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The Potential Antibacterial Effect of Papaya Leaf Extract (Carica papaya L) and Miana Leaf Extract (Coleus scutellarioides L) as Adjuvant Therapy for Rifampicin-Resistant Tuberculosis Wawan Sofwan Zaini; Nining Kurniati; Khayan Khayan; Bagus Muhammad Ihsan; Widyana Lakshmi Puspita; Muhammad Ifham Hanif
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 1 (2023): May
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i1.2044

Abstract

The adhesion of Rifampicin-resistant TB to neutrophils plays an essential role in colonization. Several active compounds in papaya leaf and Miana leaf (Coleus scutellarioides L) are believed to regulate or prevent the formation of bacterial colonies. The purpose of this study was to determine the anti-bacterial effectiveness of extracts of papaya leaf (Carica Papaya L.) and Miana leaf (Coleus scutellarioides L) against bacterial isolates of Rifampicin-Resistant TB strain (RR). This research method is a in vitro laboratory experiment, and extracts of papaya leaf and Miana leaf (50, 25, 12,5%) were tested as anti-bacterial using the M-TB susceptibility test using the Proportion Method. The results showed the anti-bacterial ability of papaya leaf extract against bacterial isolates of the MTBC-Resistant Rifampicin strain at a concentration of 50% with a resistance percentage value of 0% so that it was included in the Sensitive category, but at a concentration of 25% the resistance percentage value was 42.86% % and a concentration of 12.5%, the percentage value of resistance is 42.86% so that it is included in the category of resistance (Resistant > 1% and Sensitive < 1%). The anti-bacterial ability of miana leaf extract against bacterial isolates of the MTBC-Resistant Rifampicin strain at a concentration of 50% with a resistance percentage value of 5.33%, at a concentration of 25%, with a resistance percentage value of 17.14%, and at a concentration of 12.5%, with a resistance percentage value of 100%, so all are included in the resistant category. The Conclusion 50% papaya leaf extract inhibits the formation of Rifampicin-resistant MTBC-resistant bacterial colonies, allowing its usage as a substitute ingredient in Rifampicin-resistant MTBC-resistant medications.
Efektivitas Pasir dan Karbon Aktif dalam Menurunkan Kekeruhan dan Timbal Pada Air Hujan Khayan Khayan; Taufik Anwar
Jurnal Vokasi Kesehatan Vol 2, No 2 (2016): Juli 2016
Publisher : Poltekkes Kemenkes Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.613 KB) | DOI: 10.30602/jvk.v2i2.70

Abstract

Abstract: The Effectiveness of Sand And Activated Carbon For Lowering Turbidity Tubes And Pablum On Roof Rain Water. This research was aimed to make filtration tube by using sand, shells and coconut shell activated carbon, also analyze its effectiveness in reducing Pb and turbidity of rain water. This experimental research uses pre and post-test design with control. The results show that level of lead (Pb) pre-treatment about 131.7 μg/l and 20 NTU of turbidity. Level of Pb post-treatment was 0.71 ug / l and 5.66 NTU of turbidity with 99.4% effectiveness in decreasing lead (Pb) and 72% effectiveness in decreasing turbidity. Results of statistical analysis showed that there were a differences levels of Pb and turbidity before and after treatment (p = 0.00)Abstrak: Efektivitas Pasir dan Karbon Aktif Dalam Menurunkan Kekeruhan dan Timbal Pada Air Hujan.Tujuan penelitian yaitu untuk menganalisis efektifitas pasir dan karbon aktif dalam menurunkan timbal dan kekeruhan pada air hujan. Jenis penelitian adalah eksperimen dengan rancangan pre and post test with control design. Hasil penelitian sebelum perlakuan menunjukkan kadar timbal (Pb) 131,7 μg/l dan kekeruhan sebesar 20 NTU. Setelah perlakuan kadar Pb sebesar 0,71 μg/l dan kekeruhan 5,66 NTU dengan efektivitas penurunan timbal (Pb) 99,4% dan kekeruhan, 72%. Hasil analisis statistik menunjukkan adanya perbedaan kadar Pb dan kekeruhan sebelum dan sesudah dilakukan pengolahan (p= 0,00).
HUBUNGAN PENGATURAN WAKTU PENAMPUNGAN AIR HUJAN DENGAN PENURUNAN KERACUNAN Pb PADA MASYARAKAT DI KOTA PONTIANAK (Relation Between The Time Control of Rain Water Collection with The Decrease of Pb Intoxication for Community at Pontianak, Kalimantan) Khayan Khayan; Sudarmadji Sudarmadji; Adi Heru Sutomo
Jurnal Manusia dan Lingkungan Vol 10, No 1 (2003): Maret
Publisher : Pusat Studi Lingkungan Hidup Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jml.18601

Abstract

ABSTRAK Penelitian ini bertujuan (1) mencermati hubungan antara pengaturan waktu penampungan air hujan dengan penurunan keracunan Pb, (2) menemukan perbedaan keracunan Pb antara masyarakat yang meminum air hujan dari air yang ditampung melalui atap seng dan bukan atap seng, (3) memahami korelasi antara pekerjaan perilaku merokok, jenis sumber air minum dan tempat pengumpulannya, dan jarak antara rumah dengan derajad keracunan Pb, dau (4) menemukan hubungan antara keracunan Pb dan gejala subyektif antara lain sakit kepala, kelelahan, nyeri perut diare, muntah-muntah dan gangguan tidur. Studi in menggunakan pendekatan quasi experiment. Subyek penelitian ini adalah masyarakat yang menggunakan air hujan sebagai air minum. Sampling dilaksanakan menggunakan cluster random sampling. Pengumpulan data dilakukan menggunakan teknik wawancara dan quesioner, pencermatan konsentrasi Pb digunakan metode AAS di laboratorium. Analisis dilakukan secara deskriptif dan analitis menggunakan uji korelasi dan t-test. Hasil penelitian menunjukkan bahwa (1) pengaturan waktu penampungan sekitar 20 menit dapat menurunkan tingkat keracunan Pb, (2) tidak ditemukan perbedaan antara tingkat keracunan Pb bagi mereka yang meminum air dari air yang ditampung melalui atap seng dan bukan seng, (3) tidak ditemukan hubungan antar factor pekerjaan, perilaku merokok, jenis sumber air dan tempat penampungan dan jarak rumah terhadap tingkat keracunan Pb, dan (4) tidak ditemukan hubungan antara tingkat keracunan Pb dengan gejala subyektif masyarakat antara lain sakit kepala, kelelahan, nyeri perut diare, muntah-muntah dan gangguan tidur. ABSTRACTConcentration of Pb in rain water, although its very low but it is dangerous and able to damage public health. In the body, Pb exposure will be absorpted and distributed by blood and a part of Pb content will be accumulated in the tissue. To decrease the Pb concentration of rain water, one of its to control time of rain water collection, that used to supply drinking water a daily for community in Pontianak city. the aims of this research are: (1) to examine the relation between the time control of collection rain water with the decrease of Pb intoxication, (2) to find difference of Pb intoxication between the community who drank rain water from zinc roof and non-zinc roof, (3) to understand correlation between jobs, smoking behavior, kinds of drink water source and it’s collection places, and distance of house with degree of Pb intoxication and, (4) to find the relation between Pb intoxication with the subjectivity symptom, which are headache, fatigue, abdomine pain, diarrhea, vomit and sleep disturbance. The type of study used was a quasi experiment. The subject of research were a group community and rain water, that supplied for drinking water. The sampling was carried out cluster random sampling. Data collection was used interview with questionnaire and examine of Pb concentration with AAS method at laboratorium. data collected will be analized by descriptive and analytically use correlation and t test. The result of this research showed that: (1) time control of rain water collection about 20 degree of Pb intoxication between community, who drank rain water from zinc roof and non-zinc roof, (3) it has not found relation between factors of jobs, smoking behavior, kinds of drink water source and its collection places and distance house with the degree of Pb intoxication, and (4) it has not found the relation of the degree of Pb intoxication with the community health disorder (subjectivity symptom), which were headache, fatigue, abdomine pain, diarrhea, vomit, and sleep disturbance.
Utilization of Ginger Powder (Zingiber officinale) as Larvicide for Aedes Aegypti Alkausyari Aziz; Khayan; Slamet Wardoyo
Gema Lingkungan Kesehatan Vol. 22 No. 1 (2024): Gema Lingkungan Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/gelinkes.v22i1.117

Abstract

Aedes aegypti mosquitoes are known vectors for Dengue Fever (DF). This disease is caused by the Dengue virus, necessitating control measures that do not harm public health. One such measure is the use of ginger powder (Zingiber officinale) as a larvicide against Aedes aegypti larvae, the vector of DF. This experimental study employed a Completely Randomized Design with statistical analysis using the F-test. Four treatment concentrations and one control were tested: 5 grams, 10 grams, 15 grams, and 20 grams, with three repetitions for each treatment, observed over a 4-hour period. The study aimed to determine the effect of ginger powder (Zingiber officinale) as a larvicide on Aedes aegypti larvae. The average larval mortality at a concentration of 5 grams was 4.3 larvae, 7.6 larvae at 10 grams, and 10 larvae at both 15 grams and 20 grams. The F-test results showed that at the 1% level, Fht > Ftb. Duncan's Multiple Range Test indicated that the effective concentration was 15 grams, at which all Aedes aegypti larvae died within 4 hours. The utilization of ginger powder (Zingiber officinale) as a larvicide, with varying amounts of powder, resulted in different larval mortality rates. The higher the concentration of ginger powder (Zingiber officinale), the higher the larval mortality rate of Aedes aegypti.
Optimization of Drying Temperature for Bintaro Fruit Seeds (Cerbera manghas) as a Bioinsecticide for Aedes aegypti Using a Tray Dryer Fitria Gusfa; Alkausyari Aziz; Khayan; Taufik Anwar
Gema Lingkungan Kesehatan Vol. 22 No. 2 (2024): Gema Lingkungan Kesehatan
Publisher : Poltekkes Kemenkes Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36568/gelinkes.v22i2.172

Abstract

As a tropical country, Indonesia has various plants that can be utilized for human needs. One such plant is Bintaro (Cerbera manghas). Bintaro contains secondary metabolites that can be used as a bioinsecticide. These secondary metabolite compounds can degrade if dried at high temperatures. This study focused on optimizing the drying temperature of Bintaro fruit seeds (Cerbera manghas) using a tray dryer to produce bioinsecticide. The research employed a Completely Randomized Design (CRD) with four different drying temperature treatments: 40°C, 50°C, 60°C, and 70°C. The ethanol extracts from Bintaro seeds produced at each temperature treatment were tested for their effectiveness as a bio-insecticide against Aedes aegypti mosquitoes. The results indicated that the optimal drying temperature for Bintaro seeds, which provided the best bioinsecticide effectiveness, was 50°C.
Health Education Media to Increase Vegetable and Fruit Consumption in School-Aged Children Widyana Lakshmi Puspita; Khayan; Suaebah; Bagus Muhammad Ihsan; Muhammad Ifham Hanif
Jurnal Promkes: The Indonesian Journal of Health Promotion and Health Education Vol. 12 No. 2 (2024): Jurnal Promkes: The Indonesian Journal of Health Promotion and Health Educatio
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jpk.V12.I2.2024.160-167

Abstract

Background: The lack of regulatory substances from fruits and vegetables is related to individual factors. Aims: The purpose of this study is to determine the effect of using educational media, lift-the-flap books, and leaflets on increasing knowledge, attitudes, parental support, and consumption of vegetables and fruit in elementary school children. Methods: This type of research was a quasi-experiment with a pre-and post-design approach to see the causal relationship between before and after counseling intervention with lift-the-flap book and leaflet media on increasing knowledge, attitudes, parental support, and consumption of vegetables and fruits in school-age children. Measuring knowledge scores, attitudes, and parental support was done using a Likert scale, and vegetable and fruit consumption using a 2x24-hour recall. Statistical analysis was conducted using the Test correlation test with a 95% confidence level (α = 5%). Results: Based on the research, it can be seen that using the lift-the-flap books and leaflet media can increase knowledge, attitudes, parental support, and consumption of vegetables and fruit compared to before using the media in elementary school children Pontianak. Conclusion: Lift-the-flap books are more effective in increasing knowledge, attitudes, parental support, and consumption of vegetables and fruits than leaflets.
PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION Ihsan, Bagus Muhammad; Sucipto, Cecep Dani; Khayan, Khayan
Journal of Vocational Health Studies Vol. 5 No. 1 (2021): July 2021 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V5.I1.2021.26-30

Abstract

Background: Filariasis patients can be a source of transmission if their blood still contains microfilariae. One of the Polymerase Chain Reaction (PCR) methods used is High Resolution Melting (HRM), using primary specificity testing. Purpose: To test the specificity of SLX primer. The samples used for this test were isolates of Salmonella., Klebsiella, Pseudomonas, negative and positive controls for Brugia malayi and Wuchereria bancrofti. Method: The design in this study is a quasi-experiment by testing the specificity of SLX primer using HRMbased real-time PCR based on the Cycle Threshold (CT) value observed through the amplification curve. Result: The real-time PCR results showed that no CT was released in the bacterial samples, and there was a CT value in the positive control. The results of this study indicate that specific SLX primer can be used in identifying microfilariae. Conclusion: SLX primer have a reasonable specificity because they cannot detect the existence of microorganisms in the samples other than microfilariae.
IDENTIFICATION OF MICROFILARIAE USING CONVENTIONAL POLYMERASE CHAIN REACTION AND QPCR-HRM Ihsan, Bagus Muhammad; Lakshmi Puspita, Widyana; Triana, Linda; Wahdaniah; Khayan; Dani Sucipto, Cecep
Journal of Vocational Health Studies Vol. 8 No. 1 (2024): July 2024 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V8.I1.2024.42-47

Abstract

Background: The presence of filarial worms in the lymph nodes can result in acute symptoms, such as inflammation of the lymph nodes and ducts, particularly in the groin region. As part of the life cycle of filariasis, symptomatic or asymptomatic patients with microfilariae in their blood can transmit the disease via mosquito bites. The inspection of microfilariae that is currently being developed uses Polymerase Chain Reaction (PCR) to carry out a unique DNA search technique. Purpose: Identify the type of microfilaria present in filariasis patients using Quantitative PCR High- Resolution Melting (qPCR-HRM) and conventional PCR techniques. Method: This study involved the examination of 19 samples using the qPCR-HRM method. Subsequently, the results that were considered positive for microfilaria underwent further testing using conventional PCR. Result: The results of the examination using these two methods revealed the presence of Brugia malayi and Wuchereria bancrofti microfilariae with peak melting temperatures ranging from 78.2 – 78.7 °C and 80.8 – 81.2 °C, and fragment sizes of 199 bp and 227 bp, respectively. Conclusion: Based on the results of the identification from these two methods, it is evident that microfilariae of Brugia malayi and Wuchereria bancrofti can be detected using both conventional and qPCR-HRM methods.
Aplikasi teknologi tepat guna pengolahan air sebagai sumber air bersih masyarakat Khayan; Ihsan, Bagus Muhammad; Sucipto, Cecep Dani; Puspita, Widyana Lakshmi
Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Vol. 4 No. 2 (2023)
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jp2m.v4i2.20460

Abstract

Gangguan kesehatan di Indonesia yang berhubungan dengan penyakit yang ditularkan melalui air (Water Borne Disease) masih tergolong tinggi, seperti Diare dan hepatitis. Timbulnya penyakit menular terkait Water Borne Disease tersebut, diantaranya karena tidak terpenuhinya kuantitas dan kualitas air baku sebagai sumber air minum yang memenuhi syarat kesehatan. Metode pengabdian masyarakat ini dilakukan beberapa metode, yaitu pendekatan masyarakat, penyuluhan dan pembuatan sarana Pengolahan Air Permukaan dengan Teknologi Tepat Guna (TTG) kombinasi, yaitu dengan Aerasi, Filtrasi Pasir dan Absorpsi Kabon Aktif untuk Menurunkan kandungan Fe, Kekeruhan, Warna dan pH Air permukaan sebagai sumber air minum masyarakat di Desa Pranan Kota Serang. Hasil dan kesimpulan dari pengabdian pada masyarakat ini adalah Terdapat peningkatan pengetahuan masyarakat tentang TTG Filtrasi Air Bersih , Terciptanya revitalisasi sumber air bersih, transfer teknologi serta dampak sosial bagi masyarakat yaitu perubahan pola kegiatan mandi dan mencuci yang sebelumnya dilakukan di kali pembuangan air sawah setelah pelaksanaan pengabdian kegiatan tersebut beralih ke rumah masing-masing dan Metode penjernihan air secara filtrasi terbukti dapat meningkatkan kualitas air sumur bor ,sehingga air berada pada ambang batas layak digunakan untuk kehidupan sehari-hari. Dengan ini masyarakat setempat tidak lagi mengambil air dari sungai pembuangan air sawah.
Identification of Pathogenic Bacteria in Tofu Water and Sugarcane Water in Pontianak City Hermantika, David Deovia Triputra; Ihsan, Bagus Muhammad; Kamilla, Laila; Khayan, Khayan; Hanif, Muhammad Ifham
Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 4 (2024): February
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v17i4.3336

Abstract

Tofu water and sugar cane juice are trendy drinks, especially in Pontianak City. Pathogenic bacterial contamination in tofu water and sugar cane juice can be a severe threat if the immune system is weak. This research aims to identify pathogenic bacteria in Pontianak City tofu water and sugar cane water. The methods used are culture methods and biochemical tests. The research samples were tofu water and sugar cane juice sold in the community. The sample was selected using a purposive sampling technique. In this study, the bacteria found in the identification of bacteria in tofu water and sugar cane water were Klebsiella pneumoniae, Shigella dysenteriae, Staphylococcus aureus, Enterobacter aerogenes, Salmonella typhi, and Escherichia coli. Bacteria dominate, while in sugarcane juice, it is dominated by Escherichia coli, followed by Klebsiella pneumoniae. The research results can also be the basis for better policies and preventive measures related to the safety of the quality of tofu water and sugar cane juice in Pontianak City. Therefore, this research can be relevant to environmental protection and public health in Pontianak.