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Ika Trisharyanti Dian Kusumowati
Universitas Muhammadiyah Surakarta
Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : Biomedika

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DAYA ANTIBAKTERI EKSTRAK ETANOL DAUN SENGGANI (Melastoma affine D. Don) Kusumowati, Ika Trisharyanti Dian; Melannisa, Rosita; Prasetyawan, Angga
Biomedika Vol 6, No 2 (2014): Biomedika Agustus 2014
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

Melastoma affine D. Don had some activities such as anthelmintic, antibacteria, antiinfiammation, antifungal, and antitumor. The aims of this research was determine antibacteria activity of ethanolic extract of Melastoma affine D. Don. The antimicrobial activity was tested by solid dilution method to get Minimum Inhibition Concentration (MIC). The compounds in Melastoma affine D. Don was analyzed by tube test method and Thin Layer Chromatography (TLC) with chloroform : methanol : formic acid (8,5:1,5:0,5) as mobile phase and silica gel GF254 as stationary phase. The result showed ethanolic extract of Melastoma affine D. Don contains alkaloid, polyphenol, fiavonoid, saponin, and essential oil. The MIC of Senggani against Staphylococcus aureus was 2% and 3% against Escherichia coli and the extract could not inhibit Staphylococcus aureus and Escherichia coli multiresistant until concentration 7% extract ethanol.Keywords: Melastoma affine D. Don, Staphylococcus aureus, Escherichia coli
KORELASI KANDUNGAN FENOLIK DAN AKTIVITAS ANTIOKSIDAN DAUN JAMBU METE Kusumowati, Ika Trisharyanti Dian; Melannisa, Rosita; Ratri, Kartikaning
Biomedika Vol 3, No 2 (2011): Biomedika Agustus 2011
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

The ethanolic extract of Anacardium occidentale L. leaves contains phenolic compounds,  avonoids, alkaloids, saponins, tannins, and steroids that could have antioxidant activity. The aim of this research was to determine the correlation between the antioxidant activity and the phenolic compounds of the ethanol extract of Anacardium occidentale L. leaf. The DPPH methode was used to determine the antioxidant activity. The total phenolic determined using Folin-Ciocalteu reagent. There was a positive correlation between antioxidant activity and total phenolic content in the ethanolic extract of cashew leaf with correlation coef cient R2 = 0.5888.Keywords: Anacardium occidentale L., antioxidant, phenolic compounds
ANTIOKSIDAN EKSTRAK ETANOL 70% DAUN SYZYGIUM POLYANTHUM (WIGHT) (SALAM) SECARA INVITRO Sutrisna, EM; Trisharyanti, Ika; Munawaroh, Rima; ., Suprapto
Biomedika Vol 7, No 1 (2015): Biomedika Februari 2015
Publisher : Universitas Muhamadiyah Surakarta

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Abstract

Radikal bebas merupakan satu faktor kontributor terjadinya penyakit degenerative. Antioksidan merupakan senyawa yang menghambat efek radikal bebas tersebut. Pada penelitian sebelumnya, ekstrak methanol daun Daun salam mempunyai efek antioksidan. Penelitian ini bertujuan menguji efek antioksidan ekstrak ethanol 70% daun salam (Syzygium polyanthum (Wight)). Metode yang digunakan adalah uji invitro dengan metode DPPH (Diphenyl picrylhydrazyl). Ekstrak dilarutkan dalam ethanol, kemudian dibuat dalam seri konsentrasi 10, 30, 50 dan 70 ug/mL. Masing-masing seri sebanyak 10 ml. Dalam masing-masing larutan tersebut ditambahkan 1 ml larutan DPPH 1 mM. larutan kemudian diinkubasi pada suhu 37°C selama 30 menit. Panjang gelombang yang digunakan adalah 515 nm. Metanol dan DPPH 1mM digunakan sebagai blanko.Untuk pembanding digunakan butylated hydroxytoluene (BHT) konsentrasi 2, 4, 6, 8 ug/mL. Hasil penelitian menunjukkan bahwa ekstrak ethanol 70% daun salam mempunyai efek antioksidan dengan IC 50 27,80 ug/mL.Kata kunci: daun salam (Syzygium polyanthum (Wight) , antioksidan, IC 50
KORELASI KANDUNGAN FENOLIK DAN AKTIVITAS ANTIOKSIDAN DAUN JAMBU METE Kusumowati, Ika Trisharyanti Dian; Melannisa, Rosita; Ratri, Kartikaning
Biomedika Vol 3, No 2 (2011): Biomedika Agustus 2011
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v3i2.251

Abstract

The ethanolic extract of Anacardium occidentale L. leaves contains phenolic compounds, ? avonoids, alkaloids, saponins, tannins, and steroids that could have antioxidant activity. The aim of this research was to determine the correlation between the antioxidant activity and the phenolic compounds of the ethanol extract of Anacardium occidentale L. leaf. The DPPH methode was used to determine the antioxidant activity. The total phenolic determined using Folin-Ciocalteu reagent. There was a positive correlation between antioxidant activity and total phenolic content in the ethanolic extract of cashew leaf with correlation coef? cient R2 = 0.5888.Keywords: Anacardium occidentale L., antioxidant, phenolic compounds
DAYA ANTIBAKTERI EKSTRAK ETANOL DAUN SENGGANI (Melastoma affine D. Don) Kusumowati, Ika Trisharyanti Dian; Melannisa, Rosita; Prasetyawan, Angga
Biomedika Vol 6, No 2 (2014): Biomedika Agustus 2014
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v6i2.278

Abstract

Melastoma affine D. Don had some activities such as anthelmintic, antibacteria, antiinfiammation, antifungal, and antitumor. The aims of this research was determine antibacteria activity of ethanolic extract of Melastoma affine D. Don. The antimicrobial activity was tested by solid dilution method to get Minimum Inhibition Concentration (MIC). The compounds in Melastoma affine D. Don was analyzed by tube test method and Thin Layer Chromatography (TLC) with chloroform : methanol : formic acid (8,5:1,5:0,5) as mobile phase and silica gel GF254 as stationary phase. The result showed ethanolic extract of Melastoma affine D. Don contains alkaloid, polyphenol, fiavonoid, saponin, and essential oil. The MIC of Senggani against Staphylococcus aureus was 2% and 3% against Escherichia coli and the extract could not inhibit Staphylococcus aureus and Escherichia coli multiresistant until concentration 7% extract ethanol.Keywords: Melastoma affine D. Don, Staphylococcus aureus, Escherichia coli
KORELASI KANDUNGAN FENOLIK DAN AKTIVITAS ANTIOKSIDAN DAUN JAMBU METE Ika Trisharyanti Dian Kusumowati; Rosita Melannisa; Kartikaning Ratri
Biomedika Vol 3, No 2 (2011): Biomedika Agustus 2011
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v3i2.251

Abstract

The ethanolic extract of Anacardium occidentale L. leaves contains phenolic compounds,  avonoids, alkaloids, saponins, tannins, and steroids that could have antioxidant activity. The aim of this research was to determine the correlation between the antioxidant activity and the phenolic compounds of the ethanol extract of Anacardium occidentale L. leaf. The DPPH methode was used to determine the antioxidant activity. The total phenolic determined using Folin-Ciocalteu reagent. There was a positive correlation between antioxidant activity and total phenolic content in the ethanolic extract of cashew leaf with correlation coef cient R2 = 0.5888.Keywords: Anacardium occidentale L., antioxidant, phenolic compounds
DAYA ANTIBAKTERI EKSTRAK ETANOL DAUN SENGGANI (Melastoma affine D. Don) Ika Trisharyanti Dian Kusumowati; Rosita Melannisa; Angga Prasetyawan
Biomedika Vol 6, No 2 (2014): Biomedika Agustus 2014
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v6i2.278

Abstract

Melastoma affine D. Don had some activities such as anthelmintic, antibacteria, antiinfiammation, antifungal, and antitumor. The aims of this research was determine antibacteria activity of ethanolic extract of Melastoma affine D. Don. The antimicrobial activity was tested by solid dilution method to get Minimum Inhibition Concentration (MIC). The compounds in Melastoma affine D. Don was analyzed by tube test method and Thin Layer Chromatography (TLC) with chloroform : methanol : formic acid (8,5:1,5:0,5) as mobile phase and silica gel GF254 as stationary phase. The result showed ethanolic extract of Melastoma affine D. Don contains alkaloid, polyphenol, fiavonoid, saponin, and essential oil. The MIC of Senggani against Staphylococcus aureus was 2% and 3% against Escherichia coli and the extract could not inhibit Staphylococcus aureus and Escherichia coli multiresistant until concentration 7% extract ethanol.Keywords: Melastoma affine D. Don, Staphylococcus aureus, Escherichia coli
ANTIOKSIDAN EKSTRAK ETANOL 70% DAUN SYZYGIUM POLYANTHUM (WIGHT) (SALAM) SECARA INVITRO. EM Sutrisna; Ika Trisharyanti; Rima Munawaroh; Suprapto .
Biomedika Vol 7, No 1 (2015): Biomedika Februari 2015
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v7i1.1584

Abstract

Radikal bebas merupakan satu faktor kontributor terjadinya penyakit degenerative. Antioksidan merupakan senyawa yang menghambat efek radikal bebas tersebut. Pada penelitian sebelumnya, ekstrak methanol daun Daun salam mempunyai efek antioksidan. Penelitian ini bertujuan menguji efek antioksidan ekstrak ethanol 70% daun salam (Syzygium polyanthum (Wight)). Metode yang digunakan adalah uji invitro dengan metode DPPH (Diphenyl picrylhydrazyl). Ekstrak dilarutkan dalam ethanol, kemudian dibuat dalam seri konsentrasi 10, 30, 50 dan 70 ug/mL. Masing-masing seri sebanyak 10 ml. Dalam masing-masing larutan tersebut ditambahkan 1 ml larutan DPPH 1 mM. larutan kemudian diinkubasi pada suhu 37°C selama 30 menit. Panjang gelombang yang digunakan adalah 515 nm. Metanol dan DPPH 1mM digunakan sebagai blanko.Untuk pembanding digunakan butylated hydroxytoluene (BHT) konsentrasi 2, 4, 6, 8 ug/mL. Hasil penelitian menunjukkan bahwa ekstrak ethanol 70% daun salam mempunyai efek antioksidan dengan IC 50 27,80 ug/mL.Kata kunci: daun salam (Syzygium polyanthum (Wight) , antioksidan, IC 50
Co-Authors Afifah Mujahidah Shalihah Ahya Nafila Fatihati Ajeng, Jessica Sastry Amanda Wahyu Kurniawan Ambar Yunita Ambar Yunita Nugraheni Andi Suhendi Andieni , Herdania Puan Angga Prasetyawan Angga Prasetyawan, Angga Anggraeni, Safitri Nur Anindya, Talitha Ahnaf Anita Sukmawati Anjati, Yasinta Widia Arifah Sri Wahyuni Arina Nisyya Nadhira Asmitha, Suci Miranda Asti Arum Sari Astrina Dewi Ratnaningrum Astrina Dewi Ratnaningrum, Astrina Dewi Audi Tahta Aurellia Azistyna, Delvi Yulia Balqis, Moetia Rahman Brainandiva Ade Fitria Broto Santoso Cahya Rahma Utami Cholisoh, Zakky Dedi Hanwar Dian Kusumowati Elma Arifatul Sugito Em Sutrisna Endang Setyaningsih Erindyah Retno Wikantyasning Firdaus, Farhand Ahmad Fransisda Aulia Zatsyia Hamidah, Ummi Harindasari, Amorita Azzah Heppy Purbasari Isnaniyah, Dhevi Zahra Latifatul Jessica Sastry Ajeng Kartikaning Ratri Kartikaning Ratri, Kartikaning Kasful Asra Sakika Khoirunnisa Khoirunnisa Larasati, Arindra Lestari, Kurnia Livia Purwati Luluk Ria Rakhma Marcellino Rudyanto Merlindasari, Aulia Pungky Muhammad Da'i Muhammad Ikhsan Al Af Ghani Nazilah, Siti Umi Nobela, Deandra Nur Chasanah Peni Indrayudha Purwati, Livia Rahmadani, Sandika Rahmatillah, Annie Ramadhani Dwi Sula Ramdani, Tasya Eka Refsya Azanti Putri Rima Munawaroh Rosita Melannisa Rosita Melannisa Rosita Melannisa, Rosita Sacharina, Dewi Safira Maharani Sania Nayasari Khoirunnisa Septiani, Adiva Metri Setyo Nurwaini Shofiah, Mira Siswandono, Siswandono Siti Umi Nazilah Sukamto, Dandi Febrian Suprapto . Suprapto . Tanti Azizah Sujono Triyatno, Sabrina Azizah Ujiantya, Sabrina Dian Parasika Ulil Fikri Nurhakimah Ummi Hamidah UTARI SITA ARDANI Wahyu Utami Wahyu Utami, Wahyu Wahyuningsih, Putri Dewi Wanudya Atmajani Wati, Abil Lia Yuhdi, Fajrin Ahidannisa Yuka Aulia Rahma YULIANA RIZQI DWI RATNA ZAKIAH FATHIANA