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Kandungan Pigmen dan Aktivitas Antioksidan pada Tanaman Celosia plumosa Bunga Merah dan Kuning Dewi Prabawaningrum; Sri Kasmiyati; Anggara Mahardika
Buletin Anatomi dan Fisiologi Volume 5, Nomor 2, Tahun 2020
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.5.2.2020.119-128

Abstract

Tanaman Celosia plumosa memiliki variasi warna yang ditentukan oleh kandungan pigmen. Aktivitas antioksidan memiliki senyawa yang mampu memperlambat proses oksidasi. Penelitian ini bertujuan untuk mengetahui kandungan pigmen (klorofil, karotenoid, dan antosianin), flavonoid, fenolik, dan aktivitas antioksidan pada tanaman C.plumosa bunga merah dan kuning dengan variasi organ daun dan bunga. Analisis kandungan klorofil dan karotenoid dengan spektrofotometri, antosianin dengan pH-diferensial, flavonoid dengan AlCl3(kolorimetri), fenolik dengan Folin-ciocalteau, dan aktivitas antioksidan dengan DPPH. Kandungan klorofil tertinggi pada daun bunga kuning sebesar 128,80 µg/ml. Kandungan karotenoid tertinggi pada bunga merah sebesar 8,27 mg/ml. Kandungan antosianin tertinggi pada bunga merah sebesar 28,94 mg/gr. Flavonoid tertinggi pada bunga merah sebesar 5,60 mg/L. Fenolik tertinggi pada bunga merah sebesar 3,48 mg/L. Nilai IC50 tertinggi ditemukan pada bunga merah sebesar 2,75 ppm. Bunga merah memiliki kandungan pigmen antosianin dan karotenoid yang tinggi, dan didukung dengan metabolit sekunder flavonoid dan fenolik yang tinggi. Pada bunga merah kandungan pigmen karotenoid dengan flavonoid terdapat korelasi positif. Sedangkan pada daun bunga kuning dan merah tidak ada korelasi antara flavonoid dan pigmen. Pada daun bunga kuning terdapat korelasi positif antara pigmen antosianin dengan flavonoid yang kuat. Kata kunci : Antioksidan, Celosia plumosa, Flavonoid, Fenolik, Pigmen.
Students' understanding of plant classification using the PLANTA-FUN card game media Debora Kristiani Rahardjo; Wahyu Hari Kristiyanto; Sri Kasmiyati
JURNAL BIOEDUKATIKA Vol 9, No 3 (2021)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26555/bioedukatika.v9i3.20535

Abstract

UNO card game-based learning media have been developed with various modifications. Therefore, there is a need for studies that describe the role and meaning of the symbols used in UNO card media on students' understanding. The purpose of this study is to describe the profile of students' understanding who were stimulated by symbols of meaning in the PLANTA-FUN card game media on plant classification material. The PLANTA-FUN card game media is a modification of the UNO card game created by combining the colors and images of the key-living creatures. This research was conducted using Mixed Method Research (quantitative-qualitative). Pre-test and post-test to determine students' understanding of classifying the plants. In addition, interviews were also conducted to evaluate students' understanding of the meaning of the symbols. Based on the pre-test and post-test results, the t-value (8.612) > the t-table (2.101). So it can be seen that there was an increase in students' understanding after learning using the PLANTA-FUN card game media. Based on the interview, symbols of meaning in the form of images and colors on the PLANTA-FUN card game media can help stimulate students to remember and think. The key character images of plants used in this media can naturally visualize plants so that students can quickly identify the characteristics that appear in these plants. At the same time, the color symbols that represent groups of living things can quickly stimulate students in recalling groups of plants. Combining these two symbols of meaning can improve students' understanding of plant classification learning material.
Kadar Flavonoid, Senyawa Biomarker Antikanker pada Tumbuhan Famili Asteraceae dari Daerah Kopeng Kabupaten Semarang Indonesia Elizabeth Betty Elok Kristiani; Sri Kasmiyati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 37, No 1 (2020)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (748.245 KB) | DOI: 10.20884/1.mib.2020.37.1.1058

Abstract

The Asteraceae family plants are reported to have various abilities as drugs. Artemisia belongs to the Asteraceae family has been used as a drug for malaria, and recent developments have been studied for its ability as anticancer agents. Cosmos caudatus, a member of the Asteraceae family that believed by many people for its ability to heal cancer. The aims of this study were to determine the content of quercetin, kaempferol, and artemisinin which is an anticancer biomarker compound in A. annua, A. vulgaris Linn., A. chinese Berg ex Poljakov, and Cosmos caudatus Kunth which grow in Kopeng blood, Semarang Regency, Province Central Java. The plant samples were dried and mashed up. The content of biomarker compounds was determined using using High Performance Liquid Chromatography (HPLC). The artemisinin levels in A. annua, A. vulgaris Linn., A. cina Berg Poljakov, and Cosmos caudatus Kunth were 0.2800; 0.3000; 0.1700; and 0.0300% respectively. The kaempferol was detected in the three Artemisia were 0.2987; 0.0013; and 0.0074%. In all four plants, quercetin was only detected in A. annua.
THE APPLICATION OF Fe AND Cr(III) IN GROWING MEDIA AND ITS EFFECT ON PLANT GROWTH AND Cr(III) OXIDATION ON Tagetes erecta Penina Intansari; Sri Kasmiyati; . Sucahyo
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022): June 2022
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (766.085 KB) | DOI: 10.29122/jbbi.v9i1.4865

Abstract

The oxidation of Cr(III) to Cr(VI) in the environment has a detrimental impact because it can change the form of non-toxic Cr(III) to Cr(VI), which is toxic to organisms. The study aimed to examine the effect of the application of iron (Fe) and trivalent chromium (Cr(III)) compounds in Tagetes erecta growing media on growth and Cr(III) oxidation. Concentrations of Cr(III) 0, 100, and 500 mg/L and Fe 0, 3, 15, and 30 mg/L were applied to the growing media of T. erecta as the model plant. The growth and accumulation of Cr(VI) in plants were measured to determine the effect of Fe and Cr(III) treatment on growth and Cr(III) oxidation. The accumulation of Cr(VI) in the roots and shoots of T. erecta increased significantly due to the addition of Fe in the growing media treated with Cr(III). The highest accumulation of Cr(VI) in the roots and shoots of T. erecta found in the treatment of Cr(III) 500 mg/L and Fe 30 mg/L, were respectively 0.092 g/L and 0.070 g/L. The addition of Fe in growing media containing Cr(III) increased plant height, root length, and shoot dry weight but decreased leaf number and root dry weight. T. erecta root biomass was more affected by the toxic impact of Fe than Cr(III). On the other hand, the inhibition of leaf formation was caused by the toxic effect of Cr(III) rather than Fe. Oksidasi Cr(III) menjadi Cr(VI) di lingkungan mempunyai dampak merugikan, karena dapat mengubah bentuk Cr(III) dari tidak toksik menjadi bentuk Cr(VI) yang toksik bagi organisme. Tujuan penelitian adalah untuk mengkaji efek aplikasi senyawa besi (Fe) dan kromium trivalen (Cr(III)) dalam media tanam Tagetes erecta terhadap pertumbuhan dan oksidasi Cr(III). Konsentrasi Cr(III) 0, 100 dan 500 mg/L dan Fe 0, 3, 15 dan 30 mg/L diaplikasikan dalam media tanam T. erecta sebagai tanaman uji. Pertumbuhan dan akumulasi Cr(VI) pada tanaman diukur untuk mengetahui efek perlakuan dan mendeteksi terjadinya oksidasi Cr(III). Akumulasi Cr(VI) pada akar dan pucuk T. erecta mengalami peningkatan secara nyata akibat penambahan Fe dalam media tanam yang diberi perlakuan Cr(III).  Akumulasi Cr(VI) pada akar dan pucuk T. erecta tertinggi dijumpai pada perlakuan Cr(III) 500 mg/L dan Fe 30 mg/L berturut-turut adalah 0,092 g/L dan 0,070 g/L. Penambahan Fe dalam media tanam mengandung Cr(III) meningkatkan pertumbuhan tinggi tanaman, panjang akar dan berat kering pucuk, namun menurunkan jumlah daun dan berat kering akar. Biomassa akar T. erecta lebih dipengaruhi oleh efek toksik Fe dibandingkan Cr(III), sebaliknya penghambatan pembentukan daun lebih disebabkan oleh efek toksik Cr(III) daripada Fe.
Penghambatan Perkecambahan dan Pertumbuhan Kecambah Tagetes erecta pada Media Mengandung Kromium Heksavalen Juliana Sarioa; Sri Kasmiyati; Susanti Puji Hastuti
Buletin Anatomi dan Fisiologi Volume 7, Nomor 2, Tahun 2022
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.7.2.2022.144-152

Abstract

Perkecambahan biji merupakan fase kritis yang menentukan kelangsungan hidup dan toleransi tanaman pada lingkungan tercemar logam berat. Krom heksavalen (Cr6+) adalah salah satu logam berat yang bersifat toksik bagi tumbuhan. Tujuan penelitian mengetahui efek Cr6+ terhadap perkecambahan biji dan pertumbuhan kecambah Tagetes erecta. Uji perkecambahan biji dan pertumbuhan kecambah dilakukan secara eksperimental menggunakan rancangan acak lengkap dengan 4 perlakuan konsentrasi Cr6+ (K2CrO4) meliputi 0 (kontrol), 5, 25, dan 50 mg/L. Jumlah biji berkecambah diamati setiap hari selama 10 hari dan digunakan untuk menentukan persentase perkecambahan dan indeks vigor kecambah. Pertumbuhan kecambah ditentukan berdasarkan panjang radikula dan panjang epikotil, dan bobot kering kecambah pada akhir penelitian. Cr6+ mempengaruhi secara signifikan perkecambahan dan pertumbuhan kecambah T. erecta.  Konsentrasi Cr6+ sebesar 5, 25 dan 50 mg/L menurunkan secara signifikan (p<0,05) persentase perkecambahan, indeks vigor kecambah, panjang radikula, panjang epikotil, dan bobot kering kecambah. Bobot kering kecambah T. erecta pada perlakuan Cr6+ 25 mg/L meningkat signifikan dibanding kontrol dan perlakuan Cr6+ lainnya, karena meskipun pertumbuhan memanjang epikotil dan radikulanya terhambat, namun diameter epikotil dan radikulanya tumbuh lebih besar dan lebih tebal sehingga mendukung lebih besarnya bobot kering. Hasil penelitian diharapkan mendukung pengembangan potensi dan pemanfaatan T. erecta sebagai agen fitoremediasi Cr. Seed germination is a critical phase that determines plant survival and tolerance in heavy metal polluted environments. Hexavalent chromium (Cr6+) is a heavy metal that is toxic to plants. The aim of this study was to determine the inhibition effect of Cr6+ on the germination and sprout growth of Tagetes erecta. The seed germination and sprout growth tests were carried out experimentally using a completely randomized design with 4 concentrations of Cr6+ (K2CrO4) treatment including 0 (control), 5, 25, and 50 mg/L. The number of seeds that germinated was observed every day for 10 days, used to determine the percentage of germination and seedling vigor index. The sprout growth was determined based on radicle length and epicotyl length, and dry weight of sprouts at the end of the study. Cr6+ significantly affected the germination and sprouts growth of T. erecta. The Cr6+ concentrations of 5, 25 and 50 mg Cr6+/L significantly reduced (p<0.05) germination percentage, seedling vigor index, radicle length, epicotyl length, and wet weight of sprouts. The dry weight of T. erecta sprouts in the Cr6+ 25 mg/L treatment increased significantly compared to the control and other Cr6+ treatments, because although the elongation of the epicotyl and radicle was inhibited, the diameter of the epicotyl and radicle grew larger and thicker thus supporting a greater dry weight. These results are expected to support the potential development and utilization of T. erecta as a phytoremediation agent for Cr. 
PENGARUH Mangan (Mn) TERHADAP OKSIDASI KROMIUM TRIVALEN DAN PERTUMBUHAN Tagetes sp. Margareta Prameswati; Sri Kasmiyati; Sucahyo Sucahyo
BERITA BIOLOGI Vol 21, No 3 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i3.4190

Abstract

Chromium can be found in 2 oxidation states, trivalent chromium (Cr3+) and hexavalent chromium (Cr6+) in environment. Trivalent chromium can be oxidized by manganese oxide to hexavalent chromium (Cr6+). The aim of the study was to determine the effect of Mn on the oxidation of Cr³⁺and plant growth of Tagetes. The study was conducted experimentally using a completely randomized design. The Tagetes plants grown in media with 2 treatment factors, namely Cr3+ (CrCl3) with 3 concentrations of 0, 100 and 500 mg/l) and Mn (MnSO4) with 4 concentrations of 0, 1, 5 and 10 mg/l. The plant growth and Cr6+ content in roots and shoots were measured to determine the effect and to detect the occurrence of Cr3+ oxidation. The addition of Mn in the media of Tagetes containing Cr3+ increased the Cr3+ oxidation process, indicated by the detection of Cr6+ in the roots and shoots of Tagetes. The highest Cr6+ content was found in the roots and shoots of Tagetes sp. treated with Cr3+ 500 mg/l and Mn 10 mg/l at 0.059 and 0.035 g/l, respectively. The interaction of Cr3+ and Mn treatments significantly affected the growth of Tagetes plants. Cr3+ and Mn at high concentrations increased plant height, root length, root and shoot dry weight. The interaction of Cr3+ and Mn at high concentrations inhibited the increase in the number of leaves of Tagetes.
Efek Jamur Mikoriza Glomus intraradices terhadap Pertumbuhan Tagetes erecta L. pada Media Tanam Mengandung Kromium Jokosulistyo Wartanto; Sri Kasmiyati; Elizabeth Bety E.K
Bioedukasi: Jurnal Pendidikan Biologi Vol 13, No 1 (2020): Bioedukasi: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.329 KB) | DOI: 10.20961/bioedukasi-uns.v13i1.40399

Abstract

Tagetes erecta is an annual plant that is, in addition, to be used as an ornamental plant, it can also be used as an agent for phytoremediation. Glomus intraradices is one of the species of vesicular-arbuscular mycorrhizal (VAM) fungi which can be mutually symbiotic with various species of plants, including T. erecta.  This study was aimed to determine the effect of VAM fungi G. intraradices on the growth of T. erecta in growth media containing chromium. The study was carried out experimentally using a completely randomized design with treatments, namely Cr6+ concentrations of 0, 5 and 10 ppm in the form of K2CrO7, and the addition of VAM fungi G. intraradices in the growth media in the form of soil and mixture of soil: textile sludge (1: 1).  T. erecta was grown in four different growth media (soil, soil: textile sludge (1:1), soil+VAM, soil: textile sludge (1:1)+VAM) that was treated with Cr6+ and G. intraradices for 1.5 months. The growth of T. erecta was determined based on plant height, root length, root dry weight, shoot dry weight, and percentage of mycorrhizal infection. Data were analyzed with two-way Analysis of Variance (two-way ANOVA) and DMRT (Duncan's Multiple Range Test) with a test level of 5%. The results showed that the growth of T. erecta in media containing Cr6+ (5 and 10 ppm) decreased significantly compared to controls (without Cr6+ treatment).  The highest reduction of plant growth was found in media containing Cr6+ of 10 ppm. The addition of VAM fungi G. intraradices in soil media and soil: textile sludge that treated with Cr6+ was able to increase the growth of T. erecta. These results indicated that the VAM fungi G. intraradices were able to improve the ability of T. erecta to deal with chromium heavy metal stress. That results were also supported by the percentage of mycorrhizal infection in the roots of T. erecta grown in soil and soil: textile sludge (1:1) media with treatments of Cr6+ and VAM G. intraradices were higher than the control plants that was grown in soil and soil: textile sludge (1:1) media without treatments of Cr6+ and VAM G. intraradices.
Antibacterial Activity and Flavonoids Content of Artemisia cina Berg. ex Poljakov Ethyl Acetate Extracts Sri Kasmiyati; Elizabeth Betty Elok Kristiani; Maria Marina Herawati; Andreas Binar Aji Sukmana
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.27665

Abstract

The medicinal plant-derived bioactive compounds have a potential for many biological activities, including antimicrobial activity. Artemisia cina is a medicinal plant from the Compositae family with the potential of having antitumor, antifungal, and antibacterial activity. This study aimed to determine the antibacterial activity and the flavonoid content of A. Cina’s ethyl acetate extract. Plants samples were extracted by ethyl acetate maceration method. Antibacterial activity was tested against Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) by a disk diffusion method using 25, 50, and 100 mg/l extract concentrations. The flavonoid contents (quercetin and kaempferol) were measured using High-Performance Liquid Chromatography. The extracts of diploid and polyploid A. cina displayed some antibacterial activity, with the Gram-negative bacteria being more resistant than the Gram-positive counterpart. However, no significant difference was observed between the diploid and polyploid extracts. As for the flavonoid content, the highest quercetin content (0.5501 mg/ml) was found in the polyploid A. cina (J), while the highest kaempferol content (0.5818 mg/ml) was observed in the diploid A. cina (KJT). Although A. cina is widely grown in Indonesia, compared to other Artemisia species, A. cina has not been widely studied, especially its antibacterial  potential and in related to its flavonoid content and the use of ethyl acetate as the extraction solvent.  This study reveals the potential of A. cina as a natural antibacterial agent. 
Secondary Metabolites and Antioxidant Activity of Ethanolic Extract of Faloak (Sterculia quadrifida) Hory Iramaya Dillak; Elizabeth Betty Elok Kristiani; Sri Kasmiyati
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20736

Abstract

Faloak (Sterculia quadrifida) is a medicinal plant used by the people of East Nusa Tenggara to treat lumbago, liver dysfunction and to restore the stamina. The research aims were to determine the qualitative and quantitative content of flavonoids, phenols, and tannins, as well as to examine the antioxidant activity of roots, stem barks, leaves, fruits and seeds extracts of faloak plant. Each organ was extracted with ethanol 70% using the maceration method. The qualitative content of bioactive compounds was determined using the phytochemical screening method. The determination of bioactive compounds concentration was using spectrophotometric methods and antioxidant activity was using the DPPH method. The result of phytochemical screening showed that all of the extracts were exhibit phenols compounds, but the flavonoids and tannins were only found in roots, barks, leaves, and fruits extracts. The quantitative content of total flavonoids of roots, barks, leaves, fruits, and seeds was 48.09; 62.76; 12.56; 11.91 and 1.55 mg/g, while the phenols total content were 82.90; 45.37; 3.43; 29.50 and 2.89 mg/g. Tannins total content were 71.26; 59.64; 10.52; 13.18 and 14.12 mg/g samples respectively. The stem barks and roots extracts showed a very strong antioxidant activity, while leaves, fruits, and seeds extracts belong to the strong category.  The potential of faloak as an antioxidant has been widely studied, especially in the stem bark. Studies on the antioxidant activity of roots, leaves, fruits, and seeds can provide new information about the benefits of phaloac plants as a source of natural antioxidants.
The Combination of Phyllanthus niruri, Euphorbia hirta, and Loranthus sp. as a Source of Antioxidant Agents Elizabeth Betty Elok Kristiani; Sri Kasmiyati
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.30718

Abstract

Meniran (Phyllanthus niruri), patikan kebo (Euphorbia hirta), and benalu (Loranthus sp.) have often been used by people as medicinal plants. This research aimed to measure the levels of flavonoids, phenolics, and ascorbic acid compounds, as well as the Free Radical Scavenging (FRS) activity of ethyl acetate extract from the mixture of P. niruri, E. hirta, and Loranthus sp. The FRS activity was measured with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The level of compounds was measured by using the spectrophotometry method with specific reagents. The result of the FRS activity in ethyl acetate extract from the mixture of P. niruri, E. hirta, and Loranthus sp. varied depending on its dose. The measure of FRS in the P. niruri: E. hirta: Loranthus sp 0:0:1 (K-OOL) composition showed a strong result with a value of IC50 97.2 ± 2.1 ppm, while in the 0:0.5:0.5 (K-OEL) composition it was moderate with a value of IC50 147.6 ± 6.5 ppm. The other compositions showed weak and inactive results. The K-OOL composition had the highest flavonoid and phenolic content that were 298.8±0.00 mg QE/g extract and 141.5±2.85 mg GAE/g extract respectively. The composition with the highest ascorbic acid content (298.8±0.00 mg/g extract) was K-OEO composition. So far, research on medicinal plants is still limited to one type of plant. The combination of several types of plants in several formulations allows obtaining a composition that can produce maximum antioxidant capacity. Therefore, this research is expected to produce a combination formulation of various types of medicinal plants that have the K-OOL composition very strong antioxidant activity and can be used as herbal medicines.
Co-Authors ,, Djohan Agna Sulis Krave Agustien Sri Noerwahju Alberthnico, Ferdian Andreas Binar Aji Sukmana Anggara Mahardika Anna Helena Sabandar Araujo, Otilda Fatima Ariyanto, Prasetyo Eko Betty Elok, Elizabeth Cahyaningrum, Desti Christian Cantika Glodia Debora Kristiani Rahardjo Desti Christian Cahyaningrum Dewi Prabawaningrum Djohan Djohan Djohan Djohan Djurubasa, Dani Karel Marthin Egarani, Grahita Rezhi Elizabeth B.E Kristiani Elizabeth Betty Elok Kristiani Elizabeth Bety E.K Fanny Nurwahidah, Cantika Fathiya Ulfa Nurrasyida Fatima Araujo, Otilda Gabriella L. Mataradja Hapsari Sekar Widiantari, Olive Ie, Johanes Juan Yutama Putra Indrianasari, Putri Irfan Dwidja Priyambada, Irfan Dwidja Jokosulistyo Wartanto Juliana Sarioa Kachingwe, Baxter Hepburn Kapitarauw, Agnesya Giovani Putri Cendana Keliat, Natalia Rosa Kumala Dewi L. Hartanto Nugroho Lani, Rischa Karmila Margareta Prameswati Maria Marina Herawati Monica Mangudis, Vicharisti Nabila, Nabila Natalia Rosa Keliat Novellasari, Firdiana Penina Intansari Prasgi, Henokh Christian Pratama, Dimas Seno Bagus Priyayi, Desi Fajar Purwoko, Agus Putra Ie, Johanes Juan Yutama Putri, A C. Rahardja, P M. Ramagita, Marsahanda Astri Rimanti, Audri Septina Putri Rintawati Sandradewi, Rintawati Risya Pramana Situmorang Rully Adi Nugroho Sabandar, Anna Helena Santosa S, Santosa Satria Dharmawan Kustia Dewa Seleiman, Mahmoud F. Setya Magdalena, Ajeng Shinta, Damaris Clara Siahaya, Esty Vicariat Sucahyo Sucahyo Sucahyo Susanti Pudji Hastuti Susanti Puji Hastuti Syuhuud Arumbinang Wajdi Tambunan, Roslinda Kristin Trisianna, Jessica Ayu Trisianna Vincentia Irene Meitiniarti Vini Kurnia Ramadhani Wahyu Hari Kristiyanto Widyaningrum, Indy Yohanes Martono Yolanda Halim, Vivian