6.03, and 6.27 out of 7.00, respectively.
Co-Authors Achmad Kusyairi Achmad Kusyairi Adiansah, Amanda Aprilia Andi Athifa Putri Aprilianto, Richardus Arlin Besari Djauhari Arlin Besari Djauhari Aryani, Amalia Dwi Bambang Sigit Sucahyo Bambang Sigit Sucahyo Cahyani Tri Susilo, Regitha Pandu Chasanah, Dwi Asmaul Christian Theodorus, Fransiskus Devi, Yuni Astika Diana Puspitasari Didik Budiyanto Didik Trisbiantoro Djauhari, Arlin Besari Endang Retno Wedowati Endang Sri Rahayu Eni Harmayani Fadjar Kurnia Hartati, Fadjar Kurnia Falesta, Pasyaura Ramadhani Fungki Sri Rejeki Gultom, Sinta Febriyanti Husnul Khotimah Jannah, Jumrotul Jehandu, Maria Yuliana Permatasari Karimullah, RB. Moh. Kasmiati Kasmiati Kurnia Hartati, Fadjar Kusyairi, Achmad Madyowati, Sri Oetami Mekasari, Firsta Koesdyah Metusalach Metusalach Ningtyas, Rosidah Wahyu Nirmala Siregar, Rena Nunuk Hariani Nunuk Hariyani Nunuk Hariyani Nunuk Hariyani Nurfaidah Nurfaidah Nursakinah Latuconsina Nurul Hayati NURUL HAYATI Ophirtus Sumule Orodiputro, Charolin Prajudanti, Adhania Andika Pramitha, Asti Rizkiana Prayudanti, Adhania Andika Rahmatang Rahmatang Rahmatul Khasanah Rahmiati, Retnani Ramadhani, Mirza Retnani Rahmiati Retnati Rahmiati Ricky Indra Nur Pratama Saraswati, Exist Siregar, Rena Nirmala Sri Oetami Madyowati Sri Oetami Madyowati Sri Oetami Madyowati Sucahyo, Bambang Sigit Sumaryam Sumaryam Sumaryam Suriana Laga Tajuddin Noor, M. Tirtawan, Titus Trisbiantoro, Didik Tyas Utami Wa Ode Nurul Azizah Nida Ul Haq Yudo, Wandoko Sungkowo Yuliani, Novia Dwi Yuyun Yuniati Yuyun Yuniati Yuyun Yuniati