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All Journal Indonesian Journal of Educational Studies Indonesian Journal of Biotechnology JURNAL BIOLOGI INDONESIA JOIV : International Journal on Informatics Visualization Jurnal IPA Terpadu Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi Jurnal Pengabdian Magister Pendidikan IPA KNOWLEDGE: Jurnal Inovasi Hasil Penelitian dan Pengembangan Seminar Nasional Pengabdian Kepada Masyarakat Indonesian Journal of Fundamental Sciences SMART: Jurnal Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) JKM: Jurnal Kemitraan Masyarakat Jurnal Bioteknologi & Biosains Indonesia
Nurhayani H. Muhiddin, Nurhayani H.
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ISOLATION OF ANTIBIOTIC PRODUCING MICROORGANISMS FROM CASSAVA ROOT TAPAI BY TRADITIONAL FERMENTED Muhiddin, Nurhayani H.; Hidayah, Nurul; Herawati, Netty; Ramlawati; Sukmadewi. M, Fitrah; Darmayani , Dwi; Marnia; Idris, Nur Afni Yunita
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.11634

Abstract

Antibiotic resistance among pathogenic microorganisms has become a growing global health concern, necessitating the search for new natural sources of antimicrobial compounds. Cassava tapai, a traditional Indonesian fermented food, contains molds, yeasts, and bacteria with potential antibiotic-producing abilities. This study aimed to isolate and characterize antibiotic-producing microorganisms from cassava tapai fermented with local Sulawesi ‘Ragi’. Isolation, colony morphology characterization, and antibiotic activity tests were conducted using the Kirby–Bauer method against Escherichia coli ATCC 35218 and Staphylococcus aureus ATCC 25923. Inhibition zones were measured with a digital caliper, and data were analyzed using one-way ANOVA followed by Tukey’s HSD test or Kruskal–Wallis followed by the Mann–Whitney U test (p < 0.05). Three isolates Bb1, Bb2, and Wk3 exhibited significant antibiotic activity, with Bb1 showing stronger inhibition against E. Coli ATCC 35218. These results highlight confirms the potential of cassava tapai as a promising source of antibiotic-producing microorganisms for future antimicrobial discovery.
Co-Authors - Wulandari, - Ahmad, Sitti Wirdhana Aisyah, Dyah Maulidina Akhmad Syakur Andi Afrinaramadhani Hatta Arie Arma Arsyad Arif, Rifda Nur Hikmahwati Arif, Rifda Nur Hikmawati Damayanti, Putri Darmayani , Dwi Fakhirah, Nurul Fandi Ahmad Hasan, Nur Fadhilah Hasanuddin Hasanuddin Idris, Nur Afni Yunita LANGKAH SEMBIRING Marnia Muhammad Aqil Rusli, Muhammad Aqil Muhammad Farid Netty Herawati Nur Arfa Yanti Nur Indah Sari Nurfitra Yanto Nurhalisa, Siti NURUL HIDAYAH Rahmat, Irwandi Ramlawati Ramlawati Ramlawati Sahrani, Sahrani Sahribulan, Sahribulan Saleh, Andi Rahmat Saleh, Salsabila Yusuf Salsabila, Putri Samputri, Salma Sebastian Margino Selvi Marcellia Sembang, Timiati Shirafin Sitti Saenab, Sitti Sukmadewi M, Fitrah Sukmadewi. M, Fitrah Taufiq, A. Husnawati U, Sahrani Yunus, Sitti Rahma Zahrah, St.