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Jekmal Malau
Program Studi Farmasi Universitas Singaperbangsa Karawang, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Upaya Promotif dan Preventif pada Masa Pandemi Covid-19 di Karawang Aliya Azkia Zahra; Ahsanal Kasasiah; Jekmal Malau; Indah Laily Hilmi
Jurnal Abdimas Mahakam Vol. 7 No. 02 (2023): Juli
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24903/jam.v7i02.2275

Abstract

Coronavirus Disease 2019 (Covid-19) adalah penyakit menular yang disebabkan oleh Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV- 2). Angka kasus positif yang kian meningkat, terlebih lagi di Indonesia yang sudah masuk ke dalam 30 besar negara dengan angka kasus Covid-19 yang tinggi di dunia. Pemerintah sudah berupaya untuk menanggulangi situasi pandemi ini, yaitu dengan mensosialisasikan 3M (memakai masker dengan benar, menjaga jarak dan hindari kerumunan dan mencuci tangan pakai sabun). Namun angka Covid-19 di Indonesia masih terhitung tinggi. Berdasarkan data yang dipaparkan oleh satgas Covid-19, Karawang termasuk zonasi daerah dengan risiko tinggi penyebaran Covid-19. Karawang menduduki posisi ke-6 sebagai daerah zonasi risiko tinggi. Oleh karena itu, dibutuhkan kegiatan penyuluhan kepada masyarakat tentang Covid-19 dan strategi preventif menghadapi Covid-19 yang diharapkan dapat mampu meningkatkan kesadaran masyarakat terhadap bahayanya Covid-19 serta masyarakat yang berperan penting dalam memutus rantai penyebaran Covid-19 harus selalu dihimbau untuk melaksanakan protokol kesehatan 3M dan selalu menjaga kesehatan. Dengan hasil setelah dilakukan kegiatan penyuluhan terlihat terdapat peningkatan pengetahuan masyarakat sebesar 10%.
Comparison of Two PCR Primer Sets for In-House Validation of GHSR Gene Variation Detection Employing Artificial Recombinant Plasmid Approach Ahsanal Kasasiah; Jekmal Malau; Sekar Andjung Tresnawati; Priscinya Christiana Debora; Nur Komala Fitri; Saarah Hamidah Asmara Indratno; Asman Hitopik; Eriyanti Astika; Anisa Aula Rahma; Fikri, Al Mukhlas
Biota Vol 10 No 2 (2024): Jurnal Biota 2024
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v10i2.21166

Abstract

Stunting is a significant global public health problem caused by long-term dietary deficits that affect many children worldwide. Both environmental and genetic factors, including variants in the GHSR gene, play a crucial role in stunted growth. This study used an artificial recombinant plasmid DNA method to evaluate two primer set combinations for identifying DNA variants in the GHSR gene. Selecting suitable primer sets for identifying GHSR genetic variants linked to stunting is essential, as evidenced by PCR and sequencing techniques. The target gene, based on the GHSR reference sequence, consists of eight DNA variations (ΔQ36, G57G, P108L, L118L, R159R, C173R, D246A, and A277P). A recombinant plasmid was created by inserting a 1000 bp fragment of the GHSR gene into the pUC57 backbone. Primer sets were chosen based on their capacity to amplify these eight genetic variations and were optimized and validated using PCR methods. PCR and bi-directional sequencing verified the existence of surrounding DNA and specific single nucleotide variants (SNVs). In our study, we discovered four changes in the DNA sequence (R159R G>A, C173R T>C, D246A A>C, and A277P G>C) using the E1_F2/E1_R3 primer pair. Additionally, a new combination of primers (E1_F1/E1_R3) effectively detected seven DNA sequence mutations (ΔQ36 del CAG, G57G C>T, P108L C>T, L118L C>T, R159R G>A, C173R T>C, and D246A A>C). We have developed a new combination of forward and reverse primers to identify seven SNVs in the GHSR gene, which could serve as a diagnostic tool in clinical laboratory settings.
Co-Authors Ahsanal Kasasiah Alfina Oktavianti Aliya Azkia Zahra Angel Novia Fransiska Anggi Ayu Pratama Anisa Aula Rahma Anisa Fauziah Anjarwati, Sri Ari Purwanti Arline Rahmadianti Kusumawardhani Asman Hitopik Asri Yuniar Chalisya Vanya Advaita Datu Muhammad Cordova Diva Afiah Hanifa Irawan Eka Melati Elvira Julia Ariyanto Eriyanti Astika Faizal Auladi Rivianto Fikri, Al Mukhlas Herdiana Verliani Indah Laily Hilmi Irene Virda Sakina Isyana S alsabila Mekar Meilisa Amalia Mochamad Galuh Ryandha Muhammad Fajri Maariz Mulidini Mulidini Munir Alinu Mulki Mutia Desvi Putri Mutiara Alfiyah Najla Yusiana Wahyudi Nissa Khalida Hanum Nofirman, Nofirman Nolla Olipia Elva Megrian Nur Komala Fitri Nurma Dwi Rahmawati Olivia Tahalele Priscinya Christiana Debora Putri Setya Tyasna Putri Wulanbirru Ramdhan Kurniawan Saarah Hamidah Asmara Indratno Salsabila Granadha Nibullah Sekar Andjung Tresnawati Shafira Intan Anggraini Shinta Puspa Dwiyanti Siti Nafisa Siti Nuriah Siti Rohmah Sofianti Hidayat Sri Rahayu Syifa Khairunnisa Ti Aisyah Tiara Cinta Amelia Tiara Nurayuni Tintia Rafika Putri Tiwi Ambarati Welly Windari Weraman, Pius Yuliana Arsyad Yumareta Anggun Zuyyinna Alya Abbas