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All Journal VALENSI International Journal of Renewable Energy Development Jurnal Keramik dan Gelas Indonesia Al-Kimia Chimica et Natura Acta ALCHEMY Jurnal Penelitian Kimia Indonesian Journal of Chemistry PENDIPA Journal of Science Education Molekul: Jurnal Ilmiah Kimia Jurnal Keramik dan Gelas Indonesia Journal of Advanced Technology and Multidiscipline (JATM)
Yeni Wahyuni Hartati
Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Electrical & Electronics Engineering Energy Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics

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Study of The Interaction Carbondioxide Gas with Hydroxyapatite Using Density Functional Theory Atiek Rostika Noviyanti; Azhari Yusuf; Yeni Wahyuni Hartati; Budi Adiperdana
Jurnal Keramik dan Gelas Indonesia Vol 30, No 2 (2021): Jurnal Keramik dan Gelas Indonesia
Publisher : Balai Besar Keramik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32537/jkgi.v30i2.7175

Abstract

Hydroxyapatite (HA) is a phosphate mineral with Ca10(PO4)6(OH)2 chemical formula. The hydroxyl group in HA allows ion to exchange, so this material may be applied as a CO2 gas sensor. The interaction between HA with CO2 gas is difficult to observe directly, and ab initio modeling is necessarily to investigate the interactions between HA with CO2 gas. In this study, a simulation was conducted using the density functional theory method to determine the interaction between CO2 gas on the surface of HA. The simulation results indicate that a large HA surface is required for optimal CO2 gas adsorption. Pore diameter size needs to be larger than 2.5 Å to prevent binding on two sides of the surface pores. 
Application of Gold Silica Nanocomposites in Electrochemical Biosensors: A Review Salma Nur Zakiyyah; Diana Rakhmawaty Eddy; M. Lutfi Firdaus; Yeni Wahyuni Hartati
PendIPA Journal of Science Education Vol 5, No 2 (2021): MARCH - JUNE
Publisher : University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/pendipa.5.2.122-132

Abstract

Gold silica nanocomposite-based biosensors are performing well in sensor technology for biosensor development. Due to this biosensor has good selectivity, excellent conductivity, large surface area, efficient enhancement of electron transfer between enzymes and electrodes and good biocompatibility. Therefore, gold silica nanocomposite can be an ideal matrix for immobilization of biomolecules. This review describes the method of synthesizing gold silica nanocomposites and their characterization, interaction with biomolecules and application of gold silica nanocomposites in electrochemical biosensors.
Review: Sintesis Dan Karakterisasi Nanopartikel Emas (AuNP) Serta Konjugasi AuNP Dengan DNA Dalam Aplikasi Biosensor Elektrokimia Egista Istioka Fazrin; Annisa Ilma Naviardianti; Santhy Wyantuti; Shabarni Gaffar; Yeni Wahyuni Hartati
PendIPA Journal of Science Education Vol 4, No 2 (2020): MARCH - JUNE
Publisher : University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (726.164 KB) | DOI: 10.33369/pendipa.4.2.21-39

Abstract

The use of gold nanoparticles (AuNP) is an appropriate one in the development of biosensors because it has a unique, strong adsorption, high biocompatibility, and large surface area. AuNPs can be synthesized by various methods with the same synthesis principle by reducing Au3+ to Au. This review explains the various methods of AuNP synthesis and their characteristics, the interaction of AuNP with biomolecules including DNA, and the application of AuNP-DNA bioconjugates in biosensors. Several applications of AuNP-DNA bioconjugates as electrochemical biosensors in the past two years are proposed in this review.
An Electrochemical Immunosensor for the Detection of B-Type Natriuretic Peptide (BNP) Based on Sandwich ELISA Using Screen Printed Carbon Electrodes Yeni Wahyuni Hartati; Muhammad Hilman Daniswara; Ratna Nurmalasari; Shabarni Gaffar; Toto Subroto
Molekul Vol 13, No 1 (2018)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.537 KB) | DOI: 10.20884/1.jm.2018.13.1.379

Abstract

B-type natriuretic peptide (BNP) is a 32-amino acid polypeptide, a cardiac neuro hormone that specifically secreted from heart ventricle as a response towards the increase of volume and pressure in the heart. The determination of BNP concentration in patients blood is one of the method used to diagnose heart failure. An electrochemical immunosensor modified with the streptavidin/biotin system on screen printed carbon electrodes for the detection of the BNP antigen was developed in this study. Monoclonal anti-BNP capture antibody was immobilized on streptavidin-modified SPCEs to give a well oriented of antibody. Furthermore, a biotinylated anti-BNP that conjugated with horseradish peroxidase (HRP) was used as secondary antibody. The electrochemical signal produced by redox activity of substrate 3,3,5,5'-tetramethybezidine dihydrochloride (TMB/H2O2) was measured by differential pulse voltammetry. The BNP immunosensor showed a linear response between 1.0×10-2 and 1.0×102 ng/mL, and the limit of detection was 3.3 ng/mL. BNP immunosensor is a promising technology for the rapid and convenient detection of heart failure.
Biosensor DNA Elektrokimia untuk Deteksi Makanan Mengandung Babi (Sus scrofa) Menggunakan Screen Printed Carbon Electrode Termodifikasi Emas Shabarni Gaffar; Annisa Ilma Naviardianti; Santhy Wyantuti; Yeni Wahyuni Hartati
ALCHEMY Jurnal Penelitian Kimia Vol 18, No 1 (2022): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.18.1.50170.37-47

Abstract

Metode deteksi berbasis deoxyribonucleic acid (DNA) merupakan metode yang paling akurat, spesifik, dan sensitif untuk mengidentifikasi adanya campuran komponen babi dalam produk pangan. Penelitian ini bertujuan untuk mengembangkan biosensor DNA secara elektrokimia menggunakan Screen Printed Carbon Elektrode termodifikasi emas (SPCE-Au) untuk mendeteksi DNA babi dalam makanan olahan. Permukaan SPCE dimodifikasi dengan emas menggunakan metode adsorbsi pasif, kemudian dikarakterisasi dengan Scanning Electron Microscopy (SEM) dan Differential Pulse Voltammetry (DPV). DNA probe yang spesifik terhadap DNA mitokondria babi diimobilisasi ke permukaan SPCE-Au melalui perantara gugus tiol. Proses hibridisasi DNA probe-DNA komplemen dikarakterisasi menggunakan DPV berdasarkan sinyal oksidasi guanin. Kondisi percobaan dioptimasi menggunakan desain eksperimen Box-Behnken, yaitu konsentrasi DNA probe (0,5; 1,0; 1,5 µg/mL), waktu imobilisasi DNA probe (10, 20, 30 menit), dan waktu hibridisasi DNA probe-DNA komplemen (5, 10, 15 menit). Kondisi optimum digunakan untuk menentukan respons arus oksidasi guanin menggunakan DPV terhadap variasi konsentrasi DNA komplemen. Selanjutnya, biosensor DNA diaplikasikan terhadap sampel bakso yang mengandung campuran daging babi, ayam, dan sapi. Hasil penelitian menunjukkan modifikasi SPCE dengan Au menghasilkan peningkatan arus yang diuji menggunakan sistem redoks K3[Fe(CN)6] secara DPV. Kondisi optimum percobaan adalah: konsentrasi DNA probe 1 µg/mL, waktu imobilisasi DNA probe 20 menit, dan waktu hibridisasi DNA probe-DNA komplemen 10 menit. Biosensor DNA ini memiliki batas deteksi 0,31 µg/mL, batas kuantifikasi 1,06 µg/mL dan recovery 99,2% untuk rentang konsentrasi 0,1 - 2 µg/mL. Deteksi sampel bakso menggunakan biosensor DNA menunjukkan peningkatan respons arus yang signifikan pada sampel yang mengandung 100% daging babi (3,417 µA) dan masih dapat mendeteksi adanya kandungan daging babi sampai 1%. Metoda biosensor DNA babi menggunakan SPCE-Au tanpa indikator dan biokonjugat yang dikembangkan lebih sederhana dan cepat dan mudah untuk diaplikasikan ke sampel nyata. Electrochemical DNA Biosensor for Detection of Pork (Sus scrofa) Using Gold Modified Screen Printed Carbon Electrode. The DNA-based detection method is the most accurate, specific, and sensitive method for identifying the presence of a mixture of pork components in food products. This study aims to develop an electrochemical DNA biosensor using a gold-modified Screen Printed Carbon Electrode (SPCE-Au) to detect pork DNA in processed food. The surface of the SPCE was modified with gold using a passive adsorption method, then characterized by scanning electron microscopy (SEM) and differential pulse voltammetry (DPV). The probe DNA specific to porcine mitochondrial DNA was immobilized to SPCE-Au surface via an intermediate thiol group. The hybridization of probe DNA-complement DNA was characterized using DPV based on the guanine oxidation signal. The experimental conditions were optimized using the Box-Behnken experimental design by applying probe DNA concentration (0.5; 1.0; 1.5 µg/mL), the immobilization of the probe DNA (10, 20, 30 minutes), and the hybridization of the probe DNA-complement DNA (5, 10, 15 minutes). The optimum conditions were used to determine the DPV current response vs. complementary DNA concentrations. Furthermore, the DNA biosensors were applied to meatball samples containing a mixture of pork, chicken, and beef. The results showed that modification of SPCE with Au produced an increase in current responses tested using K3[Fe(CN)6] redox system by DPV. The optimum conditions of the experiment were: probe DNA concentration was 1 µg/mL, time to immobilize probe DNA was 20 minutes, and time to hybridize probe DNA-complement DNA was 10 minutes. The limit of detection, limit of quantification and percent recovery of DNA biosensor was 0.31 µg/mL, 1.06 µg/mL and 99.2%, respectively. Detection of meatball samples by DNA biosensor showed a significant increase of current response for sample that contains 100% swine DNA (3.417 µA). The biosensor is still able to detect the pork content until 1%. The pork DNA biosensor using SPCE-Au without indicator and bioconjugate developed in this study is simpler and applicable to real samples. 
Penerapan Desain Eksperimen Plackett-Burman dan Box-Behnken pada Analisis Voltametri Pulsa Diferensial untuk Penentuan Kadar Senyawa Kompleks Gd-DTPA Santhy Wyantuti; Ravenna Aristantia; Yeni Wahyuni Hartati; Husein H Bahti
ALCHEMY Jurnal Penelitian Kimia Vol 16, No 1 (2020): March
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.761 KB) | DOI: 10.20961/alchemy.16.1.35166.140-151

Abstract

Gadolinium merupakan salah satu golongan unsur tanah jarang yang menarik karena memiliki sifat paramagnetik yang tinggi. Pengkhelatan gadolinium dengan ligan dietilentriaminpentaasetat (DTPA) menghasilkan senyawa yang berguna dalam bidang kesehatan. Tujuan dari penelitian ini adalah menentukan kandungan unsur tanah jarang gadolinium sebagai kompleks dengan ligan DTPA secara voltametri pulsa diferensial dengan penerapan desain eksperimen Plackett-Burman dan Box-Behnken. Desain eksperimen Plackett-Burman digunakan untuk menyeleksi parameter yang memiliki relevansi positif terhadap respon arus. Parameter yang terpilih kemudian dioptimasi melalui desain eksperimen Box-Behnken sehingga diperoleh kondisi optimum tiap parameter. Parameter yang terseleksi meliputi konsentrasi ligan DTPA, waktu deposisi dan waktu kesetimbangan dengan nilai tiap parameter pada kondisi optimum masing-masing yaitu 150, 13 mgL-1, 60 detik dan 30 detik.  Berdasarkan hasil pengukuran pada kondisi optimum didapat nilai presisi dan akurasi untuk kompleks Gd-DTPA yaitu 99,79% dan 93,42% dengan nilai limit deteksi dan kuantisasi untuk kompleks Gd-DTPA yaitu 3,97 mg/L dan 10,71 mg/L. The Application of Experimental Designs of Plackett-Burman and Box-Behnken in Differential Pulse Voltammetry Analysis for Gd-DTPA Complex Detection. Gadolinium is one of rare earth elements that is interesting because it has high paramagnetic properties. The gadolinium chelating with diethylentriaminpentaacetic acid (DTPA) ligands produces useful compounds in the health field. The purpose of this experiment is to determine amount of gadolinium as complex with DTPA on differential pulse voltammetry by applying Plackett-Burman and Box-Behnken experimental design. The method employed experimental design of Plackett-Burman to select factors, which have positive relevance to response. The selected factors are optimized based on experimental design of Box-Behnken to obtain the optimum condition each factors. The selected factors are ligand concentration, deposition time and equilibrium time with optimum value of 150 mgL-1, 60 s and 30 s, respectively. Based on the result of measurement at optimum condition, the precision and accuracy value for Gd-DTPA complex is 99.79% and 93.42% with detection and quantization limit value for Gd-DTPA complex is 3.97 mg/L and 10.71 mg/L.
Pengaruh Konsentrasi Natrium Silikat Terhadap Laju Korosi Paduan Aluminium dalam Lingkungan Natrium Klorida 3,5% Rukiah Rukiah; Diding Mandala Putra; Solihudin Solihudin; Yeni Wahyuni Hartati; Atiek Rostika Noviyanti
ALCHEMY Jurnal Penelitian Kimia Vol 16, No 2 (2020): September
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.16.2.40927.218-226

Abstract

Korosi merupakan proses penurunan kualitas logam akibat reaksi logam dengan lingkungannya. Air laut merupakan salah satu sistem yang korosif karena tingginya kadar ion klorida. Ion silikat dengan konsentrasi optimum mampu memperlambat laju korosi pada logam, seperti aluminium. Penelitian ini menguji pengaruh ion silikat dalam larutan natrium klorida 3,5% terhadap laju korosi aluminium yang diuji dengan metode potensiostat dan dihitung dengan Ekstrapolasi Tafel. Pengaruh konsentrasi ion silikat terhadap laju korosi diamati pada variasi konsentrasinya yaitu 0, 25, 50, 75, dan 100 mM. Konsentrasi optimum natrium silikat untuk menurunkan laju korosi adalah 25 mM,  laju korosi turun dari 0,0118 menjadi 0,0084 mm/tahun. Sebaliknya, penambahan konsentrasi natrium silikat hingga 100 mM,  dapat meningkatkan laju korosi menjadi 0,101 mm/tahun.The Effect of Sodium Silicate Concentration on the Corrosion Rate of Aluminum Alloy in Sodium Chloride 3.5%. Corrosion is a process of decreasing the quality of metals due to the reaction of metals with their environment. Seawater is one of the corrosive systems because of the high levels of chloride ions. Silicate ions with optimum concentrations can slow the rate of corrosion in metals, such as aluminum. This research examines the effect of silicate ions on the aluminum corrosion rate in a solution of sodium chloride 3.5%, tested by the potentiostat method and calculated by Tafel extrapolation. The effect of silicate ion concentration on the corrosion rate was observed in various concentrations of 0, 25, 50, 75, and 100 mM. The optimum concentration of sodium silicate in reducing the corrosion rate is 25 mM, in which the corrosion rate drops from 0.0118 to 0.0084 mm/year. Conversely, increasing the concentration of sodium silicate to 100 mM increased the corrosion rate to 0.101 mm/year.
Study of The Interaction Carbondioxide Gas with Hydroxyapatite Using Density Functional Theory Atiek Rostika Noviyanti; Azhari Yusuf; Yeni Wahyuni Hartati; Budi Adiperdana
Jurnal Keramik dan Gelas Indonesia Vol 30, No 2 (2021): Jurnal Keramik dan Gelas Indonesia
Publisher : Balai Besar Keramik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32537/jkgi.v30i2.7175

Abstract

Hydroxyapatite (HA) is a phosphate mineral with Ca10(PO4)6(OH)2 chemical formula. The hydroxyl group in HA allows ion to exchange, so this material may be applied as a CO2 gas sensor. The interaction between HA with CO2 gas is difficult to observe directly, and ab initio modeling is necessarily to investigate the interactions between HA with CO2 gas. In this study, a simulation was conducted using the density functional theory method to determine the interaction between CO2 gas on the surface of HA. The simulation results indicate that a large HA surface is required for optimal CO2 gas adsorption. Pore diameter size needs to be larger than 2.5 Å to prevent binding on two sides of the surface pores. 
A Rapid and Sensitive Diagnosis of Typhoid Fever Based On Nested PCR-Voltammetric DNA Biosensor Using Flagellin Gene Fragment Yeni Wahyuni Hartati; Santhy Wyantuti; M. Lutfi Firdaus; Nurul Auliany; Rini Surbakti; Shabarni Gaffar
Indonesian Journal of Chemistry Vol 16, No 1 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.05 KB) | DOI: 10.22146/ijc.21182

Abstract

Typhoid fever caused by Salmonella typhi is an important issue for public health in the world. Laboratory methods for rapid and sensitive diagnosis are very important for disease management. The purpose of this study was to determine the performance of nested PCR–voltammetric DNA biosensor using flagellin gene (fla) of S. typhi as a marker. The differential pulse voltammetry using pencil graphite electrode was applied to measure the guanine oxidation signal of probes vs synthetic target stDNA and probes vs fla PCR product hybridizations. The probe DNA selectivity was examined by hybridized probes vs non-complementary sequence. The result showed that the first round nested PCR product can not be visualized by agarose electrophoresis, whereas using the voltammetric biosensor methods can be detected both for the first or second round nested PCR product. The average peak current of hybridized probe vs first and second round of PCR product was 2.32 and 1.47 μA respectively, at 0.9 V. Detection of the DNA sequences of the infectious diseases from PCR amplified real sample was also carried out using this voltammetric DNA biosensor methods.
An Electrochemical Aptasensor for the Detection of HER2 as a Breast Cancer Biomarker Based on Gold Nanoparticles-Aptamer Bioconjugates Yeni Wahyuni Hartati; Sari Syahruni; Shabarni Gaffar; Santhy Wyantuti; Muhammad Yusuf; Toto Subroto
Indonesian Journal of Chemistry Vol 21, No 6 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.67124

Abstract

Inaccurate diagnoses contributes to the high mortality rate of breast cancer. Human epidermal growth factor receptor 2 (HER2) is overexpressed in breast cancer tumors at around 20–30%. This study aims to develop an electrochemical biosensor for HER2 based on a gold nanoparticle-aptamer bioconjugate (AuNP@HER2 aptamer) and investigate the interaction between DNA aptamer and HER2 using computational methods. The bioconjugate was synthesized using maleimide and polyethylene glycol as a linker. The –NH2 group of cysteamine that modified the gold electrode can form a covalent bond with the bioconjugate maleimide. The interaction of the bioconjugated aptamer with HER2 was measured electrochemically based on the [Fe(CN)6]3−/4− redox system. The limit of detection, the linear range of HER2, precision, and accuracy in this study were 1.52 ng mL–1, 0.01 to 15.0 ng mL–1, 0.1298, and 94.06%, respectively. The structure of the DNA aptamer was modeled using mFold, Assemble2, and Chimera, with the interaction between the DNA aptamer and HER2 explored by NPDock. The modeling of the aptamer with HER2 showed that electrostatic interactions dominated the attractive forces. The resulting interaction pattern can be used as a template to improve the binding energy of the aptamer, thus providing insight into the development of aptamer-based biosensors.
Co-Authors Aga Adi Masyhuri Annisa Ilma Naviardianti Annisa Ilma Naviardianti Ari Hardianto Atiek Rostika Noviyanti Azhari Yusuf Budi Adiperdana Diana Hendrati Diana Hendrati Diana Rakhmawaty Eddy Diana Rakhmawaty Eddy Diding Mandala Putra Eddy, Diana Rakhmawaty Egista Istioka Fazrin Elin Marlin Elsa Nur Utami Fadhilah, Riana Adisti Firdaus, Muhammad Lutfi Hesti Lina Wiraswati Husein Hernadi Bahti Iman Permana Maksum Irkham, Irkham Khaerani, Wulan Lia Faridah Lubis, Rubianto A. M. Lutfi Firdaus M. Lutfi Firdaus Mahendra Permadi Masyhuri, Aga Adi Muhammad Hilman Daniswara Muhammad Yusuf Nida Yumna Nurmalasari, Ratna Nurul Auliany Pratomo, Uji PUTRI, RAFIKA Rahmaniar Mulyani Ravenna Aristantia Riana Adisti Fadhilah Rini Surbakti Rizki Muharami Cedia Sari Rubianto A. Lubis Rukiah Rukiah Rustaman Rustaman Safri Ishmayana Salma Nur Zakiyyah Santhy Wyantuti Sari Syahruni Savira Ekawardhani Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar Shabarni Gaffar -, Shabarni Gaffar Shabrinna, Hanif Shauvina, Shauvina A Solihudin Solihudin Titin Sofyatin Toto Subroto Yohan Yohan Yohan Yohan Zakiyyah, Salma Nur Zalfadilah, Faizal Nur Zein, Muhammad Ihda Hamlu Liwaissunati