Article Per Year (5 Year)
p-Index From 2021 - 2026
3.128
P-Index
This Author published in this journals
All Journal Pharmaciana: Jurnal Kefarmasian Biota INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Pendidikan dan Konseling Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ners Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Integrasi Kesehatan dan Sains Jurnal Ilmiah Wahana Pendidikan Journal of Pharmaceutical and Sciences. Jurnal Abdimas Mahakam
Jekmal Malau
Program Studi Farmasi Universitas Singaperbangsa Karawang, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

Published : 19 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 19 Documents
Search

 1   2 
Comparison of Two PCR Primer Sets for In-House Validation of GHSR Gene Variation Detection Employing Artificial Recombinant Plasmid Approach Ahsanal Kasasiah; Jekmal Malau; Sekar Andjung Tresnawati; Priscinya Christiana Debora; Nur Komala Fitri; Saarah Hamidah Asmara Indratno; Asman Hitopik; Eriyanti Astika; Anisa Aula Rahma; Fikri, Al Mukhlas
Biota Vol 10 No 2 (2024): Jurnal Biota 2024
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v10i2.21166

Abstract

Stunting is a significant global public health problem caused by long-term dietary deficits that affect many children worldwide. Both environmental and genetic factors, including variants in the GHSR gene, play a crucial role in stunted growth. This study used an artificial recombinant plasmid DNA method to evaluate two primer set combinations for identifying DNA variants in the GHSR gene. Selecting suitable primer sets for identifying GHSR genetic variants linked to stunting is essential, as evidenced by PCR and sequencing techniques. The target gene, based on the GHSR reference sequence, consists of eight DNA variations (ΔQ36, G57G, P108L, L118L, R159R, C173R, D246A, and A277P). A recombinant plasmid was created by inserting a 1000 bp fragment of the GHSR gene into the pUC57 backbone. Primer sets were chosen based on their capacity to amplify these eight genetic variations and were optimized and validated using PCR methods. PCR and bi-directional sequencing verified the existence of surrounding DNA and specific single nucleotide variants (SNVs). In our study, we discovered four changes in the DNA sequence (R159R G>A, C173R T>C, D246A A>C, and A277P G>C) using the E1_F2/E1_R3 primer pair. Additionally, a new combination of primers (E1_F1/E1_R3) effectively detected seven DNA sequence mutations (ΔQ36 del CAG, G57G C>T, P108L C>T, L118L C>T, R159R G>A, C173R T>C, and D246A A>C). We have developed a new combination of forward and reverse primers to identify seven SNVs in the GHSR gene, which could serve as a diagnostic tool in clinical laboratory settings.
Effect of Mycotoxin Exposure on Stunting and Growth Impairment in Children: A Systematic Literature Review Anisa Aula Rahma; Jekmal Malau; Ahsanal Kasasiah
Jurnal Ners Vol. 9 No. 1 (2025): JANUARI 2025
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v9i1.29273

Abstract

Stunting is a critical public health concern in low- and middle-income countries (LMICs), is traditionally attributed to malnutrition and environmental factors. Emerging evidence suggests that mycotoxin exposure is a risk factor. This systematic review, conducted using PRISMA guidelines, investigates the association between mycotoxin exposure and childhood stunting/growth impairment. Twelve relevant studies (predominantly cross-sectional and prospective cohorts) are identified, focusing on children aged 0-5 years in Africa and Asia. Aflatoxins (AFB1, AFM1) and fumonisins (UFB1) are the primary mycotoxins analyzed, measured through biomarkers in biological samples (blood, urine, breast milk). Aflatoxin exposure consistently demonstrates a negative impact on child growth, as evidenced by decreasing anthropometric z-scores (LAZ, WAZ, HAZ). Additionally, it significantly increases the risk of stunting and underweight. Potential mechanisms include disruption of the IGF signaling pathway, oxidative stress, impaired protein synthesis, intestinal barrier dysfunction, and nutrient malabsorption. Fumonisins, both independently and in co-exposure with aflatoxins, are also linked to growth impairment. This review highlights the detrimental effect of mycotoxin exposure on child growth. While aflatoxin exposure appears to be a major contributor, fumonisins warrant further investigation. Elucidating the underlying molecular mechanisms and exploring interactions with other risk factors are crucial next steps. Addressing mycotoxin-related stunting necessitates a multidisciplinary approach.
Modification of black glutinous rice (Oryza Sativa var. glutinosa) starch: effect on pharmaceutical properties Devi Ratnasari; Jekmal Malau; Dhavina Maharani
Pharmaciana Vol. 16 No. 1 (2026): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v16i1.30660

Abstract

Black glutinous rice is rich in starch, specifically amylopectin by 92-98%, making it a potential pharmaceutical excipient. However, black glutinous rice starch also has disadvantages, such as poor flowability and compressibility, which necessitate modification. This study investigates the impact of various modification methods on the pharmaceutical properties of black glutinous rice starch, including its Carr’s index, flowability, loss on drying, pH, and swelling power. The starches were modified using acetylation, butanol addition, autoclave-cooling, and pregelatinization. The results showed that modified butanol and pregelatinization exhibited increases in Carr’s index, 3.4 ± 0.13% and 31.10 ± 0.18%, respectively, indicating improved compressibility. Flow rate analysis revealed that pregelatinized starch exhibited the highest flowability, followed by autoclave-cooling, butanol, and acetylated starch. The swelling power was significantly enhanced in autoclave-cooled starch (100%), likely due to structural changes induced by high-temperature treatment, increasing water absorption. The pH values of all modified starches remained within pharmaceutically acceptable ranges (4–8). Overall, starch modification, particularly autoclaving and pregelatinization, significantly enhanced the functional properties relevant to pharmaceutical applications, highlighting their potential use as excipients in solid dosage forms.
Upaya Promotif dan Preventif pada Masa Pandemi Covid-19 di Karawang Aliya Azkia Zahra; Ahsanal Kasasiah; Jekmal Malau; Indah Laily Hilmi
Jurnal Abdimas Mahakam Vol. 7 No. 02 (2023): Juli
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24903/jam.v7i02.2275

Abstract

Coronavirus Disease 2019 (Covid-19) adalah penyakit menular yang disebabkan oleh Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV- 2). Angka kasus positif yang kian meningkat, terlebih lagi di Indonesia yang sudah masuk ke dalam 30 besar negara dengan angka kasus Covid-19 yang tinggi di dunia. Pemerintah sudah berupaya untuk menanggulangi situasi pandemi ini, yaitu dengan mensosialisasikan 3M (memakai masker dengan benar, menjaga jarak dan hindari kerumunan dan mencuci tangan pakai sabun). Namun angka Covid-19 di Indonesia masih terhitung tinggi. Berdasarkan data yang dipaparkan oleh satgas Covid-19, Karawang termasuk zonasi daerah dengan risiko tinggi penyebaran Covid-19. Karawang menduduki posisi ke-6 sebagai daerah zonasi risiko tinggi. Oleh karena itu, dibutuhkan kegiatan penyuluhan kepada masyarakat tentang Covid-19 dan strategi preventif menghadapi Covid-19 yang diharapkan dapat mampu meningkatkan kesadaran masyarakat terhadap bahayanya Covid-19 serta masyarakat yang berperan penting dalam memutus rantai penyebaran Covid-19 harus selalu dihimbau untuk melaksanakan protokol kesehatan 3M dan selalu menjaga kesehatan. Dengan hasil setelah dilakukan kegiatan penyuluhan terlihat terdapat peningkatan pengetahuan masyarakat sebesar 10%.
Molecular identification and antibacterial activity of endophytic bacteria from Bambusa vulgaris leaves as antibacterial potential against phatogenic microoganism Jekmal Malau; Vriezka Mierza; Munir Alinu Mulki; Lestari Mahardika Urbaningrum; Vina Luthfiana Hasna; Priscinya Christiana Debora
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 2 (2025): From Natural Compounds to Disease Mechanisms: An Integrated Research Outlook
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i2.6538

Abstract

The increasing prevalence of antibiotic-resistant bacteria poses a critical global health concern, necessitating the exploration of novel antibacterial solutions. Endophytic bacteria, which colonize plant tissues without causing harm, have gained attention as potential sources of bioactive metabolites. This study aimed to isolate and characterize endophytic bacteria from Bambusa vulgaris leaves and evaluate their antibacterial potential against pathogenic microorganisms. Leaf samples were collected from Bekasi, West Java, and subjected to a surface sterilization process prior to bacterial isolation. A total of 12 bacterial strains were successfully obtained and screened for antibacterial activity against Bacillus subtilis, Propionibacterium acnes, Pseudomonas aeruginosa, and Staphylococcus epidermidis using the agar well diffusion assay. Three isolates exhibited notable inhibitory activity, with P8 demonstrating the strongest antibacterial effects against B. subtilis, P. acnes, and S. epidermidis. The two most potent isolates, P8 and K3, were characterized via 16S rRNA gene sequencing. Genomic DNA extraction was performed, followed by Polymerase Chain Reaction (PCR) amplification using the universal primers 27F (5′-AGAGTTTGATYMTGGCTCAG-3′) and 1492R (5′-GGTTACCTTGTTACGACTT-3′). Sequencing and The Basic Local Alignment Search Tool (BLAST) analysis confirmed that isolate P8 exhibited 100% similarity to B. subtilis strain LZH-H1, whereas isolate K3 shared 99.85% similarity with Pantoea stewartii subsp. indologenes strain SR2-12. These findings suggest that endophytic bacteria from B. vulgaris endohytic bacteria hold promise as potential sources of antibacterial compounds. Further research is necessary to purify and characterize these bioactive metabolites for potential pharmaceutical applications.
Genetic Studies On The GHSR and IGF1R and Their Relationship With Stunting: A Systematic Literature Review Saarah Hamidah Asmara Indratno; Jekmal Malau; Ahsanal Kasasiah
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i2.5731

Abstract

Stunting, also known as short stature, is a condition that occurs in children where children grow too short for their age. It can be influenced by several external and internal factors. This study explored various databases including Pubmed, Science Direct, Sage Journal, Springer Link, with keywords: "stunting OR short stature AND GHSR AND IGF1R AND genetic". There are 114 articles that match the PICOTS elements to be further reviewed using the PRISMA diagram. In this review article, 10 research articles published in 2011-2024 were used as sources of information. This review article discusses that internal factors of stunting can be caused by genetic factors influenced by growth genes, one of which is GHSR and IGF1R. Whole genome sequencing revealed potential genes involved in the growth pathway that showed an association between genetic variation in GHSR and IGF with the risk of the body's production or response to growth hormone that causes delayed child growth. The evidence highlights the possibility that these putative genetic markers could offer more effective treatment by focusing on the pathophysiology associated with development stunting. Due to lack of genetic studies on stunting genes carried out in Asian nations, genetic testing has not been used in clinical practice as a routine evaluation in the current national implementation, especially in Indonesia
Influence of LEAP2 (Liver Expressed Antimicrobial Peptide-2) and Ghrelin Binding to GHSR Gene Receptor as Factors Obesity Incidence: A Literature Review Asman Hitopik; Ahsanal Kasasiah; Jekmal Malau
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i3.5834

Abstract

Obesity is a serious issue in global health, which requires a more comprehensive understanding for the development of effective therapies. This study explores the role of Liver-Expressed Antimicrobial Peptide 2 (LEAP2) and its interaction with Ghrelin in regulating Growth Hormone Secretagogue Receptor (GHSR) as a potential obesity event. This method used in this review is a systematic literature analysis. The results obtained show that LEAP2, originally known as an antimicrobial, plays a significant role in metabolic regulation and body weight regulation. LEAP2 acts as a Ghrelin antagonist, reduces orexigenic effects and inhibits food intake, and exerts positive effects in tackling the effects of obesity such as hyperlipidemia and inflammation. The study also highlighted the potential of LEAP2 as a therapeutic target in obesity treatment, with palmitoylation modification showing increased stability and effectiveness of LEAP2. However, further understanding and clinical trial studies are needed to validate the preclinical findings and evaluate the long-term effects of LEAP2 regulation on human body weight and metabolic health, thus providing a basis for exploring potential clinical applications in future anti-obesity drug development
Gut Microbiota Types between Well-Grown and Stunted Children: A Systematic Review Eriyanti Astika; Ahsanal Kasasiah; Jekmal Malau; Al Mukhlas Fikri
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i2.5871

Abstract

Stunting, one of the malnutrition in the prenatal period up to the first two years of life and often exacerbated by recurrent gastrointestinal infections after birth. This study explores from various databases including Pubmed, Scopus, Science Direct, Sage Journal, Springer Link, with keywords: "stunting, gut microbiota, toddler". In this study, 10 open access articles published in 2016-2024 were used as sources of information using PRISMA 2020 guideline. It was found that the presence of gut microbiota plays a role in stunting. Gastrointestinal infections have specific mechanisms that can lead to impaired growth and postnatal growth. Deficiency of non-pathogenic gut microbiota is considered to be the main cause. Changes in gut bacterial composition in children under five are associated with increased gastrointestinal frequency, which in turn leads to chronic growth disorders.
Suplementasi Butirat dan Modulasi Epigenetik melalui Inhibisi Histon Deacetylase pada Diabetes Melitus Tipe 2: Suatu Tinjauan Naratif dengan Perspektif untuk Indonesia Aliza Salsabila Ainaputri; Ahsanal Kasasiah; Jekmal Malau; Cantika Aprillia; Dewi Pratiwi Purba Siboro; Endeh Apriyanti; Afif Tri Nugraha
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 1 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i1.1301

Abstract

Type 2 diabetes mellitus is a multifactorial metabolic disorder characterized by insulin resistance, chronic inflammation, and epigenetic dysregulation. Emerging evidence indicates that butirat supplementation may improve metabolic homeostasis through histon deacetylase inhibition and modulation of gene expression involved in insulin signaling and inflammatory pathways. This review aims to evaluate preclinical and clinical evidence on the role of butirat as an epigenetic modulator in type 2 diabetes mellitus and to discuss its translational relevance, including perspectives for Indonesia. A structured literature search was conducted to identify original research articles published between 2015 and 2025. The selected studies demonstrate that butirat enhances insulin sensitivity, improves glycemic control, and attenuates inflammatory responses through increased histon acetylation. However, direct clinical evidence remains limited. In Indonesia, available data are largely derived from dietary or probiotic interventions that increase endogenous butirat production. Therefore, well designed controlled clinical trials are required to evaluate the therapeutic potential of direct butirat supplementation in populations with type 2 diabetes mellitus.
Co-Authors Afif Tri Nugraha Ahsanal Kasasiah Al Mukhlas Fikri Alfina Oktavianti Aliya Azkia Zahra Aliza Salsabila Ainaputri Angel Novia Fransiska Anggi Ayu Pratama Anisa Aula Rahma Anisa Aula Rahma Anisa Fauziah Anjarwati, Sri Ari Purwanti Arline Rahmadianti Kusumawardhani Asman Hitopik Asman Hitopik Asri Yuniar Cantika Aprillia Chalisya Vanya Advaita Datu Muhammad Cordova Devi Ratnasari Dewi Pratiwi Purba Siboro Dhavina Maharani Diva Afiah Hanifa Irawan Eka Melati Elvira Julia Ariyanto Endeh Apriyanti Eriyanti Astika Eriyanti Astika Faizal Auladi Rivianto Fikri, Al Mukhlas Herdiana Verliani Indah Laily Hilmi Irene Virda Sakina Isyana S alsabila Lestari Mahardika Urbaningrum Mekar Meilisa Amalia Mochamad Galuh Ryandha Muhammad Fajri Maariz Mulidini Mulidini Munir Alinu Mulki Mutia Desvi Putri Mutiara Alfiyah Najla Yusiana Wahyudi Nissa Khalida Hanum Nofirman, Nofirman Nolla Olipia Elva Megrian Nur Komala Fitri Nurma Dwi Rahmawati Olivia Tahalele Priscinya Christiana Debora Priscinya Christiana Debora Putri Setya Tyasna Putri Wulanbirru Ramdhan Kurniawan Saarah Hamidah Asmara Indratno Saarah Hamidah Asmara Indratno Salsabila Granadha Nibullah Sekar Andjung Tresnawati Shafira Intan Anggraini Shinta Puspa Dwiyanti Siti Nafisa Siti Nuriah Siti Rohmah Sofianti Hidayat Sri Rahayu Syifa Khairunnisa Ti Aisyah Tiara Cinta Amelia Tiara Nurayuni Tintia Rafika Putri Tiwi Ambarati Vina Luthfiana Hasna Vriezka Mierza Welly Windari Weraman, Pius Yuliana Arsyad Yumareta Anggun Zuyyinna Alya Abbas