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Journal : Jurnal Veteriner

Pembekuan Semen Ayam Kampung Menggunakan Pengencer yang Diperkaya dengan Berbagai Konsentrasi dan Jenis Gula Khaeruddin Khaeruddin; Muhammad Erik Kurniawan
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to determine the type and concentration of sugar that was most suitable for use in semen extenders in freezing kampung rooster spermatozoa. Semen was collected from five roosters using massage method. Immediately after collection, the semen was evaluated macroscopically and microscopically. Semen was divided into twelve tubes. Each of them diluted with ringer lactate egg yolk added with various sugar such as glucose, trehalose, sucrose and lactose combined with sugar concentration of 20 mM, 50 mM and 80 mM. Liquid semen was packaged in a mini straw and was equilibrated, semen was frozen above the surface of the liquid nitrogen then it was stored in a container for 24 hours. Spermatozoa motility was evaluated after dilution, equilibration and thawing. The results showed that there were no significant differences in quality (P> 0.05) with the treatment of various types of sugars, but there were significant differences in motility (P <0.05) with differences in sugar concentrations. The best motility of spermatozoa was obtained at 50 mM and 80 mM sugar concentrations. Spermatozoa motility after thawing was 36.56-37.19% with a sugar concentration of 50 mM and 80 mM, whereas a sugar concentration of 20 mM was only resulting 34.06% of motility. The conclusion of this research is glucose, trehalose, sucrose and lactose can be added to the kampung rooster semen extender with a concentration of 50 mM for freezing.
Penentuan Konsentrasi Susu Skim Terbaik dalam Pengencer Semen Ayam Kampung Berbahan Dasar Ringer Laktat (DETERMINATION OF OPTIMUM SKIM MILK CONCENTRATION IN KAMPUNG CHICKEN’S SEMEN EXTENDER BASED ON LACTATED RINGERS) Khaeruddin Khaeruddin; Andi Nurlinda; Abdul Hakim Fattah; Andi Kurnia Armayanti
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to determine the best concentration of skim milk in maintaining the quality of Kampung chicken’s spermatozoa during storage at 5 oC. Semen was collected from five roosters which used massage method. Furthermore, semen was divided into five tubes as treatment (no extenders, extenders containing 0%, 6%, 9% and 12% skim milk concentrations), the liquid semen was stored at 5 oC. Observations of motility, viability and abnormalities of spermatozoa were carried out at 0, 12, 24, 36, dan 48 hours. The results showed that there was a very significant difference (P <0.01) among the treatment of motility, viability and abnormalities of Kampung chicken spermatozoa after dilution during storage. Storage of spermatozoa in 6% skim milk concentrate for 48 hours was resulted in motility of 49,00±1,30%, viability of 52,13±1,53% and abnormalities of 20,47±0,47%. The use of lactated ringer extenders with a concentration of 6% skim milk can generally maintain sperm quality which was better than other treatments during storage.